Objective: To investigate the effect of four kinds of inactivation methods on the activity of soybean agglutinin (SBA). Methods: Dry-heat, humid-heat, acid-alkali and metal ion were employed to evaluate their effects on the activity of SBA, which were quantified by hemagglutination. Results: Dry-heat below 120℃ did not obviously decrease the activity of SBA, while humid-heat (95℃ for 30 min,100℃ for 20 min or 105℃ for 10 min) completely inactivated the SBA. The activity of SBA significantly decreased when soybean protein was extracted under pH 1.0-4.5 or pH 9-13. Quadrivalent and trivalent metal ions markedly inhibited the activity of SBA. Conclusion: SBA can be inactivated effectively by humid-heat, acid-alkali or metal ion. Further study is needed to clarify whether there are synergistic effects between these treatments.

OBJECTIVE:To establish the gas chromatography (GC) fingerprint for the petroleum ether part of Areca peel and provide reference for the quality evaluation. METHODS:GC was conducted to establish the fingerprint for the petroleum ether part of Areca peel from 10 different areas,the cluster analysis and similarity of fingerprint data were conducted to study the similarity of GC fingerprint of Areca peel. RESULTS:There were totally 8 common peaks. All the medicine materials had the characteristics,but there were differences among the relative peak area. CONCLUSIONS:The GC fingerprint has high importance and precision and can be used for the quality control of Areca peel.