Objective To study the sleep quality of the parents whose children with cerebral palsy (CP).Methods The Pittsburgh sleep quality index (PSQI) as an investigative tool was used to investigate 36 cases of parents with CP children and 36 cases of parents with normal children.Results The sleep problem report rate was 34.72％ in parents with CP children,and 19.44％ in parents with normal children,their difference was statistically significant (x2 =4.255,P ＜0.05).Score of PSQI(5.72 ±3.54 vs 3.19±2.76,t =3.380,P ＜0.01),sleep quality(1.33 ±0.83 vs 0.78 ±0.34,t =3.371,P ＜0.01),fall asleep time (1.28 ± 0.88 vs 0.72 ± 0.36,t =3.027,P ＜ 0.01),hours of sleep (1.16 ± 0.72 vs 1.01 ±0.62,t =2.278,P ＜ 0.05),and insomnia(1.23 ± 0.56 vs 0.75 ± 0.28,t =2.949,P ＜ 0.01) of parents with CP children were significantly different from the parents with normal children.Score of PSQI (6.21±0.85 vs4.32 ±0.73,t =3.380,P ＜0.01),sleep quality(1.14 ±0.73 vs 0.89 ±0.66,t =2.986,P＜ 0.01),fall asleep time (1.22 ± 0.81 vs 0.96 ± 0.83,t =2.853,P ＜ 0.01),hours of sleep (1.09 ± 0.66vs 0.85 ± 0.71,t =2.136,P ＜ 0.05),insomnia (1.15 ± 0.63 vs 0.83 ± 0.62,t =2.513,P ＜ 0.01)and daytime function(1.19 ± 0.43 vs 0.88 ± 0.62,t =2.586,P ＜ 0.01) of mothers with CP children were significantly different from fathers with CP children.Conclusions The sleep quality of parents of CP children are worthy of attention.

Objective To explore the treatment of patients with severe traumatic shock first-aid and the preventive measure of the complications caused by a large number of fast blood transfusion during surgery.Methods Retrospective analysis of 87 patients with severe traumatic shock and rapid blood transfusion given to a large number of patients for emergency medical treatment, while the load cycle, bleeding, body temperature, serum potassium, and other indicators were observed, effects and complieations of treatment and effective prevention measures were summarized.Results 87 patients after aggressive surgical treatment,the success rate was 81.61% (71/87) ;surgery circulatory overload caused massive blood transfusion, bleeding tendency,low body temperature,low blood potassium disorders such specific complications.Conclusion Patients with severe traumatic shock should be taken promptly and effective first aid treatment and.the complication caused by massive blood transfusion during surgery,and it could reduce mortality and complications.

AIM: To set up identification of Flos Lonicerae and determination of chlorogenic acid in Yingqiao jiedu Pills (Flos Lonicerae, Fructus Forsythiae, Radix Glycyrrhizae, Herba Menthae, etc.) by HPLC. METHODS: A YWG C 18 column was used with the mobile phase consisted of CH 3OH 10%Na 2HPO 4 (adjusting pH to 2.7 with HAc) as gradient elution. The flow rate was 1.0mL?min -1 , and the detection wavelength was at 326nm. RESULTS: Flos Lonicerae in Yinqiaojiedu Pills was identified by HPLC UV. The linear range of chlorogenic acid was 5.0～60?g?mL -1 ( r =0.9999). The average recovery was 98.71% with RSD of 1.55% ( n =5). CONCLUSION: The method is simple, accurate and rapid with good reproducibility. It can be used for the quality control of Flos Lonicerae in Yinqiaojiedu Pills.

Teaching is very important for anesthesiology developmemt,so residents should be cultivated the teaching capacity as soon as possible.This article discussed how to cultivate the teaching capacity of residents in anesthesiology department.

AIM: To extract Zanthoxylum oil from the seeds of Zanthoxylum bungeanum Maxin, and find out a better extraction method and its optimized operating conditions. METHODS: Zanthoxylum oil was extracted by distillation method, solvent extraction and supercritical fluid extraction, respectively. The products gained in each experiment was analyzed by GC after it had been methyl esterified. RESULTS: The yield of Zanthoxylum oil extracted by distillation, solvent extraction and supercritical fluid extraction was 0.88 %, 13.73 % and 13.52 %, respectively, and its content of unsaturated fatty acid 4.50 %, 65.97 %, 74.97 %, respectively. CONCLUSION: Supercritical fluid extraction was the better of the three mehtods, whose optimized operating conditions consisted of 30 MPa pressure, 50 ?C operating temperature and 5 h extraction time.

