1.Changes of gene expression profile in homoharringtonine-induced leukemia multi-drug resistant cell line K562/HHT.
Xiao-Lin YU ; Yong LIU ; Juan-Dong WANG ; De-Xiao KONG ; Chun-Yan CHEN
Chinese Journal of Hematology 2009;30(6):363-367
OBJECTIVETo study the resistant related molecules of human leukemia drug resistant K562 cells (K562/HHT) induced by homoharringtonine (HHT).
METHODSGene expression profiles on K562/HHT, K562 and K562/HHT/RU486 (K562/HHT reversed by RU486) cells were detected by DNA microarray. The bone marrow tyrosine kinase gene in chromosome X (BMX) which changed dynamically among the three cells was confirmed by RT-PCR and Western blot. Then, BMX was transfected into K562 and K562/HHT cells, and the changes of daunorubicin (DNR) concentrations in these two cells were observed for BMX overexpression.
RESULTSAs compared with K562, there were changes in 117 gene expressions in K562/HHT, 57 of which were up-regulated and 60 down-regulated. The mdrl gene was significantly up-regulated. When compared with K562/HHT, 50 significantly differently expressed genes were screened out in the K562/HHT/RU486 cells, of which up- and down-regulated genes were 13 and 37 respectively. These genes involved in drug resistance, cell signaling, cell differentiation, cell proliferation, transcription regulator, ion transport and so on. Four genes [NM-001721 (BMX), NM-031459 (SESN2), NM-033642 (FGF13) and AL-049309 (SFRS12)] expressed significantly differently in the two group cells, BMX gene expression was higher in K562/HHT, than in K562, but lower than in K562/HHT/RU486 as confirmed by RT-PCR and Western blot. After the plasmid pCI-neo-BMX was transfected into K562 and K562/HHT cells, DNR concentration was significantly lower (79.28 +/- 4.04, 29.84 +/- 2.67) than those before transfection (158.52 +/- 8.08, 58.58 +/- 6.53).
CONCLUSIONBMX is associated with multi-drug resistance of K562/HHT cell line.
Drug Resistance, Multiple ; drug effects ; genetics ; Drug Resistance, Neoplasm ; drug effects ; genetics ; Gene Expression Profiling ; Harringtonines ; pharmacology ; Humans ; K562 Cells ; Leukemia ; drug therapy ; genetics ; metabolism ; Protein-Tyrosine Kinases ; genetics ; metabolism
2.Effect of tonifying shen recipe on advanced glycosylation end products in aorta and serum lipids in ovariectomized rats.
Xi-zhen GE ; De-juan KONG ; En LI
Chinese Journal of Integrated Traditional and Western Medicine 2003;23(4):288-290
OBJECTIVETo investigate the effect of tonifying Shen recipe (TSR) on levels of advanced glycosylation end products (AGEs) in aorta, serum lipids and lipid peroxidation in ovariectomized rats.
METHODSRats were randomly divided into the sham group, the ovariectomized group and the TSR group, in which the rats were treated with TSR for 13 weeks starting from 2 weeks after ovariectomy. Blood sample was taken out from rat at the end of the experiment after 24 hrs fasting for determination of lipids and lipid peroxidation, and the animal was sacrificed, the aorta was taken out for detecting AGEs.
RESULTSNo significant difference was found between groups in levels of total cholesterol and low density lipoprotein cholesterol. In comparing with the sham group, levels of aortic AGEs, serum triglyceride (TG), oxidized low density lipoprotein (OX-LDL) and malondialdehyde (MDA) in the ovariectomized group were obviously higher (P < 0.05 or P < 0.01), and levels of high density lipoprotein cholesterol (HDL-C), apo-lipoprotein A-I (apoA-I) and activity of superoxide dismutase (SOD) were lower (all P < 0.01). While in the TSR group, as compared with the ovariectomized group, the above-mentioned abnormal changes, excepting for TG, were all reversed to certain degree (P < 0.05 or P < 0.01).
CONCLUSIONTSR displays its cardiovascular protecting effect in ovariectomized rats through lowering the AGEs content in aorta, reducing the serum levels of OX-LDL and MDA, raising the levels of serum HDL-C and apoA-I and increasing SOD activity.
