Objective To investigate the relationship between serum uric acid(SUA)and clinic characteristics and result of coronary artery angiography of patients with coronary heart disease(CHD).Methods Two hundreds and seven patients for coronary artery angiography were divided into CHD group and control group.Their clinical data and results of coronary artery angiography were analyzed through statistics.Results SUA levels of CHD patients were significantly higher than the control subjects SUA(P

Objective To evaluate the urinary fluorine detection quality of endemic fluorosis monitoring network laboratories in Guizhou Province and promote the improvement of quality control.Methods From 2010 to 2012,according to the method for determination of fluoride in urine by ion selective electrode,in 11 endemic fluorosis monitoring network laboratories in Guizhou,an quality control assessment was done.In the internal quality control assessment,standard substance detection inspection was used for personnel parallel samples and equipment comparison,t test was used to compare the inter-group differences.In the external quality control inspection,blind urine fluorine sample was tested,and a Z score value evaluation method was used to compare the relative error of the test results.Results In the assessment of internal quality control,the parallel comparison test results of sample,personnel and instrument showed that the differences were not statistically significant (t =-1.81-1.80,P ＞ 0.05).In 2010,in the external quality control inspection,there was 1 laboratory |Z|≥3,and 2 test results |Z| ＞2;in 2011,there was 4 test results |Z| ＞ 2;in 2012,there was 5 test results |Z| ＞ 2;in 2010,there was one laboratory in a blind test,relative error of the results ＞ 20％;in 2011 and 2012,there was a laboratory in a blind test,relative error of the results ＞ 10％.Conclusion The endemic fluorosis monitoring network laboratory testing ability in Guizhou is relatively good,but there is an obvious individual laboratory system error.

Objective Through investigation of children fluorosis illness,family households improved stoves and related life styles,to provide a scientific basis for sustainable control of endemic fluorosis.Methods In 2013,in Huishui County and Baiyun District,3 towns were selected in each county(district),and 3 villages were selected in each town.All 8-12 years old children in the school of these villages were checked dental fluorosis,which was diagnosed according to Dental Fluorosis Diagnosis (WS/T 208-2011); at the same time,10 families were selected to survey the situation of improved stoves and related life styles.Results Dental fluorosis detection rate of 8-12 years old children in Huishui and Baiyun were 2.75％ (23/836) and 2.26％ (11/487),which were all lower than 30％.Qualified rate of improved stoves and qualified stoves correct utilization rate were all 100.0％ (90/90).For human consumption,the correct rate of corn drying was 100.0％ (90/90) ; the correct rates of chili drying were 98.9％ (89/90) and 100.0％ (90/90).Conclusions The prevention effect is obvious,which has reached the control standards.We should continue to improve the long-term mechanism of comprehensive control measures,and to achieve substantial elimination of coal-burning endemic fluorosis.

Objective To evaluate the effectiveness of renal sympathetic denervation (RDN) for hypertensive heart dis-ease combined with systolic heart failure. Methods Two patients (mean age 35 years) with hypertensive heart disease com-bined with systolic heart failure on maximal tolerated heart failure therapy underwent bilateral renal denervation. Echocar-diography, the six minute walk distance, renal function, glycosylated hemoglobin and NT-proBNP were assessed at baseline and 1 year after renal denervation. Results Renal artery angiography showed that no stenosis and dissection. After 1 year follow up, the left ventricular ejection fraction (LVEF), six minute walk distance and NT-proBNP were significantly im-proved, and the size of left ventricular decreased. Conclusion RDN is effective and feasible for the treatment in patients with hypertensive heart disease and systolic heart failure.

Objective To observe the effects of comprehensive prevention and control measures through the trend change analysis of the prevalence of dental fluorosis of children and urinary fluoride in Guizhou.Methods Three villages were selected in each county from Qianxi,Pu'an and Renhuai,in 2009,2011 and 2013.Dental fluorosis of all children aged 8-12 were examined using the reference Diagnosis of Dental Fluorosis (WS/T 208-2011);50 children were selected randomly from each village to detect urinary fluoride content according to the reference Determination of Fluoride in Urine-ion Selective Electrode Method (WS/T 89-1996).Results The detectable rates of dental fluorosis of children aged 8-12 from Qianxi,Pu'an and Renhuai were 53.20％ (490/921),89.97％ (547/608) and 51.73％ (629/1 216),respectively,in 2009,46.68％ (281/602),63.60％ (332/522) and 43.84％ (530/1 209),respectively,in 2011 and 34.04％ (256/752),14.29％ (80/560) and 26.97％ (243/901),respectively,in 2013,and the detection rate in each county was reduced year by year (x2 =60.46,674.96,125.95,all P ＜ 0.01).In 2009,3 counties' urinary fluoride levels of children aged 8-12 were (1.21 ± 1.29),(0.89 ± 0.80) and (1.00 ± 0.59) mg/L,respectively,which were significantly higher than those of 2013 [(0.94 ± 0.52),(0.68 ± 0.26) and (0.87 ± 0.33) mg/L,t =3.743,4.778,3.743,all P ＜ 0.01].Conclusions The dental fluorosis prevalence and urinary fluorine content of children aged 8-12 are falling obviously through comprehensive prevention and control measures.The target to control and eliminating the disease of endemic fluorosis is not reached.It should be continue to strengthen management after comprehensive prevention and control measures.

