1.Forensic pathological analysis on 23 cases of fatal pulmonary thromboembolism.
Dao-yin GONG ; Yao-bin WANG ; Cen-cen ZHANG ; Fei-jun HUANG
Journal of Forensic Medicine 2010;26(1):33-36
OBJECTIVE:
To analyze the forensic pathological characteristics of sudden death caused by pulmonary thromboembolism and the chronological transformation of thrombus and explore the assessment method of the causal relationship between previous trauma and the following fatal PTE episode.
METHODS:
All the 23 cases reviewed here were collected from our institute files from the year of 1998 to 2008.
RESULTS:
Trauma, surgery and braking etc. were all risky factors of PTE. Of these cases, 12 cases were caused by trauma, 21 cases were caused by surgery and 22 cases died in hospitals which were often happened one or two weeks after injury or one week's postoperative time. Of all the cases, 6 cases had single attack of thrombus and the rest 17 cases had the recurrence of thrombus. The number of the leg deep vein to be the embolic source was 16 cases which were often seen in the left leg.
CONCLUSION
It is important to confirm the embolic source, trauma, surgery and chronological events in determing the sudden death with PTE.
Adolescent
;
Adult
;
Aged
;
Autopsy
;
Cause of Death
;
Child
;
Death, Sudden/etiology*
;
Expert Testimony
;
Female
;
Forensic Pathology
;
Humans
;
Leg/blood supply*
;
Male
;
Middle Aged
;
Pulmonary Artery/pathology*
;
Pulmonary Embolism/pathology*
;
Retrospective Studies
;
Risk Factors
;
Venous Thrombosis/pathology*
;
Wounds and Injuries/complications*
;
Young Adult
2.Analysis of death caused by postpartum hemorrhage.
Xiao-yan HE ; Fei-jun HUANG ; Dao-yin GONG ; Cen-cen ZHANG ; Dong WANG
Journal of Forensic Medicine 2009;25(1):9-11
OBJECTIVE:
To analyze the pathological characteristics and the death reasons due to postpartum hemorrhage, and to help to deal with the obstetrical medical tangles.
METHODS:
Thirty-two cases of death caused by postpartum hemorrhage encountered in our department since 1995 had been collected and retrospectively analyzed.
RESULTS:
Death caused by postpartum hemorrhage could be divided into single factor and multi-factor, with 81.25% due to single factor, 12.50% multi-factor, and 6.25% unknown reason. The single factors included uterine atony, retained placenta, placenta increta, laceration of the lower genital tract, and coagulation defects. The multi-factor included a combination of two or more factors mentioned above.
CONCLUSION
The causes of death due to postpartum hemorrhage should be analyzed according to the clinical characteristics of the postpartum hemorrhage and the autopsy examination.
Autopsy
;
Blood Coagulation Disorders/complications*
;
Cause of Death
;
Female
;
Forensic Pathology
;
Humans
;
Placenta, Retained
;
Postpartum Hemorrhage/etiology*
;
Pregnancy
;
Retrospective Studies
;
Uterine Inertia
3.Forensic Analysis of 72 Cases with Multiple Organ Dysfunction Syndrome Caused by Traffic Accidents.
Yao Bin WANG ; Dao Yin GONG ; Li Li WANG
Journal of Forensic Medicine 2020;36(4):525-530
Objective To investigate the epidemiological and forensic characteristics of multiple organ dysfunction syndrome (MODS) after severe trauma and explore the reference indexes for determining traumatic MODS. Methods In terms of the number of organs or systems involved in MODS, the number of failures of each organ or system, the first failing organ and the survival time after organ failure, 72 cases of MODS death caused by traffic accidents were retrospectively analyzed. The cases were divided into two groups according to the mean injury severity score (ISS). The t test was used to analyze the differences in the number of organs or systems involved in MODS in the two groups. Chi-square test was used to analyze the differences in the types of first failing organs and the differences between the two groups in the number of cases of organ or system failure involved in MODS. Wilcoxon signed-rank test was used to analyze the differences between the two groups in survival time of MODS after trauma. Kaplan-Meier survival curve was drawn and Log-Rank test was performed. Results The number of MODS involved organs or systems after trauma in ISS≤35 group was 3-5, and 2-4 in the ISS>35 group (P<0.05). The cases of MODS organ or system failure after trauma occurred more in brain and lung in the two groups. The first failing organ after trauma was mainly the lung or kidney. The median time of first organ failure after trauma was 2.00 d, the median survival time of MODS after trauma in ISS≤35 group was 6.00 d, and 2.33 d in ISS>35 group (P<0.05). The survival curve of ISS≤35 group was relatively high and declined gradually, while the survival curve of ISS>35 group was relatively low and the decline was steep (P<0.05). Conclusion The epidemiological and forensic characteristics of MODS caused by traffic accidents have certain specificity. The ISS and the forensic characteristics of MODS at ISS>35 can be used as reliable reference indexes for evaluation of the causal relationship among trauma, MODS and death.
