Objective To determine the difference of microvessel density(MVD) in each layer and region of abdominal flap. MethodsFlaps were obtained from 60 patients with abdominal operations,10 in each of the regions from I to VI.The vascular endothelial cells were marked with CD34 by immunohistochemistry,and the MVD in each region and layer of the abdominal flaps was determined.Results The vascular net of abdominal flap was divided into five layers: papillary layer of corium,papillary underlayer of corium,papillary deep layer of corium,superficial fascia and deep fascia.The mean MVD of the five layers were 17.80?1.68,9.12?1.84,27.91?2.44,37.18?6.55 and 46.91?7.02,respectively,with significant differences among them(P0.05).Conclusion Anatomic factors may be responsible for the survival of large abdominal flaps.Either of the direct and retrograde motion is feasible in the transplantation.

Bacteria ; genetics ; growth & development ; Biofilms ; Genetic Heterogeneity

Adolescent ; Adult ; Child ; Child, Preschool ; Female ; Graft vs Host Disease ; prevention & control ; Hematopoietic Stem Cell Transplantation ; methods ; Humans ; Male ; Middle Aged ; Nutritional Status ; Nutritional Support ; Young Adult

Therearemanywaystoestablishanimalmodelsofneonatalhyperbilirubinemia,suchasintraperitoneal or intravenous injection , genetic defect animal models , the use of chemical drugs , and injection of bilirubin into cerebellomedullary cistern , and so on . In order to study the etiology , pathogenesis , and therapy of neonatal hyperbilirubinemia through animal models , we review the literature on rodent and primate models of neonatal hyperbilirubinemia ,including establishment of models and their applications , in order to provide reference for the research of neonatal hyperbilirubinemia .

Along with the development of interventional appliances and proficiency of operational skills,the interventional diagnosis and treatment via hepatic portal vein system have achieved great progress and improvement.However,in order to further exploit the advantages of interventional diagnosis and treatment, the review of the anatomical structures,normal aberrance of portal venous system were needed.Getting familiar with pathologic condition to discover the new interventional appliances and embolic agents,and then in term of conduct the research on a very tough substancial base in a down-to-earth manner were important.

Objective To observe the expression of matrix metalloproteinase-9(MMP-9)/tissue inhibitor of metalloproteinase-1(TIMP-1) in tubulointerstitial fibrosis(TIF) rats and explore the effects of MMP-9 and TIMP-1 in TIF.Methods Forty-eight male Sprague-Dawley(SD) rats were randomly divided into sham-operated group(n=24) and model group(n=24).Results Tubulointerstitial fibrosis model was established via unilateral ureteral obstruction(UUO). Histological changes were observed in tubulointerstitium injury under microscope and immunohistochemistry staining was performed to investigate both the expression of MMP-9 and TIMP-1 in TIF by semiquantitative score at d7, d14, d21 after experiment onset.The expressions of TIMP-1 in rats from sham-operated group were lower than those of mo-del group rats at d7, d14, d21 (Pa

Objective To investigate the changes of SCD 14, TNF-?, E-SLT and IL-10 level in the process of infection.Methods Serum E-SLT, IL-10, SCD 14 and TNF-? level was measured in 37 patients of abdominal trauma, and in model rabbits with endotoxemia.Results Serum level of SCD 14,TNF-?,E-SLT and IL-10 on the 1st to 3rd day post-op increased significantly in patients suffering from post-op infection 〔(1.61?0.47)??g/ml, (28.63?8.29)?pg/ml,(153.6?48.9)?ng/ml and (38.21?10.87)?pg/ml, compared with control, all P

0.05 ), and the effect in Group A and Group B was superior to Group C(P

[Objective] To investigate the therapeutic effect of early anterior bone grafting and internal fixation after focal cleaning and vertebral canal decompression for the treatment of tuberculosis of thoracolumbar vertebrae. [ Methods ] A retrospective study was carried out in 26 patients with thoracolumbar vertebral tuberculosis (TVT) who received early anterior bone grafting and internal fixation after focal cleaning and vertebral canal decompression. After treatment, the therapeutic effect was evaluated by observing the recovery of nerve function. [Results] The result of a follow-up ranging from 8 months to five years and 4 months showed that all the cases wi vertebral tuberculosis were cured, free from relapse. The internal fixation and bone graf(?)ing for fusion were good, and nerve function recovered, maintaining a good state. The nerve function of six patients with incomplete paralysis fully recovered. [Conclusion] Early anterior bone grafting and internal fixation after focal cleaning and vertebral canal decompression are effective to promote the recovery of nerve function of TVT patients. This regimen is capable of relieving or preventing spinal compression promptly, rebuilding the stability of the spine and presenting the focal diffusion, beneficial to the recovery of the patients.