Cerebral microbleeds are new biomarkers of the cerebral small vascular disease.With the development of neuroimaging techniques,the related research of cerebral microbleeds has been paid more and more attention.The research of the correlation between cerebral microbleeds and cognitive impairment and stroke,imaging features,pathological changes,and clinical outcomes can provide an important basis for clinical treatment decisions.

Objective To analyze and explore the value of the mutislice spiral CT (MSCT) scanning,its reconstructive technology and MRI scanning for applying to the identifying diagnosis of new and old vertebral compresion fracture.Methods One hundred and sixty-seven cases with the new and old vertebral compresion fracture who have 189 vertebrae in total were examined using the MSCT scanning,their imaging information were postprocessed with 2D and 3D reconstruction at the work station.Thirty-four patients with overall 43 vertebrae were carried out by MRI scanning and their images were compared with those of MSCT.Results They had the highest proportion of vertebrae in L1 with accounting for 27.68% (31/112) and 35.06% (27/77) among the patients with new and old vertebral compresion fracture,respectively.The next was T12,L2 and T11.There were statistically significant differences between new and old cases in such appearance as fracture line clear and sharp(102 and 21),contusion and hemorrhage of spinal cord(15 and 0),parenchyma shadow beside vertebrae(103 and 11),appendant fracture(26 and 5),organ's lacerated wound around vertebral body(30 and 0),discus intervertebrales vacuum(10 and 36),derangement and hardening of vertebral bone trabecularism(29 and 51) (P＜0.01).Twenty-six vertebrae with the uneven low T1 WI signal,27 vertebrae with the T2 WI fat-suppresion irregular high signal were demonstrated in MRI scanning among 27 vertebrae of 21 cases with new vertebral compresion fracture.While 16 vertebrae with the T1WI and T2WI signals were the same as those of the normal vertebrae,16 vertebrae were the T2WI fat-suppresion low signal among 13 cases with old vertebral compresion fracture.Conclusions The MSCT plays an important role in identifying diagnosis for the most of the patients with the new and old vertebral compresion fracture, while MRI scanning may reflect the pathophysiological characteristics in diagnosis of the spinal cord and the ligament lesion.Therefore,the MSCT and MRI scanning should be integrated to be used to enhance remarkably the accurate rate of the diagnosis.

To elucidate arsenic-induced oxidative DNA damage, the genotoxicity of arsenic in human cells was comparatively studied with single cell gel electrophoresis (SCGE) assay in combination with the observation of the protective effects of dimethyl sulfoxide (DMSO) and catalase. Arsenic, at the concentration of 2.4 μM by coincubation for 24 hours, significantly induced DNA damage in HL60, a human promyelocytic leukemia cell line. In contrast, significant DNA damage was found in human mononucleocytes at the concentration of 4.8 μM or above. The cells were incubated separately with DMSO (12 mM/l), a well-known hydroxyl radical (OH-) scavenger, and catalase (1,300 U/ml), a hydrogen peroxide (H2O2) scavenger, for 6 hours and then further coincubated with various concentrations of arsenic for 24 hours at 37°C and 5% CO2. The findings showed that both DMSO and catalase significantly reduced the arsenic-induced tail moment, a parameter of total damaged DNA, in HL60 and mononucleocytes. Hence our findings indicate that arsenic, with micromolar concentrations, induces typical and various extents of DNA damage in human cells via reactive oxygen species in a dose-dependent manner.
Human ; DNA Damage ; Arsenic ; Dimethyl Sulfoxide ; Arsenic measurement

Objective To investigate the value of CT and endoscopic ultrasonography (EUS) in the diagnosis of gastrointestinal stromal tumors (GIST). Methods Eleven patients with surgically and pathologically proved GIST were collected and retrospectively analyzed.Plain CT scanning was performed in all 11 cases and enhancement in 10 cases, while additional EUS in 8 cases.Results Of the 11 lesions, 8 were located in stomach, 1 in esophagus, 1 in duodenum, and 1 in rectum, respectively.In benign GIST, the diameter of the tumor was less than 5 cm, with clear margin and homogeneous density.In malignant GIST, the characteristic appearances were as follows: (1) The diameter of the tumor was more than 7 cm; (2) The tumor was lobular in shape; (3) Lesions contained intratumoral small or large patchy necrosis; (4) The tumor had large and deep ulcers; (5) Around the tumor, small vessels arranged in clumps or in line; (6) The tumor contained scattered small calcifications; (7) Invasion of adjacent tissues and metastasis.The positive predictive value of CT for location of GIST was 54.5% (6/11), the positive predictive value of EUS was 87.5% (7/8).By pathology, benign GIST was identified in 3 cases, malignant in 7 cases, and active proliferation in 1 case.The positive predictive value of CT for differentiation of benign and malignant GIST was 72.7% (8/11), the positive predictive value of EUS was 62.5% (5/8).Conclusion CT combined with EUS are helpful in evaluating the gastrointestinal origin of GIST, and in differentiating between benign and malignant GIST, and in instructing clinical treatment and estimating prognosis.

Guided by the principle of multimedia instruction, the author puts forward a new teaching mode, in which theoretical teaching and experimental teaching can be processed synchronously based on campus network, interactive teaching is dominative, students are the principal part, and integral optimization of theoretical teaching and experimental teaching can be realized.