Objective To analyze the safety of methimazole in the treat-ment of hyperthyroidism before menopause and its effect on regulatory T cells( Treg) , cytokines of CD4 +CD25 +.Methods A total of 164 pa-tients with hyperthyroidism were randomly divided into treatment group and control group , 82 cases in each group.Patients in treatment group were orally given methimazole 10 mg, tid, after 30 d reduced to bid, 10 mg each time, after another 15 d, reduced to qd, 15 mg each time.Pa-tients in control group were orally given propylthiouracil 100 mg, tid, af-ter 30 d reduced to bid, 100 mg each time, after another 15 d, reduced to tid, 50 mg each time.All patients were treated for 3 months.The thy-roid serological index , CD4 +CD25 + regulation T cells and cytokines levels between the two groups before and after treatment were compared.The incidences of adverse drug reactions in two groups were also observed.Results The thyroid stimulating hormone ( TSH ) , thyrotrophin receptor antibody ( TRAb ) significantly increased in two group ( P <0.05 ).The free triiodothyronine (FT3), free thyroxin(FT4) and thyroid peroxidase antibody(TPOAb) in two groups were significantly decreased.The TSH, FT3 ,and FT4 were improved more significantly in treatment group ( P<0.05 ).There was no significant change in the number of CD4 +T cells in the two groups before and after treatment (P>0.05).The number of CD4 +CD25 +Tregs cells, IL-6 and IL-10 levels significantly decreased , and treatment group were significantly lower than control group ( P<0.05 ).The incidence of adverse reactions was 2.4% in treatment group , 6.1% in control group ( P>0.05 ).The total effective rate of treatment group was 97.6%, significantly higher than 81.7% in control group ( P<0.05 ).Conclusion Compared with propylthiouracil , methimazole had better effect on thyroid hormone level and immune function in premenopausal hyperthyroidism patients.

OBJECTIVETo evaluate the psychological situations of patients with soft tissue injuries in oral and maxillofacial region by different kinds of suturing.

METHODSA total of 200 patients were selected and randomly divided into two groups. Group A received intradermic suture while group B underwent para-position suture. All patients were evaluated by hospital anxiety and depression (HAD) scales pre-suture, after one week, one month and three months.

RESULTSThe HAD total scores of group B were significantly high compared with group A (P < 0.05) after one week and one month, while there was no difference between group A and group B pre-suture and three months later.

CONCLUSIONSIntradermic suture results in less psychological influence in patients with soft tissue injuries in oral and maxillofacial region.

Adult ; Anxiety ; Depression ; Face ; surgery ; Humans ; Maxillofacial Injuries ; psychology ; surgery ; Soft Tissue Injuries ; psychology ; surgery ; Suture Techniques

Superoxide dismutase (SOD) is a key enzyme that scavenge superoxide anion free radical (O₂^·-) in vivo, and plays an important role in plant growth and development and stress. In this study, according to the genome and transcriptome data of Salvia miltiorrhizae, 9 SOD genes were identified and the expression patterns of SOD family genes were further analyzed, including 5 Cu/Zn-SOD, 2 Fe-SOD and 2 Mn-SOD. On the basis of proteomic analysis, combined with transcriptome data, one full-length cDNA of Mn-SOD gene, namely SmMSD2 was cloned from Salvia miltiorrhizae. The results of amino acid sequence alignment and phylogenetic analysis showed that SmMSD2 protein belongs to the manganese superoxide dismutase (Mn-SOD) subfamily, and SmMSD2 protein shares high sequence identity with the Mn-SOD proteins of various plants that all contain a C-terminal conserved metal-binding domain "DVWEHAYY". The prokaryotic expression vector pMAL-c2X-SmMSD2 was constructed and transformed into E. coli BL21 expressing strain, and the target recombinant protein was successfully induced and its enzymatic properties were analyzed. Spatiotemporal expression analysis showed that SmMSD2 gene was expressed in all tissues, indicating that SmMSD2 gene was constitutively expressed at a stable level. Real-time quantitative PCR indicated that drought (15% PEG6000), abscisic acid (ABA) and indole-3-acetic acid (IAA) could induce the expression of SmMSD2 gene, suggesting that SmMSD2 may be involved in the response of Salvia miltiorrhizae to abiotic stress such as drought, as well as the signaling pathways of phytohormone ABA and IAA. These results lay the foundation for further elucidating the involvement of superoxide dismutase in the stress response and accumulation of active components of Salvia miltiorrhiza.