1.Progress of lentiviral vector on neoplasms gene therapy research
Journal of International Oncology 2014;41(6):422-424
Lentiviral vectors (LV),a special member of retroviral vectors,has become hot in gene therapy research.Studies have shown that LV can make exogenous gene expressed stably in tumor cells.As a tool for transportation,LV plays a significant role in the gene therapy of digestive system,hematologic system and gynecological tumor,which provides a promising new way for neoplasms gene therapy.
2.Peroxisome proliferator-activated receptor-gamma coactivator 1 and skeletal muscle adaptation
Lei DENG ; Song WANG ; Jie NIU
Chinese Journal of Tissue Engineering Research 2010;14(11):2022-2025
BACKGROUND:An increase in activity of muscle contraction can induce transcription of a variety of signaling molecules to activate a large number of gene expression within the nucleus through proprietary signaling pathway OBJECTIVE:To review studies related the peroxisome proliferator-activated receptor-gamma coactivator 1(PGC-1)and exercise induced skeletal muscle adaptations.METHODS:A computer-based online search of Pubmed was performed for relevant English articles published from January 1995 to January 2009 with the keywords of"PGC1,skeletal muscle,exercise,adaptations"The relevant articles about PGC-1 and exercise induced skeletaI muscle adaptations were included.and repetitive contents were excluded.PGC1 and mitochondrial oxidative metabolism,and exercise-induced skeletal muscle adaptation and PGC1s changed served as the evaluation indicators.RESULTS AND CONCLUSION:Of 57 collected articles,34 were classified and sorted according to the criteria.Endurance training results in profound adaptations for skeletal muscle,including mitochondrial biogenesis,capillary density,and fiber composition.Transcription factors are highly dependent on coactivator molecules to regulate training physiologic adaptation processes.The majority of transcription factors target genes are involved in mitochondrial biogenesis and metabolism.These transcriptional patterns may provide a basic framework for understanding the integration of mitochondrial biogenesis and function with signaling events that exercise induced energetic properties Post-transcription of PGC-1 alpha protein,there are a variety of protein modifications,with a variety of biological processes closely related to the possible adaptive mechanism of exercise-induced skeletal muscle.
3.Treatment of posterior dislocation and acetabular posterior column (wall) fracture with greater trochanteric os-teotomy via Kocher-Langenbeck approach
Huibin NIU ; Zhongguo LIU ; Gangxi DENG
Journal of Regional Anatomy and Operative Surgery 2014;(1):51-52,55
Objective To introduce the operation method and curative effect of posterior dislocation and acetabular posterior column ( wall) fracture with greater trochanteric osteotomy via Kocher-Langenbeck approach. Methods 14 cases of posterior dislocation and ace-tabular posterior column( wall) fracture who were treated by greater trochanteric osteotomy via Kocher-Langenbeck approach were collected, and the surgical methods, intraoperative situation and postoperative recovery were analyzed. Results The mean operating time is 105 min and the mean bleeding volum is 600 mL. After operation, there were 5 cases of anatomical reduction;8 cases of good reduction;1 cases of unsatisfactory reduction. Among the patients, 7 cases were of excellent clinical effect;4 cases were of good clinical effect, 3 cases were of medium clinical effect. Heterotopic ossification occurred in 3 cases and traumatic arthritis occurred in 4 case. Both acetabular posterior col-umn ( wall) and trochanter osteotomy ends were healed, and there was no iatrogenic sciatic nerve and femoral head necrosis injury. Conclu-sion In treatment of posterior dislocation and acetabular posterior column ( wall) fracture, greater trochanteric osteotomy via Kocher-Lange-nbeck approach can provide excellent exposure, and it is conducive to the reduction and fixation of fracture and dislocation.
