BACKGROUND:The fiber-reinforced composite resin adhesion fixed bridge produced by everStick fibre reinforced composites is a kind of minimaly invasive fixed prosthesis technology in recent years, which is wel thought of patients and clinicians because of its smal tooth preparation, high success rate of bonding, good esthetic effects, strong mechanical properties and easy to intraoral repair. OBJECTIVE:To investigate the advantage of everStick fibre reinforced composites on too smal anterior edentulous space restoration. METHODS:A total of 23 patients with maxilary lateral incisor missing and with smal edentulous space were colected and renovated with everStick fibre reinforced composites produced by StickTech in Finland. RESULTS AND CONCLUSION:everStick fibre reinforced composites for repair of too smal anterior edentulous space with adhesion fixed bridge could not only avoid the overclosed occlusion on adjacent teeth like fixed denture, but also avoid the usage of clasp and uncomfortableness such as removable denture. During the folow-up of 6-30 months, 23 patients had no signs of loosening, debonding, fracture, excessive wear, secondary caries, margin dying of restoration and exhibited good margins and composite luting-cement interface, unchanged color, good esthetic effects. The patients were very satisfied with the restoration. The results indicate that everStick fibre reinforced composites for restoration of too smal anterior edentulous space can achieve good abrasion resistance and high mechanical strength, unchanged color, have little damage to abutment and have good esthetic effects.

BACKGROUND:As a kind of fiber composite resin adopt the technology of fibre reinforcement composites, the everStick high-strength fiber composite resin has been applied in the field of dentistry prosthodontic treatment restoration.
OBJECTIVE:To summarize the research condition about the material performance and clinical application of the everStick high-strength fiber composite resin
METHODS:We searched the Medline, CNKI, and WanFang database in computer (from January 2000 to August 2013) using the keywords of“everStick high-strength fiber composite resin, material performance, clinical application”in English and Chinese.
RESULTS AND CONCLUSION:The everStick high-strength fiber composite resin has the unique structure of IPN, and has the characteristics of simple operation, minimal y invasive, higher strength, strong cohesive force and reversibility. Because of the minimal y invasive, the everStick high-strength fiber composite resin can retain the healthy tissue of tooth with whole hog and provide the possibility for other therapeutic method because of the reversibility. At the present time, the everStick high-strength fiber composite resin has already been extended in the fields of post core, periodontal splint, orthodontic retainer, ful crown, adhesive fixed bridge, fixed bridge, inlay fixed partial dentures, dental implant restoration and the base in removable partial denture. But whether the material can be made into resin veneer and be used in color front teeth, for instance, the dental fluorosis, tooth discoloration after root canal therapy, tetracycline pigmentation teeth and hypoplasia of enamel, there is lack of comprehensive studies.

Plasmodium yoelii yoelii-Anopheles stephensi system was chosen as the experimental model in studying the effects of pyrimethamine on oocyst formation of the plasmodium species. The drug was given by allowing mosquitoes to feed on infected and pyrimethamine treated mice or by feeding them directly with pyrimcthamine-sugar water. The infective rate and the number of oocysts formed after drug administration were reduced, the oocysts formed being smaller and their daily growth rate slower than that of the controls. Electron microscopic and Feulgen staining studies showed that the cytoplasm of the affected oocysts contained many vacuoles, pigment aggregations and black aggre-gates (Fig. 4). No nucleus appeared in the affected oocysts, which were presumably deteriorated and became "black spores". The amount of DNA in drug-affected oocysts was scanty. No sporozoites were found in the salivary glands of these mosquitoes. It was suggested that pyrimethamine interfered with DNA synthesis of oocysts.

Streptolysin S (SLS),one of the important virulence factors of Streptococcus,exist in several kinds of human and animal pathogenic bacterial,including Streptococcus pyogenes,Strepstococcus iniae and Streptococcus anginosus.SLS is a peptide toxin encoded by nine consecutive genes (sagA-sagⅠ).The functions of SLS include contributing pathogenic bacterium to pass through epithelial barrier,causing tissue damage,resisting to phagocytic clearance of host immune cells and interacting with other virulence factors.In addition,SLS as a signaling molecule of cell quorum sensing is involved in regulating the expression with other virulence factors.This paper summarized the structures and the biological functions of SLS in Streptococcus infection.

