Objective:To investigate the protective effects and mechanism of ligustrazine on myocardial ischemia reperfusion injury(MIRI) in rats.Methods:30 Wistar rats were divided into three groups randomly:sham operation group,ischemia reperfusion injury group(IR),and ligustrazine preconditioning group(LI).The model of MIRI was produced,Immunohistochemical S-P method was used to detect the expressions of HSP25 and p38MAPK proteins in MIRI tissues.Results:Compared with IR group,the expression of HSP25 protein in LI group increased significantly(P

BACKGROUNDSurgical resection becomes standard treatment for pulmonary metastatic tumor with operative indication. The aim of this study is to analyze the indication and surgical efficacy of pulmonary metastasectomy.

METHODSFrom November 1991 to May 2003, a total of 26 patients with pulmonary metastatic tumors were treated surgically in our hospital, 12 cases were males and 14 females, with an average age of 50 years old. The metastatic tumors came from different organs and tissues, 5 cases accompanied by metastatic tumors of liver or brain.

RESULTSFour cases underwent pneumonectomy, 8 lobectomy, and 14 wedge resection (in which 8 cases underwent mildly invasive operation using video-assisted thoracic surgery). No operative death occurred. 5-year survival rate was 27.3% (3/11), 2-year survival rate 42.9% (9/21) and 1-year survival rate 69.2% (18/26).

CONCLUSIONSPulmonary metastasectomy is recommended in pulmonary metastases with operative indication, and is still effective in cases accompanied by resectable metastatic tumors of liver or brain. Mildly invasive operation using video-assisted thoracic surgery might be chosen for some cases with single metastatic locus.

【Objective】 To retrospectively investigate the antibody distribution and pregnancy outcome of pregnant women with Rh alloantibody in Guangzhou, and summarize the prevalence, diagnosis and treatment experience of hemolytic disease of newborn (HDN) caused by Rh alloantibody, so as to provide data for the prevention, diagnosis and treatment of Rh-HDN. 【Methods】 A total of 17 345 pregnant women in Guangzhou from January 2014 to December 2020 were selected for irregular antibody screening test.Those with Rh antibody were followed up for delivery, and the clinical and laboratory examination results of pregnant women and newborns were analyzed. 【Results】 A total of 71 cases (0.41%, 17/17 345) with Rh alloantibodies were detected.Among them, anti-D, anti-E, anti-Ec, anti-C, anti-Ce, anti-c, anti-e accounted for 26.76% (19/71), 46.48% (33/71), 9.86% (7/71), 7.04% (5/71), 5.63% (4/71), 2.82% (2/71) and 1.41% (1/71), respectively.Among the 71 pregnant women, 34 gave birth to children with HDN, with the total prevalence rate of 47.89%, among whom 100%, 78.94% and 42.42% were anti-c, anti-D and anti-E, respectively.And 71.43% (5/7) of the children who underwent transfusion were with anti-D.Although the yield rate of anti-E was the highest, it involved low morbidity and mild symptoms, which preferred to occur in the first fetus.No significant difference in gestational age, birth weight and the occurrence time of jaundice was notice between the anti-D group and anti-E group, but the total bilirubin of the anti-E group was lower while the Hb level were higher than those of the anti-D group (P<0.05). Two children died, and others were cured by phototherapy, albumin, IVIG and blood transfusion. 【Conclusion】 The publicity of Rh-HDN for early prevention and treatment should be strengthened to improve the cure rate and the prognosis.