OBJECTIVE To analyze the risk factors and antimicrobial resistance pattern of Stenotrophomonas maltophilia isolated from 6 hospitals in Beijing,and study the difference in resistance rate of isolates from different hospitals. METHODS In this study,145 cases with S.maltophilia infection were analyzed and their susceptibility was tested.The synergetic screening test was applied to detect metallo-?-lactamases. RESULTS Most of 145 strains were isolated from sputum(86.2%),mainly from ICU(48.3%),and department of respiratory medicine(22.1%).The drug sensitivity tests in vitro showed these strains were resistant to commonly used antibiotics,and drugs whose sensitive rate was over 50% included doxycycline,gatifloxacin,cefoperazone-sulbactam,levofloxacin,compound sulfamethoxazole,ceftazidime-clavulanate and ticarcillin-clavulanate.The antimicrobial resistance of strains isolated from different wards showed some difference.Of all the infected patients,56.7% had underlying diseases;92.2%were treated previously with broad-spectum antibiotics;76.8% underwent invasive examination or treatment. CONCLUSIONS S.maltophilia is resistant to many kinds of antibiotics.The infection caused by S.maltophilia often occurs in patients with severe underlying disease and low immunity.Antibiotics should by chosen by drug sensitivity tests.

OBJECTIVE To investigate probationer nurses' knowledge about nosocomial infection (NI) and clean operating-room. METHODS Survey among probationer nurses was performed by self-designed questionnaires. RESULTS 47% probationer nurses thought it very necessary to strength knowledge about NI before probation. The scores of clean operating-room knowledge was lower than that nosocomial infection (NI); The cognition rate among high educated probationer nurses were higher than low educated,P

Telomerase, as a factor of tumour's happening and development, is a kind of cell element not existing or with low activity in normal cells. Moreover, the activity of telomerase is a common channel for cell's immortalization and deterioration, and is also the main factor in cloning tumour and developing malignant turnour. Therefore, the tumour gene therapy focusing on telomerase has become the new target of present study of tumor.This therapy has greater prospects on clinical application than the past tumour gene therapy simply directing at one cancer gene.

BACKGROUND: Spine has a variety of morphological structures and complicated adjacent location, so the surgery to treat spinal abnormality is a difficult. Three-dimensional (3D) printing technology can transform 3D digital model into physical model, which has been widely used in the field of medicine, even popularized gradually in spine surgery. OBJECTIVE: To summarize the application and status of 3D printing technology in spine surgery, and to make aprospect. METHODS: The first author retrieved CNKI, Baidu Xueshu, Google Scholar, WanFang and PubMed databases for the articles published from 2010 to 2016 using the keywords of 3D printing technology, spine surgery, navigation template,spine physical model, pedicle screw, individualized implants, clinical teaching in Chinese and English, respectively.Finally 29 eligible literatures were enrolled for further analysis in accordance with the inclusion and exclusion criteria.RESULTS AND CONCLUSION: 3D printing technology has been extensively applied in spine surgery, and shows rapid development in preoperative diagnosis, intraoperative navigation, communication, teaching, graft production, implant customization, bone tissue engineering and other aspects. With the development of society, and medical imaging, 3D printing technology will be more promising in the field of medicine, especially in spine surgery

Objective To summarize the data of newborn screening in Yongchuan district of Chongqing to promote its quali‐ty ,and to provide some measurements to enhance the management of newborn screening in this area .Methods According to the management system and technical standards of newborn screening (2010 Edition) ,newborn screening in Yongchuan district was im‐plemented .Screening rate ,coverage rate of newborn screening in maternity hospital ,recall rate of suspiciously affected newborns and rate of treatment of patients were analyzed from 2013-2014 .Results The screening rate ,coverage rate of newborn screening in maternity hospital ,recall rate of suspiciously affected newborns were increased by 77 .32% ,88 .57% and 17 .44% ,respectively , during these 10 years .All patients were treated appropriately (100% ) .Congenital hypothyroidism and phenylketonuria were screened .Since 2010 ,glucose‐6‐phosphate dehydrogenase deficiency and congenital adrenal hyperplasia were added to the newborn screening test panel .Conclusion Strengthen the management ,establish administrative department ,faculty training ,health education and government financial supports are contributed to improving the quality of newborn screening .