Objective To observe the effects of matrine, oxymatrine and sophordine on activity and TNF-? secretion of macrophage RAW264.7. Methods Macrophage were cultivated in 6 holes board and cultivated 1 day with different concentration matrine, oxymatrine and sophordine. Macrophage activity was measured with MTT and content of TNF-? in collected culture medium was measured with ELISA. Results Macrophage activity and the function of secreting TNF-? was restrained by matrine, oxymatrine and sophordine. The higher concentration of matrine, oxymatrine and sophordine was, the more intense the inhibiting effect was. The effect of matrine was more intense to the function of secreting TNF-? of macrophage. Conclusion Matrine, oxymatrine and sophordine can restrain macrophage activity and the function of secreting TNF-?, the effect of matrine is the most intense.

Objective To develop an apoptosis model of nucleus pulposus cells in cell culture.Methods To mimic the nutrient-deficient microenvironment of degenerative intervertebral disc,nucleus pulposus cells derived from infant SD rat disc were cultured under serum limiting conditions.Nucleus pulposus cells were cultured in culture medium contai-ning 1%, 3%, 5%, 8%and 10%fetal bovine serum( FBS) respectively to select the optimum FBA concentration.Apoptosis was assessed by flow cytometry, Western blot,cell counting kit, and immunofluorescence technique.Results The flow cy-tometry revealed that apoptosis rate of the nucleus pulposus cells increased with decreasing concentration of FBS, and 3%FBS used in the experimental group was the most effective concentration to induce apoptosis(P<0.05).Western blot dem-onstrated significantly higher expression of Bax and caspase-3 enzyme in the 3%FBS group than in the 10%FBS group, while bcl-2 activity decreased.The results of CCK-8 test indicated that the nucleus pulposus cells got slower proliferation in the medium containing 3%FBS.Immunofluoresence analysis showed that FAS expression was significantly higher in the 3%FBS group than in the 10%FBS group.Conclusions 3%FBS condition may induce apoptosis in the nucleus pulposus cells and compromise the cell function to induce intervertebral disc degeneration.The caspase family should be involved in the process.

With light and electron microscope, we studied the morphology of the thymus of the neonatal mouse. The results showed: 1.the lobules of the thymus had not well developed and there was no distinct demarcation between the cortex and medulla; 2.a cyst composed of epithelial cells with microvilli or cilia might be frequently seen in the medulla; 3.occasionally small lymphocytes with some glycogen particles in their cytoplasm were observed; 4.only a few small-sized thymic corpusles existed in the medulla, The article also described the ultrastructure of the lymphocytes, epithelial reticular cells, macrophage, interdigitating cell and blood-thymus barrier in the thymus of the neonatal mouse.

Objective To explore the expressions of CK20, S100A7 and substance P (SP) in different stages of psoriatic lesions and their relationship. Methods A total of 19 patients, who had received irregular treatment for psoriasis and had both progressive and healed psoriatic lesions, were enrolled in this study. Skin tissue specimens were obtained from perilesional normal skin, progressive lesions and healed lesions of these patients and subjected to immunohistochemical analysis of expressions of CK20, S100A7 and SP. Results The relative expression level (absorbance value obtained from immunohistochemical analysis) was 7683.80 ± 6134.55,18305.04 ± 13171.30, 7257.53 ± 4417.75 for CK20, 8789.05 ± 6240.91, 18058.01 ± 16537.18, 9295.65 ±9310.02 for S100A7, 3242.51 ± 3775.41, 9364.98 ± 7596.64, 2910.85 ± 3349.46 for SP, respectively, in normal skin, progressive lesions and healed psoriatic lesions. A significant increase was observed in the expressions of CK20, S100A7 and SP in progressive lesions compared with normal skin and healed lesions, whereas no statistical difference was noted in those between normal skin and healed lesions (P ＞ 0.05 ). The expression of CK20 was positively correlated with that of S100A7 and SP (r = 0.779, 0.876, both P ＜ 0.05 ). Conclusion The pathogenesis of psoriasis seems to be associated with the changes in the number of Merkel cells.

