1.Significance of Expression of Interferon ? Inducible Protein 10 in Peripheral Blood Mononuclear Cells in Children with Kawasaki Disease
de-sheng, JIA ; ji-min, SUN ; zong-qi, DONG
Journal of Applied Clinical Pediatrics 2006;0(19):-
0.05).Conclusions The expression of PBMC IP-10 mRNA increases in KD.IP-10 may participate in the pathogenesis of KD and CAL in KD.Perhaps the inhibition to the expression of PBMC IP-10 mRNA is one of the mechanisms of IVIG.
2.Contact heat evoked potential:a method of detection
Ju-Yang ZHENG ; Ying-Sheng XU ; Shuo ZHANG ; Jun ZHANG ; De-Xuan KANG ; Dong-Sheng FAN
Chinese Journal of Neurology 2001;0(02):-
Objective To evoke cerebral potentials by stimulating nociceptive fibers with contact heat evoked potentials stimulator (CHEPS)and estimate the nerve conduction velocities of peripheral nerve fibers mediating these responses.Methods Subjects were set in supine position.A heat-foil technology with a rapid rising speed at 70 ℃/s was used to elicit pain and contact heat evoked potentials(CHEP).Contact heat was delivered via one circular thermode (diameter 27 mm,area 573 mm~2).Thermal stimuli were sent at two intensity levels (49.5 ℃ and 54.5 ℃) to three body sites:thenar eminence,the dorsum of hand and proximal volar forarm.Contact heat evoked potentials were recorded from Cz and Pz.A systemic effect between stimulus intensities and pain rating were observed,the main components of this evoked potential were observed.Nerve conduction velocity was calculated from latency difference of CHEP and center to center distance of distal and proximal stimulus arrays.Results The pain intensity rating was 3.2?0.3 and 4.4?0.5 when thenar eminence was stimulated at the temperature of 49.5 ℃ and 54.5 ℃ respectively;the rating was 6.3?0.8 and 7.2?0.5 when the dorsum of hand and proximal volar forarm were stimulated at the temperature of 54.5 ℃ respectively.Three components,Cz/N550,Cz/P750 and Pz/P1000,were found in the evoked potentials.Nerve conduction velocities of the fibers were (12.9?7.5) and (1.7?0.4) m/s respectively,which were corresponding to those of A8 fiber and C fiber.Conclusions CHEPs can be elicited reliably and stably.Velocities of peripheral nerve fibers demonstrate that A8 fiber and C fiber mediate the response.
3.Abnormal trigeminocervical response in patients with spinal and bulbar muscular atrophy
Ming Lu ; Ying-Sheng Xu ; Ju-Yang Zheng ; Shuo Zhang ; De-Xuan Kang ; Dong-Sheng Fan
Neurology Asia 2012;17(3):209-212
Objective: To investigate the value of the trigeminocervical response (TCR) for revealing bulbar
involvement in patients with spinal and bulbar muscular atrophy (SBMA). Methods: Thirty patients
with SBMA and 30 healthy male controls were included in this study. In all of the normal controls,
stimulation of the infraorbital nerve on one side produced bilateral short latency waves consisting
of a positive/negative wave, p19/n31, the mean latency of which was measured. The mean square
root of the ratio between the amplitude of p19/n31 and the mean rectifi ed surface electromyography
(EMG) activity preceding the stimulus, the A value, was estimated. The parameters of the TCR
were compared between the two groups. Results: Among the patients with SBMA, 21 (70.0%) had
delayed latencies of p19/n31 (P < 0.01) and all (100%) had reduced A values (P < 0.01) relative to
the normal controls.
