Objective To compare the efficacy of postoperative chemotherapy in combination with radiotherapy and simple radiotherapy in malignant glioma.Methods 82 patients with malignant glioma resection patients were randomly divided into observation group and control group,there were 41 patients in each group.The control were treated with simple three-dimensional conformal radiotherapy.The observation group were given temozolomide chemotherapy on the basis of three-dimensional conformal radiotherapy.The effects and adverse reactions were observed.Results Total effective rate of observation group was 63.4％ (26/41),significantly higher than that in the control group 34.1％ (14/41) (x2 =8.2439,P ＜ 0.01);gastrointestinal adverse reaction rates of the observation group was 100.0％,significantly higher than 58.5％ of the control (P ＜ 0.01),the difference of bone marrow suppression in two groups had no statistically significant (P ＞ 0.05).Conclusion Although with a higher incidence of gastrointestinal reactions,postoperative chemotherapy with radiotherapy have reliable effect,it is worth using in the condition of appropriate and timely symptomatic and supportive treatment.

Objective:To observe therapeutic effect of maleic acid levamlodipine (amlodipine) on inflammatory fac‐tor levels in patients with essential hypertension (EH) .Methods :According to random number table method ,a to‐tal of 84 EH patients were randomly and equally divided into nifedipine group (received nifedipine treatment ) and amlodipine group (received maleic acid levamlodipine treatment ) ,both groups were treated for 12 weeks .Blood pressure ,serum levels of tumor necrosis factor α(TNF‐α) ,interleukin‐6 (IL‐6) and high sensitive C reactive protein (hsCRP) were measured and compared between two groups before and after treatment .Results:Before treatment , there were no significant difference in blood pressure ,serum levels of TNF‐α,IL‐6 and hsCRP between two groups , P>0.05;after treatment ,all above indexes significantly reduced in both groups compared with before treatment ,P<0. 01 all .Compared with nifedipine group ,there were significant reductions in systolic blood pressure [ (136. 9 ± 13.4) mmHg vs . (128.3 ± 12.5) mmHg] ,diastolic blood pressure [ (88.4 ± 7.1) mmHg vs . (82.7 ± 6.8) mm‐Hg] ,serum levels of TNF‐α [ (10.85 ± 2.56) ng/L vs . (8.61 ± 2.37) ng/L] ,IL‐6 [ (18.92 ± 4.61) ng/L vs . (13.73 ± 4.18) ng/L] and hsCRP [ (7.95 ± 2.38) mg/L vs . (5.89 ± 2.24) mg/L] in amlodipine group , P<0.01 all .Conclusion:Amlodipine can more significantly reduce levels of TNF‐α,IL‐6 and hsCRP ,inhibit inflammatory reaction than those of nifedipine and contribute to blood pressure control in patients with essential hypertension .

Objective To analyze the biological characteristics and morphologic features between the mouse models using MDA-MB-231 cells established by injecting tumor cell suspension or implanting tumor tissues. Methods Twenty BALB/c mice were randomly divided into two groups,with 10 in each group.The orthotopic models of breast cancer were established by injection of tumor cell suspension or implantation of tumor tissues in the fat pad of right second breast in the nude mice of the two groups,respectively.The time of tumor formation,success rate,tumor growth rate,shape,central tumor necrosis,blood vessel distribution and tumor ulceration were compared between the two groups of models.The histological features of the tumors were also observed. Results There were no significant difference in the success rate between the two methods.In the group of tumor tissues implantation,the tumors formed in an earlier time with various shape configurations,and they were hypovascular and vulnerable to central necrosis with the resultant superficial ulcerations.However,in the group with injection of tumor cell suspension,the tumors formed later with a regular shape,and the superficial ulceration was rare and not vulnerable to central tumoral necrosis since the tumors were abundant in blood vessels resulting in adequate nourishment.There were no significant difference in the pathological findings between the two groups. Conclusion The nude mouse models of breast cancer can be successfully established by both tumor tissues implantation and tumor cell suspension injection,with identical biological characteristics and fairly different morphological features.The investigators are well advised to choose the more appropriate one according to the purpose of the experiments.

Objective: To provide anatomic data for management of pterygopalatine fossa lesions via endoscopic transnasal approach. Methods: Fifteen (30 sides) formalin-preserved human skulls were subjected to microsurgical dissection to evaluate the exposure scopes of 3 different endoscopic surgery approaches: endonasal middle meatal transpalatine approach, endonasal middle meatal transantral approach, and endonasal inferior turbinectomy transantral approach. Meanwhile, 20 (40 sides) dry skulls were dissected and the related measurements were obtained. Results: The 3 approaches allowed for different exposure of the pterygopalatine fossa structure. The mean distances from foramen rotundum to anterior nasal spur, superior orbital fissure, and optic canal were (61.86±3.67)mm,(3.56±0.75) mm, and (11.23±1.24) mm, respectively. Conclusion: The 3 endoscopic approaches are safe and effective for resection of different pterygopalatine fossa lesions and foramen rotundum serves as a landmark for safe management of the pterygopalatine fossa.

