1.Interaction between polymorphisms in NQO1C609T and XRCC1G28152Aand their correlation with smoking on gastric cancer
Dao-Jun CHEN ; Rui DING ; Wei CAO ; Dong-Qing YE
Chinese Journal of Epidemiology 2011;32(1):5-8
Objective To investigate the relationship between polymorphism of NAD (P)H quinone oxidoreductase 1 (NQO1)and X-ray repair cross-complementing group 1 (XRCC1) and their correlation with smoking on the susceptibility to gastric cancer. Methods A 1:1 case-control study of 334 patients with primary gastric cancer, with non-cancer or alimentary inpatients as control group (matched for ages ± 5 years, sex and reqion) in Anhui province was conducted to analyze theNQO1C609T and XRCC1G28152A. Gene types by PCR-based restriction fragment length polymorphism techniques. Interaction index (γ) was calculated to determine the type of gene- environment interaction. Results The average age of 334 cases of gastric cancer patients was 57 years, with 65.3% of them were male. Smoking rate in the case group (55.09%) was significantly higher than in the control group (36.53%). The consequence showing that it carried the heterozygous variant (CT)or homozygous variant (TT) of NQO1 could enhance the risk of gastric cancer(OR= 1.507,3.050),but not the XRCC1G28152A gene polymorphism or the susceptibility to gastric cancer. At the same time,individuals that carrying XRCC1AG and NQO1TT could increase 2.789 times the incidence of gastric cancer than those who carrying the XRCC1AG or NQO1CC. The gastric cancer risk of XRCC1GG individuals that carrying NQO1TT was 4.448 times higher than those who carrying XRCC1GG or NQO1 CC. The positive interactions of NQO1 homozygous variant (TT) , XRCC1 homozygous variant (GG) and smoking were revealed in the occurrence rates of gastric cancer (OR=3.094,γ =2.070). Conclusion Our research findings showed that the significant interactions between genetic polymorphisms of NQO1, XRCC1 and smoking added the risk of gastric cancer, while genetic and environmental hazardous factors co-effecting the development of gastric cancer.
2.Study on Clinical Consistency Evaluation in TCM Practice Guidelines for Prevention and Treatment of Biqiu (Allergic Rhinitis)
Hao XU ; Hong-Jun DONG ; Xu-Qing CHEN ; Dao-Nan YAN
Chinese Journal of Information on Traditional Chinese Medicine 2018;25(3):16-18
Zhangjiagang Hospital of Traditional Chinese Medicine, as a collaborative unit of the project, participated in the clinical consistency evaluation in project of TCM Practice guidelines for Prevention and Treatment of Biqiu (allergic rhinitis) organized by the State Administration of Traditional Chinese Medicine. The evaluation results showed that the guideline met the clinical practice requirements. This article summarized the clinical evaluation experience from the three aspects: The diagnosis in TCM and Western medicine is clear and definite, but the English translation needs to be considered; The definition of remission phase of Biqiu (allergic rhinitis) is difficult, to interfere by identifying the constitution is innovative; The advantages of "preventive treatment of diseases" in TCM should be fully played in role, improving the satisfaction of patients.
3.Clinical observation on acupuncture for diarrhea-predominant irritable bowel syndrome patients in syndrome of liver-stagnation and spleen-deficiency and its impact on Th1/Th2.
Xiao-Liang WU ; Ye-Lin WANG ; Jian-Hua SUN ; Yan-Ye SHU ; Li-Xia PEI ; Jun-Ling ZHOU ; Dong CHEN ; Ji-Wei ZHANG ; Dao-Wei ZHAN
Chinese Acupuncture & Moxibustion 2013;33(12):1057-1060
OBJECTIVETo observe clinical therapeutic effects of acupuncture for pattern of liver-stagnation and spleen-deficiency in diarrhea-predominant irritable bowel syndrome (D-IBS) and its impact on cell factors.
METHODSForty cases were selected and divided into an acupuncture group (21 cases) in which acupuncture was applied and a medicine group (19 cases) in which oral administration of dicetel and bifidobacterium lactobacillus triple viable capsules were applied. The symptom scores, level of Th1-type cytokine (IFN-gamma, IL-2) and Th2-type cytokine (IL-4, IL-10) and ratio of IFN-gamma to IL-4 were compared in two groups before and after treatment to analyze acupuncture effect.
