Objective To establish a method for simultaneous determi-nation of 6 alkaloid in Mongolia medicine Garidi -13 pill.Methods Diamonsi I C18 column was used.The acetonitrile-tetrahydrofuran (25:15 ) and 0.1 mol? L-1 of ammonium acetate solution were used as mobile phase by gradient elution.The volume flow rate was 0.8 mL? min -1 , and column temperature was 30 ℃, wavelength was 235 nm.The speci-ficity, lower limit of quantification and standard curve , precision and re-covery rate and stability as well as the matrix effect were investigated . Results The aconitine , hypaconitine , mesaconitine , benzoylaconitine , benzoylhypacoitine and benzoylmesaconine showed a good linear relation-ship in the 3.48 ×10 -3 -0.17 ( r=0.999 5 ) ,3.24 ×10 -3 -0.16 ( r=0.999 6 ) ,6.96 ×10 -3 -0.35 ( r=0.999 5 ) ,2.06 ×10 -3 -0.10 ( r=0.999 9 ) , 2.02 ×10 -3 -0.20 ( r=0.999 9 ) and 6.66 ×10 -3 -0.33 ( r=0.999 9 ) mg? mL-1 .The RSD was 3.56%, 1.87%, 4.86%, 2.74% and 3.21%, 4.59%, respectively , and the average recovery rate was 101.11%, 98.07%, 97.81%, 9 9.24%, 104.55% and 104.84%.Conclusion The method is simple , accurate and reproduci-ble, which can simultaneously determinate the mono ester alkaloid and double ester alkaloid in the Mongolia medicine Garidi -13 pills.

Objective To evaluate the clinical efficacy of pioglitazone combined with glimepiride in treatment of type 2 diabetes mellitus.Methods A total of 100 patients with type 2 diabetes mellitus were ran-domly divided into treatment group( n=60) and control group( n=40).Patients in control group were treated with pioglitazone 15 mg per day be-fore breakfast.Patients in treatment group were treated with pioglitazone 15 mg, and glimepiride 1 mg per day before breakfast, if the blood glu-cose not controlled after 14 d, increase the dose to 2 mg.The blood glu-cose levels and adverse drug reactions of patients were recorded.The treatment lasted 28 d.Fasting plasma glucose, 2 h postprandial blood glucose, hemoglobin A1c(HbA1c) , fasting insulin and body mass index were determined and compared before and after treatment.Results The HbA1c, fasting glucose and fasting insulin were lower than those before treatment(P<0.05).The fasting insulin ,fasting glucose levels of treat-ment group were obviously lower than those of control group( P<0.05).Conclusion There was a good therapeutic effect for type 2 diabetes mellitus with pioglitazone combined with glimepiride, which can control blood sugar of patients fastly, safely and effectively.

Objective To observe the safety of Menggenwusu -18 pill on psoriasis patients.Methods Thirty -six cases of psoriasis patients were given Menggenwusu -18 pills, 9 to 11 pills once a day at a night for 28 days.The vital signs, adverse drug reactions, urine and blood rou-tine, ECG and blood biochemical parameters were recorded before and after the experiment.Results Thirty -six patients had no significant adverse reactions and the data of vital signs ,ECG,urine and blood rou-tine, liver and kidney function all were normal.Conclusion Menggen-wusu -18 pill is well tolerated by psoriasis patients.Clinical prescribed dose at range of 9 to 11 pills is recommended.

Objective To establish a HPLC method for the determina-tion of aconitine, hypaconitine, mesaconitine, benzoylaconitine, benzoylhypacoitine and benzoylmesaconine in traditional Mongolia medi-cine Menggenwusen -Wurile. Methods Column was the Halo C18 HPLC column, mobile phase consisted of acetonitrile -tetrahydrofuran (25∶15)as mobile phase A and 0.1 mol? L-1 of ammonium acetate so-lution as mobile phase B with gradient elution, the volume flow rate was 0.8 mL ? min-1 , column temperature was 30 ℃, detection wavelength was 235 nm.Results The concentration ranges of aconitine, hypaconi-tine, mesaconitine, benzoylaconitine, benzoylhypacoitine and benzoylmesaconine showed good linear relationship in the 3.48 ×10-3 -1.74 ×10-1 , 3.24 ×10 -3 -1.62 ×10-1 , 6.96 ×10 -3 -3.48 ×10-1 , 2.06 ×10 -3-1.03 ×10 -1 ,4.02 ×10 -3 -2.02 ×10 -1 ,6.66 ×10 -3 -3.33 ×10 -1 mg? mL-1 ( r were 0.999 5,0.999 0,0.999 0,0.999 9, 0.999 9, 0.999 9, respectively ) .The average recovery rates were 95.54%, 103.18%, 102.75%, 92.29%, 99.41%, 107.8%, respec-tively.RSDs were 3.70%,1.89%,4.94%,3.67%,4.29%, 3.89%, respectively.Conclusion This method is convenient, accurate and reproducible, applicable for detection of the monoester type alkaloids and diester type alkaloids in traditional Mongolia medicine Menggenwusen-Wurile.