Objective To investigate the breast cancer neoadjuvant chemotherapy for clinical applica-tion.Methods Given suitable breast cancer patients anthracycline-based combination chemotherapy, 2 ～ 4 cy-cle after the surgery were observed to assess their clinical efficacy and conform to conditions and are compatible with the same period did not receive neoadjuvant chemotherapy for a comparative analysis of cases.Results Ac-ceptance of neoadjuvant chemotherapy group of cases the total effective rate was 89.47%, 1-year and 3-year over-all survival rate significantly improved, 17 cases due to neoadjuvant chemotherapy benefit received axillary lymph node dissection to narrow the scope of the modified radical mastectomy for breast cancer, five cases of implemen-tation of the breast retain on the radical operation of breast cancer.Conclusions Neoadjuvant chemotherapy can effectively reduce the clinical stage of breast cancer, some patients benefit from breast-implementation, such as radical mastectomy or surgery to narrow the scope, postoperative survival and prognosis of breast cancer is there-fore improved.

Objective To study the diagnosis and treament of adrenal cortex and medulla hyperplasia. Methods The clinical data of 3 cases of adrenal cortex and medulla hyperplasia were analysed.All the 3 patients had hypertention and elevation of VMA and 1 had hypokalemia.Abnormal CT manifestations have been observed in all. Results Left adrenectomy or subtotal adrenectomy is done for the patients.1 patient recurred 6 months later and was cured by total adrenectomy on the right side. Conclusions Adrenal cortex hyperplasia could be secondary to medullary hyperplasia.When medulla hyperplasia is properly managed,cortex hyperplasia may automatically subside.

Objective To evaluate the effect of finasteride on perioperative bleeding following transurethral plasmakinetic resection of prostate (PKRP).Methods A total of 118 patients with benign prostatic hyperplasia (BPH) undergoing PKRP were randomly divided into three groups:40cases received 5mg of finasteride daily for 7 days before surgery as 1 week group,38 cases received 5mg of finasteride daily for 3 months before surgery as 3 months group,the other 40 cases without taking finasteride before surgery as control group.A comparative study of clinical data was made among the three groups.Results The operation was successfully completed in 118 cases.As compared to control group,intraoperative irrigating fluid volume[(31.5 ± 5.6) L vs.(26.4 ± 6.2) L and (24.3±5.2)L],intraoperative blood loss[(173.5± 16.9) ml vs.(163.5± 15.8) ml and (156.4±16.2) ml],loss of 1 gram prostate tissue for resection[(8.6±4.8)ml/g vs.(7.4±5.4) ml/g and (6.6±5.6) ml/g]and operation time(72.5±16.2) min vs.(58.4±17.8) min and (56.7±16.5) min in 1 week and 3 months groups with taking finasteride were decreased (all P＜0.05).And there were differences in the above indexes (all P＜ 0.05) between 1 week group and 3 months groups.Conclusions The use of finasteride before PKRP is safe and reliable to reduce perioperative bleeding in BPH patients.Moreover,taking 5mg of finasteride for three months is of better effectiveness than taking 5mg of finasteride for 1 week.

Objective To evaluate one-stage arthroscopic reconstruction of anterior crueiate ligament (ACL)and posterior cruciate ligament(PCL)using Achilles tendon-bone allografts. Methods From July 2000 to February 2005.we treated 15 patients(11 males and 4 females)whose ACL and PCL were ruptured at one knee but the eontralateral knee was intact.Their associated meniscus injuries were treated arthroscopically according to established procedures prior to ligament reconstruction.Thirty Achilles tendon-bone allografts were used to reconstruct torn ACL and PCL in 15 knees at one stage.Reconstruction of both ligaments was performed at subacute or chronic phase(>3 to 8 weeks)in 12 casses,and at acute phase in 3 cases(<3 weeks).All knees were graded pre-and postoperatively using the International Knee Documentation Committee(IKDC)and Lysholm scoring systems.At follow-up,functions were evaluated for all patients and compared with those of the contralateral healthy knee. Results All patients were followed up for a minimum of 3 years(mean,38 months).Preoperatively,the IKDC ratings showed all the injured knees were severely abnormal.At final postoperative f0Uow-up,9 knees received a normal rating,5 a nearly normal one and 1 an abnormal one.The differences in Lysholm score were statistically significant (t=15.660,P<0.05)between pre-and postoperative analyses.The most noticeable postoperative complication was a short localized fever coupled with arthroedema in 1 case. Conclusions Achilles tendon-bone allograft offers an alternative for simultaneous arthroseopic reconstruction of ACL and PCL.However,problems inherent in allograft tissues entail further investigation to ensure future application.