RESULTSThe clinical symptoms were improved after one-week treatment in the acupuncture group (P<0.05), which had faster onset than the medicine group (P<0.05). The total effective rate was 90.48% (19/21) in the acupuncture group, which was superior to 78.95% (15/19) in the medicine group (P<0.05). Compared with medicine treatment, imbalanced condition of Th1/Th2 was turning towards the direction of Th2 after acupuncture, indicating a tendency to recover the balance.
CONCLUSIONThe clinical efficacy of acupuncture for D-IBS has close relationship with effectively improving balance of Th1/Th2 in patients with liver-stagnation and spleen-deficiency.
Acupuncture Therapy ; Adult ; Aged ; Cytokines ; immunology ; Female ; Humans ; Irritable Bowel Syndrome ; immunology ; physiopathology ; therapy ; Liver ; physiopathology ; Male ; Middle Aged ; Spleen ; physiopathology ; Th1 Cells ; immunology ; Th2 Cells ; immunology ; Young Adult
4.Breast intraductal lesion resection under breast fiberoptic ductoscopy.
Wei WU ; Xiao-rong LI ; Kai-yan YANG ; Bo-ni DONG ; Dao-jin CHEN
Journal of Central South University(Medical Sciences) 2008;33(1):81-84
OBJECTIVE:
To explore the feasibility and to sum up the experience of breast intraductal neoplasm resection under breast fiberoptic ductoscopy (FDS).
METHODS:
FDS was performed on 548 patients with nipple discharge from Sep.2004 to Nov.2006. The clinical data of breast intraductal neoplasm found by FDS in patients who underwent tumor resection were analyzed, and the breast intraductal neoplasm image characteristics, diagnosis, operative type and postoperative pathological results were analyzed.
RESULTS:
Of the 548 patients with nipple discharge, intraductal neoplasm was found in 187 cases (34.1%), intraductal papilloma in 159 cases (29.0%), intraductal papillomatosis in 12 cases (2.2%), and breast carcinoma in 16 cases (2.9%). One hundred thirty-five patients were operated on in our hospital, of whom 91 were performed tumor resection or segmentectomy under the localization by FDS, and the other 44 were performed segmentectomy after breast duct infusion of methylene blue. The diagnostic rate under FDS in the FDS group (97.8%) was higher than that in the breast duct infusion methylene group (86.4%) (chi2=6.96, P=0.008).
CONCLUSION
FDS is not only an accurate diagnosis for breast intraductal lesion, but also an assistance to localize the breast intraductal neoplasm and to remove them in the operation.
Adult
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Aged
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Aged, 80 and over
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Breast Neoplasms
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diagnosis
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surgery
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Carcinoma, Ductal, Breast
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diagnosis
;
surgery
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Endoscopy
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methods
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Female
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Fiber Optic Technology
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methods
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Humans
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Middle Aged
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Papilloma, Intraductal
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diagnosis
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surgery
5.Clinical study of the application of enhanced recovery after surgery in cervical spondylotic myelopathy.
Bin DAI ; Pei GAO ; Qi-Rong DONG ; Yu-Mei WANG ; Dong CHEN ; Yu-Cheng SHEN ; Bing ZHANG ; Zhi-Dong LIU ; Dao-Long LI
China Journal of Orthopaedics and Traumatology 2018;31(8):740-745
OBJECTIVETo evaluate the role of enhanced recovery after surgery in treating cervical spondylotic myelopathy.
METHODSThe clinical data of 55 patients with cervical spondylotic myelopathy underwent surgical treatment from January 2012 to December 2015 were retrospectively analyzed. There were 30 males and 25 females, age from 36 to 71 years old with an average of (45.2±3.2) years, course of disease was for 1 to 12 months with an average of (4.5±1.8) months. The concept of enhanced recovery after surgery and perioperative management were applied to surgical treatment in 35 patients (study group), and the same period, 20 patients without strategy of enhanced recovery after surgery (control group). Thirty-eight patients were treated by anterior cervical discectomy decompression and fixation(ACDF), 17 patients were treated by posterior single-open door laminoplasty decompression. The activity time out of bed, hospitalization days after surgery were compared between two groups. Japanese Orthopaedic Association (JOA) score and visual analogue score(VAS) before operation, after operation at 1, 7, 30 days and 6, 12 months was respectively used to evaluate the neurological function and pain.
