Acupuncture ; history ; Acupuncture Points ; China ; History, Ancient ; Humans ; Medicine in Literature ; Terminology as Topic

Biopsy ; Cryopreservation ; Frozen Sections ; Humans ; Kidney ; pathology ; Kidney Diseases ; pathology ; Paraffin Embedding ; methods

Aged ; Female ; Humans ; Male ; Middle Aged ; Pharyngeal Diseases ; pathology ; Polyps ; pathology

Objective To investigate the dynamic changes of the spinal cord during neck flexion in Hirayama disease for diagnosis.Methods MRI examinations in neutral neck position and a fully flexed neck position were performed on 18 cases of Hirayama disease and 31 young normal control subjects.We measured an antero-posterior diameter(APD)and transverse diameter(TD)of the cervical cord at the superior margin of the C6 vertebral body for each position,and investigate the dynamic changes.The different in frequency of these findings between the control and patient groups was examined by means of the x~2 test.The group means were compared by independent-sample t-test.Significance was defined as P

To investigate the absorption kinetics of Cu B-SDC/PLC-MMs in rat different intestinal segments and compared with the absorption of Cu B suspension. The in vitro everted gut sacs model was established to study the absorption characteristics of Cu B-SDC/ PLC-MMs in rat duodenum, jejunum, ileum and colon, and the content of cucurbitacin B was detected by HPLC method, and the effects of concentrations on intestinal absorption were evaluated as well. The results showed that the absorption of Cu B-SDC/PLC-MMs was linearity at different intestine segment and different concentrations (R2 > 0.9), which was consistent with zero order rate process. The Ka of different intestine segments showed a concentration-dependent increasing along with the raised concentration of Cu B-SDC/ PLC-MMs, indicating that it was likely to be a mechanism of passive absorption. The best absorption site of Cu B-SDC/PLC-MMs was ileum, and its absorptions in different intestinal segments were superior to cucurbitacin B suspension. SDC/PLC-MMs could significantly enhance the intestinal absorption of cucurbitacin B, and the study of intestinal absorption kinetics of Cu B-SDC/PLC-MMs had gave a support to its further reasonable solidfication.
Animals ; Deoxycholic Acid ; administration & dosage ; Female ; Intestinal Absorption ; Kinetics ; Male ; Micelles ; Nanoparticles ; Phospholipids ; administration & dosage ; Rats ; Rats, Wistar ; Triterpenes ; administration & dosage ; pharmacokinetics

Adolescent ; Adult ; Cytokines ; blood ; Endotoxemia ; complications ; Female ; Hepatitis B, Chronic ; complications ; immunology ; Humans ; Male ; Middle Aged ; Th1 Cells ; immunology ; Th2 Cells ; immunology

OBJECTIVETo establish fingerprinting of Flos Buddleja by using RP-HPLC for the quality control.

METHODThe HPLC condition was as follows: Inertsil ODS-3 C18 analytical column (4.6 mm x 250 mm, 5 microm), gredient eluation with MeCN (0.1% TFA)-H2O (0.1%TFA), flow rate 1.0 mL x min(-1), detection wavelength 254 nm. 10 commercial samples were analyzed to establish a fingerprinting.

RESULTAmong the obtained fingerprinting, most of the detected peaks were separated effectively. The accuracy, repeatability and stability of this method were satisfied. The RSDs of relative retention time and area of aimed peaks which existed in all samples wereless than 5%. Theresults were in accordance with the request of fingerprinting.

CONCLUSIONThe established fingerprinting can be used for the quality control of Flos Buddleja.

Buddleja ; chemistry ; China ; Chromatography, High Pressure Liquid ; methods ; Ecosystem ; Flowers ; chemistry ; Plants, Medicinal ; chemistry ; Quality Control

Adolescent ; Apolipoproteins E ; blood ; Humans ; Hyperlipoproteinemias ; complications ; Hypolipidemic Agents ; therapeutic use ; Kidney Diseases ; blood ; drug therapy ; pathology ; Kidney Glomerulus ; blood supply ; pathology ; Male ; Thrombosis ; pathology