Animals ; Aorta ; metabolism ; Female ; Glycation End Products, Advanced ; metabolism ; Lipid Peroxidation ; drug effects ; Lipids ; blood ; Ovariectomy ; Random Allocation ; Rats ; Rats, Sprague-Dawley
3.Social support and its influencing factors of postoperative cancer patients undergoing chemotherapy
Jiag ZHANG ; Zheng LI ; De-Juan KONG
Chinese Journal of Modern Nursing 2008;14(15):1659-1661
Objective To explore the state of social support and its influencing factors in patients with cancer diagnosis during chemotherapy in China. Methods 294 cancer subjects received first chemotherapy after operation were investigated with questionare, including social support scale and basic demographic form. Results The average score of social support was (46.26±6.96) , for objective social support was (12.05±3.18), subjective social support was (26.57±4.07) and utilization of social support was (7.63±2.23). Income and marital status influenced the state of social support(F=30. 43, F=18.46, P<0. 001). Conclusions The results show that the most cancer patients during chemotherapy have higher level of social support, Identified affecting factors of the state of social support were income and marital status. Therefore, nurses should help patients to use social support to improve their mental health.
4.In vitro activation of bone marrow natural killer T cells of aplastic anemia patients.
Ying-Xue WANG ; Cong-Gao XU ; Jun-Li RAN ; Xin-Chun WU ; Jun-Hua SUN ; Juan-Dong WANG ; Cheng-Shan GUO ; Jun-Li LIU ; De-Xiao KONG ; Ai-Xia DOU
Chinese Journal of Hematology 2010;31(8):536-539
OBJECTIVETo investigate the quantitative and qualitative changes of TCRVα24(+)Vβ11(+) natural killer T (NKT) cells from bone marrow (BM) of aplastic anemia (AA) after in vitro stimulation of α-galactosylceramide (α-Galcer).
METHODSNKT cells in the bone marrow mononuclear cells (BMMNCs) from either AA patients or healthy controls were enumerated with flow cytometry. BMMNCs were cultured in RPMI1640 medium supplemented with either α-Galcer and rhIL-2 or α-Galcer, rhIL-2 and rhG-CSF. The proliferative capacity of NKT cells was determined by NKT cell numbers before and after in vitro culture. Expression of intracellular IFNγ and IL-4 in activated NKT cells was analyzed with flow cytometry.
RESULTSIn AA group, the percentage of NKT cells in BMMNCs was (0.19 ± 0.09)%. Addition of rhG-CSF into the α-Galcer/rhIL-2 culture medium resulted in significantly reduced expansion of NKT cells (67.45 ± 29.42-fold vs 79.91 ± 40.56 fold, P < 0.05). Meanwhile, addition of rhG-CSF reduced IFNγ positive NKT cells \[(37.45 ± 7.89)% vs (62.31 ± 14.67)%, P < 0.01\] and increased IL-4 positive NKT cells \[(55.11 ± 12.13)% vs (27.03 ± 9.88)%, P < 0.01\]. In healthy control group, the percentage of NKT cells in BMMNCs was (0.25 ± 0.12)%. Addition of rhG-CSF into the α-Galcer/rhIL-2 culture medium also significantly reduced expansion of NKT cells (97.91 ± 53.22-fold vs 119.58 ± 60.49-fold, P < 0.05), reduced IFNγ positive NKT cells \[(28.65 ± 10.63)% vs (50.87 ± 12.66)%, P < 0.01\], and increased IL-4 positive NKT cells \[(66.53 ± 14.96)% vs (31.11 ± 10.07)%, P < 0.01\].
CONCLUSIONCompared to those from healthy controls, BMMNCs from AA patiants have a reduced fraction of NKT cells, which possesses a decreased potential to expand in vitro in response to α-Galcer stimulation, and produce more IFNγ(+) NKT1 cells. rhG-CSF, in combination with α-Galcer, confers polarization of NKT cells towards IL-4(+) NKT2 subpopulation.
