OBJECTIVE: To explore the surgical ways of reoperation of the UPPP. METHOD: Fifteen failed cases of OSAHS after UPPP were reviewed and analyzed. The reoperations were performed with modified laser assistant, ablation assistant UPPP combining ablation of tongue base. The follow-up was done after 1 year. RESULT: The AHI decreased from (38.56 +/- 11.23)/h to (4.87 +/- 1.59)/h and the lowest SpO2 increased from (61.5 +/- 8.5)% to (79.9 +/- 9.7)% of the patients. The statistical difference was significant (P<0.01) in 1 year after the reoperation. The total validity ratio was 100% and the recovery rate was 96%. No complications happened. CONCLUSION The shortcomings of UPPP could be made up with ablation, and the ablation could help the UPPP more minimal damage and effective therapy.
Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Palate, Soft ; surgery ; Pharynx ; surgery ; Reoperation ; Retrospective Studies ; Sleep Apnea, Obstructive ; surgery ; Tongue ; surgery ; Treatment Failure ; Uvula ; surgery

OBJECTIVE: To investigate the feasibility and efficacy of coblation on benign hyperplasia of tongue base under endoscopy. METHOD: Fifty cases with benign hyperplasia at tongue root including tonsillar hypertrophy, lymphangioma and papillary epithelioma were treated with coblation under endoscopy and analyzed retrospectively. The post-operative Hemorrhage and pain and therapeutic effect were observed. And the follow-up was 16 to 32 months. RESULT: All the tumors were resected completely. The postoperative pain was mild. The patients could eat on the day of surgery without dysfunction of pronunciation and respiratory. The VAS score of foreign body sensation in pharynx was decreased from 81.5 +/- 9.6 to 13.5 +/- 3.9 after surgery (P < 0.01). The recovery rate was 96%, excellence rate was 4% and total effective rate was 100%. Besides, there was no recurrence. CONCLUSION Coblation is a safe, effective and minimally invasive way to treat benign hyperplasia of tongue base under endoscopy.
Adult ; Catheter Ablation ; methods ; Female ; Humans ; Hyperplasia ; Hypertrophy ; surgery ; Male ; Middle Aged ; Palatine Tonsil ; pathology ; Retrospective Studies ; Tongue Neoplasms ; pathology ; surgery ; Treatment Outcome

Objective:To evaluate the effect of donor dexmedetomidine preconditioning on the renal function of patients undergoing living-related kidney transplantation.Methods:Sixty American Society of Anesthesiologists physical status Ⅲ-Ⅳ patients, regardless of gender, aged 20-64 yr, with body mass of 18.5-28.0 kg/m 2, undergoing living-related kidney transplantation, were selected.Sixty corresponding donors, of American Society of Anesthesiologists physical status Ⅰ or Ⅱ, regardless of gender, aged 20-64 yr, with body mass index of 18.5-28.0 kg/m 2, were selected.The patients and donors were divided into 2 groups using a random number table method: control group (group C) and dexmedetomidine group (group D), with 30 pairs in each group.Before induction of anesthesia, dexmedetomidine was intravenously infused over 10 min in a loading dose of 1 μg/kg followed by an intravenous infusion of 0.5 μg·kg -1·h -1 until the time point when the renal artery was blocked immediately in the donors of group D, while the equal volume of normal saline was given instead until the time point when the renal artery was blocked immediately in the donors of group C. In both groups, total intravenous anesthesia was applied in donors and recipients, Nacotrend values were maintained at 40-60 during operation, mean arterial pressure and heart rate were maintained within the normal range, and dopamine was intravenously infused when necessary.The warm ischemia time and cold ischemia time of donor kidneys were recorded in the two groups.Peripheral venous blood samples were collected from the donors immediately before renal artery occlusion and from the recipients before renal artery opening (T 0) and at 1, 12 and 24 h after renal artery opening (T 1-3) to determine the serum creatinine (Cr), urea nitrogen (BUN) and cysteine protease inhibitor C (CysC) concentrations.The intraoperative volume of fluid infused, urine volume and consumption of propofol, remifentanil and dopamine were recorded in the receptors of two groups. Results:There was no significant difference in the concentrations of Cr, BUN and CysC and warm ischemia time and cold ischemia time of kidneys in between the two groups of donors ( P>0.05). There was no significant difference in the consumption of propofol, remifentanil and dopamine, volume of fluid infused and urine volume during surgery between the two groups of recipients ( P>0.05). Compared with group C, the concentrations of Cr, BUN and CysC were significantly decreased in at T 1 in group D ( P<0.05). Conclusion:Donor dexmedetomidine preconditioning is helpful in improving the perioperative renal function of patients undergoing living-related kidney transplantation.

OBJECTIVE: To optimize DNA library construction in non-crosslinked chromatin immunoprecipitation coupled with next-generation sequencing (Native ChIP-seq) to obtain high-quality Native ChIP-seq data. METHODS: Human nasopharyngeal carcinoma HONE1 cell lysate was digested with MNase for release of the nucleosomes, and the histone-DNA complexes were immunoprecipitated with specific antibodies. The protein component in the precipitate was digested with proteinase K followed by DNA purification; the DNA library was constructed for sequence analysis. RESULTS: Compared with the conventional DNA library construction, Tn5 transposase method allowed direct enrichment of the target DNA after Tn5 fragmentation, which was simple, time-saving and more efficient. The IGV visualized map showed that the information obtained by the two library construction methods was consistent. The sequencing data obtained by the two methods revealed more signal enrichment with Tn5 transposase library construction than with the conventional approach. H3K4me3 ChIP results showed a good reproducibility after Tn5 transposase library construction with a signal-to-noise ratio above 50%. CONCLUSIONS Tn5 transposase method improves the efficiency of DNA library construction and the results of subsequent sequence analysis, and is especially suitable for detecting histone modification in the DNA to provide a better technical option for epigenetic studies.
Chromatin Immunoprecipitation ; DNA ; Gene Library ; High-Throughput Nucleotide Sequencing ; Humans ; Reproducibility of Results ; Sequence Analysis, DNA