Purpose: The document outlines the preferred methods for collecting blood from laboratory animals and blood collection volume and frequency limits. Blood collection for experimental purposes must comply with researchers of the Drug research institute (DRI) approved protocol, including approved collection techniques, volumes, and frequencies. The Department of Pharmacology researchers train investigators in various collection techniques. The researcher may collect blood for veterinary care purposes using accepted clinical techniques ensuring volumes collected do not adversely affect animal health. Blood Collection Limits: The DRI limits one time survival blood collection to 7.5% of an animal’s blood volume in most circumstances. Serial blood sampling limit vary by species, strain and frequency of blood collection. The DRI may require monitoring for anemia (using assays such as hematocrit and/or serum protein levels) when repeated collection of larger volumes are required. Blood collected for diagnostics or other veterinary procedures must be considered when evaluating total volume available for experimental use. In all cases blood collection volumes should be limited to the minimum volume that will allow for successful experimentation or diagnostics.

Purpose: The document outlines the preferred methods for collecting blood from laboratory animals and blood collection volume and frequency limits. Blood collection for experimental purposes must comply with researchers of the Drug research institute (DRI) approved protocol, including approved collection techniques, volumes, and frequencies. The Department of Pharmacology researchers train investigators in various collection techniques. The researcher may collect blood for veterinary care purposes using accepted clinical techniques ensuring volumes collected do not adversely affect animal health.Blood Collection Limits: The DRI limits one time survival blood collection to 7.5% of an animal’s blood volume in most circumstances. Serial blood sampling limit vary by species, strain and frequency of blood collection. The DRI may require monitoring for anemia (using assays such as hematocrit and/or serum protein levels) when repeated collection of larger volumes are required. Blood collected for diagnostics or other veterinary procedures must be considered when evaluating total volume available for experimental use. In all cases blood collection volumes should be limited to the minimum volume that will allow for successful experimentation or diagnostics.

Background: Herbal medicines continue to be widely used as natural promoters of good health, as immune-modulators in recent years. This situation is directly related to the rapid growth of natural based products, the decrease of chemical synthesized products and as well as the increase of natural substance consumption. Objective: The purpose of this survey was to study influence of Immunos herbal medicines on immune system in the experimental and preclinical circumstances. Materials and Methods: The immune deficiency was to created by Azathioprine through 5 days in the white mice after that control group, preparation of Immunal, Salimon and Immunos 1, 2 were administrated appropriate doses by oral during 10 days. Then we collected blood and quantified number of white blood cells (K/µL), quantity of splenocyte (×106 cell/ml), amount of CD4+, CD8+ and IgM, IgA, Ig G (mg/ml) (Elisa Kit Assay: Catalog. No: WAM-568 (Elisa Reader, 450 nm)-WKEA MED SUPPLIES CORP) on the 5th, 10th days. Results: All statistical analyses were conducted with SPSS version 20.0 software (IBM, Armonk, NY). One-way ANOVA was used to assess statistical significance between Immunos groups and days of observation. Mean values of white blood cells in blood, quantity of splenocyte, CD4+, CD8+ and IgM, IgG levels determined in the control and experimental groups. White blood cells level were significantly increased in the Immunos group compared with the control group by 55.6 percent (11.5±0.9 K/µL vs 5.1±0.51 K/µL, p<0.001) and number of splenocyte increased Immunos group compared with the control group by 60.6 % (352.2±23.5 ×106 cell/ml vs 138.6±23.5 ×106 cell/ml, p<0.01). Therefore, CD4+, CD8+ and IgM, IgG levels were significantly increased in the Immunos group compared with the control group by 0.71 to 8.8% (IgG: 11.47±0.42 vs 10.45±0.43 μg/ml, IgM: 11.33±0.81 vs 10.48±0.31 μg/ml, CD4+: 10.44±0635 vs 10.04±0.372 U/ml, CD8+: 9.75±1.02 vs 9.68±0.45 U/ml p<0.02). Conclusion It’s concluded that, Immunos preparation shows immune-stimulator effect in cellular immunity and humoral immunity in the case of immunosuppressant by Azathioprine.