Objective To study human lung adenocarcinoma cell line A-549 treated with antagonist and agonist of potassium channel how to affect metastasis of A-549 and its mechanism. Methods Invasion and migration capability of A-549 in vitro was evaluated by using transwell chamber model. Alteration of cytoskeleton was observed through immunofluorescence. Western blotting were used to detect the protein expression of Ezrin and HuR in A-549 cell lines while Glibenclamde and Pinacidil were applied to them. Results In the presence of the antagonist Glibenclamide, migration of A-549 was inhibited by (57.18±5.46)% and invasion was inhibited by (54.92±3.72)% in the transwell assay, meanwhile A-549 manifested disorder of microtubule and more orderly microfilament. And agonist of the potassium channel had an contrary effect on A-549. Ezrin and HuR protein were successfully down-regulated in A-549 treated with Glibenclamide and upregulated in A-549 treated with pinacidil. Conclusion Functional alterations of the potassium channel affects capability of migration and invision of A-549, which is associated with different expression of ezrin and HuR protein that modify cytoskeleton.

Objective The present study aims to determine the correlation between liver function dam-age and hepatitis B virus ( HBV ) reactivation caused by chemotherapy , and the preventive effect of entecavir on HBV reactivation in lung cancer with HBV carriers .Methods A total of 160 lung cancer patients with HBV car-riers in the affiliated tumor hospital of Harbin Medical University from January 2011 to December 2012 was inves-tigated and the clinical data were studied retrospectively .The patients were divided into prophylactic group ( n=80)and control group(n=80).In prophylactic group,0.5 mg of daily oral entecavir was administered before the chemotherapy until 6 months after the completion of chemotherapy .Control group received no entecavir .The inci-dence of HBV reactivation ,functional damage of liver ,toxicities and disruption of chemotherapy were measured . Results The comparison between the control group (25%) and prevent group (5%) showed a statistically signifi-cant difference in the incidence of HBV reactivation (P<0.01).Moreover,HBV-DNA level(HBV-DNA≥104 copies/mL)was risk factors of HBV reactivation (P<0.05).Histology and stage of lung cancer,the chemother-aphy scheme containing platinum , positive HBeAg were not significantly correlated with HBV reactivation ( P>0.05).There were significant differences in grade III and IV hepatic toxicity (P<0.05)between control group (30%)and prevent group(5%),but was not in grade I and II hepatic toxicity (P>0.05).Disruption of chemo-therapy showed significant difference between control group (20%)and prevent group(5%)(P<0.05).The ma-jor grade 1 ~2 toxicities were myelosuppression,nausea,vomiting,skin rash,diarrhoea,neurotoxicity,fatigue, headache,insomnia,etc.All adverse reactions were cured after treatment .Conclusion The prophylactic adminis-tration of oral entecavir could reduce the risk of HBV reactivation in lung cancer with HBV carriers .

Objective To explore the expression of Tiaml in clear cell renal cell carcinoma and analyze its correlations to pathology of disease and prognosis.Methods The expressions of Tiam1 protein in 107 specimens of human clear cell renal cell carcinoma and 20 specimens of normal renal tissues were detected by immunohistochemical staining and its clinical significance was then analyzed.Results The expression of Tiam1 protein was higher in renal cancers than in the adjacent normal tissues ( P ＜ 0.01 ).Tiam1 protein expression rates were 47.6％ and 72.7％ in Ⅰ - Ⅱ and Ⅲ - Ⅳ tumors,while 49.3％ and 76.5％ in T1 - T2 and T3 - T4 tumors,respectively ( P ＜ 0.01 ).Expression of Tiam1 protein was higher in lymph node positive renal carcinoma tissues than in lymph node negative renal carcinoma tissues ( 71.7％ versus 47.5％,P ＜ 0.05 ).The expression of Tiam1 in carcinoma tissues showed a positive relationship with tumor vascular invasion (81.3％ versus 48.0％,P ＜ 0.01 ).In patients followed-up 5 - 8 years,Kaplan-meier analysis and the log-rank test showed that the 5-year survival was significantly different between the group of lower and higher Tiaml expression groups ( 84.4％ versus 46.8％,P ＜ 0.05 ).Conclusions The expression of Tiaml protein was higher in human primary renal carcinoma than in normal renal tissues.The positive rate of Tiam1 protein expression was related to classification,TNM stage,lymph node metastasis and vascular invasion.The detection of the expression of Tiaml protein may be helpful in the diagnosis and prognosis of renal carcinoma.

