The independent component analysis is a new method for signal processing, which has been paid attention to in the field of biomedical engineering. This paper introduces the design of biopotential analysis system based on ICA neural network and discusses its foundamental components.

OBJECTIVE: To explore the effects of psoralen (PSO) plus long-wave ultraviolet-A (PUVA) on apoptosis and expression of Fas ligand (FasL) in HL-60 leukemia cells. METHODS: The HL-60 cells were taken as the study objects and their apoptosis rates, ultrastructure changes and the expression of FasL were detected in order to observe the effects of PSO and ultraviolet-A (UVA) of wave length 360 nm. The factorial design and analysis of variance were used to analyze the interaction among the factors. RESULTS: PSO, UVA and PUVA all induced the apoptosis and the effects of PUVA were stronger than those of the other two. After HL-60 cells had been treated with PUVA, they all showed obvious ultrastructure changes due to apoptosis observed under the electron microscope. PSO, UVA and PUVA all decreased the expressions of FasL gene and protein. The effects of PUVA were stronger than those of the other two. CONCLUSIONS: PUVA can induce the apoptosis of HL-60 cells and the effects are stronger than those of PSO or UVA alone. The expression of FasL gene in HL-60 cells is down-regulated during the apoptosis induced by PUVA.

With the modernization of the medical records and the planning implementation of informationalized strategy,the management of medical records has been extended to the stage of supervising resources electronic and digital,also the informationalized features is more evident.one reason is the integration and storage of medical records information need informationalized technology support,and the other is the socialization of utilizing medical records needs the internet technology as platform,and the third is the exploration and utilization of resources in medical records requires advanced data warehouse and data mining technology as guaranty,at the same time,the management of medical records information have the man-made risk of divulging secrets,the risk of damage to the integrity,the risk may be to deny the fact,as well as the risk of information systems security.The corresponding preventive measures taken to standardize the medical records management processes,improve its management system and the law,will be a long-term and arduous task to the quality manager.

The nonlinear wavelet method is used to detect ECG signal. The ECG signal disrupted by high-frequency noise is decomposed with a dyadic Daubechies wavelet according to Mallat algorithm. The principle of the nonlinear wavelet detection method is discussed. Along with the Lipschitz criterion, it is applied to denoising the disrupted ECG signal. The ECG signal is effectively recovered while denoised.

To study the effects of psoralen (PSO) with long wave ultraviolet light (PUVA) on apoptosis and mitochondrial membrane potential in K562 cells.

Objective To explore the effects of psoralen(PSO)and long-wave ultraviolet chemical therapy(PUVA) on cell apoptosis and expressions of FasL in HL-60,K562,and NB4 leukemia cells.Methods The cells were taken as the studying objects and their apoptosis ratios,ultrastructure changes and the expressions of FasL were detected in or- der to observe the effects of PSO extracted from Chinese medicine and ultraviolet at 360 nm on human leukemia cells.The factor design and analysis of variance were used to analyze the interaction among the factors.Results(1)PSO,UVA and PUVA all induced the apoptosis and the effects of PUVA were stronger than the other two.(2)Obvious changes of ultra- structure of leukemia cells were found under electron microscope after treatment with PUVA.(3)PSO,UVA and PUVA down-regulated FasL gene and protein expression levels,and the effects of PUVA are the strongest.Conclusion PUVA can induce the apoptosis of human leukemia cells and its effects are the strongest.One of the pathway of PUVA to induce apoptosis is by down-regulating the expression of FasL gene.

To explore the effects of inactivated rabbit serum containing compound realgar and natural indigo tablet (CRNIT) on cell line NB(4).

OBJECTIVE: To observe the effects of psoralen plus ultraviolet-A light (PUVA) on K562 cells and the relative mechanism. METHODS: The effects of psoralen, ultraviolet-A light and PUVA on K562 cells were assayed by monotetrazolium test (MTT). DNA content was analyzed by flow cytometry (FCM). The apoptotic rates of K562 cells treated with 40 and 80 microg/ml psoralen for 24 and 48 hours were assayed by Annexin-V-FITC/PI reagent kit on FCM respectively. The ultrastructures of apoptotic cells were observed by a transmission electron microscope (TEM). RESULTS: Either single psoralen therapy or single ultraviolet-A irradiation had inhibiting effect on K562 cells. The inhibiting effect of PUVA on K562 cells was stronger than that of the single psoralen therapy or single ultraviolet-A light irradiation (P<0.05). Apoptotic peak (AP) was detected by FCM. TEM test showed that K562 cells treated with PUVA were smaller, having condensed cell nucleus, assembled chromatin, disintegrated nucleus body and the majority of the cells appeared to be apoptotic conformation. CONCLUSION: Psoralen has inhibiting effect on K562 cells, and the effect of PUVA is more significant. It is suggested that 10 min irradiation and 40 microg/ml terminal concentration of psoralen be probably the best choice for PUVA. The inhibiting effect of PUVA is due to apoptosis.

