Objective　To explore the effect of the psychological intervention on the treatment of dental anxiety (DA).Methods Measured with Corah's dental anxiety scale , 316DA patients were selected from department of dentistry and were divided into two groups: the intervention group and the control group . The psychological intervention as well as regular dental therapeutics were applied to the patients in the intervention group , whereas only regular dental therapeutics were used to those in the control group.Results　The patients had a lower clinical dental anxiety in the intervention group , significantly different from that of the control group ( P＜ 0.01). After the treatment , more patients in the intervention group had a slower pulse than those in the control group and fewer had a faster pulse .The results also showed that the psychological intervention had a better curative effect on male patients than female ( P＜0.05). Conclusion　The psychological intervention was efficient to patients with dental anxiety.

AlM: To investigate the risk factors of diabetic retinopathy ( DR) in type 2 diabetes patients.METHODS:A total of 380 cases of type 2 diabetes were selected from January 2014 to June 2014, divided into DR group (126 cases) and non-diabetic retinopathy ( NDR) as control group (254 cases), all patients were underwent medical history, physical examinations, laboratory and assistance examinations. The prevalence and single factor and multiple factors of DR were analysed by the logistic regression model.RESULTS: The univariate logistic regression analysis showed that DR was associated with diabetes duration, systolic blood pressure, triglyceride, total cholesterol, low - density lipoprotein, urine protein, intraocular pressure, carotid intima - media thickness, peripheral neuropathy disease was the related risk factors for DR. ln multiple logistic regression analysis, only duration was the related risk factors for DR.CONCLUSlON:The occurrence of DR is the outcome of combined action of multiple factors, duration is an independent risk factor for occurrence of DR.

Objective:To survey the prevalence of internet addiction disorder(IAD)of vocational college freshmen and analyze its relationship to their childhood abuse and neglect.Methods:IAT,SCL-90 and CPANS were used to investigate 740 freshmen of Yongzhou Technical Vocational College.Results:The rate of IAD among 740 freshmen is 3.2%.The factor scores of IAD students were significantly higher than those of normal students(P

Promoting bilingual teaching is an important part of reforms in higher education and also a need for training high-quality medical talents.This article discusses practice of bilingual teaching of human parasitology and analyzes the problems in teaching.

Objective To investigate the relationship between the serum level of AFP ,types of DNA ploidy and 5 year survival rate ,survival time in patients with primary hepatic carcinoma (PHC) in Boluo area in Huizhou City ,and evaluate the prognosis val‐ue of these indexes in PHC .Methods 80 cases with PHC were enrolled in observation group and divided into grade I ,II ,III and IV according to pathological grading .Other 80 cases of healthy individuals ,conducted physical examination ,were enrolled in control group .Peripheral venous blood samples (2 mL) were collected from all subjects .The serum level of AFP and types of DNA ploidy were measured by electrochemiluminescence immunoassay (ECLIA) and flow cytometry ,respectively .Results The serum levels of AFP were significantly higher in patients in different pathological grade groups compared to that in the control group (P<0 .05) .In addition ,accompany increase of pathological grade ,the levels of AFP in patients with PHC were increased .The increased level of AFP ,difference of DNA ploid types and pathological grade of PHC contributed great influences on the 5 year survival rate and sur‐vival time of patients .Conclusion The serum level of AFP and the DNA heteroploid rate may play important roles in the early di‐agnosis and assessing prognosis of patients with PHC in Boluo area in Huizhou City .

