1.Effects of vagotomy on UCP2 mRNA expression and gastric acid secretion in rats.
Yuan ZOU ; Mei YANG ; De-Zheng GONG ; Li-Li GUAN ; Nan TIAN
Chinese Journal of Applied Physiology 2005;21(3):290-292
AIMThe relationship between gastric acid secretion and ATP level, and regulation of uncoupling protein 2 (UCP2) mRNA expression by vagus nerve were studied in vagotomies rats.
METHODSWith the high selective vagotomy model, the gastric acidity was titrated to pH 7.0 with 0.1 mol/L NaOH solution and ATP contents were quantified by using fluorimetry. The expression of UCP2 mRNA was observed by using Northern blot in stomach of rats.
RESULTSBoth of gastric acidity and ATP contents in stomach body decreased significantly at 24 h after vagotomy. The expression of UCP2 mRNA was markedly increased as compared with sham operation group.
CONCLUSIONATP contents decreased and vagus nerve down-regulates expression of UCP2 mRNA in stomach corpus in vagotomies rats. The results indicates that vagus nerve could underlay the gastric acidity by inhibiting expression of UCP2 mRNA and increasing ATP contents in rats.
Adenosine Triphosphate ; metabolism ; Animals ; Gastric Acid ; secretion ; Ion Channels ; genetics ; metabolism ; Male ; Mitochondrial Proteins ; genetics ; metabolism ; RNA, Messenger ; genetics ; Rats ; Rats, Sprague-Dawley ; Uncoupling Protein 2 ; Vagotomy ; Vagus Nerve ; metabolism
2.Establishment of human lung squamous carcinoma cell line CHLH-1
Hong-Cheng LIU ; Sheng-Dong HUANG ; De-Jun GONG ; Xiao-Hong LIU ; Yang YUAN ; Zhi-Yun XU ;
Academic Journal of Second Military Medical University 1999;0(12):-
Objective:To establish a human lung squamous carcinoma cell line and to study its biological characteristics. Methods:Lung squamous carcinoma specimens were freshly resected during operation;the tissues were incubated in vitro and the cell line was named CHLH-1.The biological characteristics of the cells were studied by light microscopy,electron microsco- py,chromosome analysis and transplantation experiment.Results:Cells from the specimens of the primary tumor,the CHLC- 1 cell line and the cells from transplanted tumor possessed the characteristics of malignant squamous epithelium under light and electron microscope.The cell growth curve,doubling time and mitotic index were also observed in vitro.Nuclear chromosome analysis revealed that the tumor was a subtriploid with a mode of 60-68 per cell.Tumor nodes were observed under the skin of nude mice by heterogenic transplantation.Conclusion:The characteristics of the established cell line suggest that it is a newly established human squamous carcinoma cell line.
3.Hybrid external fixator combined with limited internal fixation for the treatment of high-energy tibial plateau fractures.
De-fu YANG ; Gong-lin ZHANG ; Ming ZHANG ; Li-ping XIA ; Ji-hua HE ; Jun-yang PENG
China Journal of Orthopaedics and Traumatology 2009;22(3):219-220
Adult
;
Aged
;
External Fixators
;
Female
;
Fracture Fixation, Internal
;
methods
;
Humans
;
Male
;
Middle Aged
;
Tibial Fractures
;
surgery
4.Correlation between Residual Strength of Neck and Shoulder Muscles and Respiratory Function in Patients with Cervical Spinal Cord Injury
liang Ming YANG ; Li TANG ; gang De YANG ; Liang CHEN ; ming Hui GONG ; jun Jian LI
Chinese Journal of Rehabilitation Theory and Practice 2017;23(11):1308-1312
Objective To investigate the correlation between residual strength of neck and shoulder muscles and respiratory function in patients with cervical spinal cord injury.Methods From January,2015 to June,2016,the muscle strength of sternocleidomastoid,trapezius and deltoid was tested in 30 patients with cervical spinal cord injury five and 24 weeks after injury.Meanwhile,their neurological and pul-monary function was evaluated.Results There was correlation between the vital capacity and muscle strength of deltoid both five and 24 weeks after injury(r>0.806,P<0.05)in the patients intermittent without ventilation,and it was found in sense score five weeks after injury (r=0.914,P<0.01),motor score(r=0.979,P<0.001)and the muscle strength of trapezius(r=0.894,P<0.01)24 weeks after injury.Conclu-sion The residual strength of neck and shoulder muscles,especially of deltoid,plays an important role in the respiratory function in patients with cervical spinal cord injury.
5.Significance of serum neuron-specific enolase in patients with acute traumatic brain injury.
Wei GUAN ; Yi-lin YANG ; Wei-min XIA ; Lu LI ; De-sheng GONG
Chinese Journal of Traumatology 2003;6(4):218-221
OBJECTIVETo study the association between serum neuron-specific enolase (NSE) and the extent of brain damage and the outcome after acute traumatic brain injury (TBI).
