3.Effect of human bone morphogenetic protein-2 on vascular endothelial growth factor expression in fetal mouse osteoblasts
De-Hao FU ; Shu-Hua YANG ; Bao-Jun XIAO ;
Chinese Journal of Orthopaedic Trauma 2004;0(08):-
Objective To investigate effects of different doses of recombinant human bone morphogenetic protein-2 (rhBMP-2) on vascular endothelial growth factor (VEGF) expression in fetal mouse osteoblasts.Methods Calvaria osteoblasts of fetal mice of 19 days were cultured.The effects of rhBMP-2 at different doses and different action times on VEGF expression patterns in fetal mouse osteoblasts were observed with reverse transcrip- tion-polymerase chain reaction (RT-PCR) and Western blot staining.Results In the present study,RT-PCR detected a steady expression of VEGF mRNA in the control fetal mouse osteoblasts.The levels of VEGF mRNA increased in an apparent biphasic manner with a maximum stimulation (about 2-fold above the control,P<0.05 ) in both VEGF mRNA species observed at 300 ng/mL of rbBMP-2.After 48 h of rhBMP-2 treatment,the VEGF mRNA levels approached those in the control.The VEGF mRNA levels appeared to be biphasic in rhBMP-2-treated cultures,showing peak induction at 3 and 24 h and remaining elevated at 48 b.Compared with the individual control value at each time point,an apparent maximum increase (about 2.5-fold above the control,P<0.05) occurred at 6 h.The second peak induction,about 2-fold above that in the control,occurred at about 36 h.Conclusions The expression of VEGF mRNA is steady in the control fetal mouse osteoblasts.RhBMP-2 can promote the expression of VEGF in dose-dependent and time-dependent manners.
5.Clinical observation of HRV in patients with coal worker's pneumoconiosis.
Ying BAO ; Li GUAN ; Xiu-yang LI ; De-jun WANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2005;23(2):154-155
Aged
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Coal Mining
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Electrocardiography
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Heart Rate
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physiology
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Humans
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Male
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Middle Aged
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Pneumoconiosis
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physiopathology
6.Evaluation of bubble oxygen inhalators' performances and an investigation on their solutions for improvement.
Mian-kang CHEN ; Zheng-hai SHEN ; Xun-liang XU ; Jun-cheng BAO ; Chang-shan ZUO ; De-jun TANG ; Jun YANG
Chinese Journal of Medical Instrumentation 2007;31(4):295-296
This paper analyses the defects of bubble oxygen inhalators currently used, and investigates into their solutions for improvement.
Oxygen Inhalation Therapy
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instrumentation
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methods
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Oxygenators
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standards
7.The application of psychometric measures in diagnosis of minimal hepatic encephalopathy
Zhi-Jun BAO ; De-Kai QIU ; Xiong MA ; Gansheng ZHANG ; Ting GU ; Xiaofeng YU ; Zhuping FAN ; Jiqiang LI ; Minde ZENG
Chinese Journal of Digestion 2001;0(09):-
Objective To establish the normal parameters of psychometric measures such as the number connection tests A(NCT-A)and digit symbol tests(DST)in assessment of minimal hepatic en- cephalopathy(MHE).Methods One hundred and sixty healthy volunteers(aged 25 to 64 years;educa- tional level>9 years)were divided into<35 ys,35~44 ys,45~54 ys and 55~64 ys groups.All of the healthy volunteers were assessed with NCT-A and DST to establish the normal value of age-related parameters,which can be used for diagnosis of MHE in patients with liver cirrhosis.Two standard devi- ation of the normal mean was used as a diagnostic criterion for MHE.One hundred and six cirrhotic patients were assessed with these parameters.Results The parameters of NCT-A were(25.1?4.6) sec in<35 ys group,(32.1?6.8) sec in 35~44 ys group,(38.6?7.1)sec in 45~54 ys group or (49.3?6.3)sec in 55~64 ys group.The scores of DST were 49.9?4.7 in<35 ys group,44.6?4.8 in 35~44 ys group,38.5?5.0 in 45~54 ys group or 35.4?4.7 in 55~64 ys group.Thirty one out of 106 cirrhotic patients were diagnosed as MHE based on these parameters.Conclusion The NCT- A and DST are psychometric assessments for diagnosis of MHE.Age-based normal paramerters of NCT- A and DST are needed to be established.
