0.05). However, the scores of belief and attitude of experimental group were higher than those of control group (t=3.03～19.89, P0.05), while the score in control group displayed decreasing trend (t=5.75, P

This paper reports one case of elderly patients with bronchial foreign body, and analyzes the causes of misdiagnosis. And the best choice of removing larger foreign body is operating under rigid bronchoscopy.
Aged, 80 and over ; Bronchi ; Foreign Bodies ; Humans ; Male

Aim To detect the effect of Bio on impro-ving insulin resistance and explore its molecular mech-anism. Methods The HepG2 liver cells were derivat-ed by high concentration insulin to establish the insulin resistance cell model, and the cells were intervened by Bio. The glucose consumption was measured by glu-cose oxidase and peroxidase ( GOD-POD) assay. The expression of PPARγmRNA was detected by RT-PCR. The expression of PPARγ protein was detected by Western blot method. Results The glucose consump-tion was significantly decreased in the insulin resist-ance cells after incubated with 1 . 72 × 10 -5 mol · L-1 insulin ( P<0. 05 ) . 10 -5 ,10 -6 ,10 -7 mol · L-1 Bio increased the glucose consumption 135%,62%,39%separately in the insulin resistance cells. RT-PCR a-nalysis of PPARγ showed that Bio raised the PPARγmRNA. Western blot analysis displayed that the pro-tein of PPARγ with Bio was increased. Conclusion Bio can improve the insulin resistance of the HepG2 cells, and the molecular mechanism may be relevant with raising PPARγ expression.

Objective To investigate the clinical significance of peripheral blood T-helper (Th22) cells in assessing the severity and prognosis of patients with sepsis.Methods A total of 220 patients with sepsis in our hospital from January 2013 to January 2017 were enrolled in the study.According to the severity of sepsis,these patients were divided into low risk group (84 cases),middle risk group (74 cases) and high risk group (62 cases).According to the clinical outcome of sepsis,these patients were divided into survival group (195 cases) and death group (25 cases).The levels of Th22 cells,IL-22 and CRP in the peripheral blood were measured,and the acute physiology and chronic health status (APACHE Ⅱ) score were recorded,the predictive value of peripheral blood Th22 cells in deathof sepsis was assessed by the receiver operating characteristic curve (ROC).Results The levels of Th22 cells,IL-22 and APACHE Ⅱ in the low-risk group,the intermediate-risk group and the high-risk group were statistically significant (P ＜ 0.05).The high-risk group was highest,followed by the intermediate-risk group,the low-risk group was lowest among them.The levels of Th22,IL-22 and APACHE Ⅱ scores of the death group were significantly higher than those in the survival group (P ＜ 0.05).The correlation analysis showed that the Th22 celss in peripheral blood were positively correlated with IL-22 (r =0.70,P ＜ 0.01) and APACHE Ⅱ scores (r =0.75,P ＜ 0.01).When Th22 cells in peripheral blood ＞ 3.3％ for the assessing the poor prognosis of sepsis boundaries,the sensitivity and specificity were 84.4％ and 86.1％.Conclusion The Th22 cells in peripheral blood and IL-22 are closely related to the severity and prognosis of sepsis,serving as an assessing index and have important clinical value.

Objective To evaluate the efficiency of nursing human resources in the nursing unit of a severe medical department in a hospital by data envelopment Analysis (DEA), to explore the reasons of inefficiency and to provide basis for improving nursing efficiency. Methods Three nursing input indicators from January 2017 to December in a certain ICU (the actual number of beds in the month department, the number of nurses in the department, the actual number of working hours in the department), and 2 indicators of output indicators (total number of hospitalized patients in this month, total number of patients admitted this month) related data were selected. Using DEA to analyze and study. Results From January 2017 to December, the overall efficiency was 0.959, and there were 3 units (accounting for 25％) in overall efficiency =1, pure technical efficiency was 0.984, 6 units of pure technical efficiency =1 (accounting for 50％), and scale efficiency was 0.974, there are 3 units of scale efficiency =1 (accounting for 25％). Conclusion There is significant difference in the level of serious nursing efficiency in our hospital, and the efficiency can be maximized through rational allocation of nursing human resources.