Objective To evaluate the potential feasibility of γ-H2AX foci as a biodosimetry after exposure to ionizing radiation by comparing DNA double-strand break repair kinetics in rat blood lymphocytes with that in human lymphocytes.Methods Peripheral blood lymphocytes separated from Sprague-Dawley(SD) male rats and healthy adults were exposed to γ-rays,and some rats were also subjected to total body irradiation.The inductions of DNA repair-related foci of γ-H2AX,pATM (S1981) and pDNA-PKcs (T2609) were detected with immunofluorescence staining technique at different time points post-irradiation,and the status of their co-localization was analyzed.Results The induction kinetics of γ-H2AX foci in rat lymphocytes was similar to that observed in human lymphocytes.The frequencies of γ-H2AX foci peaked at 30 min after γ-ray irradiation (trst =62.64,th =28.52,P ＜ 0.05),then decreased rapidly after 6 h post-irradiation (trat =45.96,th =14.80,P ＜0.05),and the residual foci number remained only about 3％-8％ of its maximal value at 24 h post-irradiation.At 30 min after γ-ray irradiation,the frequencies of pATM (S1981) and pDNA-PKcs (T2609) foci in rat and human lymphocytes significantly higher than those of nonirradiated control (trat =21.05,25.80,th =11.07,29.52,P ＜ 0.05),and the frequencies of co-localization of pATM (S1981) or pDNA-PKcs (T2609) foci with γ-H2AX foci also markedly increased by 26％-32％ in irradiated lymphocytes of rat and human (trat =5.34,9.14,thuman =18.32,51.28,P ＜0.05).Moreover,γ-H2AX foci incidence in rat lymphocytes in vitro was consistent with that induced by total body irradiation of rat.The number of γ-H2AX foci in irradiated rat lymphocytes increased with irradiation dose in a linear dose-dependent manner,its slope was similar to that of irradiated human lymphocytes reported by other laboratory.Conclusions Rat is a useful animal model to evaluate radiation biodosimetry with γ-H2AX foci in lymphocytes.The co-activation of ATM and DNA-PK plays an important role in DSB repair in the irradiated lymphocytes of rat and human.

Objective To investigate the apoptosis of bone marrow mesenchymal stem cells(BMSCs)from systemic lupus erythematosus(SLE)patients and the expression of apoptotic molecules.Methods BMSCs were isolated from bone marrow of SLE patients and normal controls by density eentrifugation and adhesive culture in vitro.The apoptosis of BMSCs were evaluated by TUNEL assay.The expressions of Fas.Bcl-2 and the activity of Caspase 8 were detected by flow cytometry.Real-time PCR technique was used to determine the gene expressions of Fas,Bcl-2,Bax,Bcl-w,Caspase 8 and Apaf-1.Meanwhile,cytochrome C was detected by immunocytochemistry.Statistical analysis was conducted with t-test and Mann-Whitney rank test.Results The percentage of apoptotic BMSCs increased in SLE patients compared with healthy donors[(64±10)%vs [14±9)%,U=0,P<0.05 ].The expression of Bcl-2 in BMSCs of SLE patients was lower than the normal controls at protein leve[(11±9)%vs(56±18)%,U=0,P<0.05 ],and mRNA level(0.2±0.2vs 2.4±0.7,U=24,P<0.05).More cytochrome C positive pellets in the cytosolic fraction could be detected in BMSCs from SLE patients compared with healthy controls [(56±21)%vs (16±16)%,U=1,P<0.05].The activity of Caspase 8 was enhanced[(49±14)%vs(16±12)%,U=0.P<0.05 ],although with no significant difierence at mRNA Ievel.Both groups expressed Fas but with no significant difference (U=19,P>0.05).Conclusion BMSCs from SLE patients undergo more apoptosis,the mechanisms may be associated with the down regulation of Bcl-2,up-regulation of Cytoehrome C in cytoplasm and the activation of Caspase 8,which directs the intrinsic and extrinsic apoptosis pathways.