2.A Preliminary study on serum Anti—Human—Chromosome Antibodies
Xiao-Hui JI ; De-Hua KOU ; Yuan GU ; Shu-Yun YUAN ; Wenping DU ; Kaiti WU ; Yiwen RONG
Chinese Journal of Immunology 1985;0(05):-
With human chromosomes,as antigen,anti—human—chromosome antibodies (AhChrA)were detected specifically from SLE patients sera by the methods of immunogold—silver staining(IGSS)and immnuofluorescenee tese (IFT)。To SLE,the sensitivity and specificity of serumAhChrA was 58.1%and98.5%respeetively in IGSS,34.9%and99.5%respectively in IFT。Boththe incidence and titer of AhChrA were found to be colsely related to the pathoactivity and thedamages of some important organs or tissues,such as kidney damage,abnormal immunity andhematocytopenia.A preliminary analysis of the antigen components reacting to AhChrA was alsoperformed。
3.Identification of the structure of two new nitro group phenolic glycosides from Schisandra propinqua (Wall. ) Baill var. intermidia A. C. Smith.
Tong WU ; De-Yun KONG ; Hui-Ting LI
Acta Pharmaceutica Sinica 2004;39(7):534-537
AIMTo study the bioactive components from Schisandra propinqua (Wall.) Baill var. intermidia A. C. Smith.
METHODSCompounds were separated with a combination of multichromatography. Their chemical structures were determined on the basis of spectral analysis and chemical evidence.
RESULTSSeven compounds were isolated from the leaves of Helicia nilagirica. The structures were elucidated as 6'-O-alpha-L-arabinofuranosylthalictoside (1), 6'-O-beta-D-apiofuranosylthalictoside (2), thalictoside (3), icariside D2 (4), prinsepiol (5), (+)-1-hydroxypinoresinol (6) and (+)-medioresinol (7).
CONCLUSIONTwo compounds are new nitro phenolic glycosides.
Disaccharides ; chemistry ; isolation & purification ; Molecular Conformation ; Molecular Structure ; Nitro Compounds ; chemistry ; isolation & purification ; Plant Stems ; chemistry ; Plants, Medicinal ; chemistry ; Schisandra ; chemistry
4.Structure identification of two new cerebrosides from Helicia nilagirica Beed.
Tong WU ; De-Yun KONG ; Hui-Ting LI
Acta Pharmaceutica Sinica 2004;39(7):525-527
AIMTo study the bioactive components from Helicia nilagirica.
METHODSCompounds were separated with a combination of multi-chromatography. Their chemical structures were determined on the basis of spectral analysis and chemical evidence.
RESULTSTwo compounds were isolated from the leaves of Helicia nilagirica. Compound 1 was elucidated as 1-O-3-D-glucopyranosyl-(2S,3S,4R,8Z)-2-[(2'R)-2'-hyd roxylignocenoyl-amino]-8-octadecene-1, 3, 4-triol. Compound 2 was an analogue of 1.
CONCLUSIONThe two compounds are new cerebrosides.
Cerebrosides ; chemistry ; isolation & purification ; Glucosylceramides ; chemistry ; isolation & purification ; Molecular Conformation ; Molecular Structure ; Plant Leaves ; chemistry ; Plants, Medicinal ; chemistry ; Proteaceae ; chemistry
5.Isolation,Identification and Degradation Characteristics of a DMP-degrading Strain
De-Cai JIN ; Xue-Ling WU ; Ren-Xing LIANG ; Qin-Yun DAI ; Yang-Yang WANG ; Yu YANG ;
Microbiology 2008;0(09):-
A bacterial strain which could grow well on the substrate of PAEs as the sole source of carbon and energy was isolated from contaminated sludge in the river of WeiFang in ShangDong province and it was designated as JDC-3. Based on the morphology,biophysical and biochemical properties as well as molecular characteristics,this isolate was preliminarily identified as Delftia sp.. A fragment of phthalate dioxygenase gene was successfully amplified from the genus of Delftia for the first time using a set of degenerate primers. Meanwhile,the degradation capability of JDC-3 was determined by HPLC using DMP as test substrate. The results showed that the optimal pH and temperature were at 7.0~8.0 and 30?C~35?C respectively. The degradation kinetics of JDC-3 was studied in different initial DMP concentration under optimal conditions. The results indicated that the degradation dynamic equation was ln C =-0.06837 t + A when DMP concentration was lower than 300 mg/L,with half life of 12.48 h. The degradation rate decreased and half life of JDC-3 prolonged as the initial concentration kept on increasing.
