2.Multiplex RT-PCR assay for the detection of 8 kinds of respiratory viruses
De WU ; Yanling MO ; Lirong ZOU ; Hui LI ; Qiuxia CHEN ; Ling FANG ; Ping HUANG ; Changwen KE
Chinese Journal of Laboratory Medicine 2009;32(8):861-865
. Conclusions Two multiplex RT-PCR assays show high consistency with common RT-PCR. The multiplex RT-PCR assays were initially established.
3.Clinical analysis of 126 children with hemorrhagic fever with renal syndrome.
Cui-ping WU ; Ru-yong LI ; Liang-yi QI ; De-quan XIA
Chinese Journal of Pediatrics 2003;41(9):703-704
Adolescent
;
Child
;
Child, Preschool
;
Electrocardiography
;
Female
;
Fever
;
complications
;
Hemorrhagic Fever with Renal Syndrome
;
blood
;
complications
;
pathology
;
Humans
;
Hypergammaglobulinemia
;
blood
;
Immunoglobulin M
;
blood
;
Male
;
Pain
;
complications
4.Intravenous drug abuse-related infective endocarditis: report of an autopsy case.
Wei-xiang ZHONG ; Dong-ping TIAN ; De-qing WU ; Min SU
Chinese Journal of Pathology 2010;39(6):421-422
Adult
;
Aortic Valve
;
microbiology
;
pathology
;
Autopsy
;
Brain
;
microbiology
;
pathology
;
Endocarditis, Bacterial
;
complications
;
microbiology
;
pathology
;
Female
;
Heart Ventricles
;
microbiology
;
pathology
;
Humans
;
Mitral Valve
;
pathology
;
Sepsis
;
complications
;
microbiology
;
pathology
;
Substance Abuse, Intravenous
;
complications
;
microbiology
;
pathology
;
Young Adult
5.Rapidly detect and distinguish between norovirus G I and G II type with a pair of primers.
Jian-Kang HAN ; Xiao-Fang WU ; De-Shun XU ; Li-Ping CHEN ; Lei JU
Chinese Journal of Experimental and Clinical Virology 2013;27(5):379-381
OBJECTIVEThe purpose of this study was to develop RT- PCR assay for Rapidly detect and distinguish between Norovirus genogroup I and genogroup II with a pair of primers.
METHODSA pairs of primers specific to capsid prote in ORF2 gene of G I and G II Norovirus were dsigned according to the published complete genome sequence, with which the RNA of Norovirus was extracted and RT-PCR amplification. The sensitivity, specificity of the RT- PCR assay was estimated and apply it to the detection of Norovirus in clinical specimens.
RESULTSThe results showed that the assay possessed high specificity for Norovirus detection and without any evident cross-reaction with other viruses, including rotavirus, enteric adenovirus and hepatitis A virus. The detection limit of RT-PCR assay for Norovirus G I and G II were up to 100 pg/ml and 10 pg/ml respectively.
CONCLUSIONThe RT- PCR assay provide rapid and sensitive detection of Norovirus G I and G II and should prove to be useful for Norovirus diagnosis in the outbreaks of acute gastroenteritis.
Caliciviridae Infections ; diagnosis ; virology ; DNA Primers ; genetics ; Gastroenteritis ; diagnosis ; virology ; Humans ; Norovirus ; classification ; genetics ; isolation & purification ; Reverse Transcriptase Polymerase Chain Reaction ; instrumentation ; methods
6.Time-dependent changes of astrocytes and CD31-marked microvessels in human cerebral infarction
De-Sheng WANG ; Ji-Ping QI ; Guang-Wei LI ; He WU ; Dan-Dan WANG ;
Chinese Journal of Geriatrics 2001;0(05):-
Objective To observe the time-dependent changes of astrocytes and microvessels in the ischemic core and surrounding areas.Methods Glial fibrillary acidic protein (GFAP)was measured by HE,and CD31 by immunohistochemistry as markers in post-mortem specimens from ten patients,who died of cerebral infarction.Results Each section of the infarcted brains was divided into four areas (the area 0-3).GFAP expressed a little in area 0 and 1,increased in a time-dependent manner in area 2 and 3;CD31 die not expressed in area 0,expressed a little in the area 1,and increased in area 2 and 3 continuously.Conclusions The proliferation of astrocytes and microvessels may play a significant role in the process of restoration after cerebral infarction.
7.Research progress of growth factors in the pathogenesis and developments of myopia
De-Long, SHI ; Jian-Feng, WU ; Guo-Ping, LI ; Hong-Sheng, BI
International Eye Science 2016;16(7):1273-1275
Myopia is one of the most popular eye diseases all over the world. The development of the current understanding of its mechanism is still limited. Many studies indicated that the growth factors closely related to eye development and myopia. Some growth factors with biological activity, such as transforming growth factor ( TGF ), fibroblast growth factor ( FGF ) and epidermal growth factor ( EGF ), have an impact on scleral thickness variation, the regulation of the development of myopia and so on, which plays a non-negligible role in the pathogenesis of myopia. In this paper, the function of various growth factors in myopia will be reviewed.
