OBJECTIVE: To optimize the preparation technology of Buqi tongmai capsule.METHODS: Orthogonal experiment of exeracting Radix Astragali was conducted with crude drugs soaking time,extracting times,amount of water added and decocting duration as factors and the content of astragaloside as index.Orthogonal experiment of exeracting Salvia miltiorrhiza,Ligusticum chuanxiong,Fructus Aurantii Immaturus,Carthamus tinctorius was conducted with crude drugs soaking time,amount of water added and decocting duration as factors and the content of tanshinol as index.RESULTS:Water was adopted for extraction and the concentration of alcohol for precipitate was 70 percent.The optimal technology of exeracting Radix Astragali was to soak the crude drugs for 30 min,decoct for 90 min in 10 times of water for 2 times.The optimal technology of exeracting S.miltiorrhiza,L.chuanxiong,Fructus Aurantii Immaturus,C.tinctorius was to decoct the crude drugs for 90 min,8 times of water for 2 times.CONCLUSION:The preparation processes is feasible,stable and meet the demands in the clinic.

BACKGROUND: Osteoporosis has been one of the main reasons for old people' s in decline living standard and shortening of lifespan. The onset of osteoporosis is related to the loss of functional coupling in osteoblast and osteoclast. So observation of the regulative effect of medicines on osteoblast is a good method to evaluate their preventive and therapeutical efficacy in osteoporosis.OBJECTIVE: To investigate the effects of genistein on osteoblast culture in vitro.DESIGN: A randomined, controlled and single-blind study based on rat osteoblasts.SETTING: Department of laboratory in amunicipal hospital.MATERIALS: The experiment was completed in the Room of Isotope, Department of Nuclear Medicine, China-Japan Friendship Hospital of Beijing from February to December 2001. Ten SD rats of 24 hours old were provided from Beijing Weitong Lihua Experimental Animal Ltd. Co. (license:SCXK11-00-0008 ). The laboratory was SPF grade.METHODS: Skull osteoblasts of rats were selected to culture in vitro. Microculture tetrozolium, p-nitrophenyl phosphate, atomic absorptiometry and alizarin red were used to observe the effect of genistein on proliferation,activity of alkaline phosphatase(ALP), contents of matrix calcium and the number of mineral nodes of osteoblasts cultured in vitro.MAIN OUTCOME MEASURES: Morphological observation, determination of proliferation ratio, ALP stain, determination of matrix calcium accumulation and number of mineralized nodes with alizarin red stain.RESULTS: It was found that genistein stimulated the proliferation of osteoblasts, improved the ALP activity, and increased the contents of matrix calcium and the number of mineral nodes in cultured osteoblasts.CONCLUSION: Genistein has the effects on stimulating the proliferation,differentiation, maturation and mineralization of osteoblasts cultured in vitro.

OBJECTIVE: To investigate the therapeutic effects of the image navigation system in nasal endoscopic surgery in treatment of basicranial tumor. METHOD: Retrospective analysis was carried out in 17 patients with basicranial tumor who underwent nasal endoscopic resection via image navigation system. The accuracy of image navigation system, operation procedure time, curative effect and complications were analyzed and summarized to evaluate the effect. RESULT: The image navigation system provided an accurate anatomical localization 0.5-2.0 mm localization error (mean 1.2 mm). The operation procedure time was 1.5-3.0 h. Postoperative complications occurred in 3 of 17 patients including cerebrospinal fluid rhinorrhea and injury of sieve plate. Two patients recrudesce within half a year. CONCLUSION The image navigation system in combination with a nasal endoscope is helpful in the resection of basicranial tumor with minimal trauma, increasing the curative effect and avoiding complications.
Cerebrospinal Fluid Rhinorrhea ; Endoscopy ; Humans ; Nasal Surgical Procedures ; methods ; Neoplasm Recurrence, Local ; Neoplasms ; surgery ; Nose ; Postoperative Complications ; Retrospective Studies ; Skull Base ; surgery ; Surgery, Computer-Assisted ; methods

Objective To observe the effect of physiotherapy on knee osteoarthritis. Methods 40 knee osteoarthritis patients were dividedinto treatment group (n=20) and control group (n=20). The control group accepted conventional therapy, acupuncture and moxibustion,fumigation, massage, etc. The treatment group accepted physiotherapy in addition, including strength training and joint activity training.They were assessed with Lysholm Knee Scoring Scale (LKSS) and Visual Analogous Scale (VAS). Results The scores of LKSS and VASimproved in both groups after treatment, and improved more in the treatment group than in the control group (P<0.01). Conclusion Physiotherapycan further improve the stability of knee and release the pain in knee osteoarthritis patients.

