1.Berberine inhibited apoptosis of human umbilical vein endothelial cells induced by Staphylocoocus aureus: an experimental research.
Chuan-yin XIONG ; Yong-hang FU ; Han-bin HU ; Ai-fen BI ; De-cui PEI
Chinese Journal of Integrated Traditional and Western Medicine 2014;34(6):710-713
OBJECTIVETo study the inhibition of berberine (BBR) against ECV-304 apoptosis induced by Staphylococcus aureus (S. aureus).
METHODSECV-304 cells were pre-treated with 128 microg/mL BBR for 2 h and then S. aureus was added (1:100). The viability of cells was detected by MTT (3-4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The morphological changes were observed by Hoechst 33258 staining. The protection of BBR for infected cells was detected by DNA Ladder.
RESULTSECV-304 cells' viability were not obviously affected by berberine. But S. aureus induced ECV-304 cells' viability could be significantly inhibited by pre-treatment of BBR (P < 0.05). Besides S. aureus-induced ECV-304 apoptosis could be reduced, with significantly lessened apoptotic body and unobvious DNA degradation.
CONCLUSIONBBR could significantly inhibit S. aureus induced ECV-304 apoptosis.
Apoptosis ; drug effects ; Berberine ; pharmacology ; Cell Line ; Human Umbilical Vein Endothelial Cells ; drug effects ; microbiology ; pathology ; Humans ; Staphylococcus aureus
2.Asymmetric crying facies: report of five cases.
Xiao-Ping HU ; Juan CHEN ; Bin XIA ; De-Zhi MU
Chinese Journal of Contemporary Pediatrics 2008;10(2):259-260
Abnormalities, Multiple
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Crying
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Facial Asymmetry
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genetics
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Female
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Humans
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Infant, Newborn
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Male
3.Expression and role of nuclear transcription factor Sp1 in macrophages stimulated by silicon dioxide.
Jin-sheng WANG ; Qing-fu ZENG ; De-yun FENG ; Yong-bin HU ; Ji-fang WEN
Chinese Journal of Industrial Hygiene and Occupational Diseases 2006;24(9):518-522
OBJECTIVETo study the expression and localization of nuclear transcription factor Sp1 in macrophages after stimulated by silicon dioxide in vivo and in vitro.
METHODSForty Sprague Dawley rats were randomly divided into the control group and the silica exposure group, 20 in each group. The rat silicosis models were established by direct tracheal instillation of silica into rat lung (0.2 g/kg) only once while the control group was instilled with equal amount of saline. Animals were killed at 1st, 7th, 14th, 21st and 28th day after instillation. Dynamic changes of Sp1 protein expression and its cellular localization were detected by immunohistochemistry in pulmonary macrophages. In vitro, Sp1 mRNA and protein expression and their dynamic changes were monitored by RT-PCR and western blotting after stimulated by silicon dioxide in cultured RAW264.7 macrophages respectively. Cellular localization of Sp1 protein was characterized by immunocytochemistry.
RESULTSCompared to the control group, the Sp1 protein expression was increased in pulmonary macrophages and reached the peak at the 14th day in the silica exposure group. In vitro, the Sp1 mRNA level began to rise at 30 minutes after the administration of silicon dioxide and reached the peak at 240 minutes and then decreased to the minimal level at 960 minutes. The Sp1 total protein and nuclear protein also exhibited the similar trend. The former reached the peak at 240 minutes and the latter at 480 minutes. The significant nuclear translocation of Sp1 protein was observed at 120 minutes after the administration of silicon dioxide and became most significant at 480 minutes.
CONCLUSIONSilicon dioxide can activate nuclear transcription factor Sp1 in macrophages in vivo and in vitro. Sp1 might play an important pathogenic role in the development of silicosis.
Animals ; Cells, Cultured ; Gene Expression Regulation ; drug effects ; Immunohistochemistry ; Macrophages, Alveolar ; drug effects ; metabolism ; Macrophages, Peritoneal ; drug effects ; metabolism ; Male ; RNA, Messenger ; genetics ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Reverse Transcriptase Polymerase Chain Reaction ; Silicon Dioxide ; pharmacology ; Sp1 Transcription Factor ; biosynthesis ; genetics
4.Evaluation of peripherally inserted central catheters in high risk newborns.
