Objective To investigate and analyze the relationship of clinicopathological stages characteristics and surgical proce-dures of perforation of gastric cancer .Methods Data of clinicopathological stages characteristics of 50 patients with perforation of gastric cancer in this hospital were retrospectively analyzed ,and the correlation between of the different clinicopathological stages and surgical procedures was analyzed .Results Fifty patients were with clinical stage Ⅱ - Ⅳ ,there were 2 cases of stage Ⅱ ,19 ca-ses of stage Ⅲ ,29 cases of stage Ⅳ .Surgical approach were simple perforation repair ,palliative surgery ,first radical resection and second radical resection ,and the number of cases were 16 cases(6 cases of stage Ⅲ ,10 cases of stage Ⅳ) ,19 cases(6 cases of stageⅢ ,13 cases of stage Ⅳ) ,4 cases(one case of stage Ⅱ ,3 cases of stage Ⅲ) and 11 cases(4 cases of stage Ⅱ ,7 cases of stage Ⅲ) . Conclusion It indicated that making reasonable choice of surgical approach according to the clinical staging can improve patients′outcomes .

BACKGROUND: Cardiac function of patients with hypertension is impaired to various extents. How does the impairment level of cardiac function of patients with hypertension differ at various stages?OBJECTIVE: To observe cardiac function of patients with hypertension at various stages and compare the indexes with those of healthy people.DESIGN: Case-control and comparative observation.SETTING: Department of Special Treatment, General Hospital of Shenyang Military Area Command of the Chinese PLA.PARTICIPANTS: Totally 100 patients with hypertension were selected from the Internal Medicine Department of General Hospital of Shenyang Military Area Command of the Chinese PLA between October 2002 and May 2003. Among them, 30 cases of hypertension were at stage Ⅰ , 40 at stage Ⅱ and 30 at stage Ⅲ. At the same time period another 30 healthy people were selected as the control group. All the subjects signed the informed consent.METHODS: Cardiac function of the included subjects were detected with XJS-Ⅰ cardiac function testing equipment. Patients were tested at supine position and electrode was put on the basis of four-probe method.Breath was held at the end of expiration; meanwhile, lead electrocardiograph Ⅰ, cardiophonogram, cardiac admittance differential coefficient method and beating of carotid artery were recorded. Various indexes were processed with the computer. Detected indexes were heart rate, ejection prophase, ejection time of left ventricle, ejection prophase/ejection time of left ventricle, delay time of electro-machine, isovolumetric contraction time, isovolumetric contraction time/ejection time of left ventricle, time of pulse wave transmission, stroke volume, cardiac output, cardiac index,stress index of myocardial contraction, total peripheral resistance, and vascular compliance.MAIN OUTCOME MEASURES: Comparison of cardiac function between hypertensive patients at various stages and healthy people.RESULTS: All the 100 patients with primary hypertension and 30healthy people entered the final analysis. [1] Ejection prophase, isovolumetric contraction time, peripheral resistance, ejection prophase/ejection time of left ventricle and isovolumetric contraction time/ejection time of left ventricle: Those of hypertensive patients were obviously longer or stronger than those of the healthy subjects (P ＜ 0.01); those of patients with hypertension at stages Ⅱ and Ⅲ were obviously longer or stronger than those of patients with hypertension at stage Ⅰ (P ＜ 0.01). [2] Stroke volume, cardiac output, stress index of myocardial contraction, vascular compliance, time of pulse wave transmission and cardiac index: Those of hypertensive patients were obviously shorter or weaker than those of the healthy subjects (P ＜ 0.01); those of patients with hypertension at stages Ⅱ and Ⅲ were obviously shorter or weaker than those of patients with hypertension at stage Ⅰ (P ＜ 0.01). Among the indexes mentioned above, vascular total peripheral resistance of hypertensive patients at stage Ⅲ was obviously stronger than that of hypertensive patients at stage Ⅱ (P ＜ 0.05),but vascular compliance of the latter ones was obviously than that of the former ones (P ＜ 0.05).CONCLUSION: Cardiac function of patients with hypertension is decreased as compared with that of healthy people, and tends to further decrease with the development of hypertensive condition.

Objective:To analyze the literature on clinical and experimental studies of sports fatigues treated and prevented by acupuncture therapy and understand the current statuS about acupuncture therapy for eliminating sports fatigue.Method:By the method to analyze the literature,the literature on treatment of sports fatigue in the recent 20 years was summarized and analyzed.Results and Conclusion:Acupuncture therapy is able to eliminate free radicals,resist lipid peroxidation,prevent dysfunction of the motor hypothalamus-pituitary-gonadal axis,reduce the creatine kinase content,and lower lactic acid concentration,so as to play an important role in the elimination of sports fatigue and improvement of athletic ability of the body.But,traditional acupuncture therapy will cause minor injury to the human body,it is necessary to popularize the painless acupuncture therapy,and explore the functional mechanism of acupuncture therapy in the treatment and prevention of sport fatigue by multiple channels in the future.

