1.Existant quality problems and suggestions for quality control of medical absorbent cotton.
De-long JIANG ; An-zheng CHI ; Qi-hua ZHANG
Chinese Journal of Medical Instrumentation 2005;29(5):364-365
In the process of acid hydrolysis of medical absorbent cotton, we have discovered that some of the domestic manufactured medical absorbent cotton is mixed with wasted non-cotton chemical fiber. If the cotton is used in medical treatments, the chemical fiber will cause irritation, allergy and inflammation, so it's very harmful. But the non-cotton fiber content is not stipulated in the standard of YY0330-2002, and no testing method for it is available. In this paper we discuss the existent quality problems, the control and the test method for non-cotton chemical fiber in medical absorbent cotton.
Bandages
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standards
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Cotton Fiber
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Quality Control
2.Bacteria Composition of Organic Matter-decomposing Inoculant Analyzed with 16S rDNA Clone Library
Guo-Yuan LI ; Jun LI ; Xin JIANG ; Li LI ; De-Long SHEN ;
Microbiology 1992;0(05):-
Using 16S rDNA clone library method, the bacteria composition of the Organic Matter-decomposing Inoculants A and B were investigated. The results indicated that: Sample A was clustered into 14 taxonomic operational units (OTUs),the dominant communities are Weissella confuse, Bacillus subtilis and Bacillus pumilus, which accounted for 28.6%,30.4% and 23.2%;Sample B was clustered into 43 OTUs,the dominant communities are Lactobacillus buchneri, Lactobacillus farciminis and Lactobacillus acetotolerans, which accounted for 18.03%,18.86% and 13.12%, respectively. The results had much difference from the samples' labels:there were not bacteria in the lable of sample A and only Bacillus pumilus was the same with the lable of sample B.This study showed that 16S rDNA clone library method has nicer application perspectives in analying the bacteria of microbial inoculant and its quality control.
3.Diversity of Endophtic Bacteria Isolated from Glycyrrhiza
Min ZHANG ; De-Long SHEN ; Xiao-Li RAO ; Feng-Ming CAO ; Xin JIANG ; Jun LI ;
Microbiology 1992;0(04):-
120 strains of endophytic bacteria identified by ERIC-PCR were isolated from wild and cultivated Glycyrrhiza uralensis plants which collected from Erdos Innermongolia province.The identified results indicated that Glycyrrhiza uralensis plants has plenty of endophytic bacterium in density and population,and the density is higher in root and leave than in stem.Partial sequence analysis of 16S rDNA gene of 82 strains indicated that these strains were in a high similarity with 19 known genus which belong to?、?、 ?-Proteobacteria、Firmicutes and Actinobacteria.The dominant genus were Bacillus sp.,Pseudomonas sp., Pantoea sp.and Serratia sp..
4.Treatment of portal vein tumor thrombosis in patients with hepatocellular carcinoma by TACE
Zheng-Long TAO ; Yu-Feng JI ; Hong-Xian JIN ; Ying-Jiang ZHAN ; Yong-De CHEN ;
Journal of Interventional Radiology 2006;0(11):-
Objective To evaluate the theraputic effect of transcatheter arterial chemoembolization (TACE)for hepatocellular carcinoma with tumor thrombosis of portal vein.Methods One hundred and six patients of hepatocellular carcinoma with tumor thrombosis of portal vein under treament of TACE were observed before and after the procedure.Results After TACE tumor size reduced>50% in 23 patients,<50% in 25, no significant change in 44.The size of tumor enlarged in 12.The disappearance of portal vein tumor thrombosis accessed in 14,with reduction in 39,and no significant change in 51.Two patients died within one week.Conclusion TACE provides good therapeutic effect on hepatocellular carcinoma with tumor thrombosis of portal vein.
5.Observation and contrast of anaerobe culturing result using three kinds of device
Chang-Fa SHAO ; De-Ren LI ; Yun-Long YANG ; Ken-Ying JIANG ;
Chinese Medical Equipment Journal 1993;0(05):-
The positive ratio is 74 percent using the plate anaerobe culturing device made by author, higher than using common anaerobic box and anaerobic jar.
