1.Image quality of target vessel fluency assessed by multi-slice helical CT: Does effect of coronary stent materials and structures exist?
Xin FU ; Guiping WU ; Yanhong ZHANG ; Zhaoqing SUN ; Deling ZOU
Chinese Journal of Tissue Engineering Research 2009;13(52):10297-10301
BACKGROUND:Mental material has shadow intervention effects on multi-slice helical CT (MDCT).Mental wall thickness and lumens inner diameter in stent structure can significantly affect longitudinal axis imaging quality of MDCT target vessel stent.However.there are few studies involved in this aspect.OBJECTIVE:To analyze the effect of various coronary stent materials and structures on patency images of target vessel by MDCT evaluation,and to provide clinical evidences for improving stent technique.DESIGN,TIME AND SETTING:The comparison observation was conducted at the Shenzhou Hospital,Shenyang Medical College.and Shengjing Hospital,China Medical University from January 2006 to December 2008.PARTICIPANTS:A total of 139 patients with coronary heart disease who were treated with stent implantation were divided into material and construct groups (227 stents were implanted).There were 92 stainless steel stents,135 nick-eltitanium alloy stents,85 thin mental wall stents (<140μm).142 thick mental wall stents (≥140μm).71 small diameter stents (<3 mm),and 156 big diameter stents (≥3mm).METHODS:During following up,patients were checked using 64-slice helical CT and routine coronary arteriongraphy to compare patency images of target vessels in the two groups.MAIN OUTCOME MEASURES:MDCT was scored by four-mark standard to evaluate sensitivity,specificity,accuracy,positive and negative predictive values of MDCT.RESULTS:A total of 227 stents were implanted into 139 patients.CT images of stainless steel stent group were poorer than nickel-titanium alloy group,and the indicators including image score,sensitivity,specificity,positive and negative predictive values of the stainless steel stent group were significantly less than nickel-titanium alloy group (P<0.05).CT images of thick-wall stent were poorer than thin-wall stent,while the indicators including image score,sensitivity,specificity,positive and negative predictive values of the thick-wall stent were significantly less than thin-wall stent (P<0.05).CT images of small-diameter stent were poorer than large-diameter stent,while the indicators including image score,sensitivity,specificity,positive and negative predictive values of the small-diameter stent were significantly less than large-diameter stent (P<0.05).CONCLUSION:Materials,wall thickness and diameter of coronary stent may influence images of target vessels via MDCT evaluation.
2.Changes of Membrane Fluidity and Insulin Receptor of Erythrocytes in Parkinson Disease
Cheng HE ; Xiaoping PAN ; Zhihong LU ; Binghai ZHANG ; Deling WANG ; Yonglin WU ; Weiren ZHANG
Academic Journal of Second Military Medical University 1981;0(03):-
The membrane microviscosity and insulin receptor of erythrocytes were determined by fluorescence polarization analysis and insulin radioligand binding assay simultaneously. The patients with Parkinson disease (PD) had increased membrane microviscosity (P
3.MRI signal changes in the skull base bone after endoscopic nasopharyngectomy for recurrent nasopharyngeal carcinoma
Deling WANG ; Hui LI ; Zhijun GENG ; Xuewen LIU ; Chuanmiao XIE ; Peihong WU
Chinese Journal of Radiology 2013;47(8):722-725
Objective To evaluate the signal changes of the skull base after salvage surgury via endoscopic transnasal approach for local recurrent nasopharyngeal carcinoma.Methods Twenty patients with nasopharyngeal carcinoma after radiation failure underwent nasophargeryngectomy via an endoscopic transnasal approach were selected from April 2006 to December 2011,including 16 males and 4 females with 31 to 67 years old.Each patient had previously received irradiation and experienced recurrence after 8 to 83 months of completed irradiation.All patients underwent MRI no more than 2 weeks before the salvage surgery and were subjected to repeat MRI scans 2 weeks,3 months,6 months later and semi-annually thereafter,with the follow-up time of 6 to 45 months(median 18 months).A two-sided Chi-square test was used to compare the signal changes and the tendency of changes on all presurgical and postsurgical MR images.Results The MRI signal changes were detected at 92 sites of skull-base between 2 weeks and 3 months after the surgery,which was hypointense on T1 WI with moderate to marked contrast enhancement.In the follow-up period,the signal abnormalities at 36 sites of skull base had resolved or restored to the normal,and 34 sites remained stable,while in 22 sites,the MR signal changes became more obvious.The skull base bones adjacent to the region of the resection were more likely to show signal changes than nonadjacent areas (72 vs.20,x2 =33.128,P <0.01).The signal changes were more common on the ipsilateral skull base to the recurrent tumor in contrast to the contralateral skull base (68 vs 24,x2 =21.182,P < 0.01).Conclusions The skull base signal changes after salvage surgury via endoscopic transnasal approach for local recurrent nasopharyngeal carcinoma,and it occurs in specific location.Most of sites tend to resolve or be stable at the follow up.