METHODSThe release patterns of serum NSE in 78 patients after acute TBI were analyzed by using the enzyme linked immunosorbent assay. The levels of NSE were compared with Glasgow coma scale, the category of brain injury and the outcome after 6 months of injury.
RESULTSThere were different NSE values in patients with minor (12.96 microg/L+/-2.39 microg/L), moderate (23.44 microg/L+/-5.33 microg/L) and severe brain injury (42.68 microg/L+/-4.57 microg/L). After severe TBI, the concentration of NSE in patients with epidural hematomas was 13.38 microg/L+/-4.01 microg/L, 24.03 microg/L+/-2.85 microg/L in brain contusion without surgical intervention group, 55.20 microg/L+/-6.35 microg/L in brain contusion with surgical intervention group, and 83.85 microg/L+/-15.82 microg/L in diffuse brain swelling group. There were close correlations between NSE values and Glasgow coma scale (r=-0.608, P<0.01) and the extent of brain injury (r=0.75, P<0.01). Patients with poor outcome had significantly higher initial and peak NSE values than those with good outcome (66.40 microg/L+/-9.46 microg/L, 94.24 microg/L+/-13.75 microg/L vs 32.16 microg/L+/-4.21 microg/L, 34.08 microg/L+/-4.40 microg/L, P<0.01, respectively). Initial NSE values were negatively related to the outcome (r=-0.501, P<0.01). Most patients with poor outcomes had persisting or secondary elevated NSE values.
CONCLUSIONSSerum NSE is one of the valuable neurobiochemical markers for assessment of the severity of brain injury and outcome prediction.
Acute Disease ; Adolescent ; Adult ; Aged ; Aged, 80 and over ; Brain Injuries ; enzymology ; Child ; Humans ; Middle Aged ; Phosphopyruvate Hydratase ; blood ; Whole-Body Irradiation
7.The causes and strategies for the postoperative shortening in distal radius fractures.
De-fu YANG ; Gong-lin ZHANG ; Li-ping XIA ; Yu HUI ; Jian-hua HUANG ; Jian-feng CHEN
China Journal of Orthopaedics and Traumatology 2010;23(8):581-584
OBJECTIVETo analyze the cause of the postoperative shortening in distal radius fractures and to find treatment strategies to enhance the reduction effect of distal radius fracture and long-term efficacy.
METHODSFrom June 2005 to January 2009, 46 patients with distal radius fractures were treated by surgical operation including 17 males and 29 females,ranging from 27 to 90 years old with an average age of 48 years. All fractures were reduced satisfactory with smooth articular surface. The postoperative radial shortening was analyzed.
RESULTSAll patients were followed-up for 6 to 24 months with an average of 14 months. Among them, 12 patients had radial shortening. The causes of postoperative radial shortening includes: (1) patients older than 60 years; (2) severe osteoporosis; (3) preoperative displacement and comminuted fractures; (4) inappropriate fixation methods; (5) inadequate bone graft; (6) premature load.
CONCLUSIONThe key points to enhance the treatment outcomes include precise judgement of the fracture type and bone quality, sufficient bone graft, firmly fixed after anatomical reduction and an appropriate plan for early loadless functional exercise. Traumatic arthritis may be avoided or delayed if the above-mentioned six causes can be taken into consideration or preventive measures can be taken.
Adult ; Age Factors ; Aged ; Aged, 80 and over ; Bone Transplantation ; Female ; Follow-Up Studies ; Fracture Fixation ; Humans ; Male ; Middle Aged ; Osteoporosis ; etiology ; Postoperative Complications ; etiology ; therapy ; Radius ; pathology ; Radius Fractures ; surgery
8.Effects of Goutengsan on model of Alzheimer dementia in rats by AlCl3.
Hou-Cai HUANG ; Rong-Ling ZHONG ; Peng CAO ; Chun-Ru WANG ; De-Gong YANG
China Journal of Chinese Materia Medica 2008;33(5):553-556
OBJECTIVEObserve the effects of Goutengsan on SOD, MAO-B, GSH-PX, NO, LDH, index of brain, rate of death and so on in rats to study therapeutic effects and mechanism of Goutengsan on Alzheimer dementia (AD) model.
METHODOne hundred and twenty rats were randomly divided into 6 groups, 3 experimental groups of which were daily administrated with Goutengsan extract whereas the model and control groups were given NS (0.01 mL x g(-1)). Aniracetam at 0.1 g x kg(-1) served as a positive control. At the 5th day after administration, all groups except the control were administrated (ip) with AlCl3 (100 mg x kg(-1) ) for successive 50 days at 1 day interval. After administration, the death rate, body weight, training scores, brain index, MAO-B, SOD, GSH-Px in brain and NO, LDH in serum were determined.
RESULTThe brain index, SOD, GSH-Px activities as well as NO content of drug-treated groups were strikingly higher that of model group, and had not obvious difference from that of normal group except content of LDH was higher.
