Objective To observe the attenuated effect of Kanglaite Injecti on (KI) combined with chemotherapeutic drugs on transplanted human lung cancer A54 9 in nude mice. Methods Ninety- six nude mice were transplanted subcutaneousl y with human lung cancer A549; 7 days later, the mice were randomized into 16 gr oups(n=6) and were given antitumor drugs according the regimen. Other 12 normal mice were used as controls. KI groups were given KI 1.25, 2.5 and 5.0 g/kg respe ctively and were administered (iv) continuously for 10 times on 7th day of trans plantation; cisplatin (DDP, 1.5 mg/mg or 3 mg/kg) and Gemzar (30 mg/kg or 60 mg/ kg) were given one dose by intraperitoneal injection on 10th day of transplantat ion; Gemzar was given again 4h after medication of DDP. Taxotere (25 mg/kg) was given one dose by intraperitoneal injection on 7th day of transplantation. The m ice were killed to examine the body weight, tumor weight and tumor- inhibiting rate. Results KI 1.25, 2.5 and 5.0 g/kg respectively combined with DDP 3mg/kg and Gemzar 60 mg/kg had a better inhibitory effect on the growth of human lung c ancer A549 than KI, DDP and Gemzar alone. KI in three doses respectively combine d with DDP 1.5 mg/kg and Gemzar 30 mg/kg not only had a better inhibitory effect than those of KI, DDP and Gemzar alone, but also promoted the antitumor actions . The tumor- inhibitory rate of KI in three doses respectively combined with Ta xotere (25 mg/kg) was higher than KI and Taxotere alone. Conclusion KI combine d with DDP and Gemzar or with Taxotere can shorten the tumor mass and has tumor - inhibitory and attenuated effect.

Objective To observe the preventive and therapeutic effect of Ka ng laite Injection (KI) on acute nephrotoxicity in rats induced by cisplatin (DDP) and gemzar.Methods Forty SD rats, male and female in a half, were randomized in to normal control group, KI group, DDP+ Gemzar group and KI+ DDP+ Gemzar gro up, 5 male and 5 female in each group. The KI+ DDP+ Gemzar group were given K I 10 mL/kg first,DDP injection 6 mg/kg 2 hours later and Gemzar injection 90 mg/ kg 4 hours later. After treatment, acute nephrotoxicity was observed.Results On e week after treatment, all of the rats survived; the body weight of the male an d female rats in group was similar in KI group and in the normal control group, but lower in DDP+ Gemzar group, in particular the male rats, than that in norma l control group. Body weight in KI+ DDP+ Gemzar group was lower than that in the normal control group but higher than that in DDP+ Gemzar group.The serum le vels of biochemical parameters were similar in KI group and the normal control g roup; serum levels of total protein (TP) and albumin(ALB) were markedly lower bu t those of blood urea nitrogen (BUN) and creatine (CRE) higher in DDP+ Gemzar g roup than those in the normal control group; the above indexes were improved in KI+ DDP+ Gemzar group as compared with those in DDP+ Gemzar group and arrive to the normal level.Conclusion KI has preventive and therapeutic effect on acu te nephrotoxicity in rats induced by DDP and Gemzar.

Objective To explore a safe and effective imaging method by the application of 3D contrast-enhanced MR Angiography (3D CE-MRA) with low-or conventional-dose contrast agent to the examination of lower extremity arterial disease.Methods Totally 31 patients suspected with lower extremity arterial stenosis or occlusion underwent 3D CE-MRA examination including one patient had right leg cut due to the disease,of whom,18 ones had conventional-dose contrast agent (38 ml) injected in 1.5T MR machine and 13 ones had low-dose agent (15 ml) injected in 3.0T MR machine.Quality evaluation was carried out for the images by different doses of agents.Results All the patients had no agent allergy occurred,and one patient had left tibia-fibula motion artifact due to involuntary movement.3.0T MR machine with low-dose agent behaved better than 1.ST MR machine with conventional-dose agent in leg imaging,while there were no significant differences between the quality of the images except that of popliteal artery (P＞0.05).Conclusion 3D CE-MRA is an effective and safe method in detecting lower extremity artery disease,and 3.0T MR machine with low-dose agent lays a foundation for clinical diagnosis and treatment.

Objective To verify the mediating effect of the moral identity on the relationship between sport moral disengagement and pro-social and anti-social behaviors in competition.Methods Two hundreds and sixty-seven athletes of group events(including 173 males,94 females,with an average age of 22.40,SD=2.75)were selected and investigated using the sport moral disengagement scale,moral identity scale and the scale of pro-social and anti-social behavior in competition.Results (1) The sport moral disengagement had significant influence on the athletes'pro-social and anti-social behaviors in competition.(2)The moral identity has significant positive influence on the athletes' pro-social behavior in competition,but significant negative influence on the athletes' anti-social behavior in competition.(3)The moral identity has significant mediating effect on the relationship between sport moral disengagement and pro-social behavior in competition.Conclusion The moral disengagement in sport has important influence on the athletes' pro-social and anti-social behavior in competition.The moral identity has a significant mediating effect on the relationship between sport moral disengagement and pro-social behaviors in competition.

The spasticity can affect the motor function, activities of daily living (ADL), quality of life (QOL) of hemiplegic patients,and also delay the rehabilitation process. Principally, the methods of reducing spasticity included physical therapy and medication at present.The massage technique has been having outstanding effect on alleviating spasticity after stroke. This article reviewed the clinical efficacy,methods and mechanisms of massage treatment for hemiplegic spasticity.