Objective:To investigate the factors related to recurrent laryngeal nerve invasion in papillary thyroid carcinoma (PTC) with posterior capsular involvment.Methods:The data of 186 PTC patients admitted and operated from Jun 2017 to Jun 2022 were retrospectively analyzed. The invasion of recurrent laryngeal nerve was evaluated on its relation to gender, age, tumor size, Hashimoto's thyroiditis, lymph node metastasis in central region, BRAFV600E gene mutation especially PTC posterior capsular involvement.Results:The recurrent laryngeal nerve was invaded in 30 out of 186 patients. Univariate analysis showed that recurrent laryngeal nerve invasion was related to tumor size, Hashimoto's thyroiditis and cervical lymph node metastasis( χ2=6.964,4.814,6.078, P<0.05). Multivariate regression analysis showed that tumor size and lymph node metastasis in cervical region were independent risk factors for recurrent laryngeal nerve invasion(β=1.020,1.622, P<0.05). Hashimoto's thyroiditis was a protective factor for recurrent laryngeal nerve invasion (β=-1.881, P<0.05). Conclusions:When papillary thyroid carcinoma invaded the capsule, the risk of recurrent laryngeal nerve invasion was higher with larger tumor size and cervical lymph node metastasis, while Hashimoto's thyroiditis was a protective factor for the risk of recurrent nerve invasion.

Objective:To investigate the factors affecting the change of serum calcium and phosphorus after total parathyroidectomy for secondary hyperparathyroidism(SHPT).Method:Sixty patients with secondary hyperparathyroidism undergoing total parathyroidectomy were selected.Gender, age, hemodialysis time, bone pain time, intravenous calcium supplementation time, blood parathyroid hormone(PTH), parathyroid mass, blood calcium, phosphorus and alkaline phosphatase at admission were tested to compare the correlation between postoperative calcium and phosphorus changes.Result:Blood calcium was: (2.50±0.02), (2.27±0.03), (1.97±0.03), (1.95±0.03), (1.95±0.03), (2.03±0.02) mmol/L at admission, 6 hours after surgery, 1, 2, 3 days after surgery, and when discharged. Blood phosphorus was: (2.32±0.07), (1.95±0.05), (1.65±0.06), (1.44±0.05), (1.35±0.36), (1.14±0.04) mmol/L and when discharged.There are differences between pairwise comparisons ( F=100.979, P<0.05; F=118.910, P<0.05). The blood PTH was (1 791±760) pg/ml, and the postoperative intravenous calcium supplementation time was (7.13±2.34) days. Blood PTH was positively correlated with bone pain time, blood phosphorus and parathyroid mass at admission ( t=-2.199, P<0.05; t=4.718, P<0.05; t=2.507, P<0.05). The time of intravenous calcium supplementation was positively correlated with ALP at admission ( t=3.098, P<0.05). Preoperative serum PTH > 1 430 pg/ml or ALP > 191 mmol/L were prone to hypocalcemia on the first day after surgery. Conclusions:The longer bone pain time, the higher blood phosphorus and the more obvious parathyroid hyperplasia were, the higher PTH was. The higher the ALP, the longer the time of intravenous calcium supplementation. Preoperative serum PTH and ALP values can be used as predictors of hypocalcemia on the first day after surgery.

Eighty samples of Epimedium from 29 species and were determined in this study. The content of magnoflorine in leaves range between 0. 003% and 2. 603%. The results showed that the content of magnoflorine was quite stable within species except E. wushanense, E. acuminatum, E. hunanense. Genetic factors might be the main influencing ones. The contents of different parts and different collecting time of the medicinal materials were variable.
Aporphines ; chemistry ; metabolism ; Breeding ; Drugs, Chinese Herbal ; chemistry ; metabolism ; Environment ; Epimedium ; chemistry ; classification ; metabolism ; Plant Leaves ; chemistry ; metabolism ; Species Specificity ; Tissue Distribution

Female ; Humans ; Papillomaviridae ; Uterine Cervical Neoplasms ; pathology ; virology ; Vaginal Smears

Objective:To explore the clinical features and genetic etiology of a child with Char syndrome.Methods:A child who was presented at the Department of Child Health, Henan Children′s Hospital in February 2022 was selected as the study subject. Clinical data of the child was collected, and peripheral blood samples of the child and her parents were collected for the extraction of genomic DNA. Whole exome sequencing was carried out, and candidate variants were verified by Sanger sequencing and bioinformatic analysis.Results:The child had mainly manifested facial dysmorphism, patent ductus arteriosus, growth retardation, curving of fifth fingers and middle toes. Whole exome sequencing revealed that she has harbored a heterozygous c. 944A>C (p.Glu315Ala) variant of the TFAP2B gene, which was verified to be de novo by Sanger sequencing. Based on the guidelines from the American College of Medical Genetics and Genomics (ACMG), the variant was rated to be likely pathogenic (PM1+ PM2_Supporting+ PM6+ PP3). Conclusion:The heterozygous c. 944A>C (p.Glu315Ala) variant of the TFAP2B gene probably underlay the Char syndrome in this child. Above finding has expanded the mutational and phenotypic spectra of the TFAP2B gene, which has facilitated early identification and diagnosis of Char syndrome.