Analysis samples of meat and seafood... in Hanoi markets to determine infections of larva of parasites. Results: among 144,390 beef samples and 2,237,000 pork samples, prevalences of parasite infection were 0.027% and 0.003%, respectively. The rate of liver fluke metacercaria in fresh water fish was 1.7-21.7% (mean rate 5.2%), rate of Gnathostoma infection in snakehead fish and ell was 4.8-11.4%. Furthermore, in endemic areas of lung fluke, infection rate of Paragonimus metacercaria in fresh water crabs and fish was 9.7%-98.1% (mean rate 63%).
Food Contamination ; Parasites ; Food ; Epidemiology

A survey concerning the infestation of clonorchis sinensis in human was conducted in 4 communes of Ninh Binh and Nam Dinh provinces, from each, 100 households with their members of above 6 years old of age were enrolled randomly. Results showed a prevalence of 31.3 – 37.3% in Nam Dinh and 22.5% in Ninh Binh epidemic areas. The prevalence in male subjects was higher than in female (45.5 –47.6% versus 10.9 – 26.1%). The age group of 40-49 years old had had the highest prevalence (51.9%). There, 35.9 – 80.4% of people had consumed raw fish.
Opisthorchis ; Epidemiology ; Clonorchis sinensis

Angiostrongylosis is roundworm disease caused by Angiostrongylus. Angiostrongylus including 20 species parasitize mainly in mouse, among which two species cause disease in human are Angiostrongylus cantonensis and Angiostrongylus costaricensis. Angiostrongylus cantonensis was found by Chen H.T in 1935 in China. For pediatric case records that having meningitis syndrome with symptoms of fever, headache, paralytic, cerebrospinal fluid clear or unclear but eosinophil increasing highly, it needs to think of meningitis caused by parasites and do immune test to Angiostrongylus to identify the cause. Early treatment will have good results and avoid severe consequences
Meningoencephalitis ; Angiostrongylus

b cytochrome oxydase gene fragment of the mitochondric geneme includes 652 nucleotides and aminoacide of taenia solium isolated from Vietnamese [symbolized as TsoN (VN)] was received by PCR reaction, purified, cloning and expressed in sequence. Results were compared with those of corresponding sequence of some genera of Taenia solium in China (T. saginata) in Taiwan (Taeniaasiatica) and in Viet Nam. Cysticercoid larve of Taenia solium isolated from Vietnamese was similar with that isolated from pig in Viet Nam and China, but there are high difference between the genera T. saginata (in China) and T. asiatica (in Taiwan and Viet Nam). The analysis determinated various varieties of eysticercoid larve as Taenia sodium’s larva. This is the first determination of this genus in Viet Nam
Taenia solium ; Cysticercus ; Patients

Using polymerase chain reaction (PCR), a portion of the cytochrome oxidase 1 (446 nucleotide and 148 amino acid) of mitochondrial genome of adult worm of small liver fluke from human in Hiep Hoa district, Bac Giang (CsNgBG) province was amplified. The nucleotide and amino acid of these sequences were comparatively analyzed with the known Clonorchis sinensis. The analysis revealed that the Bac Giang Clonorchis sp (in human) was similar (100% nucleotide and amino acid) to the Nam Dinh Clonorchis sinensis (identification was done); 99.6% (nucleotide) and 100% (amino acid) homology to the C.sinensis of Chinese strain (CsCN-Gen Bank AF 184619) and Korean strain (CsKor-Gen Bank AF 181889). Thus, the Bac Giang Clonorchis sp is identified as Clonorchis sinensis in Opisthorchidae family, Clonorchis genus. Phylogenetic analysis uniquely placed the Bac Giang Clonorchis sinensis to the group of Chinese and Korean Clonorchis sinensis.
Helminths ; Fasciola hepatica ; Adult

The survival ability of the larvae of Clonorchis sinensis in raw fish dish prepared by traditional culinary method in Nghia Lac commune, Nghia Hung District, Nam Dinh province and the larvae of Paragonimus heterotrimus in grilled crabs processed in Khanh Hoa commune, Luc Yen district, Yen Bai province was investigated. In this raw fish dish, 93-95% of the microcerca of Clonorchis sinensis and in this grilled crab, 23.3-65% of microcerca had been living
Clonorchis sinensis ; Fishes ; Paragonimus

