Objective: To investigate the effect of different degrees of ischemic white matter lesions on the cognitive function in patients with lacunar infarction. Method: One hundred twelve consecutive patients with lacunar infarction were collected. Age-related white matter changes rating scale (ARWMCRs) was used, and according to the results of ARWMCRs scores, the patients were divided into mild (n = 34, 0-3 scores), moderate (n = 43, 4-7 scores), and severe (n = 35, 8-24 scores) groups. The national health institutes of stroke scale (NIHSS) and the modified Rankin scale (mRS) scores were used to assess the degree of neurological deficit. The mini-mental status scale (MMSE) and the Montreal Cognitive Assessment (MoCA) were used for neuropsychological tests, and event-related potential two-tone sequence auditory P300 (2t-P300) wave was used for neural electrophysiological examination. The cognitive function was evaluated. The cognitive function among all groups was compared. Results: Circled digit oneThere were significant differences in comparison of the MoCA scores among the three groups. Only the mRS score and the MMSE score in the severe group were lower than those in the mild and moderate groups. There were significant differences (P < 0.05). Circled digit twoThe latency of P300 wave in the severe group was higher than that in the moderate and mild groups, and the latency of P300 wave in the moderate group was higher than that in the mild group. There was significant difference (P <0.05). The amplitude of P300 wave in the severe group was lower than that in the moderate group and mild group. There were significant differences (P < 0.05). Circled digit threeThe ARWMCRs score in patients with white matter lesions was negatively correlated with the MMSE score, MoCA score, and amplitude of P300 wave. It was positively correlated with the latency of P300 wave and mRS score. Conclusion: White matter lesions impact the cognitive function in patients with lacunar infarction. The more severe the white matter lesions, the more significant decline in cognitive function.

Mathematics ; Models, Theoretical ; Occupational Health Services ; organization & administration ; Risk Assessment ; methods

OBJECTIVETo bring forward a method to improve the prominence of the forehead contour.

METHODS16 patients(age 29-53 years, female 11, male 5) were treated through a bicoronal incision. Following the incision, the forehead soft tissues are reflected down to the level of the superior orbital rims with the protection to the supraorbital neurovascular bundles. To adjust the forehead contour, both superior orbital rims and frontal bossing was reduced by burring down the excess bone or removing the anterior wall of the forehead.

RESULTSThe operative effect were satisfactory during 3-12 months of follow-up. No recurrence or osteogenesis were found in the cases. Nasofrontal angle and forehead angle were normal. The prominence of the supraorbital ridges disappeared and forehead became nature.

CONCLUSIONCranioplasty via an osteomy or burring of the outer line of forehead bone are safe, compact and effective way to improve the prominence of the superior orbital rims or frontal bossing.

Adult ; Female ; Forehead ; surgery ; Humans ; Male ; Middle Aged ; Reconstructive Surgical Procedures ; methods

BACKGROUNDPeripheral T-cell lymphoma (PTCL) is generally characterized by poor prognosis after conventional chemotherapy. The place for high-dose chemotherapy and autologous stem cell transplantation (ASCT) in these patients is still not clear. In this study, we presented the outcomes of PTCL patients followed these treatments in our centre.

METHODSWe retrospectively analyzed the outcomes of 39 patients with PTCL received the two treatments between 1999 and 2010.

RESULTSThe 3-year overall survival (OS) of 61.9% and 3-year progression free survival (PFS) of 35.7% were observed in the 39 patient. Twenty-one patients received Hyper-CVAD chemotherapy with 3-year OS of 46.2% and 3-year PFS of 27.9%. Eighteen patients received ASCT with 3-year OS of 70.3% and 3-year PFS of 44.2%. Further analysis revealed that patients with elevated lactate dehydrogenase, at least 2 international prognostic index (IPI) points, and extranodal involvement had a poorer outcome compared with the control group.

CONCLUSIONThese findings might suggest that Hyper-CVAD chemotherapy and ASCT could offer a durable survival benefit for patients with aggressive PTCL.

Adolescent ; Adult ; Aged ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Cyclophosphamide ; therapeutic use ; Dexamethasone ; therapeutic use ; Doxorubicin ; therapeutic use ; Female ; Hematopoietic Stem Cell Transplantation ; methods ; Humans ; Lymphoma, T-Cell, Peripheral ; drug therapy ; therapy ; Male ; Middle Aged ; Retrospective Studies ; Vincristine ; therapeutic use ; Young Adult

We studied on the bio-safety problem of cultivating innovative talents in medical microbiology. The bio-safety of laboratory was controlled by educating bio-safety before the experiments, regulating basic operations during the experiments and constructing management system out of the experiments. Then we got some experience to ensure students’ bio-safety during the research.