Bin XIA ; Ying XIONG ; Yan-Ling HU ; De-Zhi MU
Chinese Journal of Contemporary Pediatrics 2009;11(2):100-103
OBJECTIVETo evaluate the feasibility and safety of the peripherally inserted central catheters (PICC) as a venous access for newborns who need a long-term venous transfusion.
METHODSSixty-five newborns receiving PICC and 80 newborns receiving peripheral intravenous catheters (PIV) from April 2006 to February 2008 were included in this study. A retrospective cohort study was used to compare the indwelling time of catheters, catheter-related mechanical complications, the incidence of sepsis, and the mortality between the two groups.
RESULTSThe indwelling time of catheters in the PICC and the PIV groups was 18.75+/-7.62 days (range:7-62 days) and 1.49+/-0.57 days (range: 30 minutes to 4 days) respectively. The indwelling time of catheters in the PICC group was significantly longer than that in the PIV group (<0.01). The incidence of catheter-related mechanical complications in the PICC group was significantly lower than that in the PIV group (27.7% vs 63.8%; <0.01). There were no significant differences in the incidence of sepsis and the mortality between the two groups.
CONCLUSIONSThe application of PICC can cause a decrease in the number of venous puncture. PICC is a safe and effective venous access in newborns.
Catheterization, Central Venous ; adverse effects ; Catheterization, Peripheral ; adverse effects ; Humans ; Infant, Newborn ; Sepsis ; etiology ; Time Factors
5.AP-1 regulates TGF-beta1-induced secretion of Type I collagen in human lung fibroblasts.
Yong-bin HU ; Qing-fu ZENG ; De-yun FENG ; Xiang LI ; Jin-wu PENG
Journal of Central South University(Medical Sciences) 2007;32(5):776-781
OBJECTIVE:
To investigate the role of AP-1 in the secretion of Type I collagen in TGF-beta1-stimulated human lung fibroblasts.
METHODS:
Human lung fibroblasts cell line (HLF-02) was cultured, and then stimulated with 10 microg/L TGF-beta1 at different time points. Curcumin was added into the culture medium to inhibit the AP-1 activity before incubating with TGF-beta1. AP-1 DNA binding activity was assayed by electrophoretic mobility shift assay (EMSA), and the expression of Type I collagen was detected by Western blot and RT-PCR.
RESULTS:
TGF-beta1 could induce the transcription and secretion of Type I collagen in HLF-02 cells(P<0.05). TGF-beta1 could upregulate the AP-1 DNA binding activity ( P<0.05). Curcumin ( 5, 10, 15, and 20 micromol/L) could inhibit the AP-1 DNA binding activity in TGF-beta1-stimulated cells (the inhibition ratio was 17.1%, 17.6%, 24.2%, and 31.3%; P<0.05). Curcumin (5, 10, 15, and 20 micromol/L) could also inhibit the secretion of Type I collagen significantly (the inhibition ratio was 62.1%, 58.8%, 62.1%, and 59.6%; P<0.05).
CONCLUSION
AP-1 is responsible for the secretion of TGF-beta1-induced Type I collagen in human lung fibroblasts.
Cell Line
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Collagen Type I
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metabolism
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Fibroblasts
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cytology
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drug effects
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metabolism
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Humans
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Lung
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cytology
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Signal Transduction
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Transcription Factor AP-1
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metabolism
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Transforming Growth Factor beta1
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pharmacology
6.Clinical and pathological analysis of chronic rejection following orthotopic liver transplantation.
Yi MA ; Xiao-shun HE ; Rui-de HU ; Guo-dong WANG ; An-bin HU ; Qiang TAI ; Xiao-feng ZHU
Chinese Journal of Surgery 2010;48(4):288-292
OBJECTIVETo investigate the clinical manifestation and pathological features of chronic rejection (CR) and the management of CR after orthotopic liver transplantation (OLT).
METHODSFrom January 2004 to December 2006, there were 516 patients who had undergone OLT. All the clinical and pathological data were collected and retrospectively studied. Clinical manifestation, histopathological feature, diagnosis and anti-rejection treatment of CR were summarized and analyzed.