4.Effect of salidroside on mitochondrial membrane potential during injury induced by hypoxia/hypoglycemia in cultured SH-SY5Y cells
Wensheng ZHANG ; Lingqun ZHU ; Ruichun DENG ; Fuling NIU ; Rong TIAN
Chinese Journal of Pathophysiology 2000;0(07):-
AIM: To investigate the effects of salidroside on intracellular free calcium concentration (([Ca~(2+)]i)), apoptosis, mitochondrial membrane potential (MMP) and activity during injury induced by hypoxia/hypoglycemia in cultured SH-SY5Y cells. METHODS: Mitochondrial activity was measured by methylthiazolyl tetrazolium test. MMP, [Ca~(2+)]i and apoptosis were measured by flow cytometry. RESULTS: SH-SY5Y cells were cultured in a hypoxia/hypoglycemia condition for 2, 4, 6 and 12 h, [Ca~(2+)]i and apoptosis rate significantly increased compared with control group (P
5.Neuropathulogical study on epilepsy in organotypic slice model
Jinbo DENG ; Shanting ZHAO ; Guimin WANG ; Yanli NIU
Chinese Journal of Neurology 2008;41(8):547-551
Objective In order to make a epileptic model in organotypic slice culture and to further investigate the epileptic pathology. Methods Immunohistochemistry, Fluoro-Jade B staining and BrdU labeling were carried out in the hippocampal slices with domoic acid treatment after 7 days of culture.Results Domoic acid could induce a series of pathological changes, such as, dispersion of granule cells(0.105±0.063) mm vs (0.057±0.012) mm, t = 4.8, P<0.01), neurogenesis in granular layer and cell loss of pyramidal cell and Mossy cell as well. Conclusions In hippocampal organotypic slices, domoic acid induced such pathological changes as human temporal lobe epilepsy and epileptic animal model. It is an ideal epileptic model for pathological, pharmacological and electronic physiological studies with great applicable prospect.
6.EFFECT OF COLD-PRESSED CAMELINA SATIVA OIL ON HYPERLIPEMIA OF RATS INDUCED BY HIGH FAT DIET
Qianchun DENG ; Fenghong HUANG ; Qingde HUANG ; Changsheng LIU ; Yanxing NIU
Acta Nutrimenta Sinica 1956;0(04):-
Objective To investigate the effect of cold pressed Camelina Sativa oil (CPCO) on growth performance and lipid metabolism of hyperlipemia rats. Method The rats were divided into control group (Cont),high fat diet group (HFD),fish oil group (HFD+FO),low dose CPCO group (HFD+LCPCO) and high dose CPCO group (HFD+HCPCO). The latter 4 groups were fed high fat feed. Fo and CPCO were given by gavage. Total cholesterol (TC),triglyceride (TG),high density lipoprotein cholesterol (HDL-C) and HDL-C subtype levels in serum were determined at the end of 6w. The distribution of HDL-C particles was studied by agarose gel electrophoresis. Results HFD resulted typical hyperlipemia. Body weight,TC and TG level were significantly decreased,and HDL-C particles tended to be miniaturized and the HDL3 level increased by feeding Camelina Sativa oil as compared with HFD group. TC content in HFD+FO and HFD+HCPCO group was decreased 46.06% and 30.60% respectively. TG content in these two groups was decreased 25.81% and 22.72% respectively as compared with HFD group. Conclusion Chinese Camelina Sativa oil which contained high level of ?-linolenic acid(ALA)could prevent and cure hyperlipidemia by regulating blood lipid metabolism and protecting hepatic function.