Fasciolopsis buski is common parasite in pigs. Some patients who infected by adult worms of Fasciolopsis buski in several areas, including the provinces of Ninh Binh and Nghe An were sampled. Genome of 18S ribosomal RNA with 1950 nucleotides from giant intestinal fluke (Fasciolopsis buski) was obtained using polymerase chain reaction (PCR) and sequencing approach for comparative analysis each others and with the data deposited in GenBank (L06668). The adult worms from Ninh Binh and Nghe An were morphologically identified as Fasciolopsis buski. The genetic analysis revealed that the 18S rRNA gene of the giant intestinal flukes isolated in Ninh Binh and Nghe An were similar (homogenous 100% of nucleotides) and showed nearly absolute identity with only 2 divergent nucleotides of 0.01% (2/1950 nucleotides) to the 18S rRNA of the pig Fasciolopsis buski as the data deposited in GenBank (L066608). However, 18S ribosomal RNA of the adult worms of human Fasciolopsis buski collected from the provinces of Ninh Binh and Nghe An were similar to one another
Fasciolidae ; RNA

Triclabendazole was used in treatment of 249 fascioliasis patients selected from 25 provinces including 19 Northern and 6 Southern ones. Two different doses were used for two groups: 10mg/kg/body for 226 patients, and 20mg/kg/body for 43 others, twice per day with the interval 6-8 hours from meals. Symptoms on these fascioliasis patients were found as positive ELISA test with Fasciola gigantica antigen (100%), prejudice in liver by ultrasound (87.9%), pain of liver (87.1 %), eosinophilia (63.5%), plodding (26.1 %), fever (39.8%), digestive disorder (20.1%) and positive stool examination with Fasciola egg (16.9%). Most of symptoms were decreased and disappeared within 1 month after treatment except for pain of liver in some patients that lasted longer and disappeared within 6 to 12 months after treatment. The cure rate was 92.9% for 1 month after treatment, 95.2% for 3 months after treatment and 100% for 6 months after treatment. Ultrasound prejudice in liver decreased and disappeared 80.9% for 1 month, 92.6% for 3 months, 96.3% for 6 months and 100% for 12 months after treatment. Eosinophilia rate returned to normal of 90.7% for 6 months and 100% for 12 months after treatment. ELISA test with F. gigantica antigen become negative of 89.4% for 6 months and 100% for 12 months. GOT, GPT, urea and creatinin tests had not pathological change by 1 month using triclabendazole. Side effect of triclabendazole was inconsiderable and disappeared without medical treatment. Triclabendazole may be recommended in treatment for fascioliasis in Vietnam with doses of 10 or 20 mg/kg of body weight.
Fasciolidae ; Therapeutics ; Benzimidazoles

A region of mitochondrial cytochrome oxidase 1 gene (coxl) for Clonorchis sp samples from the provinces of Thanh Hoa and Nam Dinh, and Opisthorchis sp from human in Phu Yen province, Vietnam was analyzed by PCR. Nucleotide sequence of these coxl fragments was used to compare with Opisthorchis viverrini, strain Khon Ken (Thailand) and Clonorchis sinensis sequences originated from China and Korea. The results revealed that nucleotide sequence of Opisthorchis sp from Vietnam had absolute homology to O. viverrini (Thailand) but different from C. sinensis (from China and Korea). Clonorchis sp from Vietnam was similar with Clonorchis sinensis from China and Korea. Opisthorchis sp isolated in Phu Yen is, thus, molecularly indentified as Opsithorchis viverrini and Clonochis sp in Thanh Hoa and Nam Dinh is Clonorchis sinensis. Two species are different in morphology and molecular level.
Mitochondria ; Clonorchis sinensis

Since 2002, adult worms of human small liver fluke in some provinces were identified by morphology and confirmed by molecular method as Clonorchis sinensis in Thanh Hoa, Ninh Binh, Nam Dinh and Bac Giang province; Opisthorchis viverrini in Phu Yen and Binh Dinh province. This study, using polymerase chain reaction (PCR), a portion of the cytochrome oxidase 1 (410 nucleotide and 136 amino acid) of mitochondrial genome of adult worms of small liver fluke from human in Nghe An (CsNA) and Dong Nai (CsDN) province were amplified. The nucleotide and amino acid of these sequences was comparatively analyzed with the known Clonorchis sinensis (Nam Dinh strain). The analysis revealed that the Nghe An Clonorchis sp and Dong Nai Clonorchis sp (in human) were homology (99.5 - 99.8% nucleotide) and similar (100% amino acid) to the Nam Dinh Clonorchis sinensis (identification was done). Thus, the Nghe An and Dong Nai Clonorchis sp were identified as Clonorchis sinensis in Opisthorchiidae family, Clonorchis genus.
Helminths ; Trematoda ; Human