Acute Disease ; Adult ; Arsenic Poisoning ; pathology ; Humans ; Male ; Respiratory System ; pathology ; Young Adult

Adolescent ; Adult ; Anti-Mullerian Hormone ; Biomarkers ; blood ; Child ; Child, Preschool ; Glycoproteins ; blood ; Humans ; Infant ; Infant, Newborn ; Male ; Testicular Hormones ; blood ; Testis ; growth & development

Objective:To study whether the orientation behavior of rats is affected by multi-sensory information.Methods: SD rats were randomly divided into 2 groups:the audio-visual integration group and pure audio group.The orientation behaviors of rats were observed after given single modal and multi-modal sensory cues with spatial disparities.Results:We found that when the temporal and spatial audio cues were coincident to a target visual cue,the rats had a markedly reduced reaction time and increased success rate in orientation behaviors;there was an obvious audio-visual integration effect.The integration effect was more obvious when the intensity of target visual cue was weak,with the enhancement efficiency being(87.3?8.5)%, significantly higher than that when the intensity of target visual cue was stronger(27.6%,P

Abdominal Neoplasms ; complications ; metabolism ; pathology ; surgery ; Adult ; Dendritic Cell Sarcoma, Follicular ; complications ; metabolism ; pathology ; surgery ; Diagnosis, Differential ; Female ; Humans ; Ki-1 Antigen ; metabolism ; Leukocytosis ; complications ; metabolism ; pathology ; surgery ; Receptors, Complement 3b ; metabolism ; Receptors, Complement 3d ; metabolism ; Young Adult

OBJECTIVETo prepare semiconductor quantum dots (QDs)-Smad2 monoclonal antibody fluorescent probes, to detect the optical qualities and the ability to specific recognition of Smad2 in rat dental papillae cells (RDPC) of quantum dots-Smad2 monoclonal antibody fluorescent probes.

METHODS(1) QDs were chemically modified with Smad2 proteins to prepare water soluble QDs-Smad2 monoclonal antibody fluorescent probes which were purified after preparation. (2) The absorption band and emission band of these probes were obtained through ultraviolet spectrophotometer and fluorospectrophotometer, the shape, fluorescence intensity and photochemistry stability of these probes were studied through confocal laser scanning fluorescence microscopy. (3) Before the location of Smad2 proteins in RDPC was studied with anti-Smad2 immunocytochemical method and direct immunofluorescence imaging, RDPC were incubated with transforming growth factor-beta1 (TGF-beta1), and the related optical qualities of quantum dots-Smad2 monoclonal antibody fluorescent probes in RDPC were detected.

RESULTSQDs and monoclonal antibody linked together through covalent bond to form the fluorescent probes which could specifically and effectively recognize Smad2 proteins in RDPC. These fluorescent probes still had good properties, including broad excite spectra, narrow emission spectra, high fluorescence intensity and photostability.

CONCLUSIONQDs and monoclonal antibody could link together through covalent bond to form the nanometer molecular probes with distinct optics character and photostability, which provides the scientific evidence that QDs can visualize the molecular movement in living cells in long-term, in situ and in real time.

Animals ; Antibodies, Monoclonal ; Fluorescent Dyes ; Quantum Dots ; Rats ; Semiconductors ; Smad2 Protein

Animals ; Astrocytes ; immunology ; metabolism ; Male ; Microglia ; immunology ; metabolism ; Neurons ; metabolism ; Oxidopamine ; metabolism ; Parkinson Disease ; immunology ; Rats ; Rats, Sprague-Dawley

Objective To prepare semiconductor quantum dots (SQD)-Smad4 monoclonal antibedyfluorescent probes and to detect the optical qualities and the ability of specific recognition of the probes. Methods SQD were chemically modified with Smad4 monoclonal antibody proteins to prepare water soluble probes, and the fluorescence intensity, photostability, absorption spectra and emission spectra of the probes were studied. The location of Smad4 in rat dental papillae cells (RDPC) was examined by SP anti-Smad4 immunocytochemical method and SQD-Smad4 direct immunofluorescent imaging. Results SQD and monoclonal antibody covalently bonded to form the fluorescent probes which could specifically recognize Smad4 in RDPC. These fluorescent probes still had properties, including broad absorption band, narrow emission band, high fluorescence intensity and photostability. Conclusions SQD and monoclonal antibody could eovalently bond to form the fluorescent probes with distinct optics character and ability of specificrecognization, which provides the scientific evidence that SQD trace the molecular movement in living cells inlong-term, in situ and in real time.

OBJECTIVETo explore the efficacy and determine the risk factors of survival for recurrent hepatocellular carcinoma(HCC) treated by percutaneous radiofrequency ablation (PRFA).