RESULTSAll the patients were followed up for 12 to 18 months with an average of (14.3±1.5) months. There was no significant difference in age, gender, surgical methods, preoperative VAS, JOA score between two groups (>0.05). The activity time out of bed was 3 to 8 h with an average of (5.54±1.54) h, postoperative hospitalization time was 3 to12 d with an average of (5.62±1.59) d in study group, while in control group, the activity time out of bed was 24 to 48 h with an average of (18.80±4.78) h, and postoperative hospitalization time was 7 to 17 d with an average of (9.85±1.94) d; there was significant difference between two groups (<0.01). There was significant difference in VAS and JOA scores between two groups at 1, 7, 30 d after operation (<0.01), and there was no significant difference at 6, 12 months after operation(>0.05). There were no neurologic function deterioration, hematoma, wound infection, internal fixation loosening and other complications in study group during hospitalization and following-up;there were 2 cases of superficial wound infection in the control group, who healed by dressing change for 2 weeks;there was no significant difference between two groups(>0.05).
CONCLUSIONSThe strategy of enhanced recovery after surgery in treating cervical spondylotic myelopathy can promote the early recovery, shorten the length of stay and improve the patient's degree of satisfaction.
6.Study on fluvoxamine maleate sustained-release pellets and its compression technology
Ming-hui XU ; Xing-yue ZHANG ; Qiao DONG ; Xia ZHAO ; Yu-ru BU ; Le-zhen CHEN
Acta Pharmaceutica Sinica 2024;58(2):439-447
In this study, fluvoxamine maleate sustained-release pellet system tablets were prepared and were used to evaluate their release behaviors
7.HSC transplantation-associated intestinal thrombotic microangiopathy: clinical pathological features, diagnosis criteria and treatment.
Lu-Jia DONG ; Da-He XIE ; Dao-Pei LU ; Huan CHEN ; Zhi-Yong GAO ; Yu-Hong CHEN ; Tong WU ; Wei HAN ; Xiao-Hui ZHANG ; Yan-Li ZHAO
Journal of Experimental Hematology 2006;14(2):327-331
Thrombotic microangiopathy (TMA) is a lethal transplantation-associated complication which exactly likes acute intestinal graft-versus-host disease (GVHD) in the clinical manifestation. 373 consecutive patients with hematological diseases received family HLA matched or mismatched HCT from May, 2002 to July, 2004. To analyse the clinical and pathological characteristics of TMA, 30 patients who suffered from severe diarrhea and received colonoscopic examination and gut biopsy were retrospectively analyzed. The results indicated that 7 patients originally diagnosed as gut GVHD showed the pathological evidence of enteric TMA. The incidence of TMA was 7 out of 30 specimen (23.3%). Pathological evidence of enteric TMA shown microvascular disorder characterized by thrombus in the capillary without infiltration of lymphocytes and perivascular hemorrhages in the mucosa, swelling and focal denudation of epithelial cells. All patients with TMA were associated with cytomegalovirus (CMV) antigenemia/disease. Among these patients, 4 cases, who only showed TMA without the evidence of gut GVHD pathologically, displayed treatment-resistant bloody diarrhea, renal failure, veno-occlusive disease, hemorrhagic cystitis, hemolytic anemia as well as thrombocytopenia. But the other 3 cases, with co-existence of both TMA and GVHD pathological characteristics had better treatment response. Survival analysis indicated that 3 patients with TMA-GVHD survived for 461 to 536 days but three out of four TMA patients died from VOD with liver failure as well as multiple organ failure during 101 to 254 days after HCT. In conclusion, to better diagnose those patients with severe and refractory diarrhea following HCT, pathological examination may indicate crux evidence to identify intestinal TMA from gut GVHD. Furthermore, this primary report has first evidenced that TMA and TMA-GVHD are two pathologically well-recognized subtypes with the difference between the pathological characteristics, treatment response and clinical outcomes.
Graft vs Host Disease
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etiology
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Hematopoietic Stem Cell Transplantation
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adverse effects
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Humans
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Intestinal Diseases
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diagnosis
;
etiology
;
pathology
;
Purpura, Thrombotic Thrombocytopenic
;
diagnosis
;
etiology
;
pathology
;
Reference Standards
;
Retrospective Studies
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Thrombosis
;
diagnosis
;
etiology
;
pathology
8.Isolation and identification of rhizobacteria RS-3 from Panax ginseng and evaluation of its antagonistic activity.