Anemia, Aplastic ; metabolism ; Bone Marrow ; metabolism ; Humans ; Interleukin-4 ; metabolism ; Killer Cells, Natural ; cytology ; Natural Killer T-Cells
5.Etiology analysis for hospitalized hypertensive patients: 10 years report from the department of hypertension (1999-2008)
Nan-Fang LI ; Li LIN ; Lei WANG ; Xin-Ling WANG ; Fei-Ya ZU ; De-Lian ZHANG ; Gui-Juan CHANG ; Yan-Nin ZHANG ; Ke-Ming ZHOU ; Nuerguli ; Tao LI ; Jun-Li HU ; Jian-Qiong KONG ; Men-Hui WANG ; Qin LUO
Chinese Journal of Cardiology 2010;38(10):939-942
Objective To analyze etiology of hospitalized hypertensive patients in the department of hypertension from 1999 to 2008. Methods This retrospective study was performed to analyze the etiology of hospitalized hypertensive patients in department of hypertension and to show the distribution change of hypertension from 1999 to 2008. Results ( 1 ) There were 5867 ( 75. 1% ) patients with essential hypertension and 1942 (24. 9% ) patients with secondary hypertension (SH). (2) The prevalence rate of SH increased significantly during the 10 years (χ2 = 387.621 ,P < 0.001 ) and was higher in 2008 than in 1999 (39. 3% vs. 9. 5% , P < 0. 05 ). The prevalence of obstructive sleep apnea syndrome (OSAS) and primary aldosteronism (PA) in 2008 increased 38.3 and 1.8 times respectively than in 1999 (χ2 =304. 025 ,P <0. 001; χ2 =42. 845 ,P <0. 001 ) and other SH remained unchanged. ( 3 ) The prevalence of PA complicated with OSAS increased significantly in recent five years ( χ2 = 26.376, P <0. 001 ). Incidence of OSAS was 23.9% in PA patients and incidence of PA was 6. 7% in OSAS patients. Conclusions With the insights gained on hypertension mechanism and the development of new diagnostic technology, percent of diagnosed SH increased remarkably in recent years in hospitalized hypertensive patients in our department of hypertension. OSAS and PA are the leading causes of SH.
6.A Suspected Person-to-person Transmission of Avian Influenza A (H7N9) Case in Ward.
Ze-Hua ZHANG ; Ling-Sheng MENG ; De-Hua KONG ; Jun LIU ; Si-Zhao LI ; Chao ZHOU ; Juan SUN ; Ren-Jie SONG ; Jin-Ju WU
Chinese Medical Journal 2017;130(10):1255-1256
7.Analysis of the of Pb, Cd and As in decoction of Lindera aggregata (Sims) Kosterm. by PBET digestion in vitro /Caco-2 cell model and their cumulative risk assessment
Tian-tian ZUO ; Ya-qiong SUO ; Fei-ya LUO ; De-juan KONG ; Hong-yu JIN ; Lei SUN ; Shu-xia XING ; Yuan-sheng GUO ; Gang-li WANG ; Shuang-cheng MA
Acta Pharmaceutica Sinica 2023;58(8):2461-2467
Inductively coupled plasma mass spectrometry (ICP-MS) was applied to determine the concentrations of lead (Pb), cadmium (Cd) and arsenic (As) in
8.Prohibitin (PHB) interacts with AKT in mitochondria to coordinately modulate sperm motility.
Xiao-Hui LI ; Ran-Ran CHAI ; Guo-Wu CHEN ; Ling-Fei ZHANG ; Wen-Jing TAN-TAI ; Hui-Juan SHI ; Patricia A MARTIN-DELEON ; Wai-Sum O ; Hong CHEN
Asian Journal of Andrology 2020;22(6):583-589
Prohibitin (PHB), an evolutionarily conserved mitochondrial inner membrane protein, is highly expressed in cells that require strong mitochondrial function. Recently, we demonstrated that the deletion of Phb in spermatocytes results in impaired mitochondrial function. In addition, PHB expression in the mitochondrial sheath of human sperm has a significantly negative correlation with mitochondrial reactive oxygen species levels, but a positive one with mitochondrial membrane potential and sperm motility. These results suggest that mitochondrial PHB expression plays a role in sperm motility. However, the mechanism of PHB-mediated regulation of sperm motility remains unknown. Here, we demonstrate for the first time that PHB interacts with protein kinase B (AKT) and exists in a complex with phospho-PHB (pT258) and phospho-AKT in the mitochondrial sheath of murine sperm, as determined using colocalization and coimmunoprecipitation assays. After blocking AKT activity using wortmannin (a phosphatidylinositol 3-kinase [PI3K] inhibitor), murine sperm have significantly ( P < 0.05) decreased levels of phospho-PHB (pT258) and the total and progressive motility. Furthermore, significantly ( P < 0.05) lower levels of phospho-PI3K P85 subunit α+γ (pY199 and pY467) and phospho-AKT (pS473; pT308) are found in sperm from infertile asthenospermic and oligoasthenospermic men compared with normospermic subjects, which suggest a reduced activity of the PI3K/AKT pathway in these infertile subjects. Importantly, these sperm from infertile subjects also have a significantly ( P < 0.05) lower level of phospho-PHB (pT258). Collectively, our findings suggest that the interaction of PHB with AKT in the mitochondrial sheath is critical for sperm motility, where PHB phosphorylation (pT258) level and PI3K/AKT activity are key regulatory factors.
9.Study on the mechanism of Wuzi-Yanzong-Wan-medicated serum interfering with the mitochondrial permeability transition pore in the GC-2 cell induced by atractyloside.