Objective To study the protective effects of choline on myocardial ischemia rat heart and its potential mecha -nisms .Methods Ischemia hypoxia environment was simulated with low value of pH (pH 6 .8) and lack of oxygen .Calcium currents were recorded by whole cell patch under the voltage clamp configuration .The alternations in[Ca2 + ]induced by KCl was detected by laser scanning confocal microscope in ventricular myocytes ,then disccuss the effects of choline on calcium and calcium store in cells . Results The normalized peak currents of ICa-L in ventricular myocytes were larger in pH 6 .8 group than those in pH 7 .4 group , which can be attenuated by choline .The(Ca2 + )i induced by KCl in ventricular myocytes were significantly increased in pH 6 .8 Ty-rode solution and its increasing can be suppressed by choline .4-DAMP can inhibit the suppressing effect of choline .Conclusion The possible mechanism of M 3 receptor involved in the protection of ischemic myocardium by inhibiting myocardial cells in ICa-L ,in-hibiting intracellular calcium overload .

Objective To investigate therapy effect and its clinical complications of the clavicular hook plate internal fixation for the treatment of Type Neer Ⅱ distal clavicle fracture and tossy Ⅲ acromioclavicular joint dislocation.Methods Twenty-two patients of Neer Ⅱ distal clavicle fracture and 16 patients of Tossy Ⅲ acromioclavicular joint dislocation were selected as our subjects.All patients were hospitalized and treated by using clavicular hook plate fixation form August 2007 to Februay 2012.The clinical effect and complications were analyzed retrospectively.Results All patients were showed the primary healing in terms of incision.Of all patients,30 cases were being following up,and follow-up periods was 6.0 to 18.0 months (average of (10.45 ±3.78) months).All patients underwent implant removal surgery at postoperative 6-14 months.No refracture or acromioclavicular joint separation again occurred.Four patients were with complications,of which,2 patients were clavicular hook plate decoupling at 7 days or 3 weeks after operation; One patient occurred clavicle stress fracture at more than 3 months after operation.All mentioned 3 cases were treated with re-operation.1 patient was with shoulder abnormal sound after operation and abnormal sound disappear after internal fixation removed.The shoulder functions were evaluated according to University of California-Los Angeles (UCLA) score system at before and after clavicular hook plate was taken out.The scores of pain and function of the shoulder,forward lateral flexion range were significant difference between before and after clavicular hook plate removed ((7.90 ±1.20) vs.(9.20±1.03),t =-2.327,P=0.045;(8.00±0.94) vs.(9.40 ±0.97),t=-3.280,P=0.001 ; (4.00 ±0.47) vs.(4.70 ± 0.48),t =-4.583,P =0.001).After the hook plate was removed,all shoulder functions showed improvement.The overall efficacy was excellent in 15 cases,good in 13 cases,and poor in 2 cases,and the recovery of excellent and good rate were 93.33％.Conclusion The clinical efficacy is well in terms of using clavicular hook plate internal fixation to treat Type Neer Ⅱ distal clavicle fracture and Tossy Ⅲ acromioclavicular joint dislocation.Prevention and treatment of complications should paid more attention.

AIM To evaluate the integration method for analysis of voltage-dependent Ca2+-independent transient outward K+ currents (Ito) in pharmacology. METHODSThe inactivation phases of Ito were best fitted by the sum of two or three exponentials equations. The area under the raw current curves (AUC) was obtained by the integration of exponential equations. The AUC normalized to the cell capacitance represented the net K+ charge flow during any depolarized duration and was as the index for comparison. Calcineurin overexpression transgenic (TG) mice showed downregulation of Ito. These data were tested by the integration method. RESULTS AUC obtained from three or two exponentials fittings was calculated as: AUC=A1τ1+A2τ2+A3τ3+A0t-A1τ1e-t/τ1-A2τ2e-t/τ2-A3τ3e-t/τ3 or AUC=A1τ1+A2τ2+A0t-A1τ1e-t/τ1-A2τ2e-t/τ2. The 50% and 90% action potential duration (APD50, APD90) in ventricular myocytes of mice are about 10 ms and 30 ms, respectively. AUC at 10 ms (AUC50, AUC of 50% APD) and 30 ms (AUC90, AUC of 90% APD) in left ventricle cardiomyocytes of wild type (WT) and TG mice were normalized to the cell capacitance. The normalized AUC50 and AUC90 of WT group were significantly more than those of TG group, which was consistent to the prolongation of APD in TG mice and the previous published results(downregulation of components of Ito in TG mice). CONCLUSION The integration method was an ideal way for analysis of transient outward K+ currents in pharmacology.