AIM: To observe the effect of Tanreqing Injection on patients with viral pneumonia. METHODS: 80 cases of viral pneumonia were divided into two groups randomly:40 cases of treatment group and 40 cases of contrast group. The two groups were treated with Tanreqing Injection and ribavirin differently, once per day, 10 to 14 days for one treatment period. RESULTS: The total effective rate of treatment group was superior to that of the contrast group. CONCLUSION:Tanreqing Injection is effective on treating viral pneumonia.

Ceramics ; Dental Alloys ; chemistry ; Dental Porcelain ; Dental Restoration, Permanent ; Humans ; Metal Ceramic Alloys ; chemistry

This study was aimed to explore the methodology regarding culture, proliferation and purification of adipose-derived stromal cells (ADSCs), and to study their biological characteristics. ADSCs were obtained using type I collagenase digestion method. Cell growth was observed, and cell viability were detected under different digestion period by MTT. The ADSCs were then identified and induced. The results showed that adherent cells digested by type I collagenase for 60 min had a strong proliferation capability. After the induction of different inducers these adherent cells could differentiate into nerve cells and fat cells. The best digestion period was proved to be of 60 minutes in the experiment. The results indicate that stem cells with multilineage differentiation ability could be separated from adipose tissue, namely ADSCs.
Adipocytes ; cytology ; Adipose Tissue ; cytology ; Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Neurons ; cytology ; Primary Cell Culture ; Stem Cells ; cytology ; Stromal Cells ; cytology

Objective To investigate the features of transcatheter selective hepatic arteriography and efficacy of embolization in hepatic hemangioma. Methods Twenty-six patients of hepatic hemangioma underwent transcatheter selective hepatic arteriography and embolization with Lipiodol-ultrafluida as well as Pingyangmycin, and were followed-up for 3 - 12 months after treatment. Results The popcorn-like tumor stain sign and early fill-in and persistant staining sign of hepatic arterial angiography were observed in all patients before embolization. After one time of embolization, the tumor size decreased more than 25% in 22 patients (84.62%), less than 25% in 4 patients. No severe complication occurred. Conclusion Transcatheter selective hepatic arteriography and embolization is a favorable method of diagnosis and treatment for hepatic hemangioma.

Objective To improve the quality of thyroid surgery though the special surgical instruments and operating skill.Method Sub-total thyroidectomy for 504 pafients with primary hyperthyroidism were done through MPBS illuminating instruments and dissection,scrape absorption and electric coagulation skills of PMOD.Results Five hundred and four eases of sub-total thyroidectomy were successfully performed.The operation time was(50±20)minutes.The blood loss volume Was(60±30)ml.The hospital duration was(54±1)days.The incisions were short and the sears wore even.There was 1 case recurrence of hyperthyroidism and 2 cases of hypothyroidism after(5±3)years follow-up in 482 patients.There were no postoperative complications of hypoparathyroidism and injury of nerve.Conclusion The MPBS and PMOD instruments can be safely applied in thyroid surgery with the advantage of less blood loss,shorter operation time,smaller incision and less side-injuries.

Objective To evaluate the consistency and diagnosis importance among three assays of Micral-Test strip,DCA 2000 analyzer and radioimmunoassay.Methods A total of 133 random urine samples of patients with diabetes were determined positive or questionable positive by Micral-Test strip,from whom urine samples within 8 hours during the nighttime were also collected.Both the two urine samples were determined by three assays for detection of microalbuminuria.Results(1)The value of Alb and AER determined by radioimmunoassy was significantly lower than that by DCA2000 analyzer,of which the sensitivity and specificity is 100%and 86.2%,?=0.812.(2)According to the reading scales of Micral-Test strip,the area under ROC curve(AUC)of random and 8 h urine samples were 0.900 and 0.934 respectively.(3)The AUC of random ACR and Alb was 0.952 and 0.923.The AUC of 8h urine ACR and Alb were 0.965 and 0.958.Conclusion The diagnosis importance among three assays is all dependable.Micral-Test strip is convenient for screening.ACR cannot substitute for AER as a diagnosis standard.And the value determined by DCA 2000 analyzer is more precise than that of radioimmunoassay.