No abstract available.
DNA* ; Heart* ; Humans*

OBJECTIVETo observe the changes in systemic hemodynamics and their relations to the concentrations of nitric oxide, endothelin, prostacyclin, and thromboxane A2 after portal cavity clamping and opening in portal hypertensive canines.

METHODSTwelve canines were randomly divided into control group and model group, and partial ligation of the portal vein was performed in the model group. Portal cavity clamping and opening was performed 12 weeks later in the two groups. The hemodynamic parameters including cardiac output index (CI), heart rate (HR), mean artery blood pressure (MABP), central venous pressure (CVP), pulmonary arteriole wedge pressure (PAWP), and systemic vascular resistance index (SVRI) were measured during the operation. Samples were obtained from the central vein at 3 time points during the operation for measuring NO, ET, PGI2, and TXA2.

RESULTSPortal vein ligation and portal cavity clamping produced obvious changes in the systemic circulation of the dogs, and the alteration was milder in the control group. After obstruction of the portal vein, the NO levels in systemic circulation in portal hypertensive dogs declined obviously, but gradually recovered the normal level after reperfusion.

CONCLUSIONSystemic circulation undergoes significant alterations after portal vein obstruction, but its changes in portal hypertensive dogs are milder than those in the control group, the mechanism of which needs further investigation.

Animals ; Disease Models, Animal ; Dogs ; Endothelins ; blood ; Epoprostenol ; blood ; Hemodynamics ; Hypertension, Portal ; blood ; physiopathology ; Nitric Oxide ; blood ; Plasma ; metabolism ; Portal Vein ; physiopathology ; Thromboxane A2 ; blood ; Vena Cava, Inferior ; physiopathology

AIMTo study the effects of hydrocortisone sodium succinate on sodium current in human atrial myocytes and in guinea pig ventricular myocytes.

METHODSSingle cardiac myocytes were isolated by enzyme. The effects of hydrocortisone sodium succinate on sodium current (INa) were assessed by applying whole-cell patch clamp techniques.

RESULTSHydrocortisone sodium succinate (1, 3, 10 micromol x L(-1)) was shown to inhibit INa of both human atrial myocytes and guinea pig ventricular myocytes in concentration dependent manner and the IC50 were 6.97 and 8.74 micromol x L(-1), respectively. The inhibition effects acted quickly (1-3 min) and the maximal activating voltage of INa was not changed in both human and guinea pig cardiac myocytes.

CONCLUSIONHydrocortisone sodium succinate can exhibit inhibitory effects on INa in both human and guinea pig cardiac myocytes, and its inhibitory effects act rapidly, which are not consistent with genomic effects, so there may be nongenomic effects.

Adolescent ; Adult ; Animals ; Cell Separation ; Child ; Child, Preschool ; Guinea Pigs ; Heart Atria ; pathology ; Heart Defects, Congenital ; pathology ; Heart Ventricles ; cytology ; Humans ; Hydrocortisone ; analogs & derivatives ; pharmacology ; Myocytes, Cardiac ; drug effects ; physiology ; Patch-Clamp Techniques ; Sodium Channels ; drug effects

OBJECTIVETo evaluate the influence of borneol on nasal absorption enhancement of ligustrazine.

METHODThe rats were randomly divided into 3 groups: Xiongbing nasal spray group (group A), Chuanxiong nasal spray group (group B) and Xiongbing group by decoction intragastric administration (group C). All the rats were decapitated at several time points after administration. The whole brains of rats were taken to homogenate and detected the concentrations of ligustrazine.

RESULTCompared with group B and C, group A has its characteristics: quick absorption, quick distribution and quick excretion of ligustrazine.

CONCLUSIONNasal administration is a quick-acting way for ischemic cerebral vessel insufficientia. Borneol promoted nasal absorption of ligustrazine into brain.

Animals ; Biological Transport ; drug effects ; Bornanes ; pharmacology ; Drug Administration Routes ; Female ; Kinetics ; Male ; Nose ; drug effects ; metabolism ; Pyrazines ; administration & dosage ; pharmacokinetics ; Rats ; Rats, Sprague-Dawley

OBJECTIVETo improve the outcome of the face-lift with a craniofacial contouring procedure.

METHODSThirty-seven patients aged 29-53 years (31 in female, 6 in male) were treated through a bicoronal incision. The facial tissue was lifted through a subperiosteal procedure and the facial skeleton was remodeled in three dimensions by osteotomies.

RESULTSThe results were satisfactory after the 3-12 months of the follow-ups.

CONCLUSIONSThe Face-lift combined with the facial aesthetic sculpturing may be more effective for the aging-face rejuvenating.

Adult ; Esthetics ; Female ; Humans ; Male ; Middle Aged ; Rhytidoplasty ; methods