1.FGF-21 protects H9c2 cardiomyoblasts against hydrogen peroxide-induced oxidative stress injury.
Miao-Miao HAN ; Wen-Fei WANG ; Ming-Yao LIU ; De-Shan LI ; Bing ZHOU ; Yin-Hang YU ; Gui-Ping REN
Acta Pharmaceutica Sinica 2014;49(4):470-475
Fibroblast growth factor-21 (FGF-21) is an important metabolism regulator, however, whether FGF-21 has effects on cardiovascular remains unclear. In this study, H2O2-induced injury in H9c2 cells was used as a cell model, the anti-apoptosis potential and mechanism of FGF-21 against oxidative injury were evaluated by MTT assay, flow cytometry assay and real-time PCR. The results showed that FGF-21 could increase the cell survival of H2O2-induced injury in H9c2 cells and prevent H9c2 cells from oxidative stress-induced apoptosis. Furthermore, FGF-21 can elevate SOD activity and regulate Bcl-2/Bax expression in H9c2 cells. The results suggest that FGF-21 have protective effect against the H2O2-induced apoptosis in H9c2 cells.
Animals
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Apoptosis
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drug effects
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Cell Proliferation
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drug effects
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Cells, Cultured
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Fibroblast Growth Factors
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pharmacology
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Hydrogen Peroxide
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toxicity
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Malondialdehyde
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metabolism
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Myocytes, Cardiac
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cytology
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drug effects
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metabolism
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Oxidative Stress
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drug effects
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Protective Agents
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pharmacology
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Proto-Oncogene Proteins c-bcl-2
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genetics
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metabolism
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RNA, Messenger
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metabolism
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Rats
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Reactive Oxygen Species
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metabolism
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Superoxide Dismutase
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metabolism
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bcl-2-Associated X Protein
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genetics
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metabolism
2.Clinical observation on acupoint injection of VitB12 for treatment of trigeminal neuralgia.
Chang-shan ZHOU ; De-qing KONG ; Zheng-yong HAN
Chinese Acupuncture & Moxibustion 2007;27(9):668-670
OBJECTIVETo probe a better method for treatment of trigeminal neuralgia. Methods One hundred and four cases of trigeminal neuralgia were randomly divided into a treatment group (n=57) and a control group (n=47). The treatment group were treated witb acupoint injection of 2000 microg of VitB12, with Xiaguan (ST 7) selected as main point, and the control group witb oral administration of Carbamazepine. The therapeutic effect were analyzed after 3 tberapeutic courses.
RESULTSTbe cured and markedly effective rate and the effective rate were 82.5% and 98.2% in the treatment group, and 57.4% and 8O.9% in the control group, with a very significant difference between the two groups (P < 0.01). After treatment, there was a very significant difference in the cumulative score of pain between the two groups (P < 0.01).
CONCLUSIONAcupoint injection of VitB12 has a better therapeutic effect than that of oral administration of Carbamazepine.
Acupuncture Points ; Adult ; Aged ; Female ; Humans ; Injections ; Male ; Middle Aged ; Trigeminal Neuralgia ; therapy ; Vitamin B 12 ; administration & dosage
3.Research situation of effects of sulfur fumigation on quality of traditional Chinese medicine.
Tu-Lin LU ; Zi-Wan NING ; Xin SHAN ; Lin LI ; Chun-Qin MAO ; De JI ; Fang-Zhou YIN
China Journal of Chinese Materia Medica 2014;39(15):2796-2800
Sulfur fumigation (SF) is a universal phenomenon in primary processing of Traditional Chinese Medicine (TCM) in modern times. In the process, fumigation, sulfur or both of them act on the TCMs. Some active components of TCMs change quantitatively or qualitatively during the processing. At the same time, the sulfur dioxide and heavy metal would remain and cause a serious influence on quality and future development of TCM. This article reviews the chemical compositions change after SF to study the change law and their influence on quality. This article provide references for SF in TCMs' processing for a better and safer quality.
Drug Contamination
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Fumigation
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methods
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Medicine, Chinese Traditional
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methods
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Quality Control
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Sulfur
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chemistry
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Technology, Pharmaceutical
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methods
4.Progress of sulfur fumigation and modern processing technology of Chinese traditional medicines.
Tu-Lin LU ; Xin SHAN ; Lin LI ; Chun-Qin MAO ; De JI ; Fang-Zhou YIN ; Yong-Ying LANG
China Journal of Chinese Materia Medica 2014;39(15):2791-2795
Infestation, moldy and other phenomenon in the processing and storage of Chinese herbal medicines is a problem that faced in the production of Chinese traditional medicine. The low productivity of traditional processing methods can not guarantee the quality of Chinese herbal medicines. Sulfur fumigation is the first choice of grassroots to process the Chinese herbal medicine with its low cost and easy operation. Sulfur fumigation can solve some problems in the processing and storage of Chinese herbal medicines, but modern pharmacological studies show that long-term use of Chinese traditional medicine which is fumigated by sulfur can cause some serious harm to human liver, kidney and other organs. This paper conducts a review about the application history of sulfur fumigation, its influence to the quality of Chinese herbal medicines as well as domestic and foreign limits to sulfur quantity, and a brief introduction of the status of modern processing technologies in the processing of food and some Chinese herbal medicines, the problems ex- isting in the Chinese herbal medicines processing, which can provide a reference basis for the further research, development and application of investigating alternative technologies of sulfur fumigation.
