Objective To observe and compar the clinical efficacy of “CIWUJIA” and “DANSHEN” on the chronic patients with hepatitis B.Methods The 122 patients with hepatitis B were statically divided into two groups.Based on the protection for liver,one-group patients are cured with “DANSHEN” injection,and the other group patients are cured with “CIWUJIA” injection.All the patients have been injected one time one day,and the clinic procedure lasted 4 weeks.Results For the patients with low grade HBV or moderate grade HBV,"CIWUJIA" injection has the same efficacy as that of “DANSHEN” injection.However,the “CIWUJIA” injection also can improve the sleep of patients.Conclusions The “CIWUJIA” injection has the same efficacy as that of “DANSHEN” injection for patients at low-grade or moderate-grade HBV levels.Furthermore,the “CIWUJIA” injection is better for the patients who have sleep impediment.

Objective To investigate the relations between the portal parameters of homodynamic and stages of liver fibrosis of chronic hepatic B patients.Methods Fibrosis parameters of blood serum of one hundred and two patients with chronic viral hepatic B were measured with Doppler ultrasonic technique.The inner diameters,blood flow velocity,blood flow volume of their portal veins were investigated.And,the pathological changes of liver tissues were examined.Results The blood flow velocity of portal vein correlates very well with the stages of liver fibrosis,and reduces with the development of the degree of liver fibrosis.The blood flow velocity of severe fibrosis patients is obviously smaller than that of mild patients,and is 28.4% slower compared stage 4 with stage 1(P

Objective To investigate the effect of nuclear factor-?B(NF-?B) on the regulation of cyclooxygenase-2(COX-2) and Caspase-3 in hippocampal neurons after traumatic brain injury(TBI) in rats,and explore the neuroprotective effect and the possible mechanism of progesterone(PROG) in hippocampal neurons after TBI.Methods Forty-five male Spraque-Dawley rats were randomly divided into 3 groups: sham-operated group(n=15),TBI group(n=15) and PROG-treated group(n=15,intraperitoneal injection of PROG 16 mg/kg in 1 and 6 h after injury).The rat model of TBI was duplicated with the improved Feeney's method.The rats were sacrificed in 24 h after injury and their brain was resected.Nissl staining,immunohistochemical staining and Western blot assay for NF-?B,COX-2 and Caspase-3 was used to observe the changes of positive cell numbers and protein levels in the hippocampal neurons.Results The numbers of immunoreactive neurons to NF-?B(24.0?2.5),COX-2(35.9?2.7) and Caspase-3(25.1?2.7) were significantly increased in the hippocampus at 24 h after TBI when compared with the positive neuron numbers of NF-?B(1.9?0.9),COX-2(1.5?0.7) and Caspase-3(1.8?0.8) in sham group.After the treatment of PROG,the positive cell number of NF-?B(14.2?1.8),COX-2(16.6?2.7),Caspase-3(11.2?2.4) was reduced obviously as compared with the TBI group(P

ObjecUve To provide anatomical basis for diagnosis and therapeutic methods for treating grea-ter occipital nerve entrapment syndrome.Methods With 10 multiples microscope,the trace,distribution,compres-sion and relationship with occipital vessel of greater occipital nerve were observed and measured on 60 specimens of adult corpse.With a vernier caliper the distance of the easily compressed part of greater occipital nerve with external occipital protuberance,mastoidal and superior nuchal line were measured,and the superficial projeetion of the easily compressed part was marked.Results The course of the nerve could be divided into two parts:active part and inac-tive part.The former laid in the nuchal muscles,the latter ran and anchored to superficial fascia of the scalp,and easily compressed,accompanying with occipital vessel.This point lay in medial to occipital vessel and lateral to ex-ternal occipital protuberance(27.60±5.20)mm,and inferior to superior nuchal line(18.46±5.12) mm,and the superficial projection lay in median and superior 1/3 of the line from external occipital protuberance to mastoid apex. Conclusion Treating the greater occipital nerve compression syndrome by closed operation,the best position for needling lays in a bit inferior to point of median and superior 1/3 of the line from external occipital protuberance to mastoid apex.During the operation we should loose the main trunk compression of the greater occipital nerve as well as the branches compression on it.