RESULTSThe incidence of CR was 2.3% (12/516), including 7 cases with early phases of CR and 5 cases with late phases of CR. The main pathological changes of CR were the vanishing bile duct syndrome and obliterative arteriopathy;and the early stage of CR were the damage of inter lobular bile duct, necrotic inflammation in central lobule, and inflammatory cells infiltration in portal area. Among 12 patients with CR, 7 cases with early CR were reversed by methylprednisolone (MP) pulse treatment and adjusting immunosuppressant dose, including 2 cases of whom were prescribed OKT3 treatment and 2 cases treated by ATG, and 5 cases with late CR underwent liver retransplantation (re-LT). Two patients died from infection, 1 case died from multiple organ failure in perioperative period after re-LT, another 2 cases were cured by re-LT, and the CR related mortality was 25.0% (3/12).
CONCLUSIONSChronic rejection following OLT is lack of typical clinical manifestation and pathological features, and the pathological changes can overlap and coexist. Post-transplant liver biopsy and graft specimen after re-LT is still "gold standard" to CR diagnosis. Some of early CRs can be reversed by early diagnosis and early treatment; for late CR recipient, re-LT should be considered.
Adolescent ; Adult ; Aged ; Child ; Chronic Disease ; Female ; Graft Rejection ; diagnosis ; pathology ; therapy ; Humans ; Liver Transplantation ; Male ; Middle Aged ; Retrospective Studies ; Young Adult
7.Incorporation of TV regularization in deconvolution for partial volume correction in PET imaging.
De-Bin HU ; Li-Jun LU ; Yuan-Yuan GAO ; Hou-Jin ZHANG ; Yan-Jiang HAN ; Cheng-Wei GU ; Jian-Hua MA
Journal of Southern Medical University 2015;35(4):474-480
We propose a method using total variation (TV) regularization in deconvolution for partial volume correction in PET imaging. In the degraded image model, we used TV regularization procedure in Van Cittert (VC) and Richardson-Lucy (RL) deconvolution algorithms. These methods were tested in simulated NCAT images and images of NEMA NU4-2008 IQ phantom and tumor-bearing mouse scanned by Simens Invoen microPET. The simulated experiment and tumor-bearing mouse experiment showed that the algorithms using TV regularization provided superior qualitative and quantitative appearance compared with traditional VC and RL algorithms. When the mean intensity of the tumor increased by (10±1.8)%, the SD increase percentage was decreased from 49.98% to 14.26% and from 42.76% to 4.70%, suggesting the efficiency of the proposed algorithms for reducing PVEs in PET.
Algorithms
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Animals
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Image Processing, Computer-Assisted
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Imaging, Three-Dimensional
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Mice
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Phantoms, Imaging
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Positron-Emission Tomography
8.Isolated non-compaction of ventricular myocardium in a victim of the Wenchuan earthquake with crush syndrome and acute renal failure.
Fang LIU ; Fa-bao GAO ; Ping FU ; Hong-yu QIU ; Hong-de HU ; Hong TANG ; Ling ZHANG ; Bin SONG ; Wan-xin TANG ; Ye TAO ; Song-min HUANG
Chinese Medical Journal 2009;122(18):2196-2198
9.Effects of silica on the expression of plasminogen activator inhibitor-1 and activator protein-1 in human alveolar epithelial cells type II.
Zhi LIN ; Yong-bin HU ; De-Yun FENG ; Ling CHU ; Jin-sheng WANG ; Qing-fu ZENG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2005;23(5):355-358
OBJECTIVETo investigate the mechanism of silicosis by observing the effects of silica on the expression of plasminogen activator inhibitor-1 (PAI-1) and activator protein-1 (AP-1) in human alveolar epithelial cells type II (A549).
METHODSA549 cell and SiO(2) (200 microg /ml) were co-cultured for 0, 4, 8, 16 and 24 h respectively. The reverse transcriptase polymerase chain reaction (RT-PCR), Western blotting and SP immunocytochemistry were used for detections of the PAI-1 mRNA and protein expression. The nucleoprotein and total protein expression of AP-1 were investigated by Western blotting.