Objective:To investigate the clinical efficacy of ginkgo biloba extract dense tablets combined with pitavastatin in the treatment of elderly stable coronary heart disease (SCAD), as well as its impact on patients′ thrombotic status, neutrophil-to-lymphocyte ratio (NLR), and serum high-sensitivity C-reactive protein (hs-CRP) levels.Methods:A prospective study was conducted on 82 elderly SCAD patients admitted to the 928th Hospital of PLA Joint Logistics Support Force from October 2019 to December 2020. The patients were divided into an observation group and a control group using a random number table method, with 41 patients in each group. The control group received oral pitavastatin calcium tablets, while the observation group received oral ginkgo biloba extract dense tablets on the basis of the control group. After 8 weeks of continuous treatment, the clinical efficacy of the two groups was observed. The frequency of angina attacks, thrombotic status indicators [D-dimer (D-D), fibrinogen (FIB)], peripheral blood NLR, serum hs-CRP levels were compared before and after treatment between the two groups. The incidence of adverse reactions was compared between the two groups.Results:The total effective rate of the observation group was 78.05%(32/41), which was significantly higher than 56.10%(23/41) of the control group, and the difference was statistically significant ( P<0.05). After treatment, the frequency of angina attacks [weekly attack times, duration of single attack, severity (weekly nitroglycerin usage)] in both groups significantly improved compared with before treatment (all P<0.05), and the observation group improved more significantly (all P<0.05). After treatment, plasma D-D and FIB levels in both groups significantly decreased compared with before treatment (all P<0.05), and the observation group decreased more significantly (all P<0.05). Peripheral blood NLR and serum hs-CRP levels in both groups significantly decreased compared with before treatment (all P<0.05), and the observation group was significantly lower than the control group (all P<0.05). There was no significant difference in the incidence of adverse reactions between the observation group and the control group [9.76%(4/41) vs 7.32%(3/41)] ( P>0.05). Conclusions:The overall efficacy of ginkgo biloba extract dense tablets combined with pitavastatin in the treatment of elderly SCAD is exact, which can effectively improve patients′ angina attacks, thrombotic status and inflammatory state with good safety.

Objective:To investigate the expression levels and diagnostic value of platelet parameters, fibrinogen and D-dimer in different Traditional Chinese Medicine (TCM) syndromes of deep venous thrombosis (DVT).Methods:From June 2015 to June 2019, a total of 500 DVT patients were enrolled and collected by two attending TCM doctors and classified according to syndromes differentiation. The differences of platelet count (PLT), mean platelet volume (MPV), platelet distribution width (PDW), platelet crit (PCT), platelet larger cell ratio (P-LCR), fibrinogen (FIB) and D-dimer were detected and compared.Results:According to TCM syndromes differentiation, 500 patients were divided into three groups, including 286 cases (57.2%) of downward flowing of damp-heat group, 132 cases (26.4%) of blood stasis and heavy damp group, and 82 cases (16.4%) of spleen-kidney-yang deficiency group. The levels of MPV, PDW, P-LCR, FIB and D-dimer among the groups were statistically significant difference ( P<0.05 or P<0.01). The MPV level was significantly higher and D-dimer level was significantly lower in blood stasis and heavy damp group than in the downward flowing of damp-heat group ( P<0.05). The levels of MPV, PDW and P-LCR in spleen-kidney-yang deficiency group were significantly higher than those in downward flowing of damp-heat group and blood stasis and heavy damp group ( P<0.05 or P<0.01). The levels of FIB and D-dimer were significantly lower than those in the downward flowing of damp-heat group and blood stasis and heavy damp group ( P<0.05 or P<0.01). The FIB (AUC=0.593) and D-Dimer (AUC=0.673) were statistically significant in the differential diagnosis of DVT between blood stasis and heavy damp group and spleen-kidney-yang deficiency group ( P<0.01). The MPV (AUC=0.601 5), PDW (AUC=0.615 4), P-LCR (AUC=0.606 1), FIB (AUC=0.616 4) and D-Dimer (AUC=0.721 8) were statistically significant in the differential diagnosis of DVT between downward flowing of damp-heat group and spleen-kidney-yang deficiency group ( P<0.01). Conclusion:The MPV, PDW, P-LCR, FIB and D-dimer have a certain correlation with DVT TCM syndrome types, and also have a certain reference value for its differential diagnosis, which can be used as an effective supplement to the objective indicators of Micro syndrome differentiation of traditional Chinese medicine.