6.Mechanism of improving effect of losartan on insulin sensitivity of non-insulin-dependent diabetes mellitus rats.
Yong WU ; Jing-Ping OUYANG ; Yun-Feng ZHOU ; Ke WU ; De-Hai ZHAO ; Chong-Yuan WEN
Acta Physiologica Sinica 2004;56(4):539-549
The specific inhibition of angiotensin II action at AT(1) receptors by losartan has been shown to decrease peripheral insulin resistance in type 2 diabetic patients and animal models. We examined the effect of losartan on the expression of insulin receptor substrate 1 (IRS-1), protein kinase B (PKB) and glucose transporter 4 (GLUT4), as well as the phosphorylation status of IRS-1 and the association between IRS-1 and phosphatidylinositol (PI) 3-kinase in skeletal muscle from fat-fed and-streptozotocin (STZ)-treated rats, an animal model of type 2 diabetes mellitus. In addition, the effects of losartan on GLUT4 translocation in muscle cells and on insulin sensitivity were also evaluated. Muscle tissues were isolated from male losartan-treated and untreated normal or non-insulin-dependent diabetes mellitus (NIDDM) rats with a dose of 4 mg/kg per day for 6 weeks. Oral administration of losartan improved insulin sensitivity, which was determined by an oral glucose tolerance test (OGTT). In skeletal muscles, the protein levels of IRS-1, PKB and GLUT4 in NIDDM rats were not significantly different from those of the control rats, and they were not affected by losartan. The levels of IRS-1 tyrosine phosphorylation, PI 3-kinase activity associated with IRS-1 and PKB activation after stimulation with insulin in muscle tissue of NIDDM rats were significantly decreased (P<0.01) compared with those in the control rats, while they were not increased by losartan. Losartan had a major effect on GLUT4 translocation in myocytes, as it significantly increased (P<0.05) the insulin-induced amounts of GLUT4 in plasma membrane (PM) and T-tubules (TT) in myocytes from NIDDM rats. Consistent with these results, the plasma glucose level in losartan-treated NIDDM rats was decreased (P<0.05) compared with that in untreated NIDDM rats. Our results suggest that losartan may exert beneficial effects on insulin resistance by increasing the translocation of GLUT4 in muscle tissue, which is probably associated with a non-PI 3-kinase-dependent mechanism.
Animals
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Diabetes Mellitus, Experimental
;
blood
;
drug therapy
;
Diabetes Mellitus, Type 2
;
blood
;
drug therapy
;
physiopathology
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Glucose Transporter Type 4
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Insulin Receptor Substrate Proteins
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Insulin Resistance
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Losartan
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pharmacology
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therapeutic use
;
Male
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Monosaccharide Transport Proteins
;
biosynthesis
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genetics
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Muscle Proteins
;
biosynthesis
;
genetics
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Muscle, Skeletal
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metabolism
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Phosphoproteins
;
biosynthesis
;
genetics
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Protein-Serine-Threonine Kinases
;
biosynthesis
;
genetics
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Proto-Oncogene Proteins
;
biosynthesis
;
genetics
;
Proto-Oncogene Proteins c-akt
;
Rats
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Rats, Sprague-Dawley
7.The effect of high mobility group box-1 in endotoxin-induced acute hepatic failure.
