Adolescent ; Child ; Child, Preschool ; Electrocardiography ; Female ; Fever ; complications ; Hemorrhagic Fever with Renal Syndrome ; blood ; complications ; pathology ; Humans ; Hypergammaglobulinemia ; blood ; Immunoglobulin M ; blood ; Male ; Pain ; complications

The UHPLC-LTQ-Orbitrap high resolution mass spectrometer was used to explore the chemical compositions in safflower. The rapid separation of the compositions was conducted by the UHPLC, following by high resolution full scan and MS2 scan, under the positive and negative ion mode. The chemical formula of compositions were deduced by full scan data in less than 5, then the potential structures were confirmed by the MS2 data. Forty-nine compounds were detected, of which 26 was identified, and 5 compounds was validated by the standard substances.
Carthamus tinctorius ; chemistry ; Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Molecular Structure ; Tandem Mass Spectrometry ; methods

12 strains of H2-producing bacteria were isolated and purified from anaerobic sludge, aerobic sludge and river bottom sludge by Hungate method. A new species of high efficient hydrogen production bacterium Enterococcus sp. LG1 (registration number: EU258743 ) was studied deeply. It was showed that the Enterococcus sp. LG1 was an anaerobic and Gram-negative bacterium. Sequence analysis of this type of clones showed that it was affiliated with the genus Enterococcus and it was not reported yet in other paper at present. Meanwhile, batch tests of anaerobic fermentative hydrogen production by Enterococcus sp. LG1 were investigated by using sterilization pretreated sludge as substrate. The changes of soluble COD, protein, carbohydrate and pH value during hydrogen fermentation were monitored. It was found that only hydrogen and carbon dioxide were produced by this strain and no methane was detected during fermentation. The maximal hydrogen yield was 36.48 mL/g TCOD and the hydrogen concentration in the gas phase was 73.5%. The Enterococcus sp. LG1 was a butyrate fermentation bacteria analyzed by metabolites.

OBJECTIVETo observe the preventive protection of Kangdu Bufei Decoction (KBD) on acute severe respiratory syndrome (SARS) in medical personnel of Hong Kong at the epidemic period.

METHODSAt the epidemic period of SARS in Hong Kong, there were 2,601 medical staffs administered with KBD distributed by the Center. It was confirmed that 1,063 persons had taken it for successive two weeks according to the request and returned the effective questionnaire, they were regarded as the TCM group. The control group was consisted of 15,374 subjects who didn't take Chinese herbal medicine. Contents of the questionnaire including the condition of testee in terms of quality of life (QOL), changes of influenza-like symptoms and Warm disease symptoms. Serum immunological examination had been conducted in 37 of the persons in the TCM group.

RESULTSNo one in the TCM group got SARS infection while in the control group, 64 (0.4%) had got. Self-control before and after treatment examination showed that significant improvement appeared in the TCM in aspects of influenza-like and Warm disease symptoms and QOL. The results of serum immunological examination showed that after administration of KBD, the immunity of organism was improved and elevated.

CONCLUSIONKBD could prevent the occurrence of SARS, it is possibly realized through improving symptoms, elevating QOL and enhancing immunity of organism.

Adjuvants, Immunologic ; therapeutic use ; Adult ; Cross Infection ; prevention & control ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Hong Kong ; Humans ; Infectious Disease Transmission, Patient-to-Professional ; prevention & control ; Male ; Middle Aged ; Phytotherapy ; Severe Acute Respiratory Syndrome ; prevention & control ; transmission ; Surveys and Questionnaires

OBJECTIVETo study the adscription of plasma effective constituents of rat after oral administration of Paeonia lacliflora and Glycyrrhiza uralensis compound.

METHODBased on the established HPLC analytical method of plasma effective constituents, analysis and comparison were carried out among HPLC profiles of plasma samples obtained after oral administration of different ingredient preparations such as P. lacliflora and G. uralensis compound, single ingredient and all the preparations. The adscription of plasma effective compounds were identified.

RESULTEighteen compounds were detected under this method, three of which are metabolites and thirteen are original forum of compounds contained in P. laclflora and G. uralensis compound.

CONCLUSIONThis method is simple, accurate and stable, it could be used to analyze plasma effective constituents of P. lacliflora and G. uralensis compound. Affirmation of plasma effective compounds is the basis of to explore active compounds of P. lacliflora and G. uralensis compound.

Administration, Oral ; Animals ; Benzoates ; blood ; Bridged-Ring Compounds ; blood ; Chromatography, High Pressure Liquid ; Drug Combinations ; Drugs, Chinese Herbal ; administration & dosage ; isolation & purification ; pharmacokinetics ; Glucosides ; blood ; Glycyrrhetinic Acid ; blood ; Glycyrrhiza uralensis ; chemistry ; Glycyrrhizic Acid ; blood ; Male ; Monoterpenes ; Paeonia ; chemistry ; Plants, Medicinal ; chemistry ; Rats ; Rats, Sprague-Dawley

Objective To investigate the significance of the serum levels of interleukin-10 (IL-10),IL-13,IL-15 of patients with chronic hepatic failure and the correlation between those inter- leukin levels and nosocomial infections.Methods The serum levels of IL-10,IL-13,IL-15 of 58 patients with chronic hepatic failure were measured by double antibody sandwich enzyme-linked immu- nosorbent assay at the time of admission and 2 weeks after admission.Results The serum levels of IL-15 and the propotion of IL-15/IL-10 and IL-15/IL-13 in patients with chronic hepatic failure group at the time of admission were significantly higher than those in healthy control group[(358.16?290.91) ng/L vs (38.55?21.49) ng/L,12.93?14.26 vs 1.10?0.55,98.55?97.5.5 vs 9.70?5.03,respectively,all P=0.000].Those in death group were significantly higher than those in improving group[(479.93v205.52) ng/L vs (244.51?236.29) ng/L,17.65?17.78 vs 8.53?7.98,130.69?115.50 vs 68.55?65.99,respectively,all P

