OBJECTIVE:To study the quality standard of Changsheng oral solution.METHODS:Radix Astragali,Radix Platycodi,Spica Prunellae and Radix et Rhizoma Glycyrrhizae in the formula were identified qualitatively by TLC.The content of Liquiritin in Changsheng oral solution was determined by HPLC.The chromatographic separation was performed on Nova-Pak C18 column(150 mm?4.6 mm,4 ?m)and the mobile phase consisted of acetonitrile-2.5% acetic acid(13∶87)at a flow rate of 1.0 mL?min-1.The detective wavelength was set at 276 nm.RESULTS:Clear TLC spots were noted.The linear range of Liquiritin was 0.025 04～0.626 00 ?g(r=0.999 9).The average recovery rate of Liquiritin was 98.66%(RSD=0.52%,n=6).CONCLUSION:The established standard can be used for the quality control of Changsheng oral solution.

Bacillus cereus 357 and its antagonistic substance can effectively ant agonize Botytis cinerea Pers and Rhizoctonia solani They can inhibit or kill t he hyphae of Rhizoctonia solani and Botytis cinerea Pers In addition, they can inhibit the growth of hyphae?the formation as well as the germination of spore of Botytis cinerea Pers The antagonistic substance is stable against heat ?acid and alkali and enzyme

Objective To investigate serum angiopoietin-2(Ang-2)levels in non-small cell lung cancer(NSCLC)and its clinical significance.Methods Patients with NSCLC(n=98)and healthycontrols (n=30)were recruited into this study.Serum Ang-2 levels were measured using enzyme-linked immunosorbent assays(ELISA).The results were compared between patients with ifferent tumor stages,metastasis status,and postoperative recurrence 1 year after surgery.Results Patients with NSCLC had higher Ang-2 levels[median 3 230.5 ng/L,range(1 231.6～4 612.5)ng/L ]than the control group [median 831.7 ng/L,range(592.4-1 701.1)ng/L,U=15,P<0.01].Serum Ang-2 levels in stage Ⅲpatients[median 2 311.7 ng/Lrange(1 512.9～3 530.7)ng/L]were significantly higher than stage I [median 1 593.6 ng/L,range(1 235.2～2 763.1)ng/L,H=27.66,P<0.01]and stage Ⅱ patients [median 1 581.4 ng/L,range(1 231.6～2 852.3)ng/L,H=27.66,P<0.01],but significantly lower than stage Ⅳ patients[median 3 808.2 ng/L,range(2 235.8～4 612.5)ng/L,H=65.6P<0.01].Serum Ang-2 levels were higher in patients with distant metastasis or postoperative recurrence than those without them.Conclusion Serum Ang-2 level in patients with NSCLC could be a useful marker for metastasis and postoperative recurrence.

Objective To investigate the Effects of thyroxine on the level of serum homocysteine and urinary albumin excretion rate in elderly patients with early diabetic nephropathy and subclinical hypothyroidism.Methods Seventy-five patients with early diabetic nephropathy and subclinical hypothyroidism were randomly divided into levothyroxine treatment group and conventional treatment group.Urinary albumin excretion rate (UAER),serum levels of homocysteine,creatinine,and lipids were measured at both pre-and post-treatment of 48 weeks.Results After treatment,serum total cholesterol,low density lipoprotein-cholesterol,triglyceride,thyrotropin,homocysteine,UAER,and serum creatinine in the levothyroxine treatment group were significantly lower than those in the conventional treatment group [(-0.52 ± 0.12 vs 0.31 ± 0.40) mmol/L,(-0.33 ± 0.22 vs 0.24 ± 0.36) mmol/L,(-0.16±0.18 vs0.19±0.29)mmol/L,(-4.4 ± 1.2 vs 1.2 ±0.8)mIU/L,(-1.4 ±2.0 vs0.9± 1.0)mmol/L,(-13 ± 13 vs 10 ± 7) pg/ml,(-2 ± 2 vs 3 ± 2) μmoL/L,respectively,all P＜0.01].Conclusions Treatment with levothyroxine could significantly improve serum lipid profiles and reduce homocysteine,UAER,and creatinine,and exert a protective effect on the kidney in the elderly patients with early diabetic nephropathy and subclinical hypothyroidism.

Sixty patients with early diabetic nephropathy (DN) and normal blood pressure were randomly divided into telmisartan treatment and control groups.And the blood biochemical parameters,urinary albumin excretion rate (UAER),the serum levels of homocysteine (HCY) and transforming growth factor-β1 (TGF-β1) were measured at both pre- and post-treatment of 24 weeks.After treatment,the levels ofUAER,HCY [(61 ±26) vs.(98±38) mg/L,(11.70 ±2.86) vs.(14.4±2.8) μmol/L (P＜ 0.01 ) ]and homeostasis model of insulin resistance index,TGF-β1 [ ( 3.17 ± 0.66) vs.( 3.60 ± 0.87 ),(66 ± 14) vs.(77 ± 17 )U/L respectively (P ＜ 0.05 )]in the telmisartan group were significantly lower than those in the control group.It indicated that telmisartan could improve the insulin sensitivity,significantly decrease the serum levels of HCY and TGF-β1,reduce UAER and exert kidney protection effects in the patients with early DN.