Conclusions: All parameters of the TCR were signifi cantly different between the patients with SBMA
and the normal controls. T
4.Contact heat evoked potential:the method,normative reference data and it's application in cerebral infarction
Ying-Sheng XU ; Ju-Yang ZHENG ; Shuo ZHANG ; Jun ZHANG ; De-Xuan KANG ; Dong-Sheng FAN ;
Chinese Journal of Physical Medicine and Rehabilitation 2003;0(09):-
Objective To establish the method of contact heat evoked potential(CHEP)and to explore the value of this evoked potential in pain testing of patients with cerebral infarction.Methods A total of 100 healthy volunteers and 30 patients were examined.The healthy volunteers were divided into 3 groups according to the length of their arms:(Group A:56.0~65.0 cm ;Group B :65.5~74.0 cm ;Group C :74.5~83.0 cm).A recently de- veloped heat-foil technique with a rapid temperature rising rate at 70℃/s was used to elicit pain and contact heat e- voked potentials.Contact heat was delivered via one circular thermode(diameter 27 mm,area 573 mm~2)and set at two intensity levels(49.5℃and 54.5℃)to three body sites:the thenar eminence,the dorsum of hand and proximal volar forearm.The subjects were asked to rate the pain with numerical rating scale after each stimulus and CHEP was recorded from Cz and Pz.The association between stimulus intensities and pain rating was explored,the main compo- nents of the evuked potential were watched.CHEP,sensory conduction velocity(SCV)and somatosensory evoked potentials(SEP)were performed in patients with hemi-anesthesia caused by cerebral infarction.Results The pain intensity ratings were 3.2?0.3 and 4.4?0.5 at thenar eminence,5.0?0.7 and 6.3?0.8 at the dorsum of hand and 5.3?0.6 and 7.2?0.5 at the proximal volar forearm when the temperature of 49.5℃and 54.5℃was applied, respectively;Three components,Cz/N550,Cz/P750 and Pz/P1000,were identified in the evoked potentials.Cz/ N550 and Cz/P750 appeared when the dorsum of hand and proximal volar forearm were stimulated.In contrast,Pz/ P1000 could be identified when nociceptors of thenar eminence and proximal volar fbrearm were excited.In the pa- tients with cerebral infarction,CHEP disappeared or became abnormal on one side,while SCV and SEP were normal on that side.Conclusion It was suggested that CHEP could be elicited reliably in the controls.CHEP is helpful in the assessment of analgesia in patients with cerebral infarction.
5.Hemorrhage after duodenopancreatectomy
Cai-De LU ; Dan WU ; Feng QIU ; Zhi-Long YAN ; Han-Ting LING ; Ying-Chun SHENG ; Sheng-Dong WU
Chinese Journal of General Surgery 2000;0(12):-
Objective To study the sources and the relationship between the management and the outcome of hemorrhage after cephalic pancreatoduodenectomy.Methods The clinical data of 370 patients who underwent pancreatic resection at the Lihuili Hospital and the Second Affiliated Hospital of Zhejiang University were retrospectively analyzed.Results Postoperative bleeding occurred in 35 patients with 11 deaths.Among those intraabominal bleeding occurred in 14 cases and gastrointestinal hemorrhage occurred in 22,with one case suffering from both.Bleediug developing within 72 hours after operation in 12 cases (early-stage group),which was caused by improper intraoperative homeostasis.In other 23 cases,bleeding 72 hours after operation(later stage group)was caused by the erosion following pancreatic and/or bile leakage.Relaparotomy was performed in 13 cases and endoscopic homeostasis was performed in 3. Relaparotomy or endoscopic homeostasis was superior to that of conservative therapy in the early-stage group (P0.05).Pancreatic or bile leakage was identified as the significant risk factors for the postoperative bleeding.Conclusions In order to prevent the postoperative hemorrhage and to reduce the mortality of pancreatic resection,skillful techniques,expeditious homeostasis,proper management of stump pancreas and the prevention of pancreatic and bile leakage are essential.
6.MRI diagnosis of Hirayama disease
Yu FU ; Dong-Sheng FAN ; Xin-Long PEI ; Hong-Bin HAN ; Jun ZHANG ; De-Xuan KANG ;
Chinese Journal of Radiology 2001;0(04):-
Objective To investigate the dynamic changes of the spinal cord during neck flexion in Hirayama disease for diagnosis.Methods MRI examinations in neutral neck position and a fully flexed neck position were performed on 18 cases of Hirayama disease and 31 young normal control subjects.We measured an antero-posterior diameter(APD)and transverse diameter(TD)of the cervical cord at the superior margin of the C6 vertebral body for each position,and investigate the dynamic changes.The different in frequency of these findings between the control and patient groups was examined by means of the x~2 test.The group means were compared by independent-sample t-test.Significance was defined as P
7.The change of immunoreactivity in glia cells and its sense by using early Parkinson's disease rat model.
Hong XU ; Fang-Yuan DONG ; Sheng-Ming YIN ; Dong-Mei WANG ; Yi-Ping SUN ; De-Qin YU ; Wan-Qin ZHANG
Chinese Journal of Applied Physiology 2012;28(1):71-93
Animals
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Astrocytes
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immunology
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metabolism
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Male
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Microglia
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immunology
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metabolism
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Neurons
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metabolism
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Oxidopamine
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metabolism
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Parkinson Disease
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immunology
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Rats
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Rats, Sprague-Dawley
8.The effect of shourong compound formula on levels of dopamine and its metabolites in brain of Parkinson's disease mice induced by reserpine.