Objective To investigate the different effects of different resection position of fibula on shape of tibiofibular syndesmosis,and explore the best position of cut fibula,providing reference for clinical surgeon to use fibula reasonably.Methods Ten adult male cadaverie specimens 172-176 cm long were used for 20 shank-ankle specimens.10 cm long fibula was cut proximally at the lower point 1/6,lower point 1/4,lower point 1/3,middle point 1/2 respectively,which was compared with the nornlal one to analyze the changes of shape of tibiofibular syndesmosis.Results Normally,the distance oftibiofibular syndesmosiswas(0.30±0.10)mm.Underthe condition of cut at the lower point 1/6,the distance of tibiofibular syndesmosis was enlarged[(0.54±0.20)mm](P＜0.05).In contrast,under the condition of cut 10 cm long fibula proximally at the middle point 1/2.the distance of tibioffbu1ar syndesmosis hadlittle effect[(0.31±0.20)mm](P＞0.05).Conclusion The best resection position of fibula is in the proximity of the fibula at the point 1/2.

Objective To analyze relationship between the gene polymorphism of CYP4F2 and essential hypertension (EH) in China's Han population. Methods 189 EH patients and 187 age-matched controls were used to analysis G20597A polymorphism site of CYP4F2 gene with polymerase chain-restriction fragment length polymorphisms. Results There was no difference of neither genotype nor allele of CYP4F2 (G20597A) between EH and controls through stratified analysis on gender. CYP4F2 gene 20597 G allele carriers had significant association with EH for male in China (81.6% vs 71.3% , P =0.019) , but not with female. After adjustment for cardiovascular risk factors (including smoking, TC, age, genetype, BMI) , the hazard ratio for incident EH in male with CYP4F2 20597GG increased about 2. 689 than that with CYP4F2 20597GA and 20597AA. Conclusion CYP4F2 20597G allele has significant association with male EH, and CYP4F2 20597GG may be an independent predictor of EH for male in china's Han population.

Objective To investigate the effect of progesterone on the expressions of inflammation-related factors of cortical cyclooxygenase-2 ( COX-2 ),prostaglandin E2 ( PGE2 ),inducible nitric oxide synthase (iNOS) and NF-κB in the cortex after traumatic brain injury (TBI) in rats so as to study the possible molecular mechanism of neuroprotective effect of progesterone on TBI.Methods Fortyfive male Spraque-Dawley rats were enrolled in the study and randomly divided into three groups,ie,sham operation group (n =15),TBI group (n =15) and progesterone treatment group (n =15).The rat model of TBI was duplicated with the improved Feeney' s method.The PROG treatment group was given i.p.injections of progesterone ( 16 mg/kg) at 1 and 6 hours after injury.The rats were sacrificed in three groups at 24 hours after injury and the specimens were removed.The changes of the positive cell numbers and protein level of COX-2,PGE2,iNOS and NF-κB in the cortex were examined by immunohistochemistry and Western blot.Results The positive cell numbers and protein levels of COX-2,PGE2,iNOS and NF-κB in the cortex of the TBI group were distinctly higher than those of the sham operation group (P＜O.05).While the positive cell numbers and protein levels of COX-2,PGE2,iNOS and NF-κB in the cortex of the progesterone treatment group were distinctly lower than those of the TBI group ( P ＜O.05).Conclusions Progesterone may exert protective effect on TBI through inhibiting NF-κB activity,blocking the inflammation response course of NF - κB and iNOS and decreasing the expressions of COX-2 and PGE2.