Abstract: Objective　To investigate the drug demand and related influencing factors of AIDS non-occupational post-exposure prophylaxis (nPEP) among men who have sex with men (MSM) in Wuhan, and to provide a scientific basis for the development of subsequent intervention policies for MSM. Methods　With the assistance of social organizations in Wuhan, MSM was recruited by the snowball method to carry out an online questionnaire survey to collect information on demographics, AIDS-related knowledge, high-risk behaviors, and the need for nPEP medication. The χ2 test and unconditional Logistic regression were used to analyze the related factors of the demand for nPEP medication. Results　A total of 308 valid subjects were included in this study, with predominantly 18-29 years old (78.57%, 242/308). The self-reported sexual orientation was mainly homosexuality (82.47%, 254/308), and the awareness rate of AIDS knowledge was high (89.29%, 275/308). Among the survey respondents, 35.06% (108/308) did not know the situation of HIV infection among MSM population in Wuhan; 55.19% (170/308) had two or more same-sex sexual partners in the last six months; 90.91% (280/308) had heard of nPEP before participating in this survey. After passing nPEP and informing the protective effect of nPEP, 59.42% (183/308) of them needed nPEP. After HIV exposure, 73.38% (226/308) were willing to spend money to buy nPEP drugs, and 88.64% (273/308) were willing to take nPEP drugs because of the known side effects. Logistic regression analysis showed that in the last six months, the needs for taking nPEP medication in those who had 2 or more same-sex sexual partners (OR=2.121, 95%CI: 1.329-3.386) and who had received peer education (OR=1.740, 95%CI: 1.088-2.781) were higher than those of those who had a same-sex sexual partner in the last six months and who had not received peer education. Conclusions　The MSM population in Wuhan has a great demand for nPEP drugs, and peer education is an important way to carry out nPEP publicity and promotion. At the same time, we should continue to strengthen warning publicity and education and behavioral intervention to reduce MSM risky sexual behaviors and reduce new HIV infections.

Objective To investigate the clinical efficacy of meloxicam combined with glucosamine sulfate in the treatment of patients with osteoarthritis.Methods Ninety cases of knee osteoarthritis were randomly divided into control group and treatment group,each group 45 cases.Control group was given meloxicam tablets 7.5 mg,bid,oral.On the basis of control group,treatment group was treated with glucosamine sulfate capsules 0.5 g,tid,oral.All the patients were treated for 6 weeks.The clinical symptoms,signs,knee joint motor function and pain indexes of all patients were evaluated before and after treatment.The blood samples and joint fluid specimens were collected,to determine the matrix metalloproteinase-3 (MMP-3),matrix metalloproteinase-13 (MMP-13) and prostaglandin E2 (PGE2) levels by enzyme linked immunosorbent assay (ELISA).The clinical efficacy and adverse drug reactions in two groups were compared.Results After treatment,the levels of Lequesne indexes in treatment group and control group were 5.78 ± 3..04 and 8.24 ± 3.62,Lysholm scores were 85.82 ± 10.01 and 72.46 ± 13.58,VAS scores were 2.45 ± 1.08 and 3.86 ± 1.17,serum MMP-3 levels were (114.69 ± 13.54) and (130.52 ± 16.48) ng · L-1,serum MMP-13 levels were (141.06 ± 21.55) and (179.82 ± 24.37) ng · L-1,serum PGE2 levels were(69.82 ± 18.67)and(91.30 ±20.09)ng · L-1,joint fluid MMP-3 levels were(17.89 ±5.12) and (23.10 ±5.46)ng · L-1,joint fluid MMP-13 levels were(27.35 ±6.52)and(38.47 ±6.85)ng · L-1,joint fluid PGE2 levels were (82.16 ± 22.64) and (118.02 ± 23.41) ng · L-1,all with significant difference (all P ＜ 0.05).The improvement rates of control group and treatment group were 73.33％ (33 cases/45 cases),91.11％ (41 cases/45 case),with significant difference (P ＜ 0.05).No adverse drug reactions occurred during the treatment.Conclusion The clinical effect of meloxicam combined with glucosamine sulfate in the treatment of knee osteoarthritis is exact,the treatment is conducive to the improvement of clinical symptoms,can reduce serum MMP-3,MMP-13,PGE2 levels,with high safety.