Accidents, Traffic
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Humans
;
Injury Severity Score
;
Multiple Organ Failure/etiology*
;
Retrospective Studies
;
Sensitivity and Specificity
;
Wounds and Injuries/complications*
4.Establishment of hepatocellular carcinoma multidrug resistant monoclone cell line HepG2/mdr1.
Yong-Bing CHEN ; Mao-Lin YAN ; Jian-Ping GONG ; Ren-Pin XIA ; Li-Xin LIU ; Ning LI ; Shi-Chun LU ; Jing-Guang ZHANG ; Dao-Bing ZENG ; Jian-Guo XIE ; Jia-Yin YANG ; Lü-Nan YAN
Chinese Medical Journal 2007;120(8):703-707
BACKGROUNDThe multidrug resistance (MDR) associated with the expression of the mdr1 gene and its product P-glycoprotein is a major factor in the prognosis of hepatocellular carcinoma cell (HCC) patients treated with chemotherapy. Our study was to establish a stable HCC MDR cell line where a de novo acquisition of multidrug resistance specifically related to overexpression of a transgenic mdr1.
METHODSThe 4.5-kb mdr1 cDNA obtained from the plasmid pHaMDR1-1 was cloned into the PCI-neo mammalian expression vector, later was transferred by liposome to human hepatocarcinoma cell line HepG2. Then the transfected HepG2 cells resisting G418 were clustered and cultured and the specific fragment of mdr1 cDNA, mRNA and the P-glycoprotein (Pgp) in these HepG2 cells were detected by PCR, RT-PCR and flow cytometry, respectively. The accumulation of the daunorubicin was determinated by flow cytometry simultaneously. The nude mice model of grafting tumour was established by injecting subcutaneously HepG2/mdr1 cells in the right axilla. When the tumour diameter reached 5 mm, adriamycin was injected into peritoneal cavity. The size and growth inhibition of tumour were evaluated.
RESULTSThe mdr1 expression vector was constructed successfully and the MDR HCC line HepG2/mdr1 developed. The PCR analysis showed that the specific fragment of mdr1 cDNA in HepG2/mdr1 cells, but not in the control group HepG2 cells. Furthermore, the content of the specific fragment of mdr1 mRNA and Pgp expression in HepG2/mdr1 cells were (59.7 +/- 7.9)% and (12.28 +/- 2.09)%, respectively, compared with (16.9 +/- 3.2)% and (3.07 +/- 1.06)% in HepG2 cells. In the nude mice HCC model, the tumour genes of both groups were identified. After ADM therapy, the mean size of HepG2 cell tumours was significantly smaller than HepG2/mdr1 cell tumours.
CONCLUSIONThe approach using the transfer of mdr1 cDNA may be applicable to the development of MDR hepatocarcinoma cell line, whose MDR mechanism is known. This would provide the experimental basis of MDR research.