CONCLUSIONGoutengsan could increase the brain index, cut down the rate of death, stable increase of body weight, promote the endogenous antioxidant activity, enhance the clearance of lipid peroxide and other metabolic waste, inhibit the MAO-B activity, reduced the leakage of LDH and maintain the content of NO at a normal level. Therefore Goutengsan could protect cells, delay senile, improve symptoms of AD.
Aluminum Compounds ; pharmacology ; Alzheimer Disease ; chemically induced ; drug therapy ; metabolism ; Animals ; Body Weight ; drug effects ; Brain ; drug effects ; metabolism ; Chlorides ; pharmacology ; Disease Models, Animal ; Drugs, Chinese Herbal ; pharmacology ; Female ; Glutathione Peroxidase ; metabolism ; Lipid Peroxidation ; drug effects ; Male ; Malondialdehyde ; metabolism ; Memory ; drug effects ; Nitric Oxide ; metabolism ; Oxidative Stress ; drug effects ; Pyrrolidinones ; pharmacology ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Superoxide Dismutase ; metabolism
9.The ion targets of arrhythmias induced by ouabain and aconitine in guinea pig and rat ventricular myocytes.
Dong-mei GONG ; Hong-li SHAN ; Yu-hong ZHOU ; De-li DONG ; Bao-feng YANG
Acta Pharmaceutica Sinica 2004;39(5):328-332
AIMTo observe the effects of ouabain and aconitine on APD and ion channels in isolated guinea pig and rat ventricular myocytes; to elucidate the action mechanisms of these two drugs and set up new arrhythmic models on cellular level.
METHODSIn isolated ventricular myocytes of guinea pig and rat, the effects of ouabain and aconitine on APD, ICa-L, Ik, Ito and Ik1 were observed using the whole cell patch clamp technique.
RESULTSOuabain (5 micromol x L(-1)) obviously prolonged the APD90, increased ICa-L, decreased Ik and Ik1 in guinea pig ventricular myocytes. Aconitine (1 micromol x L(-1)) lengthened the APD90, increased ICa-L, decreased Ito and increased Ik1 in rat ventricular myocytes.
CONCLUSIONThe targets on ouabain- and aconitine-induced arrhythmias included APD, ICa-L, Ik, Ito, and Ik1. APD, ICaL, Ik and Ito must be the powerful ones, both in arrhythmic and antiarrhythmic courses. The ouabain- and aconitine- induced arrhythmic models on cellular level were built to study the antiarrhythmic mechanisms of chemicals and evaluate new drugs. These two new-type models in vitro were stable, liable, repeatable and economic, which were superior to those typical models in vivo.
Aconitine ; pharmacology ; Action Potentials ; drug effects ; Animals ; Arrhythmias, Cardiac ; pathology ; physiopathology ; Calcium Channels, L-Type ; drug effects ; Cell Separation ; Female ; Guinea Pigs ; Heart Ventricles ; pathology ; Male ; Myocytes, Cardiac ; metabolism ; physiology ; Ouabain ; pharmacology ; Potassium Channels, Inwardly Rectifying ; drug effects ; Rats ; Rats, Wistar
10.Gene transfection efficiency and destination of recombinant adenovirus gene transfer through internal jugular vein injection in rats.
Yu-hang AI ; Li-na ZHANG ; Hua GONG ; Liu PENG ; Yang-de ZHANG
Journal of Central South University(Medical Sciences) 2005;30(6):653-656
OBJECTIVE:
To investigate the adenovirus-mediated LacZ gene expression and the destination in different organs of SD rats after the intravenous injection in rats.
METHODS:
Recombinant adenovirus vector containing LacZ was transferred to SD rats by injecting into the internal jugular vein. To identify the sites and periods of LacZ gene expression, X-gal staining was used to detect beta-gal level and period of LacZ gene expression of different organs in the transfected and non-transfected rats at different time intervals.
RESULTS:
On the 1st day after the injection, the lung, liver, kidney, and spleen expressed some beta-gal; on the 3rd day after the injection, the lung, liver, kidney, and spleen expressed beta-gal obviously; their peak levels were on the 7th day; the beta-gal level decreased on the 14th day; beta-gal expression disappeared in the most organs except the lungs on the 28th day. In all animals, the brain did not express any beta-gal.
CONCLUSION
The adenovirus-mediated exogenous gene transfer in the internal jugular vein may be an effective approach of gene therapy in some diseases in the lung, liver, and kidney.
Adenoviridae
;
genetics
;
Animals
;
Female
;
Gene Transfer Techniques
;
Genetic Therapy
;
Injections, Intravenous
;
Jugular Veins
;
Lac Operon
;
genetics
;
Lung
;
metabolism
;
Male
;
Random Allocation
;
Rats
;
Rats, Sprague-Dawley
;
Recombinant Proteins
;
genetics
;
Transfection