OBJECTIVE: To investigate the therapeutic effects of coblation treatment via endoscopy in epiglottic benign tumors. METHOD: Retrospective analysis was carried out in 128 patients with epiglottic benign tumors who underwent coblation treatment via endoscopy. The complications and therapeutic effect were analyzed. RESULT: The effective rate of treatment was 100% in cyst of epiglottis and in papillary epithelioma, and was 96.36% in hypertrophy of lingual tonsils. The pseudomembrane 1 w after operation. Postoperative pain is slight. There were no complications such as dyspnea, bleeding and infection. CONCLUSION Radio frequency coblation via endoscopy will be applicable in the therapy of epiglottic benign tumors.
Carcinoma, Papillary ; surgery ; Catheter Ablation ; Cysts ; Endoscopy ; Epiglottis ; pathology ; surgery ; Humans ; Hypertrophy ; Laryngeal Neoplasms ; surgery ; Pain, Postoperative ; Retrospective Studies

OBJECTIVE: To investigate the therapeutic effects of 768 coblation treatment in children with obstructive sleep apnea-hypopnea syndrome. METHOD: A review of 768 coblation treatment in children with obstructive sleep apnea-hypopnea syndrome was presented with respect to the sleep monitoring results, therapeutic effects and complications. RESULT: The time of pseudomembrane ablate was 10-14 d after operation. There were 11 patients occurred Secondarily bleeding within 1 week and 21 patients lymphatic tissue hyperplasia compensatorily within half a year after operation. After operation 3 months, the sleep Monitoring results were improved remarkably (P < 0.01) and the symptoms of snore and choke improved. CONCLUSION Coblation treatment in children with obstructive sleep apnea-hypopnea syndrome can reduce bleeding volume and improve obstruct syndrome apparently. The treatments of tonsils and adenoids differ with the characteristics of children and tympanotomy was fit for chil dren with secretory otitis media.
Catheter Ablation ; Child ; Child, Preschool ; Female ; Follow-Up Studies ; Humans ; Male ; Retrospective Studies ; Sleep Apnea, Obstructive ; surgery

Objective: To investigate the effect of ceramic bovine bone (CBB) combined with hydrogel complex (CBB/HG), ?-tricalcium phosphate (?-TCP) combined with hydrogel complex(?-TCP/HG) on the osteogenesis of bone marrow stromal cells (MSCs) in vitro. Methods: MSCs(induced by 50 mg/L ascorbic acid,10 -8 mol/L dexamethasone and 0.01 mol/L ?-sodium glycerylphosphate) were cultured on CBB/HG , ?-TCP/HG,CBB and ?-TCP respectively. 5 and 10 days after set up, marrow stromal cell attachment and morphology were observed by scanning electronic microscope, the proliferation of the cells was evaluated by cell counting, Alkaline phosphatase(ALP) activity was tested,typeⅠcollagen synthesis were examined by immunohistochemistry stainning. Results: On day 5 and 10 the marrow stromal cells on CBB/HG and on ?-TCP/HG were significantly more than those on CBB or ?-TCP. TypeⅠcollagen synthesis and ALP activity of the cells on CBB/HG and ?-TCP /HG were not different but significantly higher than those on CBB or ?-TCP. Conclusion: CBB/HG and ?-TCP /HG may stimulate bone marrow stromal cell proliferation, differentiation and extracellular matrix synthesis.

Objective To explore the feasibility and clinical efficacy of hand assisted laparoscopic splenectomy (HALS) for ruptured spleen. Methods From January 2002 to October 2002, HALS was performed in 12 cases, among whom were 8 cases combined with other viceral trauma. Results HALS was accomplished successfully in 10 cases and laparotomy in 2. In 10 cases, the operation time of HALS averaged 102.5 minutes. The mean post operative hospitalization was 5.8 days in 4 cases with only spleen trauma and 15.5 days in 6 combined with other viceral trauma. Conclusions HALS is a feasible, effective and safe surgical procedure for the cases with Buntain CT scalingⅡ or Ⅲ spleen trauma with no severe combined trauma.

Based on the existing situation of preventive medicine in our country,this article gives insights into the problems and the resolves of college preventive medical education in the field of society,colleges,teaching materials,teachers,students and teaching conditions.

Objective o explore intraperitoneal human fluorine safe release chemotherapy after radical surgery on the prognosis of patients with gastric cancer.Methods One hundred gastric cancer patients who were treated in Jinshan Hospital Affiliated to Fudan University from June 2011 to September 2012 were selected and divided into observation groups (gastrectomy + Human fluorine An chemotherapy) and control group (simple gastrectomy) according to a random number table method.Biochemical index before treatment and 7 d after treatment,complication occurrence and survival rate of two groups were compared.Results There were no statistically significant differences in terms of WBC,PLT,IBIL,Cr and other biochemical markers before and after treatment between the two groups (P＞0.05).There were statistically significant differences in terms of chemical peritonitis,intestinal obstruction,fistula,intestinal perforation and other complications between two groups (P＞0.05).The 6 months,1 year,2-year survival rates of control group were 87.50％,80.00％,62.50％,of observation group were 96.67％,90.00％,78.33％,the differences were significant (x2 =6.108,8.796,4.579;P=0.014,0.003,0.032).The 6 months,1 year,2-year free survival rates of control group were 75.00％,55.00％,35.00％,of observation group were 93.33％,86.67％,70.00％,the differences were significant (x2 =3.902,6.635,5.203;P =0.045,0.010,0.021).Conclusion Intraoperative intraperitoneal chemotherapy sustained radical surgery in patients with no adverse effects on the stomach,can effectively improve the postoperative survival rates,worthy of promotion.