Objective To observe the level changes of interleukin-15 (IL-15) and its role and clinical sig-nificance in severe type B hepatitis(HB). Methods IL-15 levels of 47 cases of severe HB and 20 cases of healthy subjects were detected by ELISA,meanwhile the alanine aminotransferase (ALT),total bilirubin (TBIL) and pro-thrombase activity (PTA) were measured as well. The correlation between IL-15 and ALT,TBIL and PTA were ana-lyzed. Results IL-15 in severe HB eases were higher than in control group [(18.9±7.5 ) ng/L vs. (5.9±2.0) ng/L,P <0.01] ,which was higher in death group than in survival group[(24.1±7.5) ng/L vs. (15.7±5.4) ng/L, P<0.01]. IL-15 level was decreasing with the improvement of general condition and liver function recovery. In addition, IL-15 in severe HB was positively correlated with TBIL (r=0.570,P<0.01) and negatively correlated with PTA(r=-0.529,P<0.01) but was not significantly correlated with ALT(r=0.099,P>0.05). Conclusion IL-15 may take part in the pathogenesis of severe HB ,which is consistent with disease condition and is closely re-lated to the improvement of disease. The detection of IL-15 may exert a predicting role in the prognosis of severe HB.

ABSTRACT:Objective To study the expressions of C-Met and hepatocyte growth factor (HGF)in tongue squamous cell carcinoma and their clinical significance.Methods We analyzed 46 patients (the treatment group) with tongue squamous carcinoma and 27 patients (the control group)with benign tumor treated in our hospital between June 201 1 and May 2013.The expressions of C-Met and HGF were detected by immunohistochemistry. Results In the experimental group,C-Met negative expression rate was 21.7% (10/46),weak positive expression rate was 26.1% (12/46),and strong positive expression rate was 52.2% (24/46).The above expression rates were 77.8% (21/27),1 5.8% (5/27),and 3.7% (1/27)in the control group.The two groups differed significantly (P <0.001).In the experiment group,the negative expression of HGF protein was 1 7.4% (8/46 ),weak positive expression rate was 58.7% (27/46),and strong positive expression rate was 23.9% (1 1/46).The above expression rates in the control group was 63.0% (1 7/27 ),37.0% (10/27 ),and 0.0% (0/27 ).The two groups differed significantly (P <0.001).The expression of C-Met protein was significantly related to lymph node metastasis (P <0.05).The expression of HGF protein was significantly related to pathological grading and lymph node metastasis (P <0.05).Conclusion The expressions of C-Met and HGF in tongue squamous cell carcinoma are significantly higher.And the occurrence and development of tongue squamous cell carcinoma are closely related to the expressions of C-Met and HGF.

AIM: To study on antisense oligoncleotides as inhibitor of human acute promyelocytic leukemia (HL-60) proliferation and C-myc protein expression. 　METHODS: Oligonucleotides with different lengths (18-21 mer) complementary to the definite regions of C-myc mRNA, modified groups (with S replaced O in internucleotide phosphate linkage) and unmodified ones (with natural internucleotide phosphate linkage) were designed and synthesized. These olignucleotides were tested for their activity on HL-60 cell and also for their toxicity on normal lymphatic cells of human. 　RESULTS: It was found that two of the oligonucleotides complementary to 5′-44-61 and 5′-556-576 the regions of C-myc mRNA exhibited great inhibitory effects (59.5 % and 62.7 %) on growth of HL-60 cells for a definite time. And no toxicity of the two antisense oligonucleotides was found on normal lymphatic cells of human. 　CONCLUSION: The sequence of antisense oligonucleotides complementary to 5′-44-61 of C-myc mRNA was designed newly by us may be turned into inhibitory medicine of HL-60 cells.

OBJECTIVE: To establish a method for measuring the photoelectric performance index (luminance response characteristic and luminance tolerance) of medical electronic endoscope. METHODS: Based on the clinical application and product features of medical electronic endoscope, the umbrella grayscale test chart and the adjustable gray scale test chart are designed and made from two aspects of distribution and density differential. RESULTS: The influence of gray scale arrangement, background illumination intensity and illumination spectrum on photoelectric performance measurement of electronic endoscope is verified by test. CONCLUSIONS It is a reference for the design analysis, evaluation and modification of electronic endoscope product photoelectric part, whether it is suitable for fast detection of umbels and more accurate gray scale test chart.
Electronics, Medical ; Endoscopes ; Light ; Quality Control