Objective:To study the preventive and therapeutic effects of Acalypha decoction on 2,4,6-trinitrobenzene sulfonic acid induced ulcerative colitis(UC) in rats.Methods: Totally 70 male SD rats were equally randomized into 7 groups, and UC was induced by intrarectal administration of TNBS (125 mg/kg in 50% ethanol). Six hours later the rats were given 3 different doses of Acapha decoction (2.5, 6.5, 9.5 g crude drug/kg body weight) daily for 10 d.Some rats were pretreated with Acalypha decoction (6.5 g crude drug/kg body weight) 3 d before the colitis was induced. Sulfasalazine was used as the standard drug for comparison. The efficacy of Acalypha decoction was assessed through the following parameters: bodyweight loss, diarrhea and bloody stool, macroscopic score,histological changes,and MPO activity in colon tissue. Results: Acalypha decoction treatment(9.5 g/kg, 6.5 g/kg) and prevention administration (6.5 g/kg) significantly inhibited the bodyweight loss and occurrence of diarrhea and rectal bleeding in the rats(P

In inflammatory bowel disease, the experimental model s have been proven to be important tools to investigate the potential therapeuti c agent and to study the mechanism of pathogenesis. Literatures available were r eviewed on the methods of induction, characteristic and availability of all kind s of chemically induced IBD animal models. It would provide some information for selection of the experimental model in pharmacology and pathogenesis study.

Objective To observe the effect of apatinib on the invasion ability and epithelial-mesenchymal transition (EMT) of non-small cell lung cancer (NSCLC) cell line A549 and H460.Methods Different doses of apatinib were incubated with the A549 and H460 cells,then the effect of apatinib on the invasion of A549 cells was detected by Transwell assay,and the change of cell morphology was observed by optical microscope,and the expression of EMT related proteins were detected by Western blotting.Results The cell viability of A549 and H460 have no change when the concentration of apatinib was under 120 nmol·L-1.Therefore,60 nmol·L-1 and 120 nmol·L-1 apatinib were used in the further experiments.The invasion of A549 cells was reduced after apatinib incubation for 48 h,with cell morphology changes,and the expression of E-cadherin was enhanced,with the depresion of N-cadherin,Vimentin and Snail,which were related to the progress of EMT.Conclusion Apatinib could induce the MET,and reduce the invasion ability of A549 and H460 cells.

Objective:Through the analysis of the basic situation of the medical service of the psychiatric inpatients in Beijing from 2003 to 2015,it provided the basis for rational allocation of mental health resources and reasonable utilization of medical expenses.Methods:Descriptive statistical method was used to analyze the annual discharged patients,average hospitalization days and hospitalization expenses of inpatients with mental illness.Results:The annual discharged patients were increasing gradually.The number of discharged patients increased from 7861 to 24898 during 2003-2015.The annual average growth rate was 10.08％.The average length of stay in hospital since 20ll was lower than before,which were about 40 days in recent years.The average hospitalization expenses per day were also increasing.It increased from 131.04 yuan to 461.02 yuan during 2003-2015.The annual average growth rate was 11.05％.Conclusion:The demand of mental illness patients for hospitalization was gradually increasing.The hospitalization demand of patients with mental illness was increasing.The medical expenses quickly increased.It needed to adjust the payment policy to promote the rational and effective utilization of medical expenses.

Objective To investigate the effect of geldanamycin(GA) on the expression of HSP(HSP90,HSP70,HSP27) and oncoprotein(mutant p53,CDK4) in human breast cancer cell line MDA-MB-435s.Methods Proliferation of MDA-MB-435s cells was measured with MTT assay.The mRNA expression levels of HSP90,HSP70 and HSP27 were detected by RT-PCR.The protein expression levels of HSP90,HSP70,HSP27,mutant p53 and CDK4 were detected by Western blot.Results GA inhibited the proliferation of MDA-MB-435s cells in a time-dose dependent manner.The expression level of mRNA and protein of HSP90,HSP70 and HSP27 were increased,and the increasing of HSP70 was the most significant,while the protein expression level of mutant p53 and CDK4 in MDA-MB-435s cells were reduced obviously after 48-hour treatment with 400 nmol/L GA as compared with that of control cells.Conclusion GA inhibits the proliferation of MDA-MB-435s cells by down-regulating the level of mutant p53 and CDK4 protein,and GA involves in the protection of cell stress through increasing the expression of HSP90,HSP70 and HSP27.