Objective To analyze the clinical speciality of invasive fungal infection(IFI)and provide doctors with clinical evidence for early anti-fungal therapy.Method One hundred and thirty-seven patients with 91 male and 46 female,who suffered from invasive fungal infection in ICU from January.1,2000 to June 30, 2006,were enrolled in this study.The age ranged from 17 to 82 years old.Out of 137 patients with IFI,the percentage of albicans candida,glabirate candida,tropicalis candida and parapsilosis candida were 47.4%, 26.3%,20.4% and 3.6%,reseparately.The sputum,urine,blood and other drainages were collected to perform the fungal examination after three days of admission every three days.Results Of 137 patients,42 of them were complicated with hemorrhage,53 patients with IFI developed candida anthema in the chest,abdomen and extremity.,49 patients suffering from IFI had organ dysfunction.The chest image revealed that infiltration caused by IFI especially occurred in apex of lung in some patients.The pathogen analysis displayed that albicans candidiasis easily developed candida anthema,glabirate candidiasis frequently resulted in organ dysfunction,and tropicalis candida led to hemorrhage in some organs.Conclusions The clinical specialty,of IFI caused by candida included hemorrhage,candida anthema,organ dysfunction,and infiltration in apex of lung.

OBJECTIVETo study the possibility of measuring quality of life by Medical Outcomes Study 36-Item Short-Form Health Survey scale and to subdivide grade range of Medical Outcomes Study 36-Item Short-From Health Survey (SF-36) total cents through a Quality of Life questionnaire among the elderly Chinese.

METHODSThe quality of life of the 167 elderly Chinese in Suzhou was measured simultaneously by SF-36 and the Quality of Life questionnaire developed by Epidemiology Group of Geriatric Medicine Committee of China for the elderly. Validity and reliability were analyzed and subdivided the grade range of SF-36 total scores by Quality of Life questionnaire for the Chinese elderly.

RESULTSEight common factors from factorial analysis were in accordance with their theoretical structure, and the cumulative contribution rates of the Quality of Life questionnaire for the elderly and SF-36 were 74.244% and 72.081%. The split-half reliability of the Quality of Life questionnaire for the elderly was 0.6676. The split-half reliability of SF-36 was 0.9384. In SF-36, the Cronbach's alpha coefficient of internal consistency reliability ranged from 0.81 to 0.89, which was satisfactory for group comparison except 0.63 for the social functioning and 0.42 for mental health scale and 0.69 for vitality scale. The Quality of Life questionnaire for old people seemed to have good validity and reliability but the SF-36 was better. The cent of the furthest truncation between the good quality of life and the medium one in the SF-36 was 117 with a Kappa value of 0.58.

CONCLUSIONThe SF-36 scale could be used for measuring and evaluating the quality of life for the Chinese elderly. The cent of the furthest truncation could provide reference to judge the level of the quality of life of the elderly.

Aged ; Aged, 80 and over ; China ; Female ; Humans ; Male ; Middle Aged ; Quality of Life ; Random Allocation ; Reproducibility of Results ; Surveys and Questionnaires ; standards

Objective To investigate the expressions of human telomerase reverse transcriptase(hTERT) mRNA and protein in pheochromocytoma and paraganglioma and their significance as diagnostic markers in predicting the biological behaviour of these tumours.Methods Expression of hTERT mRNA was determined by in situ hybridization in 45 pheochromocytomas/paragangliomas(31 benign,7 suspected malignant and 7 malignant) and 9 normal adrenal medulla samples,hTERT protein was determined by immunohistoebemistry.Results hTERT mRNA was expressed in 5/7 malignant turnouts and 5/7 suspected malignant tumours as compared with 3/31 benign tumours(P

OBJECTIVETo study the clinical feature of extracranial and intracranial complications caused by cholesteatoma otitis media.

METHODSA retrospective study as made of 78 patients with extracranial and intracranial complications caused by suppurative otitis media between January 1992 and December 2002 was conducted.

RESULTSFifty-six of the 78 patients had one complication and the other 22 patients had two or more complications among them. In this series, 24 cases had facial paralysis, 16 cases with suppurative labyrinthitis, 12 cases with meningitis, 11 cases with periauricular abscess, 9 cases with extradural abscess, 9 case with otogenic meningitis brain abscess, 6 case with lesion of sigmoid sinus and 5 cases with Bezold abscess. Of the 24 patients with facial paralysis, 15 with continued nerves had obtained omatomic preservation of the facial nerves, The nerve function of 11 patients recovered from House-Brackmann grade IV to grade II, the rest 4 patients didn't regain after operation. Four of the 9 patients with their facial nerves broken received anastomosis of the facial nerves, three cases received transplantation of the nervus auricularis magnus, and two received anastomosis to nervus auricularis magnus. The nerve function recovered from House-Brackmann grade V - IV to grade III.

CONCLUSIONSEven though the incidence of the complications has been low down in past years, there are still some serious complications. Temporal bone CT scan plays an important role in the diagnosis of extracranial and intracranial complications of cholesteatoma otitis media. The most reliable method to diagnosis and treat the complications is surgery.

Adolescent ; Adult ; Aged ; Brain Diseases ; etiology ; Child ; Child, Preschool ; Cholesteatoma, Middle Ear ; complications ; Facial Paralysis ; etiology ; Female ; Humans ; Intraoperative Complications ; Male ; Middle Aged ; Postoperative Complications ; Retrospective Studies ; Young Adult