7.Isolation of Endophytic Bacteria from Glycyrrhiza and Identifying of Antagonistic Bacteria
Xiao-Li RAO ; De-Long SHEN ; Jun LI ; Xin JIANG ; Li LI ; Min ZHANG ; Rui-Hua FENG ;
Microbiology 1992;0(04):-
98 endophytic bacteria strains were isolated from different internal tissues of Glycyrrhiza uralensis plants collected from Innermongolia region. Results indicated that the population densities of endophytic bacteria ranged from 5.0?104cfu/g~2.9?107cfu/g fresh weight although it varied depending on tissue of the plant. Among these strains, Bacillus sp. was the most prevalent endophytic bacterium, which was amount to 30%.Of the 98 isolates, 6 strains exhibited extensive antagonistic activities against pathogenic bacteria. Characterization showed that these bacteria were Bacillus atrophaeus、Paenibacillus polymyxa、Bacillus subtilis、Paenibacillus ehimensis. This study indicated that selected 6 endophytic bacteria strains have potential for biological control of plant disease.
8.Study on the mutations of ATP2C1 gene in patients with Harley-Harley disease
Xiao-Xiao YAN ; De-Ke JIANG ; Fu-Ren ZHANG ; Hong-Qing TIAN ; Gui-Zhi ZHOU ; Xian-Mei LU ; Long YU
Chinese Journal of Dermatology 1994;0(06):-
Objective To detect the mutations of ATP2C1 gene in patients with Hailey-Hailey dis- ease (HHD).Methods PCR and direct sequencing were performed in 17 patients and 120 healthy controls to screen the mutations in the exons of ATP2C1 gene.Results Eight mutations were identified in nine probands, including three deletion mutations (nt1464-1487 del/nt1462-1485del,1523delAT,2375delTTGT),three splice site mutations (360—2A→G,1415—2A→T,2243+2T→C) and two missence mutations (C920T and G1942T).None of the above mutations was found in the controls.Conclusion Eight specific novel mutations were identified in nine probands of HHD,which could be causative factors of the disease.
9.Splenic hamartoma: case report and review of literature.
Hong-bo JIA ; Ying-ping LI ; De-en HAN ; Yao LIU ; Bin ZHANG ; De-quan WU ; Xi CHEN ; Ying JIANG ; Long-xian ZHENG ; Jin-rong DU ; Xue-hai JIANG
Chinese Medical Journal 2006;119(16):1403-1408
Adult
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Female
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Hamartoma
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complications
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diagnosis
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surgery
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Humans
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Kidney Calculi
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complications
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Spleen
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pathology
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surgery
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Splenectomy
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methods
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Splenic Diseases
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complications
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diagnosis
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surgery
10.Determination of the concentrations of interleukin-18 and other cytokines in the synovial fluid in patients with osteoarthritis.
Yong LI ; Jian-ming JIANG ; De-hong YANG ; Feng-long WANG ; Zhong-xuan MAO
Journal of Southern Medical University 2009;29(4):729-731
OBJECTIVETo determine the concentrations of interleukin-18 (IL-18), IL-6, IL-8, and prostaglandin E2 (PGE2) in the synovial fluid in patients with osteoarthritis (OA), and explore the role of IL-18 in the pathogenesis of OA.
METHODSThe synovial fluid was collected from 30 patients with knee OA, and the concentrations of IL-18 and the other cytokines were measured using enzyme-linked immunosorbent assay (ELISA). A linear regression was performed between IL-18 and the other cytokines.
RESULTSThe average IL-18 and PGE2 concentrations were 220-/+304 pg/ml and 89-/+104 pg/ml in the synovial fluid, respectively, and the two cytokines showed a positive correlation in the synovial fluid (r=0.628, P=0.001). The IL-18 concentration was also correlated to the concentrations of IL-6 (1200-/+1587 pg/ml, n=22; r=0.590, P=0.008) and IL-8 (5190-/+6024 pg/ml, n=9; r=0.776, P=0.014).
CONCLUSIONIL-18 can promote PGE2 production, which causes cartilage degradation in OA, thus therapies targeting this cytokine may prove an effective approach to early OA treatment.
Aged ; Dinoprostone ; biosynthesis ; Female ; Humans ; Interleukin-18 ; metabolism ; Male ; Middle Aged ; Osteoarthritis ; metabolism ; Synovial Fluid ; metabolism