Objective To evaluate the effects of alcohol extract of juglans mandshurica maxim (AEBJ) on immune function of ionizing irradiated mice.Methods The mice were randomly divided into normal control group,irradiating control group,low AEBJ(300 mg?kg-1)irradiating group,high AEBJ(600 mg? kg-1)irradiating group,low AEBJ plus drugs only group and high AEBJ plus drugs only group.The number of WBC and LYMPH,LYMPH%,viscera index,ability of lymphocytes transformation were examined.Results Compared with normal control group,the number of WBC and LYMPH,LYMPH%,viscera index,ability of lymphocytes transformation in irradiating control group were decreased significantly (P

OBJECTIVE: To observe the effects of psoralen plus ultraviolet-A light (PUVA) on K562 cells and the relative mechanism. METHODS: The effects of psoralen, ultraviolet-A light and PUVA on K562 cells were assayed by monotetrazolium test (MTT). DNA content was analyzed by flow cytometry (FCM). The apoptotic rates of K562 cells treated with 40 and 80 microg/ml psoralen for 24 and 48 hours were assayed by Annexin-V-FITC/PI reagent kit on FCM respectively. The ultrastructures of apoptotic cells were observed by a transmission electron microscope (TEM). RESULTS: Either single psoralen therapy or single ultraviolet-A irradiation had inhibiting effect on K562 cells. The inhibiting effect of PUVA on K562 cells was stronger than that of the single psoralen therapy or single ultraviolet-A light irradiation (P<0.05). Apoptotic peak (AP) was detected by FCM. TEM test showed that K562 cells treated with PUVA were smaller, having condensed cell nucleus, assembled chromatin, disintegrated nucleus body and the majority of the cells appeared to be apoptotic conformation. CONCLUSION: Psoralen has inhibiting effect on K562 cells, and the effect of PUVA is more significant. It is suggested that 10 min irradiation and 40 microg/ml terminal concentration of psoralen be probably the best choice for PUVA. The inhibiting effect of PUVA is due to apoptosis.

Objective: To investigate the incidence trend of tuberculosis in Haidong City, Qinghai Province from 2006 to 2020, and the effects of age, period, and cohort on tuberculosis incidence, so as to provide the basis for enhancing the prevention and control for tuberculosis. Methods: Data of tuberculosis cases in Haidong City from 2006 to 2020 were collected from the Chinese Disease Prevention and Control Information System. Incidence rates were calculated and standardized using data from the Sixth National Population Census in 2010. The trends in incidence of tuberculosis were analyzed by annual percent change (APC). Effects of age, period and cohort on the incidence of tuberculosis were analyzed by an age-period-cohort model. Results: The crude incidence rates of tuberculosis in Haidong City from 2006 to 2020 ranged from 37.69/105 to 100.93/105, and the standardized incidence rates ranged from 42.85/105 to 115.24/105. The standardized incidence rates from 2006 to 2015 showed a decreasing trend (APC=-7.148%, P<0.05), while there was no significant trend observed from 2015 to 2020 (all P>0.05). The age-period-cohort model analysis showed that the highest incidence risk of tuberculosis in Haidong City from 2006 to 2020 was observed in the age group of 20-<25 years (RR=2.973, 95%CI: 2.353-3.756), followed by the age group of 80-<85 years (RR=2.785, 95%CI: 2.206-3.516). The incidence risk of tuberculosis was higher in the period from 2016 to 2020 (RR=1.253, 95%CI: 1.203-1.306) compared to the period from 2011 to 2015 (RR=0.796, 95%CI: 0.770-0.823). Tuberculosis incidence risk was the highest in the birth cohort from 1936 to 1940 (RR=3.050, 95%CI: 2.356-3.949), and then gradually decreased over time thereafter. Conclusions The incidence of tuberculosis in Haidong City showed a decreasing trend from 2006 to 2015, while there was no significant trend observed from 2015 to 2020. The incidence risk of tuberculosis was higher in the age groups of 20-<25 years and 80-<85 years, and the risk decreased for those born in more recent years.

