1. Therapeutical effect of liraglutide treatment in a mouse model of allergic rhinitis
Journal of Shanghai Jiaotong University(Medical Science) 2019;39(5):487-493
Objective • To investigate whether intraperitoneal injection of liraglutide can improve the inflammatory state of allergic rhinitis (AR) in mice and provide new treatment options for AR. Methods • Eighteen BALB/c mice (SPF grade) were divided into three groups (control group, AR group and treatment group) by random number table method. The AR and treatment group were established through intraperitoneal injection of OVA and aluminum hydroxide (replaced by physiological saline in control group). And treatment group received intraperitoneal injection of liraglutide (replaced by physiological saline in control and AR groups). The changes in the numbers of sneezing and nose scratching of mice were observed after the intervention of liraglutide. The number of eosinophil infiltration, goblet cell proliferation and mucosal thickness in mice nasal mucosa were measured. Enzyme-linked immunosorbent assays were used to detect the concentrations of OVA-specific IgE (OVA-sIgE), IL-4 and IL-5 in serum of mice. Results • In control group, there were rare eosinophils and goblet cell hyperplasia. In AR group, obvious goblet cell hyperplasia, significant eosinophil infiltration and thickening mucosal were observed. The number of eosinophil, goblet cell hyperplasia, and mucosal thickness in AR group significantly increased compared with those in control group (all P<0.05). The numbers of nose scratching and sneezing in AR group were significantly higher than those in the control group (both P<0.05), and the above symptoms in treatment group were improved compared with AR group (both P<0.05). The serum concentrations of OVA-sIgE, IL-4 and IL-5 in AR group were significantly higher than those in control group (all P<0.05), and the above indicators in treatment group were lower than those in AR group (all P<0.05). Conclusion • Intraperitoneal injection of liraglutide can effectively improve the symptoms and inflammatory level of AR in mice, which may be a novel research direction in the treatment of AR.
2.Current situation researching of methylation in tumor.
Di SHAO ; An-fang CUI ; Liu-luan ZHU ; Ai-jun QIAO ; Xing-xing KONG ; Xiao-jun LIU ; Yong-sheng CHANG ; Fu-de FANG
Acta Academiae Medicinae Sinicae 2009;31(6):786-790
The disorders of DNA and histone methylation have a close relationship with the development and progression of tumors. Epigenetic regulation is critical in maintaining the stability and integrity of the expression profiles of different cell types by modifying DNA methylation and histone methylation. However, the abnormal changes of methylation often result in the development and progression of tumors. This review summarized the theory of tumor genomic and histone methylation, detection methods of methylation and their applications, and the clinical application of methylation as biological markers and drug targets.
DNA Methylation
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Histones
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metabolism
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Humans
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Methylation
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Neoplasms
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genetics
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metabolism
3.Research advances in Sirt1 gene.
Ai-jun QIAO ; Jin ZHIAO ; Xiao-jun LIU ; Di SHAO ; Liu-luan ZHU ; An-fang CUI ; Xing-xing KONG ; Fu-de FANG ; Yong-sheng CHANG
Acta Academiae Medicinae Sinicae 2009;31(6):782-785
As the most homologic homologue of silent information regulator 2 of yeast, Sirt1 gene is extensively expressed in mature tissues, and is rich in early embryo and reproductive cells. It is involved in the regulation of gene transcription, energy metabolism and cell aging. It promotes fat mobilization in adipocytes and glucose production in liver and regulates insulin secretion in islet beta cell. Furthermore, Sirt1 gene is an essential endogenous apoptosis inhibitor. In future, it may be used as new drug targets or applied in other disease management modalities.
Animals
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Humans
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Sirtuin 1
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genetics
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metabolism
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physiology
4.Expression of Bcl-2 and Bax proteins in myocardial cells after Macleaya cordata alkaloids poisoning in rats.
De-Yu ZHANG ; Qing-Quan KONG ; Chun-Bing ZHANG ; Jian-Hua ZHU ; Ming-Di CHEN ; Peng WANG
Journal of Forensic Medicine 2009;25(4):246-253
OBJECTIVE:
To observe the expression of Bcl-2 and Bax proteins in rat's myocardial cells after Macleaya cordata alkaloids poisoning, and to provide certain molecular biology references for the detection of Macleaya cordata alkaloids poisoning.
METHODS:
Experimental model of Macleaya cordata alkaloids poisoning was established, the expression levels of Bcl-2 and Bax proteins in these cells were detected by immunohistochemistry, and the results were analyzed by computer image system.
RESULTS:
The expression levels of Bcl-2 and Bax proteins in myocardial cells in poisoning groups were much greater than those in the control groups (P<0.05).
CONCLUSION
If the clinical symptoms may not be obvious, the detection of Bcl-2 and Bax proteins level by immunohistochemistry still could be ancillary method.
Animals
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Disease Models, Animal
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Female
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Immunohistochemistry
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Male
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Myocardium/pathology*
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Myocytes, Cardiac/pathology*
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Papaveraceae/chemistry*
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Papaverine/poisoning*
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Proto-Oncogene Proteins c-bcl-2/metabolism*
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Rats
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Rats, Sprague-Dawley
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Staining and Labeling
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Time Factors
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bcl-2-Associated X Protein/metabolism*
5.Clinicopathological Features and Long-Term Prognostic Role of Human Epidermal Growth Factor Receptor-2 Low Expression in Chinese Patients with Early Breast Cancer:A Single-Institution Study
Qing Zi KONG ; Qun Li LIU ; Qin De HUANG ; Tong Yu WANG ; Jie Jing LI ; Zheng ZHANG ; Xi Xi WANG ; Ling Chuan LIU ; Di Ya ZHANG ; Kang Jia SHAO ; Min Yi ZHU ; Meng Yi CHEN ; Mei LIU ; Hong Wei ZHAO
Biomedical and Environmental Sciences 2024;37(5):457-470
Objective This study aimed to comprehensively analyze and compare the clinicopathological features and prognosis of Chinese patients with human epidermal growth factor receptor 2(HER2)-low early breast cancer(BC)and HER2-IHC0 BC. Methods Patients diagnosed with HER2-negative BC(N=999)at our institution between January 2011 and December 2015 formed our study population.Clinicopathological characteristics,association between estrogen receptor(ER)expression and HER2-low,and evolution of HER2 immunohistochemical(IHC)score were assessed.Kaplan-Meier method and log-rank test were used to compare the long-term survival outcomes(5-year follow-up)between the HER2-IHC0 and HER2-low groups. Results HER2-low BC group tended to demonstrate high expression of ER and more progesterone receptor(PgR)positivity than HER2-IHC0 BC group(P<0.001).The rate of HER2-low status increased with increasing ER expression levels(Mantel-Haenszel χ2 test,P<0.001,Pearson's R=0.159,P<0.001).Survival analysis revealed a significantly longer overall survival(OS)in HER2-low BC group than in HER2-IHC0 group(P=0.007)in the whole cohort and the hormone receptor(HR)-negative group.There were no significant differences between the two groups in terms of disease-free survival(DFS).The discordance rate of HER2 IHC scores between primary and metastatic sites was 36.84%. Conclusion HER2-low BC may not be regarded as a unique BC group in this population-based study due to similar clinicopathological features and prognostic roles.