Tie-Lin WANG ; Dong-Yue ZHAO ; Mei-Lan CHEN ; Zhong-Hua MA ; Lan-Ping GUO
China Journal of Chinese Materia Medica 2016;41(24):4564-4567
A rhizobacteria strain named RS-3 exhibited inhibitory activity against all five Panax ginseng pathogens was isolated from the root of P. ginseng. This strain was identified as Bacillus amyloliquefaciens based on its morphological character and 16S rDNA sequence. Antagonistic activity experiments indicated that the strain could strongly suppress Botrytis cinerea Pers with an inhibitory rate of 54.4%, suggesting the potentialities of biocontrol agent against diseases that frequently happen on ginseng.
9.Construction of quality evaluation strategy for Chinese medicine based on ambient mass spectrometry.
Chang-Jiang-Sheng LAI ; Zi-Dong QIU ; Xu-Ya WEI ; Ze-Yan CHEN
China Journal of Chinese Materia Medica 2022;47(1):279-284
Quality is the guarantee for the clinical safety and effectiveness of Chinese medicine. Accurate quality evaluation is the key to the standardization and modernization of Chinese medicine. Efforts have been made in improving Chinese medicine quality and strengthening the quality and safety supervision in China, but rapid and accurate quality evaluation of complex Chinese medicine samples is still a challenge. On the basis of the development of ambient mass spectrometry and the application in quality evaluation of complex Chinese medicine systems in recent years, the authors developed the multi-scenario Chinese medicine quality evaluation strategies. A systematic methodology was proposed in specific areas such as real-time monitoring of the quality of complex Chinese medicine decoction system, rapid toxicity grading of compound Chinese patent medicine, and evaluation of bulk medicinals of Chinese patent medicine. Allowing multi-scenario analysis of Chinese medicine, it is expected to provide universal research ideas and technical methods for rapid and accurate quality evaluation of Chinese medicine and boost the high-quality development of Chinese medicine industry.
China
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Drugs, Chinese Herbal
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Mass Spectrometry
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Medicine, Chinese Traditional
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Nonprescription Drugs
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Reference Standards
10.Relief of abdominal pain by morphine without altering physical signs in acute appendicitis.
Yong YUAN ; Jia-yong CHEN ; Hao GUO ; Yi ZHANG ; Dao-ming LIANG ; Dong ZHOU ; Hui ZHAO ; Feng LIN
Chinese Medical Journal 2010;123(2):142-145
BACKGROUNDAbdominal pain is a common symptom among patients with acute appendicitis, yet these patients have long been denied relief from suffering because of widespread misconceptions associated with the use of opioids. We determined whether morphine hydrochloride masked the physical signs in adults with acute appendicitis and assessed the efficacy of morphine in relieving abdominal pain.
METHODSA prospective, double-blind, placebo controlled, clinical trial was conducted with 106 adult patients between 16 and 70 years old with acute appendicitis. Patients were randomly divided into a morphine group (n=54) or a normal saline group (n=52). All patients presented with acute abdominal pain with onset within 3 days. The morphine group received hypodermic injection of morphine (0.15 mg/kg; maximum 20 mg) and the control group members were given an equivalent volume of normal saline solution. The clinical symptoms, physical signs, and patients' cooperation during physical examination were assessed before and after 30 minutes of morphine or normal saline administration.
RESULTSAbdominal pain was significantly relieved and the patients' cooperation was improved in the morphine group after 30 minutes treatment compared with the control group and before morphine administration (P<0.05). The physical signs were unaffected by either treatment (P>0.05).
CONCLUSIONSMorphine relieved abdominal pain and improved the patients' cooperation for treatment and care. Furthermore, the morphine did not mask the physical signs of acute appendicitis.
Abdominal Pain ; drug therapy ; pathology ; Adolescent ; Adult ; Aged ; Analgesics, Opioid ; therapeutic use ; Appendicitis ; drug therapy ; pathology ; Female ; Humans ; Male ; Middle Aged ; Morphine ; therapeutic use ; Young Adult