Objective:To investigate the expression levels and diagnostic value of platelet parameters, fibrinogen and D-dimer in different Traditional Chinese Medicine (TCM) syndromes of deep venous thrombosis (DVT).Methods:From June 2015 to June 2019, a total of 500 DVT patients were enrolled and collected by two attending TCM doctors and classified according to syndromes differentiation. The differences of platelet count (PLT), mean platelet volume (MPV), platelet distribution width (PDW), platelet crit (PCT), platelet larger cell ratio (P-LCR), fibrinogen (FIB) and D-dimer were detected and compared.Results:According to TCM syndromes differentiation, 500 patients were divided into three groups, including 286 cases (57.2%) of downward flowing of damp-heat group, 132 cases (26.4%) of blood stasis and heavy damp group, and 82 cases (16.4%) of spleen-kidney-yang deficiency group. The levels of MPV, PDW, P-LCR, FIB and D-dimer among the groups were statistically significant difference ( P<0.05 or P<0.01). The MPV level was significantly higher and D-dimer level was significantly lower in blood stasis and heavy damp group than in the downward flowing of damp-heat group ( P<0.05). The levels of MPV, PDW and P-LCR in spleen-kidney-yang deficiency group were significantly higher than those in downward flowing of damp-heat group and blood stasis and heavy damp group ( P<0.05 or P<0.01). The levels of FIB and D-dimer were significantly lower than those in the downward flowing of damp-heat group and blood stasis and heavy damp group ( P<0.05 or P<0.01). The FIB (AUC=0.593) and D-Dimer (AUC=0.673) were statistically significant in the differential diagnosis of DVT between blood stasis and heavy damp group and spleen-kidney-yang deficiency group ( P<0.01). The MPV (AUC=0.601 5), PDW (AUC=0.615 4), P-LCR (AUC=0.606 1), FIB (AUC=0.616 4) and D-Dimer (AUC=0.721 8) were statistically significant in the differential diagnosis of DVT between downward flowing of damp-heat group and spleen-kidney-yang deficiency group ( P<0.01). Conclusion:The MPV, PDW, P-LCR, FIB and D-dimer have a certain correlation with DVT TCM syndrome types, and also have a certain reference value for its differential diagnosis, which can be used as an effective supplement to the objective indicators of Micro syndrome differentiation of traditional Chinese medicine.

Neurodevelopmental disorders(NDDs)are chronic developmental brain disorders that can affect cognition,motor,social adaptation,behavior and so on due to multiple genetic or acquired causes.Natural polysaccharides are synthesized by living organisms,located in the cell wall,inside and between cells,and outside the cells,and are essential components of life activities.Previous studies have found that natural polysaccharides play an important role in neurological diseases,which mainly ameliorate the behavioral abnormalities and clinical symptoms caused by anti-oxidative stress,anti-neuronal apoptosis,anti-neuroinflammation,anti-excitatory amino acid toxicity,and regulation of the brain-gut axis.This review summarizes the intervention role of 17 bioactive polysaccharides from plants and fungi in neurological diseases,aiming to provide new ideas for the research and treatment of NDDs.

Inflammatory bowel disease （IBD）， mainly including ulcerative colitis （UC） and Crohn's disease （CD）， is a common chronic inflammatory disease of the gastrointestinal tract， with its incidence increasing year by year. Due to its long treatment duration， difficulty in treatment， prolonged remission， and high costs， it has attracted global attention. Exploring safe， effective， and sustainable treatment regimens has become an urgent global issue. The pathogenesis of IBD is complex， involving intestinal mucosal injury，disturbances in the internal environment， and inflammatory responses. In recent years， research has found that ferroptosis is also one of the important pathogenic factors of IBD. Ferroptosis， as a new form of non-apoptotic cell death， is characterized by iron dependence， lipid peroxidation， and imbalance in the redox system. Studies have shown that inhibiting ferroptosis in intestinal epithelial cells can protect the intestinal mucosa. Targeted intervention in ferroptosis may be a new direction for the treatment of IBD. IBD is mainly treated with drugs， including corticosteroids， aminosalicylates， biologics， and immunomodulators， but drug resistance and adverse reactions are common. Traditional Chinese medicine （TCM） has unique advantages such as low cost， low drug resistance， and fewer side effects， and has accumulated rich experience in the treatment of IBD. Scholars have confirmed that TCM can inhibit ferroptosis， and recent studies have shown that TCM can not only inhibit iron-dependent lipid peroxidation in intestinal cells but also enhance the antioxidant and anti-inflammatory abilities of intestinal mucosa， thus playing a role in the treatment of IBD. Increasing evidence suggests that TCM may treat IBD by interfering with ferroptosis. This article explores the relevance of TCM intervention in ferroptosis and the treatment of IBD， discusses the possible mechanisms of ferroptosis in IBD， and aims to provide a basis for the diagnosis and treatment of IBD.