8.Purification of Recombinant Fusion Protein Staphylokinase-Hirudin Expressed by Escherichia coli and Analysis of its Dimer
Gen-Shen ZHONG ; Ai-Ping YU ; Ji-De JIN ; Zhong-Hua JIANG ; Zu-Ze WU ;
China Biotechnology 2006;0(02):-
The recombinant fusion protein staphylokinase-hirudin(rSFH) was purified from the high density-fermented engineered E.coli by means of ion-exchange chromatography (IEC) and gel filtration (GF). The purity of rSFH reached to more than 98% determined by RP-HPLC and SDS-PAGE, and the yield was up to 0.7g per liter of fermentation broth. The analysis of homologous dimmer of rSFH appeared during the purification and calculation of the surface hydrophobic area had been carried out by means of hydrophobic chromatography and MALD-TOF. The influence of sodium chloride and temperature on the behavior of rSFH reversible dimerization was analyzed by high performance sized- exclusive chromatography(HPSEC). It is concluded that the hydrophobic interaction played an important role in the reversible dimerization of rSFH.
9.Seroprevalence and diagnostic value of aquaporin-4 antibody in patients with inflammatory central nervous system demyelinating diseases.
Lei WU ; Yang YANG ; De-Hui HUANG ; Wei-Ping WU
Journal of Southern Medical University 2011;31(2):350-352
OBJECTIVETo assess the seroprevalence and diagnostic value of aquaporin-4 antibody (AQP4-Ab) in patients with inflammatory central nervous system demyelinating diseases.
METHODSSeventy-two patients with neuromyelitis optica (NMO), 68 with multiple sclerosis (MS), 4 with optic neuritis (ON), and 41 with transverse myelitis (TM) were included in this study. The TM group comprised 19 patients with non-longitudinally extensive transverse myelitis (nLETM), 14 with monophasic longitudinally extensive transverse myelitis (mLETM), and 8 with recurrent longitudinally extensive transverse myelitis (rLETM). The serum levels of AQP4-Ab was detected by indirect immunofluorence assay in these patients.
RESULTSAQP4-Ab was detected in 72.2% (52/72) patients with NMO, 5.9% (4/68) patients with MS, 25.0% (1/4) patients with ON, and 17.1% (7/41) patients with TM, showing a significant difference in the positivity between NMO and MS groups (P<0.01). AQP4-Ab seropositivity rate was 5.3% (1/19) in nLETM patients, 62.5% (5/8) in rLETM patients and 7.1% (1/14) in mLETM patients, significantly higher in rLETM than in nLETM (P<0.01) and mLETM groups (P<0.05), but no statistical difference was found between rLETM and NMO groups.
CONCLUSIONSA high seroprevalence of AQP4-Ab is observed in patients with NMO and rLETM, which support the hypothesis that NMO and rLETM belong to NMO spectrum disorders. AQP4-Ab can serve as a useful index for diagnosing NMO and differential diagnosis from MS. More attention and effective immunosuppressive treatments should be given to patients positive for AQP4-Ab.
Aquaporin 4 ; immunology ; Autoantibodies ; blood ; Demyelinating Autoimmune Diseases, CNS ; diagnosis ; immunology ; Female ; Humans ; Male ; Multiple Sclerosis ; diagnosis ; immunology ; Neuromyelitis Optica ; diagnosis ; immunology ; Seroepidemiologic Studies
10.Magnetic resonance imaging characteristics of spinal cord lesions and brain abnormalities in Chinese patients with neuromyelitis optica.
Lei WU ; Yang YANG ; De-hui HUANG ; Hui XIAO ; Wei-ping WU
Journal of Southern Medical University 2010;30(12):2733-2737
OBJECTIVETo analyze the magnetic resonance imaging (MRI) characteristics of neuromyelitis optica (NMO) in Chinese patients.
METHODSWe retrospectively reviewed the MRI films of 61 patients with NMO (including 57 female and 4 male patients) admitted in our department.
RESULTSOf these patients, 39 (79.6%) showed positivity for serum aquaporin-4 (AQP4) antibody. On MRI, 18 patients showed involvement of the cervical cord alone, 27 had both cervical and thoracic segment involvement, and 16 displayed thoracic segment involvement. The lesions appeared linear (9 cases), diffuse (23 cases), or both (29 cases), mostly located axially with occasional lateral distribution. Thirty-nine of the 61 patients (63.9%) had brain abnormalities, 31 presented with supratentorial lesions (mostly in the juxtacortical, subcortical, deep white matter and lateral ventricle-adjacent regions, n=27), 15 showed infratentorial lesions (mostly in the preiaqueduct-fourth ventricular-central canal, n=13), and 7 had supra- and infratentorial lesions simultaneously.
CONCLUSIONNMO has complex MRI presentation, and linear lesions in the spinal cord and preiaqueduct-fourth ventricular-central canal lesions, where AQP4 is high expressed, can be characteristic for NMO. MRI and AQP4 antibody detection are suggested for suspected cases for early diagnosis.
Adult ; Aquaporin 4 ; immunology ; Brain ; pathology ; Female ; Humans ; Magnetic Resonance Imaging ; Male ; Neuromyelitis Optica ; pathology ; Retrospective Studies ; Spinal Cord ; pathology