8.Effects of hyperbaric oxygen therapy performed at different times on cognitive dysfunction of rats with traumatic brain injury
Jun-De HE ; Ze-Yu XIE ; Bao CHEN ; Wen-Jun QU
Chinese Journal of Neuromedicine 2010;9(5):465-468
Objective To investigate the effects of hyperbaric oxygen (HBO) therapy performed at different times on the cognitive dysfunction of rats with traumatic brain injury. Methods The traumatic brain injured models were established by use of lateral fluid percussion in rats. Ninety-six rats were equally randomized into 4 groups: control group (group A), traumatic brain injured model group (group B), traumatic brain injured model plus conventional therapy group (group C), traumatic brain injured model plus both conventional therapy and HBO therapy group (group D). And group D also divided into 4 subgroups (n=6): D-3 h group, D-12 h group, D-24 h group and D-72 h groups that HBO therapy was performed at 3, 12, 24 and 72 h of the traumatic brain injury, respectively. The changes of learning and memory abilities before or after the therapy in each group were tested by Morris water maze method. Results Group B had the longest latency of finding the underwater platform as compared with the other 3 groups, followed by group C, group D and group A; as to the 4 sub-groups, D-72 h group had the longest latency of finding the underwater platform as compared with the other 3 groups, followed by group D-24 h, group D-12 h and group D-3 h. Group A could cross the original platform in the spatial probe test most often among all the 4 groups, followed by group D, group C and group B; group A, group C and group D was significantly different in clossing the original platform in the spatial probe test as compared with group B (P<0.05); group D-3 h could cross the original platform in the spatial probe test most often among all the 4 sub-groups, followed by group D-12 h, group D-24 h and group D-72 h.Conclusion HBO treatment can effectively improve the cognitive dysfunction of rats with traumatic brain injury, especially those within 12 h of injury.
10.A cadaveric study of relationships among rotational alignment reference axes of distal femur and tibial mechanical axis.
Bao-hui ZHAO ; Bai-cheng CHEN ; De-cheng SHAO ; Fei WANG ; Shi-jun GAO ; Bo LU
Chinese Journal of Surgery 2008;46(14):1085-1087
OBJECTIVESTo investigate the relationships among rotational alignment reference axes of distal femur and tibial mechanical axis, and determine the safest rotational alignment reference axis.
METHODSDigital photos were taken of 30 cadaveric lower extremities with knee in extension and flexion at 90 degrees , angles were measured among tibial mechanical axis and a line perpendicular to clinical epicondylar axis, a line perpendicular to surgical epicondylar axis, Whiteside's line and femoral mechanical axis. Statistical analysis of relationships among those axes were performed.
RESULTSThe angles among the tibial mechanical axis and a line perpendicular to the clinical epicondylar axis, a line perpendicular to the surgical epicondylar axis, Whiteside's line and femoral mechanical axis were 0.6 degrees varus, 3.9 degrees varus, 0.2 degrees valgus and 3.0 degrees varus respectively. The angle between the femoral mechanical axis and the tibial mechanical axis was significantly larger than the angles among the tibial mechanical axis and a line perpendicular to the clinical epicondylar axis, the Whiteside's line (P < 0.05). There was no significant difference compared with the angle between a line perpendicular to the surgical epicondylar axis and the tibial mechanical axis. Angles of the clinical epicondylar axis, the surgical epicondylar axis and the Whiteside's line between knee extension and flexion were 2.3 degrees valgus, 0.9 degrees varus and 3.1 degrees valgus respectively.
CONCLUSIONThe surgical epicondylar axis rather than the clinical epicondylar axis or the Whiteside's line is the safest femoral rotational alignment reference axis intraoperatively.
Arthroplasty, Replacement, Knee ; Biomechanical Phenomena ; Femur ; anatomy & histology ; surgery ; Humans ; Knee Prosthesis ; Rotation ; Tibia ; anatomy & histology ; surgery