2.Anti-herpes Activity of Vinegar-processed Daphne genkwa Flos Via Enhancement of Natural Killer Cell Activity.
Erdenebileg UYANGAA ; Jin Young CHOI ; Hyung Won RYU ; Sei Ryang OH ; Seong Kug EO
Immune Network 2015;15(2):91-99
Herpes simplex virus (HSV) is a common causative agent of genital ulceration and can lead to subsequent neurological disease in some cases. Here, using a genital infection model, we tested the efficacy of vinegar-processed flos of Daphne genkwa (vp-genkwa) to modulate vaginal inflammation caused by HSV-1 infection. Our data revealed that treatment with optimal doses of vp-genkwa after, but not before, HSV-1 infection provided enhanced resistance against HSV-1 infection, as corroborated by reduced mortality and clinical signs. Consistent with these results, treatment with vp-genkwa after HSV-1 infection reduced viral replication in the vaginal tract. Furthermore, somewhat intriguingly, treatment of vp-genkwa after HSV-1 infection increased the frequency and absolute number of CD3-NK1.1+NKp46+ natural killer (NK) cells producing interferon (IFN)-gamma and granyzme B, which indicates that vp-genkwa treatment induces the activation of NK cells. Supportively, secreted IFN-gamma was detected at an increased level in vaginal lavages of mice treated with vp-genkwa after HSV-1 infection. These results indicate that enhanced resistance to HSV-1 infection by treatment with vp-genkwa is associated with NK cell activation. Therefore, our data provide a valuable insight into the use of vp-genkwa to control clinical severity in HSV infection through NK cell activation.
Animals
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Daphne*
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Herpesvirus 1, Human
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Inflammation
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Interferons
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Killer Cells, Natural*
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Mice
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Mortality
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Simplexvirus
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Therapeutic Irrigation
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Ulcer
3.Chemical constituents of Daphne odora var. margirmta.
Jing PENG ; Yanying YU ; Wen XIONG ; Chunpeng WAN ; Shuwen CAO
China Journal of Chinese Materia Medica 2011;36(10):1316-1318
OBJECTIVETo study the chemical constituents of Daphne odora var. margirmta.
METHODThe compounds were isolated and purified by column chromatography of silica gel, ODS, Sephadex LH-20, and their structures were identified by UV, 1H-NMR, 13C-NMR, MS and CD spectroscopic methods.
RESULTTen compounds were elucidated as wikatrols B (1), kaempferol (2), daphnodorin B (3), daphnodorin D2 (4), daphnodorin A (5), daphnodorin C (6), daphnodorin D1 (7), daphnodorin I (8), daphnetin (9), daphnoretin (10).
CONCLUSIONCompounds 1,2,4 were obtained from this plant for the first time,and compound 1 was isolated firstly from the genus Daphne.
Daphne ; chemistry ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Plant Roots ; chemistry
4.Isolation and identification of a new dicoumarin from the roots of Daphne genkwa.
Acta Pharmaceutica Sinica 2004;39(12):990-992
AIMTo investigate the chemical constituents of the roots of Daphne genkwa.
METHODSSilica gel and Sephadex LH-20 column chromatographic techniques were employed for the isolation and purification. The structure was elucidated by a combination of spectroscopic analyses.
RESULTSA new dicoumarin named isodaphnoretin was isolated and the structure was established as 7-hydroxy-6-methoxy-4-[(2-oxo-2H-1-benzopyran-7-yl)-oxyl]-2H-1-benzopyran-2-one.
CONCLUSIONIsodaphnoretin is a new compound.
Coumarins ; chemistry ; isolation & purification ; Daphne ; chemistry ; Molecular Conformation ; Molecular Structure ; Plant Roots ; chemistry ; Plants, Medicinal ; chemistry
5.Three biflavonoids from ethanol extract of the root of Daphne genkwa.
Acta Pharmaceutica Sinica 2005;40(5):438-442
AIMTo investigate the chemical constituents of the secondary metabolites of the roots of Daphne genkwa.
METHODSThe roots of D. genkwa were extracted with 95% ethanol at 60-70 degrees C for 7 days to obtain the crude extract. The crude extract was purified by silica gel and Sephadex LH-20 column chromatography as well as the HPLC techniques. The structures of the isolates were elucidated by combined spectroscopic methods including 1D and 2D NMR, MS, UV, IR and CD.
RESULTSThree new biflavonoids were isolated from the ethanol extract of the roots of D. genkwa and their structures were identified as daphnodorin H-3-methyl ether (1), daphnodorin H-3"-methyl ether (2) and daphnodorin G-3"-methyl ether (3).
CONCLUSIONCompounds 1, 2 and 3 are three new biflavonoids.
Biflavonoids ; chemistry ; isolation & purification ; Daphne ; chemistry ; Ethanol ; Molecular Conformation ; Molecular Structure ; Plant Roots ; chemistry ; Plants, Medicinal ; chemistry
6.Studies on the chemical constituents in roots of Daphne odora var. atrocaulis.
Wei ZHANG ; Wei-dong ZHANG ; Ting-zhao LI ; Chuan ZHANG ; Run-hui LIU ; Yun ZHOU ; Juan SU
China Journal of Chinese Materia Medica 2005;30(7):513-515
OBJECTIVETo study the chemical constituents from Daphne odora var. atrocaulis.
METHODThe chemical constituents were isolated and repeatedly purified by silica gel column chromatography and the structures were elucidated by the NMR spectra and physicochemical properties.
RESULTSixteen compounds were obtained and nine of them were identified as beta-sitosterol, 4-hydroxy ethylbenzoate, (2E),-2-propenoic acid,3-(3,4-dihydroxyphenyl)-decosylester, genkwanin, 2,4-dihydroxypyrimidine, daphnetin, daphnoretin, 5,7,4'-trihydroxyflavone-3ol, daucosterol.
