Objective To evaluate the effects of alcohol extract of juglans mandshurica maxim (AEBJ) on immune function of ionizing irradiated mice.Methods The mice were randomly divided into normal control group,irradiating control group,low AEBJ(300 mg?kg-1)irradiating group,high AEBJ(600 mg? kg-1)irradiating group,low AEBJ plus drugs only group and high AEBJ plus drugs only group.The number of WBC and LYMPH,LYMPH%,viscera index,ability of lymphocytes transformation were examined.Results Compared with normal control group,the number of WBC and LYMPH,LYMPH%,viscera index,ability of lymphocytes transformation in irradiating control group were decreased significantly (P

OBJECTIVE: To explore the effects of psoralen (PSO) plus long-wave ultraviolet-A (PUVA) on apoptosis and expression of Fas ligand (FasL) in HL-60 leukemia cells. METHODS: The HL-60 cells were taken as the study objects and their apoptosis rates, ultrastructure changes and the expression of FasL were detected in order to observe the effects of PSO and ultraviolet-A (UVA) of wave length 360 nm. The factorial design and analysis of variance were used to analyze the interaction among the factors. RESULTS: PSO, UVA and PUVA all induced the apoptosis and the effects of PUVA were stronger than those of the other two. After HL-60 cells had been treated with PUVA, they all showed obvious ultrastructure changes due to apoptosis observed under the electron microscope. PSO, UVA and PUVA all decreased the expressions of FasL gene and protein. The effects of PUVA were stronger than those of the other two. CONCLUSIONS: PUVA can induce the apoptosis of HL-60 cells and the effects are stronger than those of PSO or UVA alone. The expression of FasL gene in HL-60 cells is down-regulated during the apoptosis induced by PUVA.

Objective To quantify the correlation between isometric and isokinetic tests of core muscles during trunk rotation. Methods The strength of the core muscles of 38 healthy males was measured isometrically and isokinetically ( at 60°/s) during trunk rotation.The left and right peak rotation torque ( LPT,RPT) and the ratio of left to right torque (L/R) were analyzed using correlation and regression analysis and paired t-tests. Results The subjects' LPTs and RPTs were positively correlated ( rL =0.644,P ≤ 0.01 ;rR =0.566,P≤0.01 ) There were significant differences in the L/R ratios determined using the two tests (r=0.663,P≤0.01 ).The regression equations predicting LPT and RPT were yL =22.330 + 0.937x and yR =32.752 +0.847x,respectively.Paired t-tests showed that tL =4.562,P≤0.01 and tR =3.855,P≤0.01 during left or right rotation.There was a significant difference,but there was no significant difference in LPT/RPT. Conclusion Isometric and isokinetic tests of core muscles during trunk rotation give results which are strongly correlated.Either can correctly reflect the maximal strength of core muscles during trunk rotation.Clinicinas may reasonably choose either testing method to assess patients and guide treatment according to the patient's clinical symptoms and the severity of the problem.

To study the effects of psoralen (PSO) with long wave ultraviolet light (PUVA) on apoptosis and mitochondrial membrane potential in K562 cells.

Objective To explore the effects of psoralen(PSO)and long-wave ultraviolet chemical therapy(PUVA) on cell apoptosis and expressions of FasL in HL-60,K562,and NB4 leukemia cells.Methods The cells were taken as the studying objects and their apoptosis ratios,ultrastructure changes and the expressions of FasL were detected in or- der to observe the effects of PSO extracted from Chinese medicine and ultraviolet at 360 nm on human leukemia cells.The factor design and analysis of variance were used to analyze the interaction among the factors.Results(1)PSO,UVA and PUVA all induced the apoptosis and the effects of PUVA were stronger than the other two.(2)Obvious changes of ultra- structure of leukemia cells were found under electron microscope after treatment with PUVA.(3)PSO,UVA and PUVA down-regulated FasL gene and protein expression levels,and the effects of PUVA are the strongest.Conclusion PUVA can induce the apoptosis of human leukemia cells and its effects are the strongest.One of the pathway of PUVA to induce apoptosis is by down-regulating the expression of FasL gene.

Objective:To analyze the value of minute ventilation to carbon dioxide production slope (VE/VCO 2 slope) combined with peak systolic blood pressure (SBP) in predicting prognosis for patients with chronic heart failure (CHF). Methods:A total of 170 patients with CHF who visited the Cardiac Rehabilitation Center of Tongji Hospital Affiliated to Tongji University and completed cardiopulmonary exercise test from March 2007 to December 2018 were enrolled in the study. The clinical data, cardiopulmonary exercise testing results and follow-up information of patients were collected to explore the predictors of all-cause mortality in patients with CHF.Results:The median follow-up time was 647 (182-1 764) days. All-cause death occurred in 34 patients. Compared with surviving patients, the proportion of diabetes and angiotensin-converting enzyme inhibitor/angiotensin Ⅱ receptor blocker (ACEI/ARB) use in fatal patients was significantly higher ( P<0.01). The VE/VCO 2 slope and peak SBP*VE/VCO 2 in the fatal patients were significantly higher, and the peak oxygen consumption (peak VO 2) was lower than those in the surviving patients ( P<0.01). The areas under the receiver operating characteristic curve (AUC) of VE/VCO 2 slope and peak SBP*VE/VCO 2 in predicting all-cause mortality in patients with CHF were 0.648 ( P=0.008) and 0.681 ( P=0.001), respectively; the optimal thresholds were >40.95 ( P=0.008) and > 5 423.50 mmHg (1 mmHg=0.133 kPa, P=0.006), the sensitivity was 0.559 and 0.588, and the specificity was 0.728 and 0.735, respectively. Multivariate Cox regression analysis showed that after adjusting for age, gender, diabetes and ACEI/ARB use, VE/VCO 2 slope ( HR=2.12, P=0.036) and peak SBP*VE/VCO 2 ( HR=2.42, P=0.016) were independent risk factors for all-cause mortality in patients with CHF. Conclusion:Compared to the traditional index VE/VCO 2 slope, a novel index peak SBP* VE/VCO 2 provides a relatively better predictive value for all-cause death of CHF patients.