Fumigation
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adverse effects
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methods
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Medicine, Chinese Traditional
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methods
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Quality Control
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Social Control, Formal
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Sulfur
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chemistry
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Technology, Pharmaceutical
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methods
5.Application of cell block technology in pathologic diagnosis of hematolymphiod neoplasms.
Yuan SHI ; Qin HU ; Yang ZHOU ; Ying-yong HOU ; Lu-de SUN ; Hong-xian XIE ; Akesu SUJIE ; Yun-shan TAN
Chinese Journal of Pathology 2010;39(8):553-554
Adolescent
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Adult
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Aged
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Aged, 80 and over
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Ascites
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pathology
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Biopsy
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Biopsy, Fine-Needle
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Child
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Cytodiagnosis
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methods
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Diagnosis, Differential
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Female
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Humans
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Leukemia-Lymphoma, Adult T-Cell
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pathology
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Lymphoma, B-Cell
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pathology
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Lymphoma, Large B-Cell, Diffuse
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pathology
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Lymphoma, T-Cell
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pathology
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Male
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Middle Aged
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Pleural Effusion
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pathology
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Young Adult
6.Expression of DNA-PK in hepato- and cholangio-neoplasms and its significance.
Zi-jian YU ; Jian-guo SUI ; Ying-qin DING ; Zhen-shan CAO ; Ping-kun ZHOU ; De-chang WU
Chinese Journal of Hepatology 2004;12(11):652-655
OBJECTIVETo characterize DNA-PKcs and Ku70 expressions in hepato- and cholangio-neoplastic tissues and the association with the degree of malignancy and invasiveness of the tumors.
METHODSThe expression of DNA-PKcs and Ku70 was examined in 47 cases of hepato- or cholangio-neoplasm by immunohistochemistry.
RESULTSKu70 was expressed in all of the neoplastic tissues examined and with a little variation in levels. The highest expression was observed in adenocarcinomas and adenomas. There was no statistically significant association between Ku70 expression level and the degree of their malignancy extent or invasiveness. In contrast to Ku 70, a wide variation in expression levels of DNA-Pkcs was observed among different types of neoplastic tissues. The highest ratio of positive expressing cells was detected in hepatocellular carcinomas (92.1%), which was significantly higher than that in cholangioadeno carcinomas (65.3%) and biliary cystadenocarcinomas (51.9%). Low or no expression level was detected in papillary adenoma cases. DNA-PKcs expression of invasive adenomas and adeno-carcinomas (61.2%) was significantly higher than that of non-invasive adenomas and adeno-carcinomas (30.4%). There was no expression observed in the normal tissues adjacent to the tumors.
CONCLUSIONDNA-PKcs is expressed in hepato- and cholangio-neoplasms and its variable level of expression is associated with the types of the tumor and their degree of malignancy and invasiveness. DNA-PKcs could be recognized as a new biomarker for liver neoplasm.
Adenocarcinoma ; enzymology ; Adolescent ; Adult ; Aged ; Aged, 80 and over ; Antigens, Nuclear ; biosynthesis ; genetics ; Bile Duct Neoplasms ; enzymology ; Bile Ducts, Intrahepatic ; enzymology ; Biomarkers, Tumor ; biosynthesis ; genetics ; Carcinoma, Hepatocellular ; enzymology ; DNA-Activated Protein Kinase ; biosynthesis ; genetics ; DNA-Binding Proteins ; biosynthesis ; genetics ; Female ; Humans ; Ku Autoantigen ; Liver Neoplasms ; enzymology ; Male ; Middle Aged
8.The ion targets of arrhythmias induced by ouabain and aconitine in guinea pig and rat ventricular myocytes.
Dong-mei GONG ; Hong-li SHAN ; Yu-hong ZHOU ; De-li DONG ; Bao-feng YANG
Acta Pharmaceutica Sinica 2004;39(5):328-332
AIMTo observe the effects of ouabain and aconitine on APD and ion channels in isolated guinea pig and rat ventricular myocytes; to elucidate the action mechanisms of these two drugs and set up new arrhythmic models on cellular level.
METHODSIn isolated ventricular myocytes of guinea pig and rat, the effects of ouabain and aconitine on APD, ICa-L, Ik, Ito and Ik1 were observed using the whole cell patch clamp technique.
RESULTSOuabain (5 micromol x L(-1)) obviously prolonged the APD90, increased ICa-L, decreased Ik and Ik1 in guinea pig ventricular myocytes. Aconitine (1 micromol x L(-1)) lengthened the APD90, increased ICa-L, decreased Ito and increased Ik1 in rat ventricular myocytes.
