No abstract available.
Humans ; Transcription Factors*

PURPOSE: Ewing's sarcoma is characterized by accompanying specific chromosome translocations. In the present study, we report ETV1, the third member of the human ETS family that is implicated in Ewing's sarcoma by reciprocal translocation (7;22)(p22;q12). METHODS: Primarily, cDNA library made from the Ewing's sarcoma cell line accompanying the t(7;22)(p22;q12) was screened using the 5' portion of EWS(including the exon 1-7) probe on the presumption that 5' portion of the fusion gene is made of EWS and 3' is a new member of the ETS family gene. After we obtained a 3' portion of ETV1, a member of the ETS family gene, an additional 5' portion was isolated by screening a human fetal brain cDNA library, using a 326 bp NcoI fragment derived from 3' portion of coding and uncoding sequences of this gene. RESULTS: We cloned the whole sequence of ETV1. Based on extensive homology, ETV1 could be subdivided into human PEA3 sub-family with ERM and E1A-F. CONCLUSION: The cloning of new oncogenes has begun to provide insight into the molecular pathogenesis of tumor formation. In this regard, the cloning of ETV1 implicated in Ewing's sarcoma might provide a clue to understand the formation of this tumor.
Brain ; Cell Line ; Clinical Coding ; Clone Cells ; Cloning, Molecular* ; Cloning, Organism ; Exons ; Gene Library ; Humans ; Mass Screening ; Oncogenes ; Sarcoma, Ewing* ; Transcription Factors*

BACKGROUND: Angiogenin, a 14.1-kDa protein isolated from the medium conditioned by HT-29 human colon carcinoma cells, induces the angiogenesis. In contrast to the human angiogenin, thought to have one homologue, the mouse angiogenin is known to have several angiogenin homologues. During we were investigating the target genes, overexpressed by the chimeric PAX3/FKHR transcription factor, new gene closely similar to the mouse angiogenin rather than angiogenin itself was obtained. We report this Ang-vl gene to understand the process of angiogenesis by comparing the mouse angiogenin family genes. METHODS: The representational difference analysis was used to investigate the target genes over expressed by the PAX3/FKHR chimeric transcription factor. The target genes were subcloned into the pBluescriptSK + and sequenced using the 73 and 77 vector itself primers. Analyses of the completed consensus nucleic acid and peptide sequences were performed using the intelligenetics and GCG software packages as well as BLAST algorithms. RESULTS: The Ang-vl gene, including the glutamine that becomes the N-terminal amino acid by the post-translational peptidase reaction and stop codon, was obtained. CONCLUSIONS: We cloned the one member of the mouse angiogenin family genes. From the point of protein chemistry, the mechanism of angiogenin or, for that matter, of any other blood vessel inducing proteins is not yet known. However, the homologues of the angiogenin might interact each other to regulate the angiogenesls. In this regard, the Ang-vl gene provides an opportunity to understand the mechanism of angiogenesis.
Animals ; Blood Vessels ; Chemistry ; Clone Cells ; Codon, Terminator ; Colon ; Consensus ; Glutamine ; Humans ; Mice* ; Transcription Factors*