CONCLUSIONSeven compounds were obtained from D. odora var. atrocaulis. for the first time.
Coumarins ; chemistry ; isolation & purification ; Daphne ; chemistry ; Flavones ; chemistry ; isolation & purification ; Plant Roots ; chemistry ; Plants, Medicinal ; chemistry ; Umbelliferones ; chemistry ; isolation & purification
7.Chemical constituents from rhizome of Daphne papyracea var. crassiuscula.
Ying WEI ; Guang-Yi LIANG ; Ye WANG ; Xiao-Hu JIANG ; Dao-Ping WANG ; Hattori MASAO
China Journal of Chinese Materia Medica 2012;37(22):3434-3437
OBJECTIVETo study chemical constituents from rhizome of Daphne papyracea var. crassiuscula.
METHODEthyl acetate fraction of 75% ethanol extracts from rhizome of D. papyracea var. crassiuscula, and its strucutre was identified by spectral method.
RESULTNine compounds were separated and identified as daphneticin (1), daphnetin (2), hydrangetin (3), daphnoretin (4), 1-4'-hydroxyphenyl-5-phenyl-2 (E)-en-1-pentanone (5), daphneolon (6), 3beta-O-acetyl-olean-12-en (7), and (+)-usnic acid (8).
CONCLUSIONCompounds 1-8 were separated from D. papyracea var. crassiuscula for the first time. Compound 8 was separated from the genus for first time.
Chromatography, High Pressure Liquid ; Daphne ; chemistry ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Magnetic Resonance Spectroscopy ; Molecular Structure ; Rhizome ; chemistry
8.RP-HPLC determination of tiliroside in Daphne genkwa.
Lili SONG ; Juan LU ; Peifang YAN ; Lina TAN ; Xuwen LI ; Yongri JIN
China Journal of Chinese Materia Medica 2009;34(3):301-303
OBJECTIVETo determine the contents of tiliroside [kaempferol 3-O-beta-D-(6 " -p-coumaroyl) glucopyranoside] in the Daphne genkwa.
METHODThe separation was performed on ZORBAX XDB C18 column (4.6 mm x 250 mm, 5 microm), using acetonitrile and water/acetic acid mixture (1,000/1) (gradient elution: 0-18 min, 26 : 74; 18-33 min, 80 : 20) as mobile phase with the rate of 1.0 mL x min(-1) at 25 degrees C, the wavelength for measurement was 310 nm.
RESULTThe linearity was in the range of 0.1-5.0 microg (n=6), and the correlation coefficient was 0.9999. The average recovery for compound tiliroside was 101.50% (RSD 0.90%) (n=6).
CONCLUSIONThis method is accurate, reliable and reproducible to determine tiliroside in the D. genkwa.
Acetonitriles ; chemistry ; Chromatography, High Pressure Liquid ; methods ; Daphne ; chemistry ; Flavonoids ; isolation & purification ; Oils, Volatile ; pharmacology ; Plant Stems ; chemistry
9.Chemical constituents from Daphne acutiloba.
Sheng-Zhuo HUANG ; Qing-Yun MA ; Yu-Qing LIU ; Jun ZHOU ; You-Xing ZHAO
China Journal of Chinese Materia Medica 2013;38(1):64-69
OBJECTIVETo study the chemical constituents from Daphne acutiloba.
METHODThe constituents were separated by column chromatography, and their structures were elucidated by spectroscopic data analyses.
RESULTFifteen compounds were isolated from the EtOAc extract and identified as wikstroelide M (1), vesiculosin (2), prostratin (3), 7-hydroxy-coumarin (4), 7,8-di-hydroxy-coumarin (5), isodaphnoside (6), daphnorine (7), rutamontine (8), daphnolin (9), daphneticin (10), (+)-pinoresinol-beta-D-glucoside (11), oleodapnone (12), oleodaphnal (13), ergosterol peroxide (14) and cholest-5-en-3beta-ol (15).
CONCLUSIONAll the compounds except for 4, 5 and 14 were obtained from the stems of this plant for the first time.
Coumarins ; chemistry ; isolation & purification ; Daphne ; chemistry ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Furans ; chemistry ; isolation & purification ; Lignans ; chemistry ; isolation & purification ; Mass Spectrometry
10.Correlation between chemical composition,ecological factors and soil factors of Chinese herbal medicine Daphnes Cortex.
Qi-Rui MU ; Dan JIANG ; Yuan HE ; Lu GENG ; Guang-Xi REN ; Zhen-Fang BAI ; Xu ZHANG ; Zhong-Yi ZHANG ; Chun-Sheng LIU
China Journal of Chinese Materia Medica 2020;45(5):1059-1063
In this paper, the correlation between the chemical constituents of Chinese herbal medicines Daphnes Cortex and the ecological factors and soil factors was studied, which provided a reference for the selection of suitable areas for artificial cultivation of Daphne giraldii and wild tending. The geographic information system(GIS) was applied to obtain the ecological factor information of 23 collection sites of Daphnes Cortex, and the soil factor information was determined by the standard procedure in the soil test standard manual. Combining the information of 93 chemical constituents of Daphnes Cortex in 23 collection sites the correlation between components and ecological factors and soil factors was analyzed by statistical methods. The correlation analysis showed that the longitude, annual average rainfall, annual sunshine intensity, annual average temperature in the ecological factors, soil type, effective copper and pH value were the dominant factors affecting the chemical composition of Daphnes Cortex.
China
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Copper
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Daphne/chemistry*
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Drugs, Chinese Herbal
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Geographic Information Systems
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Hydrogen-Ion Concentration
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Plants, Medicinal/chemistry*
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Rain
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Soil/chemistry*
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Sunlight
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Temperature