Humans ; Male ; Middle Aged ; Tuberculoma ; Tuberculosis, Hepatic

ObjectiveTo investigate the effect of erythropoietin (EPO) on the mRNA expression of apoptosis genes bcl-2 and bax in rats with fatty liver disease after hepatic ischemia-reperfusion injury. MethodsA total of 100 male adult Sprague-Dawley rats were fed with high-fat diet for 12 weeks. After the model was established, the rats with fatty liver disease were randomly divided into sham-operation (SHAM) group, ischemia/reperfusion (IR) group, IR+low-dose EPO group (EPO-1 group), IR+middle-dose EPO group (EPO-2 group), and IR+high-dose EPO group (EPO-3 group). The IR model was established by completely blocking the blood flow in the middle and left lobes of the liver to induce ischemia in 70% of the liver, with an ischemia time of 80 minutes. The rats were sacrificed at 1, 3, and 6 hours of reperfusion after 80 minutes of ischemia, and RT-PCR was used to measure the mRNA expression of bcl-2 and bax in liver tissue. A one-way analysis of variance was used for comparison of continuous data between multiple groups, and the least significant difference test was used for further comparison between two groups. ResultsCompared with the IR group, the EPO-1, EPO-2, and EPO-3 groups had a significant increase in the mRNA expression of bcl-2 and a significant reduction in the mRNA expression of bax at each time point (all P＜0.05). The SHAM group and the EPO groups had a significantly higher bcl/bax mRNA ratio than the IR group at each time point (all P＜0.05). ConclusionEPO exerts a protective effect against hepatic ischemia-reperfusion injury in rats with fatty liver disease, possibly by promoting the mRNA expression of bcl-2 and inhibiting the mRNA expression of bax. High-dose EPO has a better effect than middle- and low-dose EPO.

Adenoma, Liver Cell ; Gallbladder Diseases ; Histiocytosis, Sinus ; Humans ; Liver Neoplasms

Objective To understand the prevalence status quo of mild cognitive impairment(MCI)in the elderly people by conducting the investigation on the elderly people in somewhere,and to explore the influ-encing factors of MCI in the elderly people to provide reference for constructing the MCI localized computer screening and diagnosis system in the elderly.Methods A multi-stage sampling method was used to deter-mine the survey population.The self-made questionnaires,mini mental state examination(MMSE),geriatric depression screening scale(GDS-15),geriatric anxiety screening scale,geriatric psychiatric symptoms scale,and daily living ability scale were used to collect or evaluate basic information,cognitive impairment,depres-sion,anxiety,psychiatric symptoms,and daily living ability of elderly people from March 5,2022 to July 22,2022.The index system affecting MCI was screened by the receiver operating characteristic(ROC)curve and unconditional binary logistic regression,and the localized computer screening diagnosis system was construc-ted.Results A total of 1 004 elderly individuals were surveyed,among them there were 262 cases(26.1％)of MCI,194 cases(19.3％)of anxious state,21 cases(2.1％)of depression,313 cases(31.2％)of psychotic symptoms and 32 cases(3.2％)of impaired ability of activities of daily living(ADL).The ROC curve analysis showed that the area under the curves(AUC)of depression,anxiety and psychotic symptoms for predicting MCI in elderly individuals were 0.640,0.645,and 0.586,respectively.AUC of anxiety combined with depres-sion(anxiety+depression),depression+psychotic symptoms,anxiety+psychotic symptoms and depression+anxiety+psychotic symptoms were 0.676,0.652,0.645 and 0.676,respectively.The multivariate analysis found that the age,whether or not anyone close to you died in the last two years,ability of activities of daily living,anxiety degree,whether or not having psychotic symptoms,education level,whether or not having joint habitation with the family members and suffering from multiple chronic diseases were the influencing factors of MCI.Conclusion The age,education level,death of close persons in the past two years,ability to live daily life,depression,and psychotic symptoms could serve as the indicators system of the MCI localized computer screening and diagnosis system.