Objective To observed the analgesia and adverse reactions of continuous patients-controlled in-terscalene analgesia with patient-controlled intravenous analgesia for the shoulder arthroscopic surgery.Methods 40 patients with ASA Ⅰ ~Ⅱ anesthesia in general anesthesia for the shoulder arthroscopic surgery were selected and randomly divided into the two groups,20 cases in each group.They were continuous patients-controlled interscalene analgesia(PCNA)and patient-controlled intravenous analgesia (PCA).PCNA with 0.2% ropivacaine +saline to 100mL,background parameters 4ml/h,a single dose of 0.5mL,lock 20min.24 hours after dosing.PCA with fentanyl lmg +tropisetron 5mg +saline to 100mL,background parameters 2mL/h,a single dose of 3.5mL,lock 20min.Record the VAS score 6h,12h,24h,48h after surgery,and adverse side effects of postoperative dizziness,nausea,vomiting and itchy skin.And the patient's analgesic satisfaction survey 48h after surgery.Results PCNA after surgery 6h,12h, 24h,48h in VAS score was significantly lower than the PCA(P <0.05).PCNA were dizziness,nausea,vomiting and the incidence is lower than the PCA,the difference was statistically significant(P <0.05).The PCNA of patients sat-isfaction was 100%,the PCA of patients satisfaction is 75%,the difference was statistically significant(P <0.05). Conclusion In the postoperative analgesia of shoulder arthroscopic surgery,the analgesic effect and patient satisfac-tion of continuous patients-controlled interscalene analgesia are better than patient-controlled intravenous analgesia. And the dizziness,nausea,vomiting is lower.

Hazards of noise-induced hearing loss (NIHL) to crowd who are long-term exposured and work in the noisy environment is increasingly prominent. But just part of the individuals who are exposed to the same noisy environment have hearing loss, and the severity of hearing loss are different, which indicates genetic predisposition might be associated with NIHL. In recent years, many experts and scholars at home and abroad have done a lot of research in this field, this article summarizes all available studies.
Genetic Predisposition to Disease ; Hearing Loss, Noise-Induced ; genetics ; Humans ; Noise, Occupational ; adverse effects

Objective To explore the role and mechanism of Toll-like receptor 4(TLR4)and chemokine receptor 7(CXCR7)in the pathogenesis and development of Helicobacter pylori(HP)positive gastric cancer.Methods Tissues of 147 cases with gastric carcinoma and 60 normal control were collected.The protein expression of TLR4,CXCR7 was detected by immunohistochemical staining.HP infection in these samples were detected by immunohistochemistry and Gram staining.Results The positive rates of TLR4 and CXCR7 in gastric cancer tissues were 63.9% and 62.6%,respectively,which were significantly higher than those in normal control group(15.0% and 5.0%,respectively;X2=40.832,56.901,all P<0.01).The positive rates of TLR4 and CXCR7 in patients with lymph node metastasis were significantly higher than those without lymph node metastasis(X2=9.809,11.444,all P<0.01).The positive rates of TLR4 and CXCR7 in patients with stage Ⅲ and Ⅳ were significantly higher than those of stageⅠand Ⅱ(X2=24.927,27.642,all P<0.01).The expression of TLR4 and CXCR7 in gastric carcinoma was significantly related to HP infection.Kaplan-Meier survival analysis showed that the survival rates in TLR4 and CXCR7 positive group were significantly lower than those in TLR4 and CXCR7 negative groups(F=4.053,4.091,all P<0.05).COX regression analysis indicated that the TNM stage,the expression of TLR4 and CXCR7 were independent prognosis factors of gastric carcinoma.Conclusion TLR4 and CXCR7 are closely related to the occurrence and development of gastric cancer,and may play an important role in HP carcinogenesis.It may be involved in the invasion and metastasis of gastric cancer cells via LPS/TLR4 signaling pathway.

A polyimide coated stir bar for sorptive extraction (SBSE) was prepared by immersion precipitation method, and evaluated by using 5 phenols and chlorinated phenols as model samples. The extraction efficiency of the prepared stir bar was the highest compared with commercial extraction phases of SBSE. Experimental parameters including stir speed, ionic strength, extraction temperature, extraction time, desorption temperature and time were optimized. Under the optimal conditions such as 100 mL of sample, 30 g of NaCl, extraction time of 30 min, stirring speed of 800 r/ min and at 25℃, the target compounds were recovered by thermal desorption at 300℃ for 4 min, more than two orders of magnitude of linearity was obtained (R≥0. 9995), LOQs (S/ N=10) were 0. 028-0. 123 μg/ L, and RSDs were in the range of 1. 6% -9. 7% . The polyimide SBSE coupled with gas chromatography-mass spectrometry was applied to the extraction/ enrichment and analysis of phenols in real samples, including tap water, sea water, and waste water. It was found that the polyimide SBSE showed high selectivity towards polar compounds and high thermostability up to 350℃.

[Objective]To examine the difference of proliferation and ?-smooth muscle actin(?-SMA) expression between healing and normal medial collateral ligament(MCL) fibroblasts.[Method]Ten adult,male,Sprague-Dawley rats weighing between 350 and 375 g were used in this study.Normal and healing MCL were cut into small pieces in aseptic conditions,and then placed and cultured in culture chamber.Fibroblasts were passaged with 0.25% trypsin.After 24 and 48 hours incubation,MTT was used to measure the cell proliferation.The production of ?-SMA was measured by Western Blot.[Result]After 24 and 48 hours incubation,healing fib roblasts showed absorbency values of 0.49?0.080 and 0.53?0.07 respectively.II was found that healing fibroblasts proliferated faster than normal fibroblasts(P