1.Determination of 4′-O-?-D-glycosyl-5-O-methylvisamminol in Jingfuzhiyang Granules by RP HPLC
Chinese Traditional Patent Medicine 1992;0(11):-
AIM: To establish a RP HPLC method for the determination of 4′ O ? D glycosyl 5 O methylvisamminol in Jingfuzhiyang Granules (Herba Schizonepetae, Fructus kochiae, Radix Saposhnikoviae, Fructus Crataegi, etc.). METHODS: The sample was separated on Turnner Kromasil C 18 column (4.6mm?200mm,5?m) with mobile phase of methanol 0.05mol?L -1 potassium dihydrogen phosphate acetic acid isopropyl alcohol (85∶170∶4∶4,v/v). The detection wavelength was set at 254nm and the flow rate was 1.0mL?min -1 . The column temperature was 30℃. RESULTS: The calibration curve was linear in the range of 0.0606~0.606?g (r=0.9994). The average recovery was 92.2%. CONCLUSION: This method proves to be relatively simple and accurate, and available for the quality control of Jingfuzhiyang Granules
2.Determination of geniposide in Xiaobanlifu Unguentum by HPLC
Chinese Traditional Patent Medicine 1992;0(05):-
Objective: To establish the determination method of geniposide in Xiaobanlifu Unguentum. Methods: HPLC was performed to determine the content of geniposide on Inertsil ODS-3 column, using methanol-potassium dihydrogen phosphate-acetic acid-isopropyl alcohol (67∶173∶4∶4,v/v) as a mobile phase and detection wavelength at 238nm. Results: The linearity of this method was good, and the averge recovery was 97.3%, RSD=1.94%. Conclusion: This method was relatively simple and accurate, and available for the quality control in Xiaobanlifu Unguentum.