7.Values of MRE in diagnosis of stages of hepaticfibrosis:A Meta-analysis
Meng NIU ; Dayong DENG ; Yunpengfei LI ; Shuo LIU ; Jun DING
Journal of Jilin University(Medicine Edition) 2017;43(4):787-793
Objective:To investigate the efficacy and the clinical value of magnetic resonance elastography(MRE) in diagnosis of hepatic fibrosis with Meta-analysis, and to provide basis for clinical treatment of hepatic fibrosis.Methods:The studies published before February 2, 2017 about MRE and staging of hepatic fibrosis in Chinese or English were retrived in the databases including PubMed, EMBase, Web of Science, Cochrane Library,CNKI, CBMDisc,VIP, Wanfang data, and supplemented by manual retrieval for relevant literatures.The inclusion and exclusion criterions were used to select and extract the literatures.The literatures qualitie were valuated based on QUADAS-2 tool.The sensitivity(SEN), specificity (SPE), diagnostic odds ratio (DOR), positive likelihood ratio (+LR), negative likelihood ratio (-LR) on the groups of F0 vs F1-F4,F0-F1 vs F2-F4,F0-F2 vs F3-F4, F0-F3 vs F4 and heterogeneity were combined and tested with Stata software respectively.HSROC and AUROC were also implemented.Results:A total of 1 332 studies were searched, and 22 were included.21 of them were in English and 1 in Chinese.The results of Meta analysis showed that the SENp, SPEp, +LRp,-LRp, DOR and AUROC in F0 vs F1-F4 group were 88.8%(85.0-91.7),95.9%(91.5-98.0),21.435(10.215-44.979),0.117(0.086-0.159),183.187(72.533-462.650) and 0.96(0.94-0.98) ,respectively;the SENp, SPEp, +LRp,-LRp, DOR and AUROC in F0-F1 vs F2-F4 group were 93.3%(89.2%-35.9%), 94.1%(90.2%-96.5%),15.839(9.344-26.848),0.072(0.044-0.117),221.224(100.980-484.648) and 0.98(0.96-0.99),respectively;the SENp, SPEp, +LRp, -LRp, DOR and AUROC in F0-F2 vs F3-F4 group were 92.9%(88.9%-95.5%),94.6%(91.2%-96.8%),17.348(10.496-28.671),0.075(0.048-0.119),230.434(111.482-476.317)0.98(0.96-0.99), respectively;the SENp, SPEp, +LRp,-LRp, DOR and AUROC in F0-F3 vs F4 group were 97.7%(93.0%-99.3%),93.2%(90.3%-95.2%),14.337(9.910-20.742),0.025(0.008-0.075),580.405(144.871-2325.307) and 0.98(0.96-0.99),respectively.Conclusion:MRE,as a new and noninvasive imaging method, has high diagnostic value in all stages of hepatic fibrosis, which can provide a reliable reference for clinical precise treatment of hepatic fibrosis.
8.Verification of the clinical applicability of the published standard reference interval:a retrospective study based on health examination results of Han and Uygur nationality
Huimin LIU ; Xue SONG ; Zhaohui DENG ; Shumin NIU ; Xueli LIANG ; Tiesi BA ; Yila RE ; Xin ZHANG
Chongqing Medicine 2016;(1):8-10
Objective To verify the clinical applicability of the published standard intervals (WS/T402-2012) for WBC , RBC ,PLT and Hb based on the health examination results of Han and Uygur populations in Urumqi .Methods This was a retro-spective study .The results of WBC ,RBC ,PLT and Hb from health examination individuals of Han and Uygur populations from August 2013 to January 2015 were collected ,9 307 health subjects age range from 20 to 79 years of the two nationalities were cho-sen using health examination information system .The percents of health subjects failed falling in the published standard interval were calculated to verify the judgment criterion ,fail falling rate < 10 .00% was regard as qualified .Results The test of normality revealed that the Han and Uygur′s results of all verified items were skewed distributions .The 2 .5% and 97 .5% percentiles of the results of two nationalities :WBC Han(3 .6 ~ 9 .6) × 109 /L ,Uygur(3 .8 ~ 9 .9) × 109 /L ;RBC Han Male (4 .3 ~ 5 .9) × 1012 /L and Female (3 .9 ~ 5 .2) × 1012 /L ,Uygur Male (4 .4 ~ 5 .8) × 1012 /L and Female (3 .9 ~ 5 .2) × 1012 /L ;PLT Han(130 ~ 351) × 109 /L , Uygur(145 ~ 370) × 109 /L) ,and Hb Han :Male (133 ~ 178)g/L and Female (111 ~ 153)g/L ,Uygur :Male (133 ~ 174) g/L and Fe-male (110 ~ 152)g/L .The significant differences of each testing item were found in the different sex from the same nationality(P<0 .01) .The percents of health subjects failed falling in the published standard interval were < 10 .00% in both Han and Uygur na-tionality .Conclusion The published standard reference intervals for WBC ,RBC ,PLT and Hb are applicable to our laboratory for the detection of Han and Uygur populations .