Objective The optimal community nursing intervention methods for community patients with primary hypertension was discussed in order to promote their health. Methods System management and community intervention were applied to 140 community patients with primary hypertension for 2 years, including strengthing community communication, popularizing health knowledge, improving cognition of danger of hypertension, hypertensive disease project management, tracking and monitoring the patients with primary hypertension and medication supervision.The cognition rate of prevention and control knowledge for hypertension, hypertension-related cardiovascular complication and formation rote of hypertension control behavior were compared before and after intervention. Results The prevalence rate of the knowledge on hypertension was increased, accompanied with promoting regular medication in patients with hypertension, decreasing cardiovascular complication caused by hypertension and heightening formation rate of controlling behavior in hypertension patients.Conclusions Applying community nursing intervention is practically effective in prevention and treatment of primary hypertension, deserving promotion and application in community medical institute.

Objective To evaluate the delivery of recombinant adeno-associated virus(rAAV)-mediated CD151 gene in promoting neovascularization and coronary collateralization in swines after myocardial infarction.Method Twenty-six chinese minipigs(clean,provided by breeding pig farm of Huazhong Agricultural University) were randomly divided into four groups:(1)normal control group(n=4):swines without surgery.(2)rAAV-GFP group(n=7):The acute myocardial infarction models were produced in swines by ligating the left anterior descending coronary artery(LAD).The success criteria of the models was that ST-segment elevations in leatds I and aVL maintained for 20 minutes.The rAAV-GFP was injected into the left ventricular anterior wall(divided into 10 points,1×10 11 pfu/point).(3)rAAV-CD151 group(n=7):The operation method was the same as rAAV-GFP group.The rAAV-CD151 was injected into the left ventrieular anterior wall(divided into 10 points).(4) rAAV-antiCD151 group(n=8):The operation method was the same as rAAV-GFP group.The rAAV-antiCD151 was injected into the left ventricular anterior wall(divided into 10 points).Eight weeks after coronary artery liga-tion,the expression of CD151 was measured by western blot.Coronary angiography was done to evaluate collateral circulation of the infarct zone of myocardium.The infurct size was determined by staining with triphenyl-tetrazolium chloride(TTC).Statistical analysis wan carried out by using one-way analysis of variances.Results High level of CD151 protein expression was detected.Coronary angiography showed better collateral circulation in the rAAV-CD151 group.The percentage of infarct size was sinificanly lower in the rAAV-CD151 group (12.82±2.26)% than that in the other two groups,and that was higber in rAAV-anti-CD151 group(32.52±3.47)% than that in the rAAV-GFP group(23.14%±2.83%,both P＜0.05).Conelusions CD151 in vivo gene transferred to swines with acute myocardial infarction promotes neovascularization and thereby improves collateral circulation.

Objective To investigate the effects of recombinant adeno-associated virus-mediated myostatin propeptide (MPRO) on uptake and oxidation of glucose,and glycogen synthesis in C2C12 myotubes,as well as the associated molecular mechanism.Methods Mature C2C12 myotubes were assigned to the following 6 groups:control,insulin,green fluorescent protein (GFP),insulin + GFP,MPRO,and insulin + MPRO groups.Glucose uptake,glucose oxidation,and glycogen synthesis were detected by counting radioactivity of 14CO2 or 14C labeled glycogen derived from 2-deoxy-[1-14 C] glucose.The activity of insulin signal pathway was evaluated by Western blot.Results Compared with control group,glucose uptake and glycogen synthesis were significantly increased in insulin and insulin+GFP groups,and further increased in insulin+MPRO group as compared with insulin alone(all P＜ O.05).However,MPRO and insulin had no effect on glucose oxidation.The phosphorylations of insulin receptor (IR) β,insulin receptor substrate 1 (IRS-1),protein kinase B (Akt),glycogen synthase kinase-3 β (GSK-3β),and the expressions of phosphatidylinositol 3-kinase (PI3K) and glucose transporter 4 (Glut4) in membrane were significantly increased in insulin and insulin+GFP groups compared with control group(all P＜0.05),and were further increased after MPRO transfection (all P ＜ 0.05).Conclusion MPRO may increase insulin-stimulated glucose uptake and glycogen synthesis in C2C12 cells by activating the IRS/PI3K/Akt signal pathway.