1.Adenovirus Expressing Human Interferon Inhibits Replication of Foot and Mouth Disease Virus and Reduces Fatal Rate in Mice.
Jia Qi CHU ; Su Mi KIM ; Kwang Nyeong LEE ; Jae Ku OEM ; Young Joon KO ; Hyang Sim LEE ; Yong Joo KIM ; Jee Yong PARK ; Kwang Jae KIM ; Satya PARIDA ; Yooni OH ; David J PATON ; Yi Seok JOO ; Byounghan KIM ; Jong Hyeon PARK
Journal of Bacteriology and Virology 2012;42(3):224-231
Interferon is an important cytokine that plays a critical role in the initial host defense against viral infection. Recombinant human adenoviruses expressing human interferon-alpha (Ad-HIFNalpha) or pig interferon-beta fused with interleukin-18 (Ad-PIFNbeta-IL18) were constructed and used to induce an early protective response against foot and mouth disease (FMD). To analyze the antiviral effect, bovine thyroid and porcine kidney IBRS-2 cells and ICR mice were treated with Ad-HIFNalpha, Ad-PIFNbeta-IL18, and cocktail of Ad-HIFNalpha and Ad-PIFNbeta-IL18. The survival rate of suckling mice was monitored after foot and mouth disease virus (FMDV) challenge following intra-peritoneal (IP) administration of appropriate adenovirus. Indirect antigen ELISA was performed to evaluate inhibition of FMDV replication following challenge with the FMDV O, A, or Asia 1 serotypes in vitro. These recombinant adenoviruses reduced the replication of FMDV in susceptible cells, thereby decreasing the fatality in mice, suggesting that they can be a useful control method for the early protection against FMD infection in livestock after field trial.
Adenoviridae
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Adenoviruses, Human
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Animals
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Asia
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Enzyme-Linked Immunosorbent Assay
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Foot
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Foot-and-Mouth Disease
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Foot-and-Mouth Disease Virus
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Humans
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Interferon-alpha
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Interferon-beta
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Interferons
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Interleukin-18
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Kidney
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Livestock
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Mice
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Mice, Inbred ICR
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Survival Rate
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Thyroid Gland