9.Meta analysis of Orem self-care mode on well-being among patients with schizophrenia
Zijun LIAO ; Tengfei NIU ; Fan ZHANG ; Peng DENG ; Xiaojun TANG ; Yong ZHAO ; Yang WANG
Chongqing Medicine 2014;(31):4200-4202
Objective To evaluate the effect of Orem self‐care mode on sense of well‐being in patients with schizophrenia by Me‐ta analysis .Methods The databases of CBM ,VIP ,CNKI and Wan Fang Data were searched .All randomized controlled trials(RCT ) about Orem self‐care mode for patients about sense of well‐being were included .Data collection and literature review were per‐formed by two reviewers independently .The RevMan 5 .2 software was taken for analysis .Results Three literatures were includ‐ed .All these articles were regarded as low quality ,and only one article′s score of methodological quality are more than three .The Meta analysis showed there were significant differences between the Orem self‐care mode group and the normal nursing mode group in sense of well‐being .Besides the scores of the Orem self‐care mode group are higher(MD = 16 .29 ,95% CI(14 .54 - 18 .05) ,P<0 .05) .Conclusion The Orem self‐care mode is better than normal nursing for sense of well‐being in patients .However ,trials are only three ,and most trials included in the review are of low quality .So large scale randomized controlled trials of higher quality are needed to confirm the result .
10.A Clinical Study on Imbalance of Th17/Treg in Patients with Ovarian Endometriosis
Juan YANG ; Chenghou WEI ; Caixia ZHU ; Huizhen GENG ; Songqing DENG ; Gang NIU
Journal of Sun Yat-sen University(Medical Sciences) 2017;38(1):95-100
[Objective]To investigate the disturbance between Th17 and Treg cell balance in ovarian endometriosis patients.[Methods]Case-control study comparing 40 women with histo-pathologically confirmed ovarian endometriosis and with 40 control infertility women without visible endometriosis foci ,pelvic inflammations who were subjected to laparoscopic surgery during the same period. Peripheral blood,peritoneal fluid,ovarian ectopic endometrial tissue and eutopic endometrial tissue of ovarian endometriosis patients and controls were collected during surgery. T lymphocytes subpopulations in peripheral blood were analyzed by flow cytometry using specific monoclonal antibodies recognizing CD4+,CD25+and CD127-markers and CD3+,CD8-and IL-17A+markers. Then, IL-17,IL-22,IL-10and TGF-βconcentration in the serum and peritoneal fluid was determined using enzyme linked immunosorbent assay(ELISA). Also,Q-PCR was performed to verify Foxp3 mRNA and ROR-γt mRNA expression differences in eutopic and ectopic endometrial tissue.[Results]1.The percentage of CD4+CD25+CD127-Treg cells was significantly decreased in the peripheral blood ofwomen with ovarian endometriosis compared with control women. On the other hand ,the proportion of CD3+CD8-IL-17A+Th17 cells was significantly increased in the peripheral blood of women with endometriosis compared with control wom en. 2. Comparing with the controls ,the concentration of IL-17 and IL-22 was significantly higher in the serum of women with ovarian endometriosis ,and the levels of IL-10 and TGF-β were significantly lower in the serum of women with endometriosis. On the contrast ,in the peritoneal fluid of women with ovarian endometriosis ,the concentration of IL-17 and IL-22 were lower ,and the concentration of IL-10 and TGF-β were significantly higher than the controls. 3.Foxp3 mRNA expression level was significantly elevated in ectopic endometrial tissue of patients with ovarian endometriosis compared with eutopic endometrial tissue ,while the ROR-γt mRNA expression level of ectopic endometrial tissue was significantly decreased than eutopic endometrial tissue.[Conclusion]The present study verifies the imbalance of Th17/Treg in peripheral blood ,peritoneal fluid and endometrial tissue in ovarian endometriosis patients ,which implies the immune dysregulation and the disturbance of immunity homeostasis in the establishment and progression of endometriosis.