Objective:To analyze Jinxuan Zhike Xunxi powders ( JZX) and an active ingredients group ( AIG) obtained from JZX by GC-MS, and develop a characteristic fingerprint of AIG. Methods: A gas chromatography-mass spectrum ( GC-MS) method was applied to analyze the main compositions in JZX and AIG, and the characteristic fingerprint information in the fingerprint spectrum was determined. Results:Totally 10 batches of AIG were detected, and a promising GC-MS fingerprint spectrum containing characteristic information for AIG was obtained. Conclusion: The developed fingerprint with good repeatability can be successfully applied in the quality control of AIG.

Objective:To observe laxative function of Shutong digestion powder in mice with constipation by experimental means to provide reference for clinical research. Methods:ICR mice were used as the research objects,and the mice model of constipation was induced by oral administration of loperamide hydrochloride. Totally 120 ICR mice were divided into two groups(experimental group 1 and experimental group 2) with 60 in each,and each group was divided into 6 subgroups by random number:the normal control group, the model control group,the positive control group(mosapride citrate tablets,2.25×10 -3g·kg-1),Shutong digestion powder group respectively at low(1 g·kg-1),medium(3 g·kg-1) and high dose(9 g·kg-1) with 10 ones in each. Drugs were given by intra-gastric administration,once a day,for 7 days. In the first experiment group,mice were subjected to defecation test,and the stool traits and the time of first defecation in each group were observed and recorded. In the experimental group 2,small intestine motility test was conducted to observe and record the ink advancing rate in intestine. Results:Compared with the model control group,there was no sig-nificant difference in the body weight of mice in each Shutong digestion powder group(P>0.05),and the time for the first row of me-lena significantly decreased(P<0.05 or P<0.01). The rate of small intestine propulsion increased except for Shutong digestion pow-der group at low dose,and the other groups had significant differences (P<0.05 or P<0.01). Compared with the positive control group,there was no significant difference in the body weight and the first defecation time between Shutong digestion powder groups and the control group (P>0.05). The ink advancing rates of Shutong digestion powder groups were lower than that of the positive control group (P<0.05 or P <0.01). Conclusion:Shutong digestion powder has good promotion function for defecation and intestinal peri-stalsis.

Objective To investigate the potential mechanism for symptoms related to epigastric discomfort in patients without Helicobacter pylori (H.pylori) infection.Methods Patients who underwent gastric endoscopy and conformed H.pylori negative by histologic examination were enrolled.Among them,232 adult patients were collected between August 2006 and November 2006 and 31 children were collected between September 2005 and August 2009.All patients showed no apparent abnormality by endoscopic examination.The endoscopic biopsy was examined with HE or Warthin-Starry staining.Results In adult group,arteriole obstruction was found in 16 (8.8%) cases and focal haemorrhage in 82 (45.6%) cases.Both were existed in 82 (45.6%) cases.The histopathologic findings showed that arteriole obstruction in transition zone (65.2 %,P = 0.159) was common,whereas the focal haemorrhage in gastric fundus and corpus (65.6%,P=0.001) was in predominance.The symptom of heartburn was less in patients with arteriole obstruction and/or focal haemorrhage in comparison with those without these changes (x2 =8.564,P=0.003).In adolescent group,arteriole obstruction and/or focal haemorrhage accounted for 96.8% (30/31).Conclusion Gastric mucosa ischemic resulted from arteriole obstruction are commonly seen in both adult and adolescent patients,which is an important reason for epigastric discomfort in H.pylori negative patients.

OBJECTIVETo study the chemical constituents of Macrothelypteris viridifrons and their anti-proliferative effects on tumor cell.

METHODThe compounds were isolated by column chromatography with silica gel, C18 reverse-phase silica gel, sephadex LH-20, and their structures were elucidated on the basis of physiochemical propertities and spectral analysis. The antitumor activities of all compounds were tested with MOLT4, Hep G2, A-549, MCF-7, HT-29, PC-3 tumor cell lines.

RESULTFive compounds were isolated and identified as protoapigenone (1), protoapigenin (2), protoapigenin-4'-O-beta-D-glucopyanoside (3), 5,7-dihydroxy-2-(1,2-isopropyldioxy-4-oxo-cyclohex-5-enyl) -chromen-4-one (4), 5,7-dihydroxy-2-(1-hydroxy-2,6-dimethoxy-cyclohex-4-oxo) -chromen-4-one (5), respectively.

CONCLUSIONAll compounds were obtained from this plant for the first time. Compounds 1, 4 and 5 showed strong anti-proliferative effects on six tumor cells, which were in concentration-dependent manner.

Antineoplastic Agents ; chemistry ; pharmacology ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Ferns ; chemistry ; drug effects ; Flavonoids ; chemistry ; pharmacology ; HT29 Cells ; Hep G2 Cells ; Humans