1.Research on the Effect of Electro-acupuncture of Neiguan on the CGRP Content in Migraine Rats' Plasma
Journal of Zhejiang Chinese Medical University 2007;0(01):-
[Objective] To study the effect of eletro-acupuncture of Neiguan on the CGRP in rats' plasma and its functional mechanism.[Method] Inject subcutaneously GTN to make rats migraine model.After electro-acupuncturing Neiguan,take radio-immune method to measure the CGRP content in rats' plasma of experimental group.[Result] Electro-acupuncturing Neiguan can reduce rats CGRP content.[Conclusion] To inject subcutaneously GTN can raise rats' plasma CGRP content,electro-acupuncturing Neiguan can reduce risen CGRP,regulate vascular extension,improve nerve inflammation,thus relieve migraine symptoms.
2.Influence of Transdermal Electric Stimulation of Neiguan (PC 6) On TCD in Patients with Acute Migraine
Debao YE ; Jianqiao FANG ; Yinghui SONG ; Shuyan ZHANG ; Zeyun MA ; Weiai LIU
Journal of Acupuncture and Tuina Science 2006;4(1):22-24
Purpose: In order to explore the clinical mechanism of Neiguan (PC 6) in the treatment of acute seizure of migraine and the relationship between Neiguan (PC 6) and cerebral blood flow. Methods: Clinically, 30 cases of the patients with acute seizure of migraine (ascending phase) were selected, in TCD abnormality, and divided randomly into the treatment group and control group, 15 cases in each group. In acute seizure of migraine, Neiguan (PC 6)was punctured with electric stimulation on the healthy side of the patients in the treatment group and Xuehai (SP 10) was punctured with electric stimulation on the healthy side of the patients in the control group. TCD was determined in the patients 30 min before and after the treatment. Results and conclusion: After transdermal electric stimulation on Neiguan (PC 6),the velocity of intracranial blood flow was obviously slowed down, indicating that transdermal electric stimulation on Neiguan (PC 6) can effectively relieve the spasm of intracranial blood vessels and also shows its relative specificity.
3.Expression of Plk1, Cyclin B1 and p21WAF1 in cervical carcinoma and their clinical signifi-cance
Yunna QIN ; Deming HE ; Debao ZHUANG ; Lu YE ; Yinghui DENG ; Hailian LUO ; Xiaohong YU
Chinese Journal of Clinical and Experimental Pathology 2015;(4):385-389
Purpose To investigate the expression of polo-like kinase 1 (Plk1) and Cyclin B1, p21WAF1in cervical carcinoma, and to determine the relationship between the expression of the three proteins and tumor clinicopathological features. Methods The expres-sion of Plk1, Cyclin B1 and p21WAF1 was detected in 102 cases of cervical carcinoma, 20 cases of (cervical intraepithelial neoplasia, CIN) , and 20 cases of nomal cervical tissues by the technique of tissue chip and immunohistochemical staining of EliVision. Statistical analyses of the data were performed with SPSS 19. 0 software. Results The positive rates of Plk1 in cervical carcinoma and CIN were 70. 5%, 55. 0%, respectively, which were significantly higher than normal cervical tissues (10%) (P<0. 01);The positive rates of Cyclin B1 in cervical carcinoma and CIN were 52. 9% and 30. 0%, respectively, which were significantly higher than normal cervical tissues (10%)(P <0.01); The positive rates of p21WAF1 in cervical carcinoma and CIN were 23.5% and 10.0%, respectively, which were significantly higher than normal cervical tissues ( 0 ) ( P<0. 01 ) . There were no significant differences between cervical carcinoma and CIN in the positive rates of Plk1, Cyclin B1 and p21WAF1. The expression of Plk1 was associated with the depth of carci-noma invasion (P<0. 05), that of Cyclin B1 was associated with lymph node metastases and the depth of carcinoma invasion (P<0. 05)and that of p21WAF1 in cervical carcinoma was associated with histological grade (P<0. 05). In cervical carcinoma, the expres-sion of Plk1 was positively correlated with Cyclin B1 (rs =0. 297, P=0. 002) and negatively correlated with p21WAF1(rs = -0. 403, P<0. 001). Conclusion The expression of Plk1, Cyclin B1 and p21WAF1 is involved in the occurrence and development of cervical carcinoma, and the former two are also related with prognosis of cervical carcinoma. The combination of the three would provide a new target for clinical treatment.