Objective To study the clinical characteristics and to summarize our experience in the diagnosis and treatment of ectopic splenic autotransplantation (ESAP) many years after splenic rupture and splenectomy.Methods The clinical data of 15 patients with ESAP who were treated in the People's Hospital of Zhengzhou from January 1998 to January 2013 were retrospectively analyzed.Results Of the 15 patients,2 patients presented with intestinal obstruction due to the ectopic splenic tissues and required partial resection of small intestine.In 5 patients,the ectopic splenic tissues were in the greater omentum which required resection of part or the whole of the greater omentum.In 4 patients,the ectopic splenic tissues were in the mesentery which required partial or total resection of the tissues.The ectopic tissues were in the pelvis in 3 patients and resection of the left fallopian tube was carried out in 2 patients and resection of the pelvic tissues in 1 patient.In 1 patient the diagnosis was made under laparoscopic biopsy,thus avoiding open surgery.Three patients had multiple implanted sites for these ectopic splenic tissues.Conclusions ESAP is an uncommon disease and it has no clinical characteristics.It is easy to confuse the condition with metastatic deposits.A diagnosis could be made only by combining the history with magnetic resonance imaging,or 99mTc study of red blood cells,or laparoscopic exploration and biopsy.

Objective To observe the clinical efficacy of electroacupuncture at cervical Jiaji (EX-B2) points plus behavioral intervention in treating cervical spondylosis. Method The cervical spondylosis patients were randomized into two groups at a ratio of 3:1, 90 cases in the electroacupuncture group and 30 cases in the medication group. The patients who received electroacupuncture were also given cupping and behavioral intervention (raising head for 1 min every 20-30 min and correcting sleep habits). The clinical efficacy was evaluated according to the symptoms and body signs assessment scale. Result Respectively after 4-week, 8-week, 4-month and 6-month treatments, the clinical control rate, markedly control rate and total effective rate in the electroacupuncture group were significantly higher than that in the medication group. Conclusion Electroacupuncture at Jiaji points plus behavioral intervention is an effective solution to prevent and treat cervical spondylosis.

Objective: To analyze the trend of lung cancer incidence rate among rural residents in Feicheng city between the years 2000 and 2012,and predict the incidence rate between the years 2013 and 2018,and subsequently provide baseline data for lung can-cer control and prevention.Methods:With the cancer registration data in Feicheng rural areas,the time trend of lung cancer inci-dence rate and the annual percentage change(APC)were calculated by the Joinpoint model,while the incidence of lung cancer from 2013 to 2018 were predicted by the ARIMA(p,d,q)model.Results:A total of 3,908 new cases of lung cancer were diagnosed be-tween 2000 to 2012.The incidence rate was 40.77/105,the age-specific cancer incidence rate in the Chinese population(ASRC)and world population(ASRW)were 32.95/105 and 32.97/105,respectively.The incidence was 2.14 times higher among males than females (P<0.001).The incidence of lung cancer which apparently rose from 25.13/105to 64.92/105 with an APC value of 9.74%(P<0.001),was increasing every year.The change in the trend of lung cancer with respect to age could be divided into three segments,the incidence rate in the 0 to 59 years group showed a rapid upward trend(APC=113.38,P<0.001),which was lower in the 60 to 79 years group (APC=20.39,P<0.05)and began to decline in the 80 years or older group(APC=-21.20,P>0.05).The incidence of lung cancer was also observed to be increasing yearly from 2013 to 2018,and with an average annual growth rate of 4.92%,was predicted to reach 87.92/105 in 2018.Conclusions:The occurrence of lung cancer was closely related to population aging,unhealthy habits,and environmental risk factors.Due to the increasing aging population,the incidence of lung cancer will continue to increase.In order to formulate specif-ic strategies,the control and prevention of lung cancer must be based on its incidence features.

Our previous work found that DMH1 (4-[6-(4-isopropoxyphenyl)pyrazolo [1,5-a]pyrimidin-3-yl]quinoline) was a novel autophagy inhibitor. Here, we aimed to investigate the effects of DMH1 on chemotherapeutic drug-induced autophagy as well as the efficacy of chemotherapeutic drugs in different cancer cells. We found that DMH1 inhibited tamoxifen- and cispcis-diaminedichloroplatinum (II) (CDDP)-induced autophagy responses in MCF-7 and HeLa cells, and potentiated the anti-tumor activity of tamoxifen and CDDP for both cells. DMH1 inhibited 5-fluorouracil (5-FU)-induced autophagy responses in MCF-7 and HeLa cells, but did not affect the anti-tumor activity of 5-FU for these two cell lines. DMH1 itself did not induce cell death in MCF-7 and HeLa cells, but inhibited the proliferation of these cells. In conclusion, DMH1 inhibits chemotherapeutic drug-induced autophagy response and the enhancement of efficacy of chemotherapeutic drugs by DMH1 is dependent on the cell sensitivity to drugs.