Objective To investigate the potential application of human transforming growth factor-beta-1 (hTGF-β1) gene mediated by type 2 recombinant adeno-associated virus (rAAV2) vector inducing chondrogenic differentiation of canine mesenchymal stem cells (MSCs) in vitro. Methods Canine MSCs from bone marrow were isolated and cultured in vitro by density gradient centrifngation and adherence screening methods. The morphology of MSCs was observed by inverted phase contrast microscope and Giemsa stain. Flow eytometry was used to detect surface antigens of MSCs, The third generation of MSCs were transfected by rAAV2-hTGF-β1 with or without MOI of 1 ×105 v.g./cell or 5×105 v.g./cell. The expression of hTGF-β1 was detected by Western blot after 10 days, and TGF-β1 synthesis was determined by ELISA at 3, 6 and 9 day, respectively. After 2 weeks of culturing, mRNA expressions of type Ⅱ collagen and aggrecan were determined by RT-PCR and the collagen Ⅱ protein was detected by immunocytochemistry. Results The MSCs appeared to be morphologically spindle-shaped and showed active capability of proliferation both in primary and passage generations. Flow cytometry analysis indicated that MSCs were universally positive for CD29, CD44 and CD105, but negative for CD34 and CD45. TGF-β1 expression can be observed by Western blot after 10 days in two transfection groups, MOI of 5 × 105 group and MOI of 1× 105 group. With the extension of time, the contents of hTGF-β1 increased in the two groups detected by ELISA, while there was a significant difference between them two (P < 0.01). After 2 weeks of transfection of MSCs by rAAV2-hTGF-β1, the expression of collagen Ⅱ and Aggreacan mRNAs were positive. It also showed positive of collagen Ⅱ detected by immunocytochemistry. Conclusion Canine MSCs show chondrogenesis differentiation after induction by Type 2 rAAV mediated transfer of TGF-β1 gene. The process is a potential application for cartilage tissue engineering.

Objective To investigate the diagnostic value of MRI in chronic completely tear of the anterior cruciate lig-ament(ACL). Methods From January 2013 to September 2015 in our hospital,the clinic and MRI data of 20 cases with chronic completely tear of the ACL proved by arthroscopy. Results The diagnostic accuracy rate of MRI was 75%(15/20), and 25%(5/20) was misdiagnosed as partial tear.The MRI signs were: ACL ligament direction (n=20, 100%), abnormal ACL increased signal (14 cases, 70%), tibia forward (n=19, 95%), abnormal deeper lateral femoral notch or bone contusion or cartilage erosion(n=20, 100%), smaller bending angle index of posterior cruciate ligament(n=20, 100%), with the medial meniscus injury(n=18, 90%). MRI also clearly showed other lesions in the knee joint. Conclu-sion MRI has high diagnostic accuracy in chronic completely tear of the ACL, so which is worthy of application.

Objective To investigate the MRI findings of displaced bucket-handle tear in medial meniscus. Methods From April 2011 to August 2015 in our hospital, the clinic and MRI data of 12 patients with displaced bucket-handle tear in medial meniscus confirmed by arthroscopy were retrospectively analyzed. Results 12 cases were unilateral. Fragment shift sign, double posterior cruciate ligament sign, flipped meniscus sign and double meniscus forefoot sign, abnormal circumferential meniscus sign, bow tie broken sign and disproportional posterior horn sign could be seen in all cases. Conclusion Displaced bucket-handle tear in medial meniscus has some characteristic manifestations on MRI, MRI can be used as a preferred imaging methods for displaced bucket-handle tear in medial meniscus.