Zhong-fu ZHAO ; De-wu HAN ; Yun ZHANG ; Feng WANG ; Ming-she LIU
Chinese Journal of Hepatology 2006;14(5):388-389
Animals
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Endotoxins
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Female
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HMGB1 Protein
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biosynthesis
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Liver Failure, Acute
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chemically induced
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metabolism
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Male
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Rats
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Rats, Wistar
8.Treatment of rheumatoid arthritis with T-614:a multicenter,randomized,double blind,placebo-controlled trial
Jia-Lin TENG ; Liang-Jing LV ; Chun-De BAO ; Xing-Hai HAN ; Ling-Yun SUN ; Jian-Hua XU ; Xing-Fu LI ; Hua-Xiang WU ;
Chinese Journal of Rheumatology 2003;0(08):-
Objective To study the efficacy and safety of T-614 in treating rheumatoid arthritis(RA). Methods Two hundred and eighty patients with active RA were randomly allocated to 3 groups:T-614 50 mg each day,25 mg each day or placebo.Clinical and laboratory parameters were analyzed at baseline,2,4,6,12, 18 and 24 weeks.Results The ACR response rate was significantly higher in the T-614 treatment group com- pared with the placebo group during the first 6 weeks.After 24 weeks,25 mg/d,50 mg/d dosage group and the placebo group showed 39.1%,61.3% and 24.2% in ACR20,23.9%,31.2% and 7.4% in ACR50 respectively.A time-response in ACR response after 24 weeks was observed,with clear superiority of the 25 mg/d and 50 mg/d dosage groups compared to the placebo,and 50 mg/d dosage group compared to 25 mg/d dosage group(P
9.Concentration of Ca2+, contents of cAMP, CaM and Ca2+/CaM-PK II in pheochro-mocytoma PC12 cells after combined soman and hypoxia injury
Ji-Qing ZHAO ; Qiang WU ; Shi-Li WANG ; Xiang-De WEI ; Zhao-Jun DONG ; Yun-Peng LI ; Yong LIU
Journal of Third Military Medical University 2001;23(2):169-171
Objective To observe the changes of the concentration of Ca2+, contents of cAMP, CaM and activity of Ca2+/CaM-PK II in pheochromocytoma PC12 cells after combined soman and hypoxia injury. Methods The changes of [Ca2+], and activity of CaM, cAMP and Ca2+/CaM-PK II in PC12 cells were studied after combined soman and hypoxia injury with radioimmunoassay. Results The changes of [Ca2+], the contents of CaM, cAMP were significantly higher in hypoxic and soman intoxicated group than in soman intoxicated group and control group under hypoxia; but the activity of Ca2+/CaM-PK Ⅱ were significantly decreased. Conclusion [Ca2+], CaM, cAMP and Ca2+/CaM-PK Ⅱ exert important role in the damage of PC12 after combined soman and hypoxia injury.
10.Repairing large alar defects by using a retro-grade auricular flap.
Fei FAN ; Zong-ji CHEN ; Chuan-de ZHOU ; Nian WU ; Yun-chun MAO ; Lian-zao WANG ; Gang ZHOU
Chinese Journal of Plastic Surgery 2003;19(2):107-109
OBJECTIVETo evaluate a retro-grade auricular flap for repairing large alar defects.
METHODSTwenty-nine adult cadavers were anatomically used for vascular investigation of the frontal, nasal and temporal regions by injecting a dye into the main vessels. Based on the anatomical study in these regions, a retro-grade auricular flap was designed for repairing alar defects in 16 patients.
RESULTSThe blood supply of the auricle could be nourished by the inner carotid artery system from the supratrachlear artery and supraorbital artery through the frontal arterial anastomotical network into the frontal branch of the superficial temporal artery. It is then passing the main trunk of the superficial temporal artery into the ear area through the auricular branches of the superficial temporal artery. The retro-grade auricular island flap could be formed by basing the supratrachlear artery and the supraorbital artery through the vascular network between the superficial temporal artery and the supratrachlear artery or the supraorbital artery. Sixteen patients with large alar defects and half-sized nasal defects were successfully repaired by this technique.
CONCLUSIONSThe retro-grade auricular island flap, based on the inner carotid artery system, could be a good and safe flap for repairing a large alar defect or half-sized nasal reconstruction.
Adult ; Arteries ; Cadaver ; Carotid Artery, Internal ; Ear Auricle ; blood supply ; Humans ; Nose Deformities, Acquired ; surgery ; Ophthalmic Artery ; Reconstructive Surgical Procedures ; Surgical Flaps ; blood supply ; transplantation ; Temporal Arteries