Aged ; Anthracosis ; complications ; physiopathology ; Coronary Disease ; complications ; physiopathology ; Electrocardiography ; Humans ; Male ; Middle Aged ; Pulmonary Heart Disease ; complications ; physiopathology

Aged ; Arrhythmias, Cardiac ; diagnosis ; physiopathology ; Coal Mining ; Electrocardiography ; Humans ; Male ; Middle Aged ; Pneumoconiosis ; complications ; physiopathology

Graft vs Host Disease ; metabolism ; surgery ; Graft vs Tumor Effect ; HLA Antigens ; genetics ; Hematopoietic Stem Cell Transplantation ; Humans ; Receptors, KIR ; genetics ; metabolism

Aged ; Coal Mining ; Electrocardiography ; Heart Rate ; physiology ; Humans ; Male ; Middle Aged ; Pneumoconiosis ; physiopathology

Objective To investigate effects of different doses of recombinant human bone morphogenetic protein-2 (rhBMP-2) on vascular endothelial growth factor (VEGF) expression in fetal mouse osteoblasts.Methods Calvaria osteoblasts of fetal mice of 19 days were cultured.The effects of rhBMP-2 at different doses and different action times on VEGF expression patterns in fetal mouse osteoblasts were observed with reverse transcrip- tion-polymerase chain reaction (RT-PCR) and Western blot staining.Results In the present study,RT-PCR detected a steady expression of VEGF mRNA in the control fetal mouse osteoblasts.The levels of VEGF mRNA increased in an apparent biphasic manner with a maximum stimulation (about 2-fold above the control,P＜0.05 ) in both VEGF mRNA species observed at 300 ng/mL of rbBMP-2.After 48 h of rhBMP-2 treatment,the VEGF mRNA levels approached those in the control.The VEGF mRNA levels appeared to be biphasic in rhBMP-2-treated cultures,showing peak induction at 3 and 24 h and remaining elevated at 48 b.Compared with the individual control value at each time point,an apparent maximum increase (about 2.5-fold above the control,P＜0.05) occurred at 6 h.The second peak induction,about 2-fold above that in the control,occurred at about 36 h.Conclusions The expression of VEGF mRNA is steady in the control fetal mouse osteoblasts.RhBMP-2 can promote the expression of VEGF in dose-dependent and time-dependent manners.

This paper analyses the defects of bubble oxygen inhalators currently used, and investigates into their solutions for improvement.
Oxygen Inhalation Therapy ; instrumentation ; methods ; Oxygenators ; standards

Objective To establish the normal parameters of psychometric measures such as the number connection tests A(NCT-A)and digit symbol tests(DST)in assessment of minimal hepatic en- cephalopathy(MHE).Methods One hundred and sixty healthy volunteers(aged 25 to 64 years;educa- tional level＞9 years)were divided into＜35 ys,35～44 ys,45～54 ys and 55～64 ys groups.All of the healthy volunteers were assessed with NCT-A and DST to establish the normal value of age-related parameters,which can be used for diagnosis of MHE in patients with liver cirrhosis.Two standard devi- ation of the normal mean was used as a diagnostic criterion for MHE.One hundred and six cirrhotic patients were assessed with these parameters.Results The parameters of NCT-A were(25.1?4.6) sec in＜35 ys group,(32.1?6.8) sec in 35～44 ys group,(38.6?7.1)sec in 45～54 ys group or (49.3?6.3)sec in 55～64 ys group.The scores of DST were 49.9?4.7 in＜35 ys group,44.6?4.8 in 35～44 ys group,38.5?5.0 in 45～54 ys group or 35.4?4.7 in 55～64 ys group.Thirty one out of 106 cirrhotic patients were diagnosed as MHE based on these parameters.Conclusion The NCT- A and DST are psychometric assessments for diagnosis of MHE.Age-based normal paramerters of NCT- A and DST are needed to be established.