METHODSFrom January 1999 to December 2008, 82 patients with recurrent HCC, with the diameter less than 7 cm for solitary tumor, or the largest tumor less than 5 cm for multiple tumors(the number of tumors less than 3), were treated by PRFA. The significance of 12 clinical or pathological variables in the risk factors of overall survival were assessed.

RESULTSThe overall survival 1-, 3-, and 5-year survival rates were 75.8%, 43.9% and 34.5% (from the date of PRFA), and 95.1%, 63.2% and 46.6% (from initial hepatectomy), respectively. Univariate analysis indicated that tumor size before initial hepatectomy, recurrence interval from initial hepatectomy, number of recurrent tumors, diameter of largest recurrent tumor, serum glutamyl transpeptidase (GGT) and serum albumin (ALB) level were significant prognostic factors (P < 0.05, Kaplan-Meier Log-rank test). Multivariate analysis showed recurrence interval from initial hepatectomy, diameter of largest recurrence tumor, serum GGT and ALB level were significant prognostic (P < 0.05).

CONCLUSIONPRFA is effective for recurrent HCC. Recurrence interval from initial hepatectomy, diameter of largest recurrent tumor, serum GGT and ALB level are significant prognostic factors.

Adult ; Carcinoma, Hepatocellular ; pathology ; surgery ; Catheter Ablation ; Female ; Follow-Up Studies ; Humans ; Kaplan-Meier Estimate ; Liver Neoplasms ; pathology ; surgery ; Male ; Middle Aged ; Neoplasm Recurrence, Local ; surgery ; Prognosis ; Retrospective Studies ; Risk Factors

AIMTo further explore the roles of endogenous nitric oxide (NO) or NO derivatives in complex partial seizures and generalized convulsions.

METHODSThe effect of pretreatment with L-nitroarginine (L-NNA), an inhibitor of nitric oxide synthase (NOS), or L arginine (L-Arg), a precursor of NO on kainic acid (KA)-induced seizure in rats and the changes in the concentration of NO2 -/NO- in the hippocampus were determined.

RESULTSThe rats appeared with wet dog shakes (WDS) at 15 min and then occurred generalized convulsions during 1 h to 3 h after administration of KA (10 mg/kg i.p.). However, the pretreatment of L-NNA (50 mg/kg) so dramatically promoted and enhanced KA-induced behavioral seizures that the latency of generalized convulsion was shorten dramatically, and the mortality was greatly high. In contrast, the pretreatment with L-Arg (40 mg/kg) markedly delayed or weakened KA-induced behavioral changes, such as increasing latency of WDS and generalized convulsion, shortening time o f seizure and none of animal died during observed time. The concentration of NO2- /NO3- in the hippocampus increased immediately at 30 min and remained to 7 d after the administration of KA. Compared with control group (pretreatment with NS), the concentration of NO2- / NO3- in the hippocampus apparently increased at 3 h and 3 d after the administration of KA in the rats with L-Arg pretreatment.

CONCLUSIONThe endogenous NO (NO or NO derivatives) mediators may play an important role against excitotoxin induced seizures in rats.

Animals ; Arginine ; pharmacology ; Kainic Acid ; adverse effects ; Male ; Nitric Oxide ; metabolism ; Nitroarginine ; pharmacology ; Rats ; Rats, Wistar ; Seizures ; chemically induced ; metabolism

OBJECTIVEObserving the time course and establishing the model of kappaB-decoy oligodeoxynucleotides (rcB-decoy) inhibiting the activity of NF-kappaB in the PC12 cells.

METHODSPC12 cells cultivating in the 6 wells plate were divided into 3 groups, experimental group: adding kappaB-decoy complex (6 microg DNA/well), the control group: adding scrambled-decoy complex, the normal group: adding lipid-Lipofectamine 2000, transfer and cultivate 48 h, then lipopolysaccharide (LPS, 200 ng/ml) was added in the cells for 0.5-4 h. The immunocytochemistry and Western blot were used to measure the expression or the activity of NF-kappaB in PC12 cells.

RESULTSIn PC12 cells, compared with normal group, the expression of NF-kappaB enhanced obviously with the time of the stimulation of LPS in scrambled-decoy treated control group (P < 0.01), in 2-4 h the level reached the peak; the expression of NF-kappaB showed the stable level with the time of the stimulation of LPS in kappaB-decoy treated experimental group, compared with the control group, the expression levels were obviously lower than the respective time point of control groups (P < 0.01).

CONCLUSIONkappaB-decoy could reduce the expression of NF-kappaB in the normal PC12 cells and inhibit the activity of NF-cB in the pathologic PC12 cells.

Animals ; Cells, Cultured ; Lipopolysaccharides ; pharmacology ; NF-kappa B ; antagonists & inhibitors ; metabolism ; Oligodeoxyribonucleotides ; pharmacology ; PC12 Cells ; Rats