ATP-Binding Cassette, Sub-Family B, Member 1 ; genetics ; metabolism ; Animals ; Carcinoma, Hepatocellular ; drug therapy ; genetics ; pathology ; Cell Line, Tumor ; Doxorubicin ; pharmacology ; therapeutic use ; Drug Resistance, Multiple ; genetics ; Drug Resistance, Neoplasm ; genetics ; Female ; Flow Cytometry ; Genetic Vectors ; genetics ; Humans ; Liver Neoplasms, Experimental ; drug therapy ; genetics ; pathology ; Mice ; Mice, Nude ; Mitomycin ; pharmacology ; therapeutic use ; Reverse Transcriptase Polymerase Chain Reaction ; Xenograft Model Antitumor Assays ; methods
5.Protective Effect of Compatibility of Coptidis Rhizoma and Magnoliae Officinalis Cortex on Rat Model of Ulcerative Colitis and Influence of Apoptosis
Xian-juan YANG ; Yin FU ; Jian WANG ; Hong-yan LI ; Li-ying WANG ; Jia-jun WANG ; Dao-yin GONG
Chinese Journal of Experimental Traditional Medical Formulae 2020;26(23):83-91
Objective:To study the protective effect of different doses of single-flavored Coptis, Magnoliae Officinalis Cortex, and their compatibility on ulcerative colitis (UC) model rats and the colonic B lymphoblastoma-2 associated X protein (Bax) and cysteine-containing aspartame-3(Caspase-3) protein, inflammatory cytokines, and other expressions. Method:The 120 healthy adult SD rats were randomly divided into blank group, model group, sulfasalazine group, Coptidis Rhizoma 2.00, 1.00, 0.50 g·kg-1 group, Magnoliae Officinalis Cortex 2.00, 1.00, 0.50 g·kg-1 group, Coptidis Rhizoma combine with Magnoliae Officinalis Cortex 4.00, 2.00, 1.00 g·kg-1 group, 12 groups with 10 rats in each group. The UC model was prepared by 2,4, 6-trinitrobenzene sulfonic acid/ethanol (TNBS/ethanol). After 24 h of modeling, the rats were gavaged at 10 mL·kg-1 for one time/d. After modeling, the mental state, activity state, hair luster, stool characteristics, and blood in the stool of each group were observed. After continuous administration for 6 days, colon tissues and spleen were taken after the last administration for 24 h. The ratio of colonic weight to length and spleen index was calculated. The degree of colonic injury was evaluated according to the colonic mucosal injury index (CMDI) score criteria. the histopathological observation was performed using hematoxylin-eosin staining (HE). The expression levels of tumor necrosis factor (TNF)-
6.Cardioprotective Effect of Three Borneol Prevention and Administration on AMI Model Rats
Ya-mei FAN ; Li-ying WANG ; Jian WANG ; Ying-rui ZHANG ; Yin FU ; Xian-juan YANG ; Xiao-qing GUO ; Dao-yin GONG
Chinese Journal of Experimental Traditional Medical Formulae 2020;26(6):64-72
Objective::The effects of three different doses of borneol on acute myocardial infarction (AMI) model rats and the effects on oxidative stress factors were compared to provide reference for elucidation of the dose-effect relationship and mechanism of anti-myocardial infarction. Method::Healthy adult male SPF SD rats were randomly divided into sham operation group, model group, solvation model group, nitroglycerin group, Borneolum high, medium and low dose(0.6, 0.3, 0.15 g·kg-1) group, l-Borneolum and Borneolum syntheticum high, medium, low dose(0.2, 0.1, 0.05 g·kg-1) group, a total of 13 groups, 20 in each group. Gavage was performed at 20 mL·kg-1 once a day for 3 days of continuous preventive administration. The sham operation group and the model group were given the same volume of distilled water, and the solvation model group was given the same volume of 5% polysorbate 80.On the third day of the pre-administration, 30 minutes after the last dose, the left anterior descending coronary artery was ligated to make a model, and the successful rats were treated for 3 days. BL-420N biological system analyzer was used to record the ST-segment amplitude and hemodynamic changes. Rat body weight and cardiac weight were weighed to calculate cardiac viscera coefficients, 2, 3, 5-triphenyl tetrazolium chloride (TTC) staining was used to calculate the myocardial infarction rate. Hematoxylin-eosin (HE) staining was used to evaluate the degree of myocardial pathological damage. According to the kit requirements, serum levels of lactate dehydrogenase (LDH), aspartate amino-transaminase (AST), creatine kinase isoenzyme (CK-MB) and oxidative stress factors superoxide dismutase (SOD), malondialdehyde (MDA) were detected. Result::Compared with the sham operation group, the ST segment amplitude of the model group significantly increased after 5 minutes, the left ventricular diastolic blood pressure (LVDP) value increased significantly, and the measured maximum shortening velocity (Vpm) value of the left ventricular myocardial contraction component significantly decreased. The organ coefficient and myocardial infarction rate were extremely significantly increased, and the myocardial pathological tissue was severely damaged. The serum CK-MB, AST, LDH, and MDA contents were significantly increased (
7.Effect of Yuanzhisan on Learning and Memory Abilities and Food Intake of AD Rats Based on Ghrelin Level of Cerebrointestinal Peptide
Yan-wei HAO ; Bin LI ; Yi ZHANG ; Pei-jun XIE ; Jun-rong YU ; Jin-song XIANG ; Dao-yin GONG
Chinese Journal of Experimental Traditional Medical Formulae 2020;26(21):8-14
Objective:Through the preparation of Alzheimer's disease (AD) rat model, the effect of Yuanzhisan on the expression of Ghrelin was observed, and the possible mechanisms in preventing and treating AD were discussed. Method:A total of 120 SD rats were randomly divided into sham-operated group, model group, donepezil group(1.02 mg·kg-1), and high, medium and low-dose Yuanzhisan groups (12,6,3 g·kg-1), with 20 rats in each group, including half male and half female. The rats in sham-operated group were injected with normal saline (NS), and the rats in other groups were injected with
8.Establishment of HPLC fingerprint and comparison of active ingredients between varieties of chrysanthemum in Hubei province.