CONCLUSIONThe targets on ouabain- and aconitine-induced arrhythmias included APD, ICa-L, Ik, Ito, and Ik1. APD, ICaL, Ik and Ito must be the powerful ones, both in arrhythmic and antiarrhythmic courses. The ouabain- and aconitine- induced arrhythmic models on cellular level were built to study the antiarrhythmic mechanisms of chemicals and evaluate new drugs. These two new-type models in vitro were stable, liable, repeatable and economic, which were superior to those typical models in vivo.
Aconitine ; pharmacology ; Action Potentials ; drug effects ; Animals ; Arrhythmias, Cardiac ; pathology ; physiopathology ; Calcium Channels, L-Type ; drug effects ; Cell Separation ; Female ; Guinea Pigs ; Heart Ventricles ; pathology ; Male ; Myocytes, Cardiac ; metabolism ; physiology ; Ouabain ; pharmacology ; Potassium Channels, Inwardly Rectifying ; drug effects ; Rats ; Rats, Wistar
9.Dynamic expression of matrix metalloproteinase-2, membrane type-matrix metalloproteinase-2 in experimental hepatic fibrosis and its reversal in rat.
Zhi-hai ZHAO ; Shao-jie XIN ; Jing-min ZHAO ; Song-shan WANG ; Ping LIU ; Tie-yong YIN ; Guang-de ZHOU
Chinese Journal of Experimental and Clinical Virology 2004;18(4):328-331
OBJECTIVETo investigate the expression dynamics and significance of matrix metalloproteinase-2 (MMP-2) membrane type-matrix metalloproteinase-2 (MT-MMP-2) in hepatic fibrosis and its reversal counterpart.
METHODSAn experimental CCl4 induced hepatic fibrosis rat model was established by intraperitoneal administration of carbon tetrachloride for 2, 4, 6, 8, 10 weeks, and normal rats were used as a control group. The immunohistochemical methods and in situ hybridization were used to detect MMP-2,MT-MMP-2 mRNA and related antigens in the liver.
RESULTSMMP-2,MT-MMP-2 mRNA and related antigens were expressed in mesenchymal cells and parts of hepatocytes besides active pathological changes, especially in the fibrous septum and portal area. Expression of MMP-2,MT-MMP-2 mRNA and related antigens were increased in hepatic fibrosis and decreased gradually in its reversal counterpart.
CONCLUSIONThis study suggested that mesenchymal cells are the main cellular origins of MMPs. The levels of MMP-2 and MT-MMP-2 antigens and gene expression were closely related to hepatic fibrosis. MMP-2 and MT-MMP-2 may play important roles in hepatic fibrosis and its reversal counterpart.
Animals ; Carbon Tetrachloride Poisoning ; Gene Expression Regulation, Enzymologic ; Hepatocytes ; enzymology ; Liver ; enzymology ; pathology ; Liver Cirrhosis, Experimental ; enzymology ; etiology ; pathology ; Male ; Matrix Metalloproteinase 2 ; biosynthesis ; genetics ; Matrix Metalloproteinases ; biosynthesis ; genetics ; Matrix Metalloproteinases, Membrane-Associated ; Mesenchymal Stromal Cells ; enzymology ; RNA, Messenger ; biosynthesis ; genetics ; Rats ; Rats, Wistar
10.The curative effect of Fuzhengxiaojia decoction on precancerous lesions of gastric cancer
Tao SUN ; De-Shan LIU ; Yu ZHU ; Lan ZHOU ; Chang-Ling LI ; Shi-Qing YAO ; Chao WANG ; Li CHEN
Journal of Xi'an Jiaotong University(Medical Sciences) 2018;39(4):597-601,608
Objective To observe the curative effect of Fuzhengxiaojia decoction on precancerous lesion of gastric cancer (PLGC).Methods We randomly divided 44 patients with PLGC in our hospital into control group (n=22)and treatment group (n=22).The control group was given 4 Weifuchun tablets each time and three times per day and while the treatment group was given one Fuzhengxiaojia decoction of 400 mL besides the medication of the control group.They were treated for two courses,one course lasting for one month.Results Superoxide dismutase (SOD),malondialdehyde (MDA),glutathione peroxidase (GSH-Px),IgG,IgM and IgA in the two groups had no significant differences before treatment (P>0.05).After treatment,compared with those in the control group,SOD (t=2.144,P=0.044)and GSH-Px (t=2.322,P=0.030)increased,while MDA(t=3.096, P=0.005),IgG(t=2.421,P=0.025),IgM(t=3.377,P=0.003)and IgA (t=2.521,P=0.020)decreased. The main symptom scores in the two groups did not significantly differ before treatment (P<0.05).After treatment, compared with those in the control group,the scores for main symptoms like reduced food intake (t=3.924,P<0.001),stomach noise (t=4.161,P<0.001)and gastric or hypochondriac swelling (t=2.881,P<0.009) decreased in the treatment group.The rate of effective cases was higher than that in the control group (χ2=4.539, P=0.033).Conclusion The effect of Weifuchun combined with Fuzhengxiaojia prescriptions in treating PLGC is better than Weifuchun alone,which is related to improving redox and immunoglobulin.