Objective To investigate the effects of hyperbaric oxygen (HBO) therapy performed at different times on the cognitive dysfunction of rats with traumatic brain injury. Methods The traumatic brain injured models were established by use of lateral fluid percussion in rats. Ninety-six rats were equally randomized into 4 groups: control group (group A), traumatic brain injured model group (group B), traumatic brain injured model plus conventional therapy group (group C), traumatic brain injured model plus both conventional therapy and HBO therapy group (group D). And group D also divided into 4 subgroups (n=6): D-3 h group, D-12 h group, D-24 h group and D-72 h groups that HBO therapy was performed at 3, 12, 24 and 72 h of the traumatic brain injury, respectively. The changes of learning and memory abilities before or after the therapy in each group were tested by Morris water maze method. Results Group B had the longest latency of finding the underwater platform as compared with the other 3 groups, followed by group C, group D and group A; as to the 4 sub-groups, D-72 h group had the longest latency of finding the underwater platform as compared with the other 3 groups, followed by group D-24 h, group D-12 h and group D-3 h. Group A could cross the original platform in the spatial probe test most often among all the 4 groups, followed by group D, group C and group B; group A, group C and group D was significantly different in clossing the original platform in the spatial probe test as compared with group B (P<0.05); group D-3 h could cross the original platform in the spatial probe test most often among all the 4 sub-groups, followed by group D-12 h, group D-24 h and group D-72 h.Conclusion HBO treatment can effectively improve the cognitive dysfunction of rats with traumatic brain injury, especially those within 12 h of injury.

OBJECTIVETo study effects of the modified sandblasted surface of titanium implants, developed by authors, on the bone healing process.

METHODSOsteoblasts were derived from the 5th passage of human fetal osteoblasts after primary isolation. Alkaline phosphatase (ALP) activities and protein contents of cellular layers, and osteocalcin contents in culturing medium were employed as criteria to evaluate osteogenic functions and differentiation of osteoblasts. The ALP activity was assayed utilizing the kinetic method, the protein content utilizing the Coomassie's method, and the osteocalcin content utilizing the radioimmune assay (RIA) method. Values of all criteria were divided by the corresponding cell numbers of different groups at a respective time point for the purpose of standardization. Samples were assigned to three groups-the modified sandblasted surface group, the smooth surface group and the blank control group, The culture was ended at, 4 days and 13 days.

RESULTSAt 4 days of culture, the modified sandblasted surface group showed a superiority to the smooth group with respects to the ALP activity [(17.390 +/- 1.595) nmol PNP x min(-1) x 10(-6) cells vs. (10.978 +/- 1.879) nmol PNP x min(-1) x 10(-6) cells] and protein content [(152.7 +/- 16.3) micro g/10(6) cells vs. (58.0 +/- 5.9) micro g/10(6) cells] of cellular layers and the osteocalcincontent [(43.0 +/- 6.1) ng/10(6) cells vs. (24.9 +/- 6.0) ng/10(6) cells] in culturing medium. Till the 13th day of culture, no differences were detected.

CONCLUSIONSIt is cytologically proved that the modified sandblasted surface can accelerate the bone healing process of implants though the improvement of osteoblastic functions and differentiation.

OBJECTIVETo investigate the research on neonatal jaundice in recent years by co-word analysis and to summarize the hot spots and trend of research in this field in China.

METHODSThe CNKI was searched with "neonate" and "jaundice" as the key words to identify the papers published from January 2009 to July 2013 that were in accordance with strict inclusion and exclusion criteria. To reveal the relationship between different high-frequency key words, Microsoft Office Excel 2013 was used for statistical analysis of key words, and Ucinet 6.0 and Netdraw were used for co-occurrence analysis.

RESULTSA total of 2 054 papers were included, and 44 high-frequency key words were extracted. The current hotspots of research on neonatal jaundice in China were displayed, and the relationship between different high-frequency key words was presented.

CONCLUSIONSThere has been in-depth research on clinical manifestations and diagnosis of neonatal jaundice in China, but further research is needed to investigate the etiology, mechanism, and treatment of neonatal jaundice.