Yin LIU ; Wen-Ling GONG ; Wu-Zhou BAO ; Lan-Ping GUO ; Yang XU ; Yi-Mei LIU ; Da-Hui LIU
China Journal of Chinese Materia Medica 2019;44(17):3711-3717
In this paper,the fingerprint of different varieties of chrysanthemum were established with " Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica" and the content of chlorogenic acid,galuteolin and 3,5-O-dicaffeoylquinic acid in 29 batches of different varieties of chrysanthemum in Futianhe town,Huangtugang town and Wuhan city were compared. At the same time,similarity evaluation and common peak clustering analysis were carried out. There were 11 common peaks in the fingerprints of 29 batches of different varieties of chrysanthemum,and the similarity ranged from 0. 802 to 0. 975. Hangju and Gongju were divided into one group by cluster analysis,and Huangju into another category. The established fingerprint method provides a basis for the identification of chrysanthemum cultivars. The content of 29 batches of chlorogenic acid was between 4. 092 and 11. 723 mg·g-1,luteolin was between 1. 010 and 11. 713 mg·g-1,and 3,5-O-dicaffeoylquinic acid was between 8. 828 and 33. 435 mg·g-1,both reach the pharmacopoeia standard,but the effective components of different varieties of chrysanthemum were quite different. Based on the contents of three active ingredients and the diversity of fingerprint peaks,the quality of the characteristic germplasm resource of local Fubaijuin Macheng is superior,and the protection of local characteristic germplasm resource should be strengthened in production.
Chlorogenic Acid
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analogs & derivatives
;
analysis
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Chromatography, High Pressure Liquid
;
Chrysanthemum
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chemistry
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Luteolin
;
analysis
;
Phytochemicals
;
analysis
9.Analysis and Evaluation of Mineral Elements in Gastrodia elata with Different Specifications and Grades from Diverse Producing Areas
Hong-yuan YAN ; Wen-ling GONG ; Yin LIU ; Tao ZHOU ; Lan-ping GUO ; Hua-sheng PENG ; Shuang-ying GUI ; Da-hui LIU
Chinese Journal of Experimental Traditional Medical Formulae 2021;27(12):147-156
Objective:To study the distribution characteristics of mineral elements in
10. Sensitizing effect of d-borneol on cisplatin-resistant NSCLC based on transcriptomics and its mechanism
Jin-Xiu LI ; Jia-Jun WANG ; Rong MA ; Qian XIE ; Jian WANG ; Nan ZENG ; Jin-Xiu LI ; Jia-Jun WANG ; Rong MA ; Qian XIE ; Jian WANG ; Nan ZENG ; Dao-Yin GONG
Chinese Pharmacological Bulletin 2023;39(6):1105-1114
Aim To explore the key targets of d-borneol combined with eisplatin for sensitization of cisplatin-resistant NCSLC cells by RNA-Seq and verify its mechanism. Methods Cisplatin-resistant human large cell lung cancer cells (H460/CDDP) were inoculated into the right armpit of male BALB/c nude mice (4 weeks old) to construct a xenograft tumor model. Then they were randomly divided into control group, vehicle group, eisplatin group, and combination group (d-borneol + eisplatin) with 6 nude mice and treated for 14 d. After last administration of 24 h, the tumor tissue was taken for RNA-Seq. And then real-time reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC) were used to verify the expression of cell cycle-related molecules. Results RNA-seq analysis showed that there were significant differences in gene expression between the eisplatin group and combined group, and they were significantly enriched in cell cycle. RT-PCR and IHC results showed that d-borneol combined with eisplatin could significantly inhibit the expressions of cyclins (cyclin A2, cyclin D3) and cyclin-dependent kinases (CDK2, CDK6) and promote the expression of its upstream molecular cyclin-dependent kinase inhibitor CD-KI (P21, P27) (P<0. 05, P<0.01). Conclusions d-Borneol increases the sensitivity of eisplatin by increasing the expression of P21 and P27 and inhibiting the expression of cyclinA2/D3 and CDK2/6 to induce cell cycle arrest and inhibit the malignant proliferation of H460/CDDP cells, thereby achieving the effect of anti-drug sensitization.