4.Layer-by-layer assembled selenium containing mimic artificial blood vessels with catalyzing NO generation
Yandong LI ; Kai WANG ; Meimei KONG ; Wenjie WU ; Jun AN ; Siyuan CHEN ; Yanhang HONG ; Deling KONG ; Shufang WANG
International Journal of Biomedical Engineering 2011;34(4):193-196
ObjectiveA new style of artificial vessel scaffold was designed making the use of property of organoselenium catalyzing the releasing of Nitric oxide (NO). MethodsSelenium-containing catalyst organoselenium immobilized polyethyleneimine (SePEI) as polycation and polyglutamic acid (PGA) as polyanion were alternately coated onto the surface of polycaprolactone (PCL) nanofiber scaffolds obtained by electrospinning to form the blood vessel scaffold. Self-assembly was characterized by UV and atomic absorption qualitatively and quantitatively. Catalytic generation of NO from the NO donors- RSNOs was tested under the existence of reducing agent RSH. Biological properties were also evaluated. Results The NO release was relatively stable with no significant burst appeared, and still could be detected after 80 hours of catalyzing. The material was proved to show little cytotoxicity, and displayed significant effect in inhibiting of platelet aggregation through biological testing. Conclusion The new style of artificial vessel scaffold has good effect on improving the biological properties of materials.
5.Study on industrialized production technology of fresh-cut for Paeonia radix alba.
Chuanshan JIN ; Suliang LI ; Deling WU ; Wei ZHANG
China Journal of Chinese Materia Medica 2011;36(24):3444-3448
OBJECTIVETo optimize the objective technical parameter for direct processing technology of fresh-cut for Paeonia Radix Alba.
METHODPeoniflorin and TGP were employed as the indexes of quality evaluation. Combining with the appearance of the slices, we investigated the influence of slice degree and thickness, drying temperature, drying time, drying method and other factors on the quality control of Paeonia Radix Alba slices. Furthermore, the key fresh-cut technology operating procedures parameter for Paeonia Radix Alba in Bozhou was established based on the semi-works production.
RESULTIt established tentatively the method for fresh-out and fresh-out after boiled of Paeonia Radix Alba as that selected fresh or beiled Paeonia Radix Alba, then divided them from different grades and put them into a roller to crash off the skin, cleaned and then dried them 9 h at 60 degrees C (water content 28% - 32%), and then moistened 2-3 h after spraying moderate water. Cut them into 1.5-2 mm decoction pieces and dried them at 60 degrees C, cooled, and sealed them in a package.
CONCLUSIONThe industrialization of processing decoction pieces with freshly cut Paeonia Radix Alba is stable shortens processing time, avoids effectively sulfur fumigation and infiltrating and soften, and keep high quality of production.
Drug Industry ; Paeonia ; Technology, Pharmaceutical
6.Optimization of Water Extraction Technology of Feng-Moutan Cortex by Orthogonal Test-Multiple-index Normalization Method
Pei WANG ; Hongsu ZHAO ; Deling WU ; Fengqing XU ; Wei ZHANG ; Quanjiang LONG ; Chengwu FANG
China Pharmacy 2018;29(3):361-364
OBJECTIVE: To optimize water extraction technology of Feng-Moutan Cortex. METHODS: L9 (34) orthogonal design was used to optimize soaking time, solid-liquid ratio, extraction time and extraction times in water extraction technology of Feng-Moutan Cortex using comprehensive scores for the peak area percentage and extract yield of 8 ingredients in Feng-Moutan Cortex as gallic acid, paeoniflorin, catechin, paeoniflorin, benzoic acid, benzoyloxypaeoniflorin, benzoylpaeoniflorin and paeonol after normalization as indexes. Verification test was conducted. RESULTS: The optimal water extraction technology was that 20-fold water, soaking for 1 h, extracting twice, 2 h each time. Results of verification test showed that comprehensive scores of 3 times of tests were 65. 98, 68. 85 and 69. 25, respectively. RSDs of each index were lower than 5% (n=3). CONCLUSIONS: Established comprehensive scoring method can reflect the relative contents of effective components in Feng-Moutan Cortex comprehen- sively, so that optimized extraction technology is reasonable and feasible.
7.Diffuse Intrinsic Pontine Gliomas Exhibit Cell Biological and Molecular Signatures of Fetal Hindbrain-Derived Neural Progenitor Cells.
Yu SUN ; Cheng XU ; Changcun PAN ; Xin CHEN ; Yibo GENG ; Yuliang WU ; Peng ZHANG ; Wenhao WU ; Yu WANG ; Deling LI ; Zhen WU ; Junting ZHANG ; Qiaoran XI ; Liwei ZHANG
Neuroscience Bulletin 2019;35(2):216-224
Diffuse intrinsic pontine glioma (DIPG) is the main cause of brain tumor-related death among children. Until now, there is still a lack of effective therapy with prolonged overall survival for this disease. A typical strategy for preclinical cancer research is to find out the molecular differences between tumor tissue and para-tumor normal tissue, in order to identify potential therapeutic targets. Unfortunately, it is impossible to obtain normal tissue for DIPG because of the vital functions of the pons. Here we report the human fetal hindbrain-derived neural progenitor cells (pontine progenitor cells, PPCs) as normal control cells for DIPG. The PPCs not only harbored similar cell biological and molecular signatures as DIPG glioma stem cells, but also had the potential to be immortalized by the DIPG-specific mutation H3K27M in vitro. These findings provide researchers with a candidate normal control and a potential medicine carrier for preclinical research on DIPG.
Animals
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Brain Stem Neoplasms
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genetics
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metabolism
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pathology
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Cell Line, Tumor
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Cellular Senescence
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Female
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Glioma
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genetics
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metabolism
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pathology
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Histones
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genetics
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Humans
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Mice, Inbred NOD
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Mice, SCID
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Neoplasm Transplantation
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Neoplastic Stem Cells
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drug effects
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metabolism
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pathology
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Neural Stem Cells
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drug effects
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metabolism
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pathology
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Pons
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embryology
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metabolism
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pathology
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Primary Cell Culture