RESULTSThe expression levels of PAI-1 mRNA and protein were increased in a time-dependent manner(r(mRNA) = 0.911, r(protein) = 0.902, P < 0.05). The expressions of PAI-1 mRNA and protein in experimental groups were higher than that in control group (P < 0.05) and was the highest in 24 h group [(0.73 +/- 0.01) vs (0.36 +/- .03)]. The nucleoprotein expressions of c-jun/c-fos in experimental groups were also higher than in control group (P < 0.05), and the nucleoprotein expression level of c-jun was the highest in 4 h group [(1.54 +/- 0.02) vs (0.56 +/- 0.03)]; the nucleoprotein expression level of c-fos was the highest in 8 h group [(0.36 +/- 0.01) vs (0.15 +/- 0.01)]. Both c-jun and c-fos expression were decreased after 16 h, but the total protein expression of c-jun/c-fos had no difference in all experimental groups. The positive signal of PAI-1 was located in cytoplasm and nucleus.
CONCLUSIONSiO(2) could induce PAI-1 expression of A549 in a time-dependent manner, and AP-1 activation can be observed in early time.
Alveolar Epithelial Cells ; drug effects ; metabolism ; Cell Line ; Humans ; Plasminogen Activator Inhibitor 1 ; metabolism ; Silicon Dioxide ; toxicity ; Transcription Factor AP-1 ; metabolism
10.Osteogenic potential of the human bone morphogenetic protein 2 gene activated nanobone putty.
Xiao-bin TIAN ; Li SUN ; Shu-hua YANG ; Yu-kun ZHANG ; Ru-yin HU ; De-hao FU
Chinese Medical Journal 2008;121(8):745-751
BACKGROUNDNanobone putty is an injectable and bioresorbable bone substitute. The neutral-pH putty resembles hard bone tissue, does not contain polymers or plasticizers, and is self-setting and nearly isothermic, properties which are helpful for the adhesion, proliferation, and function of bone cells. The aim of this study was to investigate the osteogenic potential of human bone morphogenetic protein 2 (hBMP2) gene activated nanobone putty in inducing ectopic bone formation, and the effects of the hBMP2 gene activated nanobone putty on repairing bone defects.
METHODSTwenty four Kunming mice were randomly divided into two groups. The nanobone putty + hBMP2 plasmid was injected into the right thigh muscle pouches of the mice (experiment side). The nanobone putty + blank plasmid or nanobone putty was injected into the left thigh muscle pouches of the group 1 (control side 1) or group 2 (control side 2), respectively. The effects of ectopic bone formation were evaluated by radiography, histology, and molecular biology analysis at 2 and 4 weeks after operation. Bilateral 15 mm radial defects were made in forty-eight rabbits. These rabbits were randomly divided into three groups: Group A, nanobone putty + hBMP2 plasmid; Group B, putty + blank plasmid; Group C, nanobone putty only. Six rabbits with left radial defects served as blank controls. The effect of bone repairing was evaluated by radiography, histology, molecular biology, and biomechanical analysis at 4, 8, and 12 weeks after operation.
RESULTSThe tissue from the experimental side of the mice expressed hBMP2. Obvious cartilage and island-distributed immature bone formation in implants of the experiment side were observed at 2 weeks after operation, and massive mature bone observed at 4 weeks. No bone formation was observed in the control side of the mice. The ALP activity in the experiment side of the mice was higher than that in the control side. The tissue of Group A rabbits expressed hBMP2 protein and higher ALP level. The new bone formation rate and antibending strength of group A was significantly higher than those of group B and C. The defects in blank control were not healed.
CONCLUSIONSThe hBMP2 gene activated nanobone putty exhibited osteoinductive ability, and had a better bone defect repair capability than that of nanobone putty only.
Absorbable Implants ; Animals ; Bone Morphogenetic Protein 2 ; Bone Morphogenetic Proteins ; analysis ; genetics ; Female ; Male ; Mice ; Osteogenesis ; physiology ; Rabbits ; Transforming Growth Factor beta ; analysis ; genetics