De-Ling WU ; Tong-Sheng WANG ; Hong-Juan LIU ; Wei ZHANG ; Xiao-Hui TONG ; Dai-Yin PENG ; Ling-Yi KONG
Chinese Journal of Natural Medicines (English Ed.) 2022;20(4):282-289
Wuzi-Yanzong-Wan (WZYZW) is a classic prescription for male infertility. Our previous investigation has demonstrated that it can inhibit sperm apoptosis via affecting mitochondria, but the underlying mechanisms are unclear. The purpose of the present study was to explore the actions of WZYZW on mitochondrial permeability transition pore (mPTP) in mouse spermatocyte cell line (GC-2 cells) opened by atractyloside (ATR). At first, WZYZW-medicated serum was prepared from rats following oral administration of WZYZW for 7 days. GC-2 cells were divided into control group, model group, positive group, as well as 5%, 10%, 15% WZYZW-medicated serum group. Cyclosporine A (CsA) was used as a positive control. 50 μmol·L-1 ATR was added after drugs incubation. Cell viability was assessed using CCK-8. Apoptosis was detected using flow cytometry and TUNEL method. The opening of mPTP and mitochondrial membrane potential (MMP) were detected by Calcein AM and JC-1 fluorescent probe respectively. The mRNA and protein levels of voltage-dependent anion channel 1 (VDAC1), cyclophilin D (CypD), adenine nucleotide translocator (ANT), cytochrome C (Cyt C), caspase 3, 9 were detected by RT-PCR (real time quantity PCR) and Western blotting respectively. The results demonstrated that mPTP of GC-2 cells was opened after 24 hours of ATR treatment, resulting in decreased MMP and increased apoptosis. Pre-protection with WZYZ-medicated serum and CsA inhibited the opening of mPTP of GC-2 cells induced by ATR associated with increased MMP and decreased apoptosis. Moreover, the results of RT-qPCR and WB suggested that WZYZW-medicated serum could significantly reduce the mRNA and protein levels of VDAC1 and CypD, Caspase-3, 9 and CytC, as well as a increased ratio of Bcl/Bax. However, ANT was not significantly affected. Therefore, these findings indicated that WZYZW inhibited mitochondrial mediated apoptosis by attenuating the opening of mPTP in GC-2 cells. WZYZW-medicated serum inhibited the expressions of VDAC1 and CypD and increased the expression of Bcl-2, which affected the opening of mPTP and exerted protective and anti-apoptotic effects on GC-2 cell induced by ATR.
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
;
Animals
;
Atractyloside/pharmacology*
;
Cyclophilin D
;
Male
;
Matrix Metalloproteinases
;
Mice
;
Mitochondrial Membrane Transport Proteins/metabolism*
;
Mitochondrial Permeability Transition Pore
;
RNA, Messenger
;
Rats
10.Recovery in schizophrenia: Perspectives from psychiatrists in the Philippines.
Michael P. Sionzon ; Chelseah Denise H. Torres ; Carl Abelardo T. Antonio ; Carlo Eugenio V. Banaag ; Ma. Lourdes Rosanna E. de Guzman ; Monina Garduñ ; o-Cruz ; Mariano S. Hembra ; Benita Sta. Ana-Ponio ; Edgardo Juan L. Tolentino, Jr. ; Jessa Joy C. Malipot ; Dorothy Jean N. Ortega ; Ma. Sophia Graciela L. Reyes ; Angelica Beth T. Sia ; Charlene J. Tinaja ; Patrick Joseph Aldea ; Erwin G. Benedicto
Journal of the Philippine Medical Association 2019;97(2):52-60
BACKGROUND:
A reliable and socially validated definition of recovery in schizophrenia is essential to decrease
stigma associated with the illness. This study aimed to define recovery in schizophrenia in the Philippine context,
determine its specific elements, and describe methods of assessment in clinical practice.
METHODS:
We invited a group of purposively selected Filipino psychiatrists to participate in six simultaneous
roundtable discussions to gather their opinions and perspectives on recovery in schizophrenia. Transcripts of the
discussions were then subjected to framework analysis.
RESULTS AND CONCLUSION
Most Filipino psychiatrists were of the considered opinion that recovery in schizophrenia
is possible, and their vision of a recovered patient resembles a combination of psychological and medical models.
The mini-FROGS tool was deemed generally applicable in the Philippine setting except for self-esteem and sense of
independence primarily because it is difficult to evaluate. The SWN was received with mixed reactions among the
psychiatrists. Spirituality as an element of recovery and the family-oriented culture of the Filipinos were emphasized as important considerations in assessing patients. Other suggestions were given to tailor-fit these tools to
the Philippine context.
Schizophrenia
;
Philippines