0.05).The metastasis rate in patients with both C-erbB-2(+) and MDR1(+) was higher than the others.Their prognosis was negative.The metastasis rate in patients with both C-erbB-2(+) and Nm23(-) was higher than the others.Conclusion There are correlation between C-erbB-2,Nm23,MDR1 expressions and metastasis of breast cancer.Multi-predictors are better guide to patients than single predictor.

In modern society,it is very important to prevent and control abrupt public health incidents.But the current education programs lack the relevant contents.Some tendencies exist:stressing disease prevention but lacking injury prevention;stressing techniques in hospital but lacking skills on scene;stressing test method but lacking information technology;stressing ordinary prevention but lacking measures in unexpected situations;stressing ability of techniques but lacking ability of organization,etc.We should integrate the pertinent knowledge of abrupt public health incidents into training programs for students of public health.

OBJECTIVE:To investigate the pharmacotherapy for Parkinson disease(PD)and the factors influencing the choice of the treatment schemes for the PD patients.METHDOS:A total 269 patients with PD were assigned to 3 groups:Group I(Dopamine Agonist or Antan),Group Ⅱ(L-dopa),and Group Ⅲ(L-dopa plus Dopamine Agonist).The possible relative factors such as patients' age and sex,pathogenetic condition,educational level,occupation and income etc were analyzed statistically.RESULTS:The P value of the likelihood ratio test concerning the investigated factors such as age,sex,pathogenetic condition,education level,occupation and income were all less than 0.05 among patients treated with different kinds of therapy.The logistic regression analysis showed that all the six factors mentioned above had influence on the choice of therapy.CONCLUSION:The PD patient's selection of therapy was affected by his age and sex,pathogenetic condition,education level,occupation and income.

In the present study an experimental animal model of closed chest cardiac contusion in dogs was successfully established by impacting precardial area at the velocity of 9- 1 - 10. 5m/s with home-made elastic cord propelled impactor, After impact, some significant abnormalities of the left and right ventricular pressure,ECG and heart rate were recorded. Cardiac contusions were demonstrated at au-topsy. The establishment of the experimental cardiac contusion model could provide the tool for further study on nonpenetrating cardiac injury.

OBJECTIVE: To establish the quality standard of Buqi tongmai capsule.METHODS:Salviae miltiorrhiza,Ligusticum chuanxiong,Borneolum Syntheticum,Citrus aurantium were identified qualitatively by TLC.The content of astragaloside A was analyzed quantitatively by TLC scanning method.RESULTS: The specificity of S.miltiorrhiza,L.chuanxiong,Borneolum Syntheticum and C.aurantium could be analyzed qualitatively by TLC.The linear range of astragaloside A were 0.274 2～4.934 7 ?g (r=0.998 9) with an average recovery of 96.56%(RSD=2.70%,n=5).CONCLUSION: Established quality standard can be used for the quality control of Buqi tongmai casuple.

Team building and teamwork mode have become the important parts in scientific research and clinical approach of critical care medicine (CCM). Team is a group composed of several individuals with different abilities, complementary roles and mutual dependence based on the common goal. Teamwork mode refers to that each member of the team, as a part of the community, completes the common goal through synergy based on the common knowledge system, according to the unified way of thinking. With the characteristics of CCM and the improvement of knowledge system and thinking system, the team building and teamwork mode of CCM has experienced a chaotic period, a molding period, an excitation period and a mature period. The team building and teamwork mode provide a strong internal impetus for the development of CCM.

WANG Qing-ren's opinion of "brain governs spirit" is different from the traditional opinion of "heart governs spirit" in Chinese medicine. Analysis on his theoretic foundation and demonstration process in ‘Correction on Errors in Medical Classics’ shows that his opinion on spirit is the logical conclusion of denying "heart storing spirit" and an organic part of his idea of human body;On the other hand,it reflects the partial insistence and superficial understanding of visible evidence. WANG Qingren's opinion of "brain governs spirit" shall not be isolated from his medicine theory,but an epitome of it.

OBJECTIVE To provide the evidence in decreasing the rate of hospital-acquired infection by studying the patients in NICU and analyzing the bacteria strains isolated from these patients.METHODS We studied 512 cases from 1027 patients in NICU of Department of Neurology,813 isolated bacteria strains and their sensitivities to antibiotics were investigated.RESULTS NICU patients had a relatively high rate of hospital-acquired infection(49.85%),respiratory and urinary systems were the most common affected.Klebsiella pneumoniae was the top one species in Gram-negative bacteria(62.61%).Pseudomonas aeruginosa was the top one species in Gram-positive bacteria(28.16%).CONCLUSIONS Laboratory examination,appropriate antibiotics and precise medical behavior are the most important factors in preventing the hospital-acquired infection in NICU.

OBJECTIVE To investigate 11200 strains isolated from the patients of our hospital between from 1 Jan 2005 to 31 Dec 2006,to detect the distribution and resistance of bacteria and provide laboratory evidence for the application of antibiotics in clinics.METHODS The sensitivities of bacterial strains to antibiotics were analyzed.RESULTS SAU was the top one in Gram-positive bacteria(G+ bacteria),the occurrence of meticillin-resistant Staphylococcus aureus(MRSA)statistically increased(from 70.0% to 76.0%).Pseudomonas aeroginosa was the top one in Gram-negative bacteria(G-bacteria)(from 11.8% to 12.9%),the occurrence of ESBLs-producing Esherichia coli statistically increased(from 30.5% to 35.5%).The percentages of fungal strains isolated in the two years of 2005 and 2006 were 13.4% and 19.2%,respectively Candida albicans was the main strain among them.CONCLUSIONS These pathogens are still the common types in the patients from our hospital.It should be noted that MRSA and ESBLs-producing E.coli strains are significantly increasing and presenting resistance to a varieties of antibiotics.

Objective:To explore the changes of chemotherapy sensitivity of breast cancer MCF-7/ADM cells after Hiwi gene targeting silence, and to illuminate the role of Hiwi gene in resistant mechanism of breast cancer MCF-7/ADM cells.Methods:The MCF-7/ADM cells were transfected with Hiwi control,siRNA1 Hiwi gene and siRNA2 Hiwi gene as Hiwi control group, Hiwi siRNA1 group and Hiwi siRNA2 group.The expression levels of Hiwi gene mRNA and protein in breast cancer MCF-7/ADM cells were detected by Real-time PCR and Western blotting methods to judge the transfection rates in various groups.After transfection,the breast cancer MCF-7/ADM cells in various groups were treated with the different concentrations(0,0.1,0.5 and 1.0 mg·L-1) of adriamycin, and the cell resistant sensitivities were detected by MTT method.Results:Compared with control group(0 mg·L-1 adriamycin), the expression levels of Hiwi gene mRNA and protein inMCF-7/ADM cells in Hiwi siRNA1 and Hiwi siRNA2 groups were significantly decreased (P<0.01).Compared with control group, the survival rates of Cells in Hiwi siRNA1 and Hiwi siRNA2 groups were significantly decreased after treated with different concentrations of adriamycin,and the survival rates in Hiwi siRNA1 and Hiwi siRNA2 groups were significantly decreased(P<0.01);they were (48.15±6.28)% and (41.73±6.17)% when the concentration of adriamycin was 1 mg·L-1,and the sensitivities to the adriamycinwere obviously enhanced(P<0.01).Conclusion:The target silencing Hiwi genes in MCF-7/ADM cells is efficient.The sensitivity of MCF-7/ADM cells to adriamycin restores after Hiwi gene silencing.