Abstract: Objective　To investigate the clinical and laboratory characteristics of pulmonary infection caused by Nocardia otitidiscaviarum. Methods　The clinical data of a patient with pulmonary infection caused by Nocardia otitidiscaviarum were reported, and the clinical characteristics, laboratory characteristics and drug sensitivity of pulmonary infection caused by Nocardia otitidiscaviarum were summarized in combination with the relevant literature at home and abroad from January 2010 to December 2022. Results　A 67-year-old female patient was admitted to the hospital on June 30, 2020 because of "repeated chest tightness and shortness of breath for 3 years, aggravated cough, expectoration and fever". The sputum, alveolar lavage fluid and blood of the patient were collected for culture, and the detected pathogenic bacteria were identified. There are pathogenic bacteria growing in sputum and alveolar lavage fluid, which are identified as Nocardia otitidiscaviarum by Autof ms mass spectrometer. According to the results of pathogenic bacteria and the patient's condition, meropenem combined with compound sulfamethoxazole tablets were given anti-infection treatment, and the patient's condition improved and discharged. Conclusion　The clinical manifestations and imaging features of nocardiosis are lack of specificity, and are prone to misdiagnosis and missed diagnosis. Etiology is the key to disease diagnosis, and clinical examination and culture should be conducted in time.

0.05).However,the shorter the history of the disease,the better the efficacy of the treatment.The younger the patient was,the better the efficacy of the treatment.Conclusions Vulva dystrophy can be treated with focused ultrasound effectively and safely.This approach appears to be a new promising treatment method.

Objective To explore the application and effects of business process reengineering (BPR) in intravenous infusion center.Methods To analyze and compare retrospectively the effects before BPR with those after BPR for 240 cases.Results Infusion volume per day before BPR was (323.0±8.0) person,and (452.0 ±7.0) person after BPR,and the difference was significant (t =47.000,P＜0.01).Degree of satisfaction was 92.08％ before BPR and 98.75％ after BPR,the difference was statistically significant ( x2 =12.195,P＜0.05).Conclusions After nursing business reengineered in intravenous infusion center,nursing efficiency rates were increased and nursing defect rates,infection rates in hospital and nursing cost were significantly decreased,its worthy of spreading in the clinic.

Objective To explore the effect of evidence-based nursing during preoperative interview for abdominal surgery patients' recovery after general anesthesia. Methods 124 patients who accepted selective operation on abdominal region were randomly divided into evidence-based group and control group, 62 cases ineach group. On the base of routine method in the preoperative interview, the evidence-based group established evidence-based information supporting system, which aimed to dredge the psychological stress of the patients'response to surgery individually and intervene by demonstrating simulation of operation process. According to the traditional way, control group accepted routine preoperative interview. To compare patients' indexes of two groups before and after anesthesia. Results Patients' heart rate and the mean arterial pressure lower than control group before anesthesia in evidence-based group. The dosage of midazolam in evidence-based group was less than the control group (t =6. 73,7. 24,8. 17;P <0. 05). The difference of pre-anesthesia anxiety scores was statistically significant between evidence-based group and control group (t =7. 68 ,P <0. 01 ). But the difference of the anxiety scores after operation wasn' t statistically significant between two groups ( t = 0. 62, P > 0. 05 ). Patients in evidence-based group took shorter revival time than control group ( t = 9. 71,10. 45,11.32 ; P < 0. 01 ). Compared the different period pain score of two groups, patients of evidence-based group were lower than those of the control group, and the patients' satisfaction scores also were higher than those of control group, the differences were statistically significant (t =2. 38,5. 22,2. 63,7. 62;P <0. 05). Conclusions Application evidence-based nursing information support in preoperative interview effectively relieves the preoperative anxiety, can reduce the dosage of narcotic and sedative drugs, shorten the revival time, improve patient' s satisfaction and have reached the antieipant effects.

China ; epidemiology ; Congenital Hypothyroidism ; diagnosis ; epidemiology ; Humans ; Infant, Newborn ; Neonatal Screening

OBJECTIVETo investigate the effect of small interfering RNA (siRNA) targeting C-erbb-2 oncogene on the radiosensitivity of C-erbb-2-overexpressing lung adenocarcinoma cell line.

METHODSFour pairs of siRNA targeting the coding sequence of C-erbb-2 mRNA were synthesized and their interference effects were evaluated using quantitative real-time fluorescence PCR. The siRNA with the best interference effects was transfected into Calu-3 cells, which were then exposed to 2 or 5 Gy irradiation, with the cells with transfection or irradiation alone as the control groups. The cell apoptosis after the treatment was detected using annexin V-FITC Kit.

RESULTSThe apoptosis rate of the Calu cells was 7.767-/+0.551 in the blank group, 14.400-/+1.114 in the interference group, 11.867-/+0.737 in 2 Gy irradition group, 23.000-/+1.664 in 2 Gy irradiation + interference group, 16.100-/+0.624 in 5 Gy irradiation group, and 27.900-/+1.709 in 5 Gy irradiation+interference group.

CONCLUSIONThe siRNA targeting C-erbb-2 gene can enhance the radiosensitivity of Calu-3 cells to gamma-ray and increase their apoptosis rate following gamma-ray exposure in vitro.