Wei WANG ; De-zhong ZHAO ; Xiao-fang SUN ; Sheng-dong GU
China Journal of Chinese Materia Medica 2002;27(5):368-370
OBJECTIVETo investigate the effect of Shourong compound formula on treating Parkinson's disease.
METHODParkinson's disease model mice induced by reserpine was used and by HPLC-ED the levels of Dopamine (DA) and its metabolites were determined.
RESULTMadopar could increase the levels of DA in brain of PD mice. The effect of madopar together with Sourong compound formula was better than that of madopar(P < 0.001).
CONCLUSIONShourong compound formula together with madopar has synergic effect on increase of DA level in brain and can reduce clinic dose of madopar so that side effect of madopar can be decreased.
3,4-Dihydroxyphenylacetic Acid ; metabolism ; Animals ; Benserazide ; pharmacology ; Brain ; metabolism ; Dopamine ; metabolism ; Drug Combinations ; Drug Synergism ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; Homovanillic Acid ; metabolism ; Levodopa ; pharmacology ; Male ; Mice ; Parkinson Disease, Secondary ; chemically induced ; metabolism ; Plant Extracts ; pharmacology ; Plants, Medicinal ; chemistry ; Reserpine
9.Dynamic changes of 5-HT, DA and their metabolin in rat striatum during exhaustive exercise and recovery.
Dong-Sheng YANG ; Xiao-Li LIU ; De-Cai QIAO
Chinese Journal of Applied Physiology 2011;27(4):432-436
OBJECTIVETo study the dynamic characteristics of serotonin (5-HT), dopamine (DA) and their metabolin changes in brain during the development of exercise-induced central fatigue.
METHODSCoupling of microdialysis and capillary electrophoresis-laser induced fluorescence detection method were used to continuously monitored the changes of DA, tryptophan (Trp), tyrosine (Tyr), 5-HT and 5-hydroxyindoleacetic acid (5-HIAA) in striatum extracellular fluid during the exhaustive exercise and recovery time.
RESULTSThe concentrations of Trp, 5-HT, 5-HIAA in striatum extracellular fluid had no remarkable changes in the early time of exercise (P < 0.05), while they significantly increased during the later time of exercise and whole recovery time (P < 0.05, P < 0.01). The concentrations of DA and Tyr significantly increased over basal level in the later exercise time, exhaust and recovery time (P < 0.05, P < 0.01). DA/5-HT significantly increased in the initial time of exercise (P < 0.05, P < 0.01), while decreased during the later exercise time, the nadir occurred at 15 minutes before rats exhausted. DA/5-HT slightly recovered back to basal level during the recovery time, and there was no significant difference during later exercise, exhausted and recovery time compared with basal level (P < 0.05).
CONCLUSIONThe changes of DA and 5-HT in striatum have phase characteristics. Both of them significantly increase during the development of exercise-induced fatigue. However, the 5-HT plays the dominant role in the dynamic changes of them.
Animals ; Corpus Striatum ; metabolism ; Dopamine ; metabolism ; Fatigue ; metabolism ; physiopathology ; Male ; Physical Conditioning, Animal ; physiology ; Physical Exertion ; physiology ; Rats ; Rats, Wistar ; Serotonin ; metabolism
10.Establishment of human lung squamous carcinoma cell line CHLH-1
Hong-Cheng LIU ; Sheng-Dong HUANG ; De-Jun GONG ; Xiao-Hong LIU ; Yang YUAN ; Zhi-Yun XU ;
Academic Journal of Second Military Medical University 1999;0(12):-
Objective:To establish a human lung squamous carcinoma cell line and to study its biological characteristics. Methods:Lung squamous carcinoma specimens were freshly resected during operation;the tissues were incubated in vitro and the cell line was named CHLH-1.The biological characteristics of the cells were studied by light microscopy,electron microsco- py,chromosome analysis and transplantation experiment.Results:Cells from the specimens of the primary tumor,the CHLC- 1 cell line and the cells from transplanted tumor possessed the characteristics of malignant squamous epithelium under light and electron microscope.The cell growth curve,doubling time and mitotic index were also observed in vitro.Nuclear chromosome analysis revealed that the tumor was a subtriploid with a mode of 60-68 per cell.Tumor nodes were observed under the skin of nude mice by heterogenic transplantation.Conclusion:The characteristics of the established cell line suggest that it is a newly established human squamous carcinoma cell line.