Introduction: Constipation is highly prevalent, often chronic gastrointestinal disorder that affects adults. The treatment with classic drugs did not cut, in one hand with the inadequate relief of bloating and other symptoms, and with the luck of efficacy in relieving constipation. Therefore, the search for novel safe laxative drugs seems, inevitable. Rheum undulatum L. was traditionally used in constipation, thus we have attempted to evaluate the laxative effect of Rheum undulatum L. Purpose: The laxative effect of Rheum undulatum L. was evaluated against loperamide induced constipated rats. Methodology: Fifteen male normal rats were used in this study. Fifteen male constipated wistar albino rats weighing 180-250 g were also used for the study and randomized into three groups (n=5) in each of the experiments. Constipated control group rats oral administrated distilled water. Constipated rats (treatment groups) were treated with 4.1 mg/kg dose body weight /day of the preparation for one day and also Laxing a standard drug was used for the reference group. The fecal weight, the fecal humidity laxative activity were monitored in experimental rats. Results: Constipation was successfully induced in the rats by loperamide as seen in the elevated fecal properties compared to the control rats. The Rheum undulatum L. compounds preparation administered orally produced significant laxative activity and reduced loperamide induced constipation in dose dependent manner as seen in the increase of fecal output. The same doses of the Rheum undulatum L. compounds preparation produced a significant increase (P<0.05) fecal weight, the faeces humidity. The effect of the compounds preparation compares favourably well with Laxing, a standard laxative drug. Conclusion The results of this study justify the use of Rheum undulatum L. compounds preparation as a laxative in traditional medicine. The produced significantly increase in fecal output of rats and the stimulation of gastrointestinal motility.

Introduction: Shilajit and Rhodiola Rosa L are widely used in Mongolian Traditional medicine for the management of diseases and for fracture healing. The aim of this study was to evaluate the pharmacology effects of the “Vitos” Shilajit Shot preparation on fracture healing and callus stages in rats by X-ray. Material and methods: We used non-liner Wistar rats for in vivo experiments, there are sixteen rats were randomly grouped as a positive control, negative control, “Vitos” Shilajit shot experimental and standard groups. The positive group was as healthy animals and other groups were created femoral fracture by Bonnaren’s device. Then negative control group was oral administered distilled water, whereas 4.1ml/kg of “Vitos’ Shilajit shot administrated via oral gavage to experimental group through 56 days. X-rays were performed to assess fracture healing effects within 14, 28, 42, 56 days and callus stages. Results: Significantly higher callus volume and callus staging were observed in the “Vitos” Shilajit shot group compared with the negative control and standard groups. Also “Vitos” Shilajit shot group was becoming as bridging between both end of fractures and get hard callus formulation ready observation of X-Ray radiograph on 4 weeks post fracture. The fracture healing process was slightly reached to callus remodulation such as final stage of bone formulation on 56^th day. Conclusion The results of this study reveal that, “Vitos’ shot preparation, which contains an extract of Rhodiola Rosa L and thick extract of Shijilat has a treatment effect and enhancing and supporting callus of bone fracture healing.

Introduction: During many decides, compounds derived from natural raw materials have demonstrated their effectiveness as therapeutic agents in different areas, such as metabolic disorder, immune system diseases and its regulations. Natural based products, like herbal medicines and minerals are implicated in the regulation of immune function. They control the immune system in a pleiotropic manner and participate in various processes of the adaptive/innate immunity. Therefore, natural raw material has great potential for targeted immune modulators, in the treatment of certain types of immunologic and inflammatory diseases, like rheumatoid arthritis, plaque psoriasis, ankylosing spondylitis and immune deficiency. The purpose of this survey was to study influence of “Shilajit +Golden Rosa” combined shot preparation named by Vitos on immune system in the experimental and preclinical circumstances. Goal: The purpose of this survey was to study influence of “Shilajit +Golden Rosa” Vitos shot preparation on immune system in the experimental and preclinical circumstances. Material and Methods: The immune deficiency was to created by Azathioprine through 5 days in the Balb/c mice after that control group, preparation of “Shilajit +Golden Rosa” Vitos shot were administrated appropriate doses by oral during 10 days. Then we collected blood and quantified amount of CD4+, CD8+, IgG and CD64 (Mouse Elisa Kit Assay: Catalog.No:WAM-568, Elisa Reader, 450 <b>нмb>, Melsin Medical Co.LTD, www. melsin.com) on the 5^th, 10^th days. Results: All statistical analyses were conducted with SPSS version 20.0 software (IBM, Armonk, NY). Oneway ANOVA was used to assess statistical significance between “Shilajit +Golden Rosa” Vitos shot group and days of observation. Mean values of CD4+, CD8+, CD4/CD8 ratio, IgG, CD64 levels determined in the control and sample group. CD4+, CD8+, CD4/CD8 ratio, IgG and CD64 levels were significantly increased in the “Shilajit +Golden Rosa” Vitos shot group compared with control group by 20.8-67.8 per cent (p<0.05, p<0.01). Conclusion It’s concluded that, “Shilajit +Golden Rosa” Vitos shot preparation shows immune-stimulator activity not only in the level of cellular (T cells:CD4+, CD8+) but also humoral immunity (B cells: IgG, CD64) in the previously using Azathioprine (75mg/kg) to provoke pathological model of immunosuppression