Adenocarcinoma ; genetics ; pathology ; Apoptosis ; drug effects ; radiation effects ; Cell Line, Tumor ; Genes, erbB-2 ; genetics ; Humans ; Lung Neoplasms ; genetics ; pathology ; RNA Interference ; RNA, Small Interfering ; genetics ; Radiation Dosage ; Radiation Tolerance ; genetics

Background: The common reference intervals (RIs) for thyroid hormones currently used in China are provided by equipment manufacturers. This study aimed to establish thyroid hormone RIs in the population of Lanzhou, a city in the subplateau region of northwest China, and compare them with previous reports and manufacturer-provided values. Methods: In total, 3,123 individuals (1,680 men, 1,443 women) from Lanzhou, an iodine-adequate area of China, perceived as healthy were selected. The Abbott Architect analyzer was used to determine the serum concentration of thyroid hormones. The 95% RI was estimated using the 2.5th and 97.5th percentiles as the lower and upper reference limits, respectively. Results: The serum levels of thyroid-stimulating hormone (TSH), total triiodothyronine (TT3), antithyroglobulin (ATG) antibody, and antithyroid peroxidase (ATPO) antibody levels were significantly correlated with sex (P<0.05). TSH, total thyroxine (TT4), and ATPO levels were significantly correlated with age (P<0.05). The serum levels of TSH, ATG, and ATPO in men were significantly lower than in women; in contrast, the serum TT3 level was significantly higher in men than in women (P<0.05). Serum TSH, TT3, TT4, and ATG levels differed across age groups (P<0.05), but no such variation was observed for ATG levels (P>0.05). The established RIs of TSH, ATG, and ATPO in this study differed between sexes (P<0.05). The thyroid hormone RIs established herein were inconsistent with the manufacturer-provided values. Conclusion The RIs of thyroid hormones in the healthy population of Lanzhou were inconsistent with those in the manufacturer’s manual. Validated sex-specific values are required for diagnosing thyroid diseases.

Objective To investigate the effect of nursing student with different levels in practice in neonates intensive care unit(NICU)by different teaching patterns.Methods The nursing students graduated in 2007 and 2008 who achieved admission in practice in NICU were divided into two groups in retrospective analytical method.The nursing students were taught by the traditional teaching mode according to practice Outline.58 nursing students in group A were taught in different levels,so as to supply collective explanation,simulating demonstration,according to different educational background.56 nursing students in group B of different academic career were taught by the traditional teaching mode.Results There were differences among the two groups about the theory knowledge,technical skill,the time of mastering basic technical skill,the time of aiding skills,and the differences were obvious(P<0.01),The score of mixed ability and teaching satisfaction ratio were markedly increased compared with group B.Conclusions The different teaching patterns established the balcony for nursing students symposium,and enhanced nursing students'theory skins and mixed ability,shortened the instructed time,and also increased teaching efficiency and quality of care.And also improved activeness of observing the state of an illness and the level of expertise.and achieved the desired result.

Objective To explore the clinical effects of calcium folinate(CF)rescue on high-dose methotrexate (HDMTX)therapy at different times.Methods Children with acute lymphocytic leukemia(ALL)who met the inclusion criteria were randomly divided into two groups in line with chemotherapy order:observation group(Group A)and control group(Group B).Chemotherapy regimens included remission induction chemotherapy(VDLD),consolidation therapy(CAT),shelters to prevent(HDMTX+CF),reinduction therapy.Group A was intravenously injected HDMTX for 24 hours and then rescued by CF for 12hours.Group B was intravenously injected HDMTX for 6 hours and then rescued by CF for 30 hours.Side effects after administration of medicine were observed,in particular,the gastrointestinal tract reaction,which included nausea and vomiting,oral mucous membrane reaction,leukocyte reaction,the toxicity of the heart,liver,kidney,nerve and response durations.Results There were significant differences in both groups on response degree in the gastrointestinal tract reaction,oral mucous membrane reaction,and leukocyte reaction (P<0.01,P<0.05);while there was significant difference in response duration after chemotherapy for both groups(P<0.01).There was no significant difference in the heart,liver,kidney,neurological toxicity reaction(P>0.05).Conclusions There are differences in side effects of CF rescue on HDMTX therapy at different times.Both groups could reduce the toxic reaction,but the protocol that gives HDMTX for 24 hours and then rescues by CF for 12 hours is more conducive to repair the normal tissue cell's damage,improve chemotherapy efficacy and children's quality of life,with a better clinical value.

Objective To investigate effect of global nursing care on rehabilitation effect of gynecologic cancer patients receiving laparoscopic surgery.Methods 115 cases of laparoscopic surgery in gynecologic cancer patients were randomly divided into two groups.the intervention group is 58 and the control group 57.Two groups with the laparoscopic surgery received a same routine care,in the intervention group patients received an intervention care consisted of cognitive behavioral intervention before surgery,postoperative pain,intervention and follow-up intervention after discharge as one of the whole individual nursing intervention model;In the control patients received traditional routine care before and after laparoscopic surgery.Intervention effects were observed in two groups.Results The intervention group at different times after the pain visual analogue scale (VAS) was significantly lower than the control group.72 h VAS in intervention group was ( 1.35 ±1.02),and was (2.76 ± 1.20) in control group,and the difference was significant ( t =6.79,P ＜ 0.01 ) ;days in hospital in intervention group was (7.15 ±2.15)d and (9.89 ±2.18) in control group,and the difference was significant ( t =6.79,P ＜ 0.01 ) ; The recurrence rate in intervention group ( 1.72％ ) was significantly lower than the control group ( 14.03％ ),and the difference was significant ( x2 =4.45,P ＜0.05).Conclusions Whole course of individualized nursing intervention care has promoted rehabilitation,